• Mycobiology
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• v.48 no.6
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• pp.495-500
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• 2020

Leaf spot on lemon balm is frequently observed in Korea, causing considerable damage to crops. In 2014 and 2015, the occurrence of leaf spot was observed in several production greenhouses at Suwon, Gongju, and Namwon in Korea. Symptoms on lower leaves initially developed as small, distinct, discolored lesions, which enlarged progressively turning into dark brown, angular spots surrounded by purplish-brown margins. Based on the morphological characteristics and sequence analysis of actin (ACT), translation elongation factor 1-alpha (EF-1α), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II second largest subunit (RPB2), the fungus associated with the lemon balm leaf spot was determined as Septoria melissae. To the best of our knowledge, this is the first report of lemon balm leaf spot caused by S. melissae in Asia as well as in Korea.

• Journal of Mechanical Science and Technology
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• v.20 no.10
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• pp.1638-1645
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• 2006

This paper proposes a modeling technique which is able to not only reliably and easily represent the hysteretic characteristics but also analyze the dynamic stress of a taper leaf spring. The flexible multi-body dynamic model of the taper leaf spring is developed by interfacing the finite element model and computation model of the taper leaf spring. Rigid dummy parts are attached at the places where a finite element leaf model is in contact with an adjacent one in order to apply contact model. Friction is defined in the contact model to represent the hysteretic phenomenon of the taper leaf spring. The test of the taper leaf spring is conducted for the validation of the reliability of the flexible multi-body dynamic model of the taper leaf spring developed in this paper. The test is started at an unloaded state with the excitation amplitude of $1{\sim}2mm/sec$ and frequency of 132 mm. First, the simulation is conducted with the same condition as the test. Then, the simulations are conducted with various amplitudes in a loaded state. The hysteretic diagram from the test is compared with the ones from the simulation for the validation of the reliability of the model. The dynamic stress analysis of the taper leaf spring is also conducted with the developed flexible multi-body dynamic model under a dynamic loading condition.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.8
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• pp.1276-1290
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• 1997

The previous elastic stiffness formulas of leaf type holddown spring assemblies(HDSs) have been corrected and extended to be able to consider the point of taper runout for the TT-HDS and all the strain energies for both the TT-HDS and the TW-HDS based on Euler beam theory and Castigliano'stheorem. The elastic stiffness sensitivity of the leaf type holddown spring assemblies was analyzed using the derived elastic stiffness formulas and their gradient vectors obtained from the mid-point formula. As a result of the sensitivity analysis, the elastic stiffness sensitivity at each design variable is quantified and design variables having remarkable sensitivity are identified. Among the design variables, leaf thickness is identified as that of having the most remarkable sensitivity of the elastic stiffness. In addition, it was found that the sensitivity of the leaf type HDS's elastic stiffness is exponentially correlated to the leaf thickness.

• International Journal of Automotive Technology
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• v.6 no.6
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• pp.671-676
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• 2005

Spring is one of major suspension part of the light commercial vehicle (LCV). In the manufacturing process it is shot-peened to improve its fatigue strength. In this paper, residual stresses by shot peening were calculated through finite element analysis, and the effects of these residual stresses on fatigue strength of leaf spring were evaluated. Fatigue tests were performed with two kinds of specimens; one is actual leaf spring assembly, and the other is simulated 3-point bending specimen. Fatigue tests were performed under the loading condition that was measured on the proving ground. From the results, the maximum load-fatigue life relation of leaf spring was defined, and test results of 3 point bending specimen are in good agreement with those of leaf spring assembly. The effects of residual stresses by shot peening on fatigue strength of leaf spring is not large in the high load range, however, in the low load range, its effects were not negligible.

• Rapid Communication in Photoscience
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• v.3 no.2
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• pp.28-31
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• 2014

Loss of chlorophyll is the visible symptom of leaf senescence and staygreen refers to the delayed leaf senescence in plants. The staygreen gene (SGR) in rice (Oryza sativa L.) has been identified as its mutation maintains greenness during leaf senescence, and encodes a chloroplast protein required for the initiation of chlorophyll breakdown in plants. In this study, we isolated a rice SGR-homologous gene in creeping bentgrass (Agrostis stolonifera L.), and transgenic creeping bentgrass plants were obtained by introducing pCAMBIA3301 vector harboring antisense SGR gene under control of the senescence-specific SAG12 promoter. Transgenic plants were selected by herbicide resistance assays and genomic integration of the transgenes was confirmed by PCR analysis. Subsequent analyses demonstrated the staygreen phenotype of the transgenic creeping bentgrass plants with decreased chlorophyll loss during leaf senescence. These results suggest that the antisense SGR expression in creeping bentgrass delays leaf senescence, which provides a way to develop genetically engineered turfgrass varieties with the commercially useful staygreen trait.

• Research in Plant Disease
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• v.20 no.3
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• pp.219-222
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• 2014

Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.563-568
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• 1996

This experiment was conducted to obtain basic information of biochemical changes in the process of senescence by measuring the total RNA, protein, protease activity and electrophoretic pattern of protein in tobacco plant. The content of soluble protein increased by 15 days after leaf emergence and its level was not changed from 15 to 35 days after leaf emergence. The content of total RNA showed a maximum value at 15 days after leaf emergence and then decreased rapidly until 30 days after leaf emergence. The activity of protease of neutral fraction was higher than that of acidic fraction and rapidly increased up to the end of senescence after 50 days after leaf emergence. According to the analysis of electrophoresis, polypeptide band of 61kd was developed after 35 days after leaf emergence and increased by the end of senescence.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.111-115
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• 2006

This study was carried out to establish genetic transformation of Rot 3 gene into perilla plants and to evaluate aromatic compounds, brightness, anthocyanin contents and leaf index in Rot 3 overexpressing transgenic lines. Rot 3 transmitted successfully from T$_1$ to T$_2$ generation showing stable gene expression. It revealed that there was no difference between transgenic and non-transgenic plants in major agronomic characteristics of progeny analysis. There was not much difference in aromatic compounds and leaf brightness did not showed variations between transgenic and non-transgenic, but leaf index was distinguished, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.33 no.1
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• pp.21-27
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• 2011

Amadori compounds(1-deoxy-1-amino-2-ketoses) are important precursors of color, flavor and aroma produced in foods. Amadori compounds occur naturally in tobacco. The contribution of amadori compounds to smoke quality has been (of) interest because of their roles of the Maillard reaction in the leaf chemistry. The amounts of these compound in tobacco are affected by the processes of aging, drying and storage conditions. In this study, eight compounds were chemically synthesized because amadori compounds (have not been sold commercially these days.) were not available for obtaining commercially. The aim of this study was to develop the analytical method of amadori compounds in tobacco leaf by the liquid chromatography mass spectrometry using triple quadrupole analyzer(LC-MS/MS). This method was simple, rapid, selective and sensitive, and eight amadori compounds were simultaneously and quantitatively analyzed within 20 minutes. This method showed excellent accuracy and precision. Recovery rates of amadori compounds ranged from 86% to 102%, with relative standard deviation(RSD) ranged from 2.6% to 5.9%. This method was applied to analysis of amadori compounds contents of tobacco leaves in different varieties. Furthermore, it was expected that the method could be extended to the analysis of other amadori compounds.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.2
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• pp.133-143
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• 1992

The magnitude of additive effects generally parralled the magnitude of difference between parental means and appeared to be more independent from non - allelic interaction than dominance effects, whereas the magnitude of dominance effects were inflated by non-allelic interaction. Additive effects were significant for all characteristics observed and those of leaf width and leaf shape index of lugs, cutter and leaf and leaf width of tips were higher than dominance effects. Non-allelic gene interaction was expressed in leaf length of lugs, cutter and tips and heterosis of these characters were lower than the other characters.