• Title/Summary/Keyword: leaf and stem

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Study of the Function of β-D-fructofuranosidase by Plant Histological Method (식물 조직학적 방법에 의한 β-D-fructofuranosidase의 기능 연구)

  • Donggiun Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.4
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    • pp.587-592
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    • 2023
  • Plant growth is regulated by a variety of factors, including organic matter availability. Organic nutrients are carbohydrate molecules from photosynthetic products produced by tissues associated with carbon and energy fixation called "sources". These compounds flow through plant vascular bundles into non-photosynthetic or growing tissues called "sinks". Among these possible compounds, the disaccharide fructosyl glucose, sucrose, is the most representative. During the transport of sucrose, the pathway from the source to the sinks can include hydrolysis of sucrose into glucose and fructose derivatives or direct transfer of sucrose. Among the enzymes involved in this, β-D-fructofuranosidase is the most important. Soluble neutral β-D-fructofuranosidase, one of several isoenzymes, is located in intracellular protoplasts and helps plant cells metabolize sucrose to produce energy. In order to track the activity of this enzyme during the course of plant growth, histological methods were used for the most effective immunolocalization. As a result, the activity was higher in the phloem and epidermis than in the mesophyll tissue in the leaf. In the growing stem, activity was high in the phloem, epidermis, and cortex. The activity of the root, which is a sink tissue, was high in all parts, but especially the highest in the root tip part. It is thought that this is because it helps unloading of sucrose in sink tissues that require sucrose degradation and plays a role in hydrolysising sucrose.

Analysis of Biological Activities and Functional Components in Different Parts of Asparagus (아스파라거스 부위별 유효성분 및 생리활성 분석)

  • Kwon, Soon-Bae;Kwon, Hye-Jeong;Jeon, Shin-Jae;Seo, Hyun-Taek;Kim, Hee-Yeon;Lim, Jae-Gil;Park, Ji-Seon
    • Korean Journal of Food Science and Technology
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    • v.52 no.1
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    • pp.67-74
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    • 2020
  • In this study, various constituents and biological activities of different parts of asparagus were analyzed and compared. The Ca content was high in the leaves, K was significantly high in the top 25 cm of the spear, and Fe and Na were significantly high in the roots. The ascorbic acid, rutin, total polyphenol, and total flavonoid contents were high in the leaves, and the asparagine and glutathione contents were significantly high in the top 25 cm of the spear and roots, respectively. The bottom 5 cm of the spear had the highest saponin content compared with all other parts. The DPPH and ABTS radical scavenging activities were the highest in the leaves. The bottom 5 cm of the spear had the highest α-amylase inhibitory activity, whereas the stem showed the highest anti-inflammatory activity. These results suggest that the usually inedible parts of asparagus may be highly valuable as high-quality functional components owing to their antioxidative and anti-inflammatory activities.

The Effect of Soil Moisture Stress on the Growth of Barley and Grain Quality (토양수분 스트레스가 보리생육 및 종실품질에 미치는 영향)

  • Park, Moo-Eon
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.2
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    • pp.165-175
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    • 1995
  • To determine the effect of soil moisture stress on growth of barley and grain quality, a pot experiment was carried out for two barley varieties(Olbori and Chogangbori) by using large plastic pot(52cm in diameter and 55cm in depth) filled with sandy loam soil under rain-controlled open green house. By means of measuring soil water potential with micro tensiometer and gypsum block installed at 10cm in soil depth, soil moisture was controlled by sub-irrigation at several irigation points such as -0.05bar, -0.2bar, -0.5bar, -1.0bar, -5.0bar and -10.0bar in soil water potential. The lower soil water potential was controlled, the shorter length of stem and internode became, and the more narrow stem diameter was. Leaf area was significantly decreased when soil water potential was controlled lower than -0.5bar, although chlorophyll content of flag and first leaves was not changed so much. Weight of grain and ear was significantly decreased when soil water potential was lower than -5.0bar and the highest grain yield was obtaind in a plot where soil water potential was controlled at -0.2bar. However, the most efficient water use of Olbori and Chogangbori was obtained at -0.5bar and -1.0bar in water potentials, respectively. Crude protain content, maximum viscosity, consistency and ${\beta}$-glucan content of barley flour increased as soil water potential significantly decreased, especially below -5.0bar, but gelatination temperature decreased as soil water potential decreased.

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A Comparative Studies on the growth Characteristics and Feed Components of Sorghum × Sudangrass hybrids at Paddy Field Cultivation (수수×수단그라스 교잡종 논토양 재배시 품종별 생육특성 및 사료가치 비교 연구)

  • Jeon, Byong-Tae;Moon, Sang-Ho;Lee, Sang-Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.1
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    • pp.29-38
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    • 2012
  • This study was carried out to compare the agronomic characteristics, forage yield, and feed value of nine sorghum ${\times}$ sudangrass hybrids at paddy field cultivation. The nine recommended sorghum ${\times}$ sudangrass hybrids used in this study were P877F, Cow pow, Turbo gold, Maxi graze, Jumbo, G7, Sweet home, Honey chew BMR and Green star. Plant height, leaf number, stem diameter, and stem hardness were higher in Jumbo than other varieties (P<0.01). Sugar content (brix) was higher in order of P877F > Sweet home > maxi graze > Green star > Cow pow > G7 (P<0.01). Fresh (86,199kg/ha) and dry matter yield (16,206 kg/ha) of Jumbo were higher than other varieties (P<0.01). Crude protein was the highest in Maxi graze (6.5%), but crude fat was the highest in Honey chew BMR as 2.1% (P<0.01). NDF and ADF of Jumbo and G7 were higher than other varieties (P<0.01). TDN was higher in order of Maxi graze > Sweet home > P877F > Honey chew BMR > Green star, but no significant differences were found among the varieties. Minerals were the highest in Cow pow (15,020.5 mg/kg), and Sweet home (6,222.6 mg/kg) was the lowest as compared to other varieties (P<0.01). Total amino acids were higher in order of Maxi graze > Sweet home > Turbo gold > Honey chew BMR > Jumbo (P<0.01). Crude protein yield and crude fat yield were the highest in Turbo gold (814.6 kg/ha) and Honey chew BMR (309.8kg/ha), respectively (P<0.01). Mineral yield was the highest in Cow pow as 207.6 kg/ha (P<0.01). Amino acid yield (592.2 kg/ha) and TDN yield (10,194 kg/ha) were the highest in Sweet home. Based on the above results, sorghum ${\times}$ sudangrass hybrid varieties should be selected on the basis of the purpose of use. Because, each varieties showed various growth characteristics and nutrition yield (protein, fat, mineral, amino acid, TDN).

A Convergent Study on Applying a fine bubble to ginseng seedling cultivation (인삼 종묘삼 재배 시 파인버블을 적용하는 융합적 연구)

  • Ahn, Chul-Hyun
    • Journal of the Korea Convergence Society
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    • v.8 no.8
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    • pp.191-196
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    • 2017
  • This study was conducted to investigate the effect of fine bubble water treatment on the growth of ginseng seedlings. The growth of ginseng seedlings which were treated with general water and fine bubble water was investigated. The above ground part, the growth of leaf was increased by about 10% and the growth of the stem was about 6%, as the ginseng seedlings which were treated with fine bubble water comparing to the ginseng seedlings treated with general water. Root length was increased about 5%, root width was increased about 8%, roots weight was increased about 9%, and dry weight was increased about 7%. This is not because the whole root growth was increased, but the main root growth was increased about 7%. These results suggested that the physical properties of the fine bubble water stimulated the growth of ginseng seedlings. These results are initial study in the case of ginseng seedlings. Therefore, it can be applicable to the 3-5 years old ginseng plants. Further research will be needed to find out the optimal cultivation condition by controlling the dissolved oxygen amount according to the soil condition and the research applied to the ginseng seedlings.

A Study on the Land Improvement Effect of Sewage Sludge and Sewage Sludge Dry Fuel Mixture (하수슬러지와 하수슬러지 건조연료 혼합물의 토지개량효과에 관한 연구)

  • Kwon, Gi Woon;Park, Hye Ok;Lee, Kyeong Ho;Kim, Moon Jeong;Lee, Woo Weon;Ryu, Don Sik
    • Journal of the Korea Organic Resources Recycling Association
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    • v.28 no.3
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    • pp.45-49
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    • 2020
  • The aims of this study is to land improvement effect of sewage sludge dry fuel mixture. The mixing ratio of sewage sludge and dry fuel was mixed at a weight ratio of 1:1 to make a mixture, experimental designone was designed as one control (0 %) site and three test groups that each mixture of 10 %, 20 %, 30 % was added. Comparison of yield after cultivation of Kenaf 90days, in the 10 % test group the length of the stem increased by 73 % compared to control site and 20 % test group the leaf yield increased by 227 % compared to control site. It is judged that the growth rating the blooming of 30 % test group is faster than and the chlorophyll content is the highest(71.6SPAD) that of another control. In conclusion, the addition of dry fuel mixtures is effective in improving overall soil quality for plants to live.

Production of Antifungal Compost by Using Bacillus licheniformis KJ-9 (Bacillus licheniformis KJ-9를 이용한 항균발효퇴비의 생산)

  • Seo, Dong-Cheol;Ko, Jeong-Ae;Lee, Sang-Won
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1339-1344
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    • 2010
  • In order to produce environmental-friendly fermented compost, a cattle manure-sawdust compost (antifungal compost) was developed by inoculation of B. licheniformis KJ-9 to cattle manure-sawdust. The thermal stability of the antifungal substance produced by B. licheniformis KJ-9 maintained more than 60% antifungal activity with heat treatment at $100^{\circ}C$ for 10 min, and the optimum pH of antifungal activity of the substance was 7.0. In a pot experiment with red pepper, the antifungal compost increased 1.5~2 times in leaf number and stem and root growth rate compared to those of commercial compost. Also, the diameter of stems increased 1.5-3 times in the antifungal compost treated group. The amount of microbes increased markedly in soil supplemented with antifungal compost compared to the control. In the field experiment for cultivation of garlic and Perilla japonica, the growth of both crops was significantly enhanced in the field treated with antifungal compost as compared to the commercial compost.

Plant Regeneration and Multiplication of Gentiana scabra Bunge. through Leaf and Stem Culture (용담(Gentiana scabra Bunge.)의 엽육(葉肉) 및 줄기배양에 의한 식물체 재분화와 증식(增殖))

  • Seong, Nak-Sul;Park, Chung-Heon;Lee, Seoung-Tack;Kim, Seong-Min
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.2
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    • pp.129-136
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    • 1993
  • For the clonal proliferation of Gentiana scabra Bunge. which is one of the medicinaland ornamental plant, establishment multiplication of shoot through tissue culture technique and transplantation into soil were carried out. The shoot proliferation increased on the MS medium containing 0.5mg/l NAA and 0.5mg/l BAP. Optimum pH for shoot growth was pH 5.9, consequently MS medium supplemented with 2g/l activated charcoal was most effective for plant growth. There are two types of somaclonal variants, tall type was 63% and dwarf type was 37%.

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Immunolocalization of Wound-Inducible Insoluble Acid Invertases in Pea (Pisum sativum L) (완두콩(Pisum sativum L.) 상처에서 유도되는 불용성 산성 인버타제의 면역조직화)

  • Kim, Donggiun;Lee, Taek-Kyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.9
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    • pp.6425-6431
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    • 2015
  • Invertase, that hydrolyzes sucrose into glucose and fructose, plays a great role in carbohydrate reallocation between the photosynthetic source tissue and various sink tissues. Invertase also occurs in a variety of isoforms for various functions in plants. Insoluble invertases were extracted only in buffer solutions containing high concentrations of salt. Within these classes, acid invertase has an optimum activity at acidic pH (pH 4-5). Induction of insoluble acid invertase (INAC-INV) in leaf, stem, and root tissues in response to physical wounding has been investigated. To detect the localization of INAC-INV within the plant, immunolocalization has been performed. In this study, the accumulation of INAC-INV was noticeable to reach maximum levels on 72 hr after mechanical injuries. INAC-INV was induced in wounded leaves 3 times more than control leaves. Immunolocalization results showed that INAC-INV accumulated in wall appositions and intercellular spaces. INAC-INV was also localized at sieve cell walls in phloem tissues close to the site of wounding. Taken together, this study suggested that INAC-INV induction upon wounding injuries can play a role on responses to the high energy demand for wound healing process.

Utilization of Coconut Based Substrates for Nutriculture of Cut-chrysanthemum (절화 국화의 양액재배를 위한 코코넛 배지의 이용)

  • Jeong, Sung-Woo;Seok, Yong-Cheol;Bae, Eun-Ji;Kwon, Kee-Young;Huh, Moo-Ryong
    • Journal of agriculture & life science
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    • v.44 no.5
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    • pp.9-13
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    • 2010
  • This study was conducted to identify the possibility of utilization of coconut substrates for nutrition culture of cut-chrysanthemum. The materials of substrate were composed of dust, fiber, and chip from coco-nut fruit. Dust was used in dust (100%), and dust+chip and +fiber were used in the ratio of 7:3 (v:v), respectively, as coconut mixture substrate. Perlite was used as control in this experiment. Water content in the perlite medium was lower than in dust substrate. The pH of all coconut substrates ranged from 6.5 to 5.8, whereas perlite substrate ranged from 7.3 to 6.7. While, EC of dust substrates shown to be highest but perlite substrate was lowest. The growth of chrysanthemum such as stem length, leaf area, and dry matter showed better results in coconut substrates than that of perlite and dust. However, there was no differences days to in flowering among treatments.