• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.816-824
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• 2017

Human noroviruses are widespread and contagious viruses causing nonbacterial gastroenteritis. Real-time reverse transcription quantitative PCR (real-time RT-qPCR) is currently the gold standard for the sensitive and accurate detection of these pathogens and serves as a critical tool in outbreak prevention and control. Different surveillance teams, however, may use different assays, and variability in specimen conditions may lead to disagreement in results. Furthermore, the norovirus genome is highly variable and continuously evolving. These issues necessitate the re-examination of the real-time RT-qPCR's robustness in the context of accurate detection as well as the investigation of practical strategies to enhance assay performance. Four widely referenced real-time RT-qPCR assays (Assays A-D) were simultaneously performed to evaluate characteristics such as PCR efficiency, detection limit, and sensitivity and specificity with RT-PCR, and to assess the most accurate method for detecting norovirus genogroups I and II. Overall, Assay D was evaluated to be the most precise and accurate assay in this study. A ZEN internal quencher, which decreases nonspecific fluorescence during the PCR, was added to Assay D's probe, which further improved the assay performance. This study compared several detection assays for noroviruses, and an improvement strategy based on such comparisons provided useful characterizations of a highly optimized real-time RT-qPCR assay for norovirus detection.

• Journal of the Korean Society for Nondestructive Testing
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• v.24 no.5
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• pp.465-475
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• 2004

This paper is the first part of the study on the development of a smart active layer (SAL) sensor, which consists of two parts. In this first part, the theory and concept of the SAL sensor is investigated, which is designed for the detection of elastic waves caused by internal cracks and damages in structures. For the development SAL sensor, (i) the basic theory of elastic waves was studied, (ii) the feasible study of the SAL as an elastic waves detection sensor using the finite element analysis (FEA) with respect to a piezoceramic disc was performed. (iii) the comparison of performances between some piezoceramic sensors and a commercial acoustic emission (AE) sensor was accomplished to ensure the applicability by the experimental means, such as a pencil lead break test. Also, the conceptional study for the SAL sensor, which can be utilized for the effective detection and locating of defects by the arrangement of regularly distributed sensors, was discussed.

• Journal of Veterinary Clinics
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• v.8 no.1
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• pp.1-10
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• 1991

The B-lymphocyte differentiation from committed B-cell progenitors to antibody-secreting cells was discussed. B-cell progenitors derived from hematopoietic stem cells undergo the rearrangement of immunoglobulin(Ig) gene. The earliest cells as B-cell precursors have cytoplasmic Is(${\mu}$ chain). The entire Is molecule is expressed on the surface after synthesis of L chain. The resting B cells(Go stage) stimulated by binding antigen via Ig-receptors are activated(G$_1$ stage) and followed by proliferation(S stage), coupled with further selection(affinity maturation. class switch). The production of antibody against a particular antigen depends on the activation of B cells with surface Is capable of reacting with that antigen. This process does not occur in isolation but is controlled by helper and suppressor T cells and antigen presenting cells(APC). The mechanism of T cell-dependent B-cell response for production of antibody is largely explained by the cell to cell cooperation and soluble helper factors of T cells. 1) The antigen specific B cells and helper T cells are linked by Is-receptors, leading to the delivery of helper signals to the B cells. 2) Helper T cells recognize the processed antigen-derived peptides with the MHC class II molecules(la antigen) and is stimulated to secrete B-cell proliferation and differentiation factors which activate B cells of different antigenic specificity. The two models are shown currently 1) At low antigen concentration, only the antigen-specific B cell binds antigen and presents antigen-derived peptides with la molecules to helper T cells, which are stimulated to secrete cytokines(IL-4, IL-5, etc.) and 2) At high antigen concentration, antigen-derived peptides are presented by specific B cells, by B cells that endocytose the antigens, as well as by APC Cytokines secreted from helper T cells also lead to the activation of B cells and even bystander B cells in the on- vironmment and differentiate them into antibody-secreting plasma cells.

• Journal of the Korean Data and Information Science Society
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• v.21 no.1
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• pp.61-70
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• 2010

Today modern people have been searching on managing themselves in easy ways without side-effects to lead a healthy and quality life, accordingly concerns about complementary and alternative medicine have been increasing. The scientific term' complementary and alternative medicine' has been originally used in the US and has become the general term for natural treatment of medical methods, which can be suggested as an alternative solution to modern medical problems. There should be a study based on users' subjectivity to reinvigorate complementary and alternative medicine more than it does now. The purpose of this study is to offer basic materials when assessing practical applications on complementary and alternative medicine using the Q method which is subjective pattern analysis, help understand features of complementary and alternative therapy according to patterns, and find out preferences for patterns of complementary and alternative therapy. As a result patterns of complementary and alternative medicine were classified into three types. Firstly, type I prefers to biological/herbal treatment. Secondly, type II prefers to mixed treatments. Thirdly, type III prefers to manual healing.

• Applied Microscopy
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• v.20 no.1
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• pp.51-64
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• 1990

This experiment was performed to study the morphological changes of the goblet cells in the rabbit duodenal mucosa after common bile duct ligation. Healthy adult rabbits weighting about 2kg body weight were divided to normal and bile duct ligated groups. Common bile duct ligation was performed under ether anesthesia. Experimental animals were sacrificed on the 1st, 3rd, 5th, 7th and 14th day after the operation. Mucosal specimens from the upper part of duodenum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome, stained with uranyl acetate - lead citrate, and observed with JEM 100 CX II electron microscope. Observed results were as follow : 1. In the early stages(1st-5th day) of the experiments, the goblet cells showed apocrine and merocrine secretion. But those of the late stage(7th and 14th day) groups showed exocytotic merocrine secretion. 2. In the late stage of the experiments, there found than increase of newly formed goblet cells that contain electron lucent cytoplasms. 3. In the goblet cells of normal rabbit, mucous granules with higher or lower electron densities are found together in the cytoplasm, and electron lucent mucous granules occasionally fused together. But in the early stage of the common bile duct ligation, goblet cells contained granules of higher electron densities. 4. Considering the above findings, common bile duct ligation probably initiates the hypersecretion of mucous granules of goblet cells in the early stage, and may facilitate the differentiation of goblet cells in the later stage.

• Journal of Fashion Business
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• v.2 no.1
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• pp.25-34
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• 1998

This study has it's purpose in examining the materialized background. characteristic of African traditional costume and the A fro fashion of the year 1960. and it's influence on the contemporary fashion. Then the findings are applied to suggest a new way of image creation. 1960' s was the year in which people tried to free oneself from the ruling culture of the social standards. war. and the development of science. By such movement. people started to get interested in the environment and ecology. This then lead to the interest of the rights of the minority. With the youngs as the central figure A fro hair style and dashiki appeared as the street fashion. The characteristics of African costume applied to A fro fashion in 1990' s is as below. First. the North African style. Djellaba. and wrap style in the most common silhouette. Second. heavy materials such as stone. copper. silver. and gold are used. Necklace can be classified according to it's simple but. modern style. delicate but grand style. Bracelets are however. broad in width and many rings are worn widely. Third. Multi colored stripe and prints inspired by tattoo and deformation using red brown. dark beige. and orange are printed on textiles such as see through. Fourth. image of tattoo and deformation are applied to make-up. A fro hair and corn beads are also African taste. Fifth. African taste in recent fashion showed off the black beauty by appointing black models at the collection. In analyzing the study done above. characteristic images of African costume. accessaries and body painting was applied in presenting 3 creative designs. The first design named "Geometric I", took it's motif from the geometric pattern of the body painting with the afro hair. "Geometric II", the second design, showed it's application of geometric pattern of mutilation and the silhouette of the costume by using the see through. The third design called the "Geometric III" showed that the aesthetic and decorative side of clothing can be satisfied by applying various form and color of accessaries as the motif. A fro fashion is chosen as one of the folklore mood and it's beauty is conveyed on till today.

• Toxicological Research
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• v.11 no.1
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• pp.15-21
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• 1995

Several populations of lymphocytes possess receptors for autonomic neurotransmitter, which make lymphocytes susceptible to autonomic stimulation. This study was to evaluate the functional alternation of effector cells of the immune system. Female Balb/C mice, 15-20 g, were injected with MA subcutaneously under various conditions. Mixed lymphocyte reaction (MLR) showed certain T cell subsets were affected by MA. The level of interleukin-2 (IL-2) production was inhibited due to a defect in expression of the IL-2 receptor. In mice injected with 20 mg MA/kg, 1 day before assay, phagocytosis of peritoneal macrophages showed $14.07\pm3%$, which was similar degree to 5 mg MA/kg treatment for 4 consecutive days. Phagocytosis was almost recovered to that of control after 4 day in 20 mg/kg injected mice. Maximum inhibition of plaque forming cell (PFC) occurred when MA was given early, indicating the inductive time point of antibody production was affected. The cortisol level increased in the MA treated group (0.05, 0.20, and $0.08{\mu}g$/dl for control, low, and high dose-MA treated mice, respectively). Based on these results, MA has general suppression effects on the immune systems by functional alteration of effector cells. Considering the increment of serum cortisol levels, MA partially impacts the neuroendocrine system to lead to failure of immune response.

• The Journal of Information Technology
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• v.6 no.3
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• pp.151-168
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• 2003

The present paper attempts to describe on and off line integrated marketing and develop an empirical model for distribution industry. The focus is given on the small and medium size retail and service area. With both empirical case studies and literature review, we aim to develop empirical cases and propose a desirable marketing strategy for specific firms : BAROKOSA is pursues cooperation in wholesale business on the internet. For the purpose of the cooperative and integrated marketing of on and off line business, first of all, the firms should establish a clear target and share vision and mission for their directions. We suggest the following guidelines: (i) customer-based decision making, (ii) thoroughly cooperative competition, (iii) confidence in their work and business, (iv) courage to live up to action and (v) innovation to lead change.

• Parasites, Hosts and Diseases
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• v.22 no.1
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• pp.30-36
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• 1984

An ultrastructural study on the mature spermatoBoa oi Cloptorchis sineitsis was carried out. For this study, the liver cukes were collected from the livers of rabbits and rats artificially infected with the metacercariae obtained from the fresh water fish, Pseudorasbora parve. Six-month old worms were used. The collected liver fiukes were washed with 0.85% saline solution and then immediately moved to cold 2% glutaraldehyde buffered with 0.1M Millonig's phosphate buffier (pH 7.4) . The materials were dissected into appropriate pieces in the fixative about 30 minutes after beginning of the fixation. Two hours later the materials containing the seminal receptacle were rinsed several times with the buffier and were secondarily fixed with cold, bugeyed 1% osmium tetroxide(OsO4) for 2 hours. The fully mixed tissue blocks Ivere dehydrated in a series of graded concentrations of acetone and were embedded in Epon 812 mixture. Thin sections obtained from LKB-5 ultramicrotome were stained with uranyl acetate and Reynold's lead citrate. Observations of the sections were carried out with JEM-100CX II electron microscope, In general, the mature sperm was long thread-like form with a sickle-shaped head. According to the longitudinal sectioned view of the sperm tail, the nucleus seemed to be spirally coiled and run a little far along the tail. The acrosome was not observed. The cytoplasm of the tail was biflagellated as usual in trematodes. Unlike other platyhelminth spermatozoa, the sperm tail of Clenorchis sinensis showed the ${\ulcorner}9+2{\lrcorner}$ in the microtubular arrangement. The mitochondria with poorly developed cristae were observed throughout the middle piece. The middle piece of the tail showed dull ladder or triangular shapes with the two flagella at the bottom. But, the principal piece of the tail was slightly flattened cylindrical shape with two aagella within the cytoplasm. The end piece was uniflagellated. It was not clearly identised whether the end piece was subdivided into two by aagellum or the lengths of the two aagella were different. The glycogen granules were rich in the cytoplasm throughout the lenght of the spermatozoa. These granules might be the energy source for the movement of the spermatosoa.

• Journal of Animal Science and Technology
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• v.60 no.2
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• pp.3.1-3.9
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• 2018

Background: Poultry breeding has increased by 306% in Korea, inevitably increasing the production of manure which may contribute to environmental pollution. The nutrients (NP) in the manure are essential for crop cultivation and soil fertility when applied as compost. Excess nutrients from manure can be accumulated on the land and can lead to eutrophication. Therefore, a nutrient load on the finite land should be calculated. Methods: This study calculates the nutrient production from Korean poultry by investigating 11 broiler and 16 laying hen farms. The broiler manure was composted using deep litter composting while for layer deep litter composting, drying, and simple static pile were in practice. The effect of weight reduction and storing period during composting was checked. Three weight reduction cases of compost were constructed to calculate nutrient loading coefficients (NLCs) using data from; i) farm investigation, ii) theoretical P changes (${\Delta}P=0$), and iii) dry basis. Results: During farm investigation of broiler and layer with deep litter composting, there was a 68 and 21% N loss whereas 77 and 33% P loss was found, respectively. In case of layer composting, a loss of 10-56% N and a 52% P loss was observed. Drying manure increased the P concentrations therefore NLCs calculated using dry basis that showed quite higher reductions (67% N; 53% P). Nutrient loss from farm investigation was much higher than reported by Korean Ministry of Environment (ME). Conclusions: Nutrients in manure are decreased when undergo storing or composting process due to microbial action, drying, and leaching. The nutrient load applied to soil is less than the fresh manure, hence the livestock manure management and conservation of environment would be facilitated.