• Title/Summary/Keyword: leaf chlorophyll contents

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Changes of Chlorophyll and their Derivative Contents during Storage of Chinese Cabbage, Leafy Radish and Leaf Mustard Kimchi (배추, 열무 및 갓김치 저장 중의 Chlorophyll 및 그 유도체의 함량변화)

  • 이종호;김경업;이용숙;김성희;정효숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.5
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    • pp.852-857
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    • 1998
  • Three kinds of kimchi using Chinese cabbage, leafy radish and mustard leaf were prepared by conventional method and stored at 5$^{\circ}C$ or 2$0^{\circ}C$ for 13 dyas. During storage at both temperatures, changes of the amounts of salt and ascorbic acid, pH and total acidity were determined, and the relationship of the decomposition of chlorophylls with the production of their derivaties was studied. At both storage temperatures, salt concentration of Chinese cabbage kimchi(3.7%), leafy radish kimchi(3.6%), mustard leaf kimchi(3.5%) was relatively constant during the entire storage period. However, pH and total acidity wre fluctuating with the remarkable changes during 3 days of storage. Ascorbic acid content was slowly decreased during the storage period and the decompositin rate of ascorbic and was greater at 2$0^{\circ}C$ than 5$^{\circ}C$. Among the kinds of kimchi tested, mustard leaf kimchi with the slow decomposition rate of ascorbic acid contained relatively high ascorbic acid content, while leafy radish kimchi contained the lowest content. At both storage temperatures, the production of pheophytin and pheophorbide from decomposition of chlorophyll was least in mustard leaf kimchi, but similar production rates in leafy radish and Chinese cabbage kimchi were observed.

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Color Stability of Chlorophyll in Young Barley Leaf (새싹보리 클로로필의 색 안정화)

  • No, Junhee;Yoon, Huina;Park, Sara;Yoo, Seung Jin;Shin, Malshick
    • Journal of the East Asian Society of Dietary Life
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    • v.26 no.4
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    • pp.314-324
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    • 2016
  • To improve the color stability of chlorophyll in young barley leaf used as functional green biomaterial, the absorption spectrum, color values, and antioxidative activities of young barley leaf (YBL) treated with zinc ion solutions were investigated. The small pieces of fresh YBL in aqueous solution mixtures were autoclaved twice at $110^{\circ}C$ for 30 min (pH 5). Distilled water (BLA), 0.01% zinc chloride (BLAZ), 0.01% zinc citrate (BLAC), and 0.01% zinc lactate (BLAL) solutions were used. Treated YBL powders were extracted with 80% EtOH for 4 h. Chlorophyll a and b contents differed with different treatments. BLA decreased chlorophyll a and b contents, whereas others were maintained. Absorbance spectrums of chlorophyll at 400~700 nm showed different maximum peak wavelengths. After heating in acidic and neutral solutions (pH 3, 5, and 7), the colors of YBL and BLA changed from green to olive green, whereas BLAZ, BLAC, and BLAL remained green color. The antioxidative activities showed higher values in YBL extract than in treated extracts. From the above results, autoclaved YBL in zinc solution would increase the color stability and maintain green color regardless of acid and heat treatments.

Morphology and Chlorophyll Contents of Leaf and Wood Anatomical Characteristics of Three Schisandraceae Species in Korea (국내자생 오미자과(科) 3수종의 잎 형태, 엽록소 함량 및 목부해부학적 특성)

  • Kim, Sea-Hyun;Lee, Keb-Yean;Baik, Eul-Sun;Han, Jin-Gyu;Kang, Moon-Su
    • Korean Journal of Plant Resources
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    • v.23 no.1
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    • pp.31-37
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    • 2010
  • It was investigated that morphological characteristics of leaf and stomata, chlorophyll contents and wood anatomy characteristics in Schisandra chinensis, Schisandra nigra and Kadsura japonica. Leaf morphological characteristics were significant at 5% levels within the species and within the individuals as a male, a female and monoecious. K. japonica was the longest in the leaf length, followed by S. chinensis and S. nigra. For the leaf length among S. nigra individuals, the female was longer than male and monoecious. As for the Petiole length, S. nigra was twice longer than S. chinensis and K. japonica. Stomata length was ranged from $92.5\;{\mu}m$ to $105.9\;{\mu}m$ for S. nigra, $79.9\;{\mu}m$ for S. chinensis, $76.2\;{\mu}m$ for K. japonica. S. nigra was longer than the others in length and width, while its stomata density was lower than the others. The size of vessel elements and xylem fiber showed a similar tendency to stomata, significant at 5% levels within S. nigra individuals (male, female and monoecious). Chlorophyll contents were also variable in inter and intra species and in general K. japonica showed higher levels of chlorophyll contents than Schisandra spp. plants.

Nondestructive and Rapid Estimation of Chlorophyll Content in Rye Leaf Using Digital Camera

    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.41-45
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    • 2004
  • We have developed and tested a new method for nondestructive estimation of chlorophyll- and nitrogen-contents in rye leaf. It was found that the relation-ships among nitrogen, chlorophyll content and fresh weight were significantly positive correlated. Nitrogen and chlorophyll content were positively correlated whereas correlation coefficients among R, G, R-B and G-B on the basis of photo-numerical values were negative. We have found that R/(R-B) obtained from data of digital camera is the best criterion to estimate the chlorophyll content of leaves. The regression curves of the relation between R/(R-B) and chlorophyll content were also calculated from the data collected on cloudy days. The coefficients of determination ($\textrm{r}^2$) were ranged from 0.33 to 0.99. In this study, the accuracy in estimating chlorophyll content from the color data of digital camera image could be improved by correcting with R, G, and B values. It is suggested that, for practical purposes, the image values estimated with sufficient accuracy using a portable digital camera can be applied for determining chlorophyll content and nitrogen status in plant leaves.

The simple assay of phosphinothricin acetyltransferase gene on the transgenic potato (형질전환 감자에서 제초제 저항성 유전자인 PAT gene의 간편한 확인)

  • 정재훈;양덕춘;방극수;최경화;한성수
    • Korean Journal of Plant Resources
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    • v.12 no.4
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    • pp.253-259
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    • 1999
  • In this study, three simple methods were established to confirm the transgenic potato plants. The leaf disc was used in the first method. After leaf discs of transgenic and non-transgenic potato were transfered into the liquid MS medium with bialaphos 5mg/l, 25 days, the chlorosis occurred in the non-transgenic leaf discs while it could not find in the transgenic leaf discs, In the second method, shoot tips of potato were transferred into MS medium supplemented with 0.5mg/l bialaphos and 0.6% agar. After 7-10 days, a lot of roots developed from the transgenic shoot tip, but the non-transgenic shoot tip was dead. The third method was using chlorophyll contents. Leaf discs were transferred into the liquid MS medium with bialaphos 0.5 mg/l. After 15 days, the content of chlorophyll A in transgenic plant was at least 2.5 times higher than in non-transgenic plant. In addition, the PAT enzyme activity were detected in the transgenic potato, but not detected in normal potato.

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Leaf Chlorophyll Contents of Three Populus (Salicaceae) Species Occurring on Elevational Gradient (표고 구배상에 분포하는 Populus 속 ( 버드나무과 ) 3 종 식물 잎의 엽록소 함량)

  • Cha, Young-Il
    • The Korean Journal of Ecology
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    • v.10 no.3
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    • pp.119-127
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    • 1987
  • On the east slope of the front ranges of the Colorado Rockies, USA, three species of Populus are distributed at different elevations from the Great Plains to the timberline. At elevations 1, 720m (P. sargentii), 2, 335m (P.angustifolia) and 3, 190m (P.tremuloides) chlorophyll cotents of leaves of thses dioecious trees were determined. Total chlorophyll content was in the range of 0.86-1.56mg/g leaf, increasing with elevation as a second-degree polynomial, and the peak content was estimated to be reached at about 2, 800m. Total chlorophyll content was greater in female than in male trees in the two higher elevation species, but not in P. sargentii, the low elevation species. Chlorophyll b content was greater than chlorophyll a content in the same two higher elevation species. The increase of chlorophyll b than to increase of chlorophyll a content. These phenomena appear to be the result of adaptation of these species to different environmental conditional at different elevation. Sequential distribution of plant populations on environmental gradients in such a way that variation in given plant characters paralell the environmental gradient must occur not only in ecotypes of a species, but also in different but closely related species.

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Effects of Leaf and Pod Removal on Photosynthesis and Assimilate Partition in Soybean (적엽ㆍ제협처리가 콩의 광합성과 동화물질 배분에 미치는 영향)

  • Woong Tae, Kim;Rak Chun, Seong;Harry C, Minor
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.159-165
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    • 1993
  • To clarify the effects of sink demand for assimilate on leaf photosynthetic rate, tissue composition, and leaf senescence of soybean [Glycine max (L.) Merr.] J plants, pod and leaf tissues were removed at growth stage $R^3$. Plant responses were measured every 10days from 2 through 42days following treatment. Leaves of depodded plants exhibited increased starch and chlorophyll contents and specific leaf weight. Stomatal resistance was also increased and leaf photosynthetic rate was reduced. Dry weight of vegetative tissues except leaves was increased by pod removal. Leaf removal resulted in a decreased starch content of leaves from 22 to 42days after treatment and that of roots at all sampling times. Specific leaf weight was decreased while leaf photosynthetic rate was increased. Stomatal resistance and chlorophyll content were little affected. Weight per seed was decreased 3.0% by leaf removal. Except for the seed, tissue protein contents were increased by pod removal but decreased by leaf removal, however, seed protein content was not affected by either. Apparent senescence was delayed by depodding. Both apparent and functional senescence were accelerated by leaf removal.

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Physiological and Biochemical Responses of Fifteen Rice Cultivars to UV-B Radiation

  • Sung Jwa-Kyung;Chung Jong-Wook;Lee Sang-Min;Lee Yong-Hwan;Choi Du-Hoi;Kim Tae-Wan;Song Beom-Heon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.1
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    • pp.39-44
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    • 2005
  • This study was conducted to examine the physiological and biochemical responses against UV-B radiation in the seedling of 15 different rice cultivars, having the different physiological sensitivities. Out of 15 rice cultivars tested, moderate and susceptible groups showed significant decreases in biomass and RGR (relative growth rate). Contents of total chlorophyll were reduced remarkedly by irradiation of UV-B. In all rice cultivars tested, the content of chlorophyll a was strongly decreased, while the contents of chlorophyll b were slightly reduced without showing clear different among three groups and 15 cultivars. Carotenoid content was largely reduced by UV-B radiation, whereas polyamine content was moderately increased. The contents of MDA (malondialdehyde) that reflect the level of lipid peroxidation of cell membranes were clearly increased by UV-B stress, showing higher content in susceptible cultivars than moderate and torelant cultivars. The physiological important parameters highly related to visible injury were leaf color, chlorophyll, carotenoid, and lipid peroxidation, whereas biomass and polyamines were not closely correlated. Based on this results, it was concluded that changes of visible injury and the contents of chlorophyll and MDA could be adequately applied and utilized as physiological indicators to UV-B radiation.

Physiological and Ecological Studies( II) on Prolongation of the Green (한국잔디의 녹색기간연장을 위한 생리생태학적 연구(II))

  • 김형기
    • Asian Journal of Turfgrass Science
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    • v.4 no.1
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    • pp.5-11
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    • 1990
  • Korean Lawn. Zoysia japonica Steud. is the type of a perennial warm-season turfgrass keeping green leaf color for 5 months from May to September in natural condition in Korea. This Studies were carried out to obtain the effects of N-P-K fertilization, regrowth capacity after mowing and chlorophyll contents on prolongation of the green leaf color period in Zoysia japonica Steud turfgrass areas. The results obtained are summarized as follows ; 1.The effects of nitrogen fertilizer application in Zoysia japonica Steud, turfgrass areas were affected on regrowth ability after mowing green color of leaf canopies and chlorophyll contents, respectively(P<.05) 2.A experimental plots which applied 51 g/m$^2$/10 weeks of N$_3$P$_1$K$_1$fertilizer, there were high significant effects on prolongation of the green leaf color period and the plots of phosphorus and potassium had additive effects. 3.Prolongation of the green leaf color period to the last of October was obviously possible by slow degrees in proportation to increment of the fertilizer. However, it is interpreted that more prolongation of the green period is impossible with N-P-K fertilization and irrigation treatments. unless the intermediate turfgrasses between $C_3$ and $C_4$plants must be found and bred.

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Effects Of Active Okygen Species (^1O_2, O_2^-, H_2O_2$) and Scavengers on the Chlorophyll Bleaching of Leaf-Burning Disease from Panax ginseng C.A. Meyer (인삼엽요병에서 Active Oxygen Species (^1O_2, O_2^-, H_2O_2$)가 Chlorophyll Bleaching에 미치는 영향 및 방제대책에 관한 연구)

  • Yang, Deok-Cho;Kim, Myoung-Won;Chae, Quae;Kim, Myeong-Sik
    • Journal of Ginseng Research
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    • v.13 no.1
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    • pp.98-104
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    • 1989
  • In order to determine the specific active oxygen species directly related to chlorophyll bleaching in the leaf-burning disease, we investigated the effects of singlet oxygen (1O2), superoxide radical (O2-), and hydrogen Peroxide (H2O2) on isolated chloroplast suspension and leaf discs from Panax ginseng C.A. Meyer. When the singlet oxygen was added to the chloroplast suspension, the chlorophyll and carotenoid contents were decreased by more than 809), similar to treatment with high light intensity (100 KLux). We assumed that the conversion of dioxygen (O2) produced either in photolysis or in breakdown of hydrogen peroxide to singlet oxygen resulted from photorespiration. On the basis of these experiments , :he inhibitory effects of active oxygen scavengers propylgallic acid (PGA), 2,5-ditetrabutyl hydroquinon (DBH), sodium pyrosulfate (SPS), and ascorbic acid (ABS) were examined. In chloroplast suspension all four scavengers inhibited chlorophyll bleaching by more than 75fl , and in the leaf discs the inhibition rates of SPS, PGA and ABS were 46%, 51%, and 96% respectively.

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