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Effects of Dietary Conjugated Linoleic Acid (CLA) and Oil Containing Unsaturated Fatty Acid Supplementation on Egg Production Rate and Quality in Laying Hens (산란계 사료내 Conjugated Linoleic Acid(CLA)와 불포화지방산 함유 Oil의 첨가가 산란율과 계란의 품질에 미치는 영향)

  • Kim, H.J.;Yoo, J.S.;Shin, S.O.;Cho, J.H.;Chen, Y.J.;Huang, Y.;Kim, Y.J.;Whang, K.Y.;Yang, M.S.;Kim, D.J.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.35 no.2
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    • pp.131-136
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    • 2008
  • This study was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) and oil containing unsaturated fatty acid supplementation on egg production and quality in laying hens. Two hundred-eighty eight, 36 week old ISA brown commercial layer, were employed in a 5 week feeding trial. Dietary treatments are 1) BO (basal diet + 1% soybean oil + 1% oat), 2) BS (basal diet + 1% safflower oil + 1% oat), 3) BF (basal diet + 1% free fatty acid + 1% oat), 4) CD (basal diet + 1% CLA containing diglyceride + 1% oat), 5) CT (basal diet + 1% CLA containing triglyceride + 1% oat) and 6) CP (basal diet + 1% CLA by-product + 1% soybean oil). For overall period, hen-day egg production was not significant among treatments (P>0.05). Egg shell breaking strength at 3 weeks in CP treatment was significantly lower than BS, BF, CD and CT treatments (P<0.05) and that at 4 weeks in BO and CP treatments was significantly lower than others (P<0.05). Egg shell thickness at 3 and 4 weeks in CP treatment was significantly lower than BS, BF, CD and CT treatments (P<0.05). Egg weight at 3 and 4 weeks in CP treatment was significantly lower than others (P<0.05). Yolk height at 4 weeks in BF and CT treatments was significantly highest compared to BO, CD and CP treatments (P<0.05) and among BO, CD and CP treatments, that in BO and CD treatments was significantly higher than CP treatment (P<0.05). At 4 weeks, yolk color in CP treatment was significantly higher than BO and BS treatments (P<0.05). Haugh unit at 3 and 4 weeks in BO and CP treatments was significantly lower than others (P<0.05). In conclusion, supplementing CLA and oil containing unsaturated fatty acid for laying hens improved egg shell breaking strength, egg shell thickness, egg weight, yolk height, yolk color and haugh unit.

Variations of Phytoplankton Standing Crops Affecting by Environmental Factors in the Marine Ranching Ground of Tongyeong Coastal Waters from 2000 to 2007 (2000$\sim$2007년 통영바다목장해역에서 환경요인의 영향에 따른 식물플랑크톤 현존량의 변화)

  • Jung, Seung-Won;Kwon, Oh-Youn;Joo, Hyoung-Min;Lee, Jin-Hwan
    • Korean Journal of Environmental Biology
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    • v.25 no.4
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    • pp.303-312
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    • 2007
  • In order to investigate the dynamics of phytoplankton standing crops affecting by environmental factors, biological and environmental factors, this study was examined in the marine ranching ground of Tongyeong coastal waters from 2000 to 2007. During the study, mean water temperature and salinity were 16.7$^{\circ}C$ and 32.9 psu, respectively. pH, DO and SS varied from 7.81$\sim$8.09, 3.02$\sim$8.97 mg $L^{-1}$ and 2.7$\sim$32.2 mg $L^{-1}$, respectively. Mean concentrations of dissolved inorganic nitrogen, phosphate and silicate were 21.75 ${\mu}M$, 0.90 ${\mu}M$ and 14.38 ${\mu}M$, respectively. Chlorophyll a concentrations varied from 0.02 ${\mu}g$ $L^{-1}$ to 25.29 ${\mu}g$ $L^{-1}$ with mean a value of 2.0 ${\mu}g$ $L^{-1}$. These factors did show significant differences on each layer and season, while did not show on the sampling stations. Phytoplankton standing crops varied from $4.21\times10^3$ cells $L^{-1}$ to $1.44\times10^6$ cells $L^{-1}$ with a mean value of $1.92\times10^5$ cells $L^{-1}$. Especially, variations of phytoplankton standing crops had an unimodal pattern as only bloomed in autumn rather than a bimodal pattern as generally bloomed in spring and autumn. In results of stepwise multiple regression analysis, the coefficient of determination $(R^2)$ for total standing crops was 0.35 and the standing crops were affected by water temperature, salinity, phosphate and silicate. The factors affected were different seasonally; water temperature in spring, salinity in summer, water temperature, salinity and silicate in autumn and water temperature, salinity and suspended solids in winter. Therefore, the results from the statistical analysis showed that the environmental factors influencing on the variations of the phytoplankton standing crops were predominantly water temperature and salinity.

Nutrition of Calcium and Phosphorus in Poultry Diets (닭에 대한 칼슘과 인의 영양)

  • 한인규;오상집
    • Korean Journal of Poultry Science
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    • v.8 no.2
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    • pp.55-68
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    • 1981
  • Calcium and phosphorus are not only indispensable for the bone formation and body fluids equilibrium but also are major components of egg shell. It is nutritionally important, therefore, to investigate the metabolism of calcium and phosphorus and to search for optimum requirement of calcium and phosphorus and the availability of various sources of calcium an4 phosphorus by poultry. An attempt was made to review the nutrition of calcium and phosphorus in poultry diets. 1, Calcium and phosphorus have great interrelationship with vitamin D in their metabolisms. 2. Most of the plant-origin phosphorus are existing in phytic form and it leads to low availability when used in poultry rations, although calcium and phosphorus present in animal-origin or mineral supplements are highly available in general. 3. Calcium and phosphorus requirement from existing information indicated that 1.0% calcium and 0.7% phosphorus for broiler and egg-type chicks, and 3.5% calcium and 0.4% phosphorus for laying hen. 4. It has been recommended that calcium and phosphorus level should be increased when the feed intake was decreased or when the egg Production rate was higher or when the hens are old. 5. Mono-, ci-, tri-, calcium phosphate, calcium carbonate, bone meal, limestone and oyster shell u the most readily available among various sources of calcium phosphorus supplements. Soft rock phosphate, deflourinated phosphate and gypsum are somewhat inferior to the previous ones in bioavailability. 6. The effect of particle size of calcium supplements on egg shell quality and egg production rate is not yet clearly defined but recent works showed that oyster shell is more available when it was coarse and limestone is more available when it was fine in panicle. size. 7. Present data indicated that mixed feeding of oyster shell and limestone is superior to the single feeding of each on laying performance. 8. Significant interaction between phosphorus and sodium was observed, that is, excessive sodium decreased egg production in layer and body weight growth in broiler in the low phosphorus diets but increased them in the high phosphorus diets.

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Study on Nucleation and Evolution Process of Ge Nano-islands on Si(001) Using Atomic Force Microscopy (AFM을 이용한 Si (001) 표면에 Ge 나노점의 형성과 성장과정에 관한 연구)

  • Park, J.S.;Lee, S.H.;Choia, M.S.;Song, D.S.;Leec, S.S.;Kwak, D.W.;Kim, D.H.;Yang, W.C.
    • Journal of the Korean Vacuum Society
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    • v.17 no.3
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    • pp.226-233
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    • 2008
  • The nucleation and evolution process of Ge nano-islands on Si(001) surfaces grown by chemical vapor deposition have been explored using atomic force microscopy (AFM). The Ge nano-islands are grown by exposing the substrates to a mixture of gasses GeH4 and H2 at pressure of 0.1-0.5Torr and temperatures of $600-650^{\circ}C$. The effect of growth conditions such as temperature, Ge thickness, annealing time on the shape, size, number density, and surface distribution was investigated. For Ge deposition greater than ${\sim}5$ monolayer (ML) with a growth rate of ${\sim}0.1ML/sec$ at $600^{\circ}C$, we observed island nucleation on the surface indicating the transition from strained layer to island structure. Further deposition of Ge led to shape transition from initial pyramid and hut to dome and superdome structure. The lateral average size of the islands increased from ${\sim}20nm$ to ${\sim}310nm$ while the number density decreased from $4{\times}10^{18}$ to $5{\times}10^8cm^{-2}$ during the shape transition process. In contrast, for the samples grown at a relatively higher temperature of $650^{\circ}C$ the morphology of the islands showed that the dome shape is dominant over the pyramid shape. The further deposition of Ge led to transition from the dome to the superdome shape. The evolution of shape, size, and surface distribution is related to energy minimization of the islands and surface diffusion of Ge adatoms. In particular, we found that the initially nucleated islands did not grow through long-range interaction between whole islands on the surface but via local interaction between the neighbor islands by investigation of the inter-islands distance.

Characteristics of a planar Bi-Sb multijunction thermal converter with Pt-heater (백금 히터가 내장된 평면형 Bi-Sb 다중접합 열전변환기의 특성)

  • Lee, H.C.;Kim, J.S.;Ham, S.H.;Lee, J.H.;Lee, J.H.;Park, S.I.;Kwon, S.W.
    • Journal of Sensor Science and Technology
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    • v.7 no.3
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    • pp.154-162
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    • 1998
  • A planar Bi-Sb multijunction thermal converter with high thermal sensitivity and small ac-dc transfer error has been fabricated by preparing the bifilar thin film Pt-heater and the hot junctions of thin film Bi-Sb thermopile on the $Si_{3}N_{4}/SiO_{2}/Si_{3}N_{4}$-diaphragm, which functions as a thermal isolation layer, and the cold junctions on the dielectric membrane supported with the Si-substrate, which acts as a heat sink, and its ac-dc transfer characteristics were investigated with the fast reversed dc method. The respective thermal sensitivities of the converter with single bifilar heater were about 10.1 mV/mW and 14.8 mV/mW in the air and vacuum, and those of the converter with dual bifilar heater were about 5.1 mV/mW and 7.6 mV/mW, and about 5.3 mV/mW and 7.8 mV/mW in the air and vacuum for the inputs of inside and outside heaters, indicating that the thermal sensitivities in the vacuum, where there is rarely thermal loss caused by gas, are higher than those in the air. The ac-dc voltage and current transfer difference ranges of the converter with single bifilar heater were about ${\pm}1.80\;ppm$ and ${\pm}0.58\;ppm$, and those of the converter with dual bifilar heater were about ${\pm}0.63\;ppm$ and ${\pm}0.25\;ppm$, and about ${\pm}0.53\;ppm$ and ${\pm}0.27\;ppm$, respectively, for the inputs of inside and outside heaters, in the frequency range below 10 kHz and in the air.

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A Study on the Water Quality Improvement in Semi-closed Sea Area Using Solar Powered Circulators (태양광 물 순환장치 가동에 의한 반폐쇄성 수역의 수질 변화)

  • Kim, Deok-Gil;Lee, Eun-Kyeong;Kim, Mu-Chan;Song, Sung-Kyu;Cho, Kwang-Soo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.15 no.3
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    • pp.257-262
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    • 2012
  • This study was conducted to verify the performance of the solar water circulation apparatus that was installed in a semi-closed sea area of Tongyeong to improve the water quality through removing thermocline and oxygen depleted water mass, and to prevent the occurrence of red tides caused by eutrophication. From 8 weeks of experiments, we found that the thermocline in the semi-closed sea area has been removed gradually after installation of the apparatus. The initial temperature of surface and bottom was $27.9^{\circ}C$ and $23.8^{\circ}C$, respectively and it was changed to $22.1^{\circ}C$ in both depth. In case of DO concentration, there was a big gap between surface (5.49 mg/L) and bottom (2.61 mg/L) and was an oxygen depleted water mass in the bottom area at initial. However DO concentration in bottom layer has increased gradually after operation (6.19 mg/L) and the oxygen depleted water mass has removed. Due to the effects of seasonal variation and the operation of the solar water circulation apparatus for 8 weeks, COD concentration decreased from 5.61 mg/L to 2.36 mg/L in surface area, and from 6.08 mg/L to 1.73 mg/L in bottom area. Dissolved inorganic nitrogen concentration also decreased from 0.135 mg/L to 0.050 mg/L in surface area, and from 0.076 mg/L to 0.051 mg/L in bottom area. This research was conducted from July to September, and it might be possible that the variation of water quality was affected by both seasonal variation and the operation of the water circulation apparatus. Hence a further research is required to verify the performance of the water circulation apparatus itself and to monitor dissolved inorganic nitrogen and phosphorous concentrations as well as Chl-a.

Amount and Chemical Characteristics of the Epicuticular Waxes on Leaves at Active Tillering and Heading Stages of Rice Varieties (벼 품종들의 분얼성기 및 출수기의 엽표면Wax의 양 및 화학적 조성)

  • Yong Woong, Kwon;Bong Jin, Chung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.2
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    • pp.185-197
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    • 1992
  • Differences in the amount and chemical characteristics of the epicuticular waxes on rice leaves were studied for the active tillering and heading stages of rice varieties differing widely in gross leaf-surface property and genetics. The amount of waxes on surfaces of rice leaf-blades was determined by extraction with chloroform and chemical composition of the waxes was characterized by thin layer chromatography, gas liquid chromatography and infrared spectrophotometry. The amount of waxes varied by variety and significantly with growth stage. The amount at the heading stage was 1.7 to 3.6 mg/g fresh weight of leaves, which was two to three times as much as that at the tillering stage of 0.8 to 1.8 mg/g fresh weight. The waxes consisted of seven chemical classes, namely diols, fatty acids, fatty alcohols, fatty aldehydes, fatty esters, saturated and unsaturated hydrocarbons. Diols and unsaturated hydrocarbons were identified as new chemical classes of the rice epicuticular waxes. The polar constituents such as dials, fatty acids and fatty alcohols and the non-polars such as fatty aldehydes, fatty esters, and saturated and unsaturated hydrocarbons were identified at the heading stage, but at the tillering stage only the non-polar compounds were identified. In the carbon numbers (C) of the chemical classes, diols were composed entirely of C30 and acids were mainly of C30 and C31. In alcohols, primary alcohols were composed of C13 and C32, and the secondary alcohols were of C14, C16 and / or C30 regardless of the rice varieties. The acid portion of fatty esters, mainly composed of C22 and C23, showed low cabon numbers compared with the aldehydes. The alcohol portion of them showed a wide distribution in carbon numbers from C13 to C26 depending on the rice varieties. Hydrocarbons had odd carbon numbers, consisting mainly of C29 and C31.

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THE EFFECTS OF DIETARY CONSISTENCY ON THE TRABECULAR BONE ARCHITECTURE IN GROWING MOUSE MANDIBULAR CONDYLE : A STUDY USING MICRO-CONFUTED TOMOGRAPHY (성장 중인 쥐에서 음식물의 경도가 하악 과두의 해면골에 미치는 영향 : 미세전산화 단층촬영을 이용한 연구)

  • Youn, Seok-Hee;Lee, Sang-Dae;Kim, Jung-Wook;Lee, Sang-Hoon;Hahn, Se-Hyun;Kim, Chong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.2
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    • pp.228-235
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    • 2004
  • The development and proliferation of the mandibular condyle can be altered by changes in the biomechanical environment of the temporomandibular joint. The biomechanical loads were varied by feeding diets of different consistencies. The purpose of the present study was to determine whether changes of masticatory forces by feeding a soft diet can alter the trabecular bone morphology of the growing mouse mandibular condyle, by means of micro-computed tomography. Thirty-six female, 21 days old, C57BL/6 mice were randomly divided into two groups. Mice in the hard-diet control group were fed standard hard rodent pellets for 8 weeks. The soft-diet group mice were given soft ground diets for 8 weeks and their lower incisors were shortened by cutting with a wire cutter twice a week to reduce incision. After 8 weeks all animals were killed after they were weighed. Following sacrifice, the right mandibular condyle was removed. High spatial resolution tomography was done with a Skyscan Micro-CT 1072. Cross-sections were scanned and three-dimensional images were reconstructed from 2D sections. Morphometric and nonmetric parameters such as bone volume(BV), bone surface(BS), total volume(TV), bone volume fraction(BV/TV), surface to volume ratio(BS/BV), trabecular thickness(Tb. Th.), structure model index(SMI) and degree of anisotropy(DA) were directly determined by means of the software package at the micro-CT system. From directly determined indices the trabecular number(Tb. N.) and trabecular separation(Tb. Sp.) were calculated according to parallel plate model of Parfitt et al.. After micro-tomographic imaging, the samples were decalcified, dehydrated, embedded and sectioned for histological observation. The results were as follow: 1. The bone volume fraction, trabecular thickness(Tb. Th.) and trabecular number(Tb. N.) were significantly decreased in the soft-diet group compared with that of the control group (p<0.05). 2. The trabecular separation(Tb. Sp.) was significantly increased in the soft-diet group(p<0.05). 3. There was no significant differences in the surface to volume ratio(BS/BV), structure model index(SMI) and degree of anisotropy(DA) between the soft-diet group and hard-diet control group (p>0.05). 4. Histological sections showed that the thickness of the proliferative layer and total cartilage thickness were significantly reduced in the soft-diet group.

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Initial results from spatially averaged coherency, frequency-wavenumber, and horizontal to vertical spectrum ratio microtremor survey methods for site hazard study at Launceston, Tasmania (Tasmania 의 Launceston 시의 위험 지역 분석을 위한 공간적 평균 일관성, 주파수-파수, 수평과 수직 스펙트럼의 비율을 이용한 상신 진동 탐사법의 일차적 결과)

  • Claprood, Maxime;Asten, Michael W.
    • Geophysics and Geophysical Exploration
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    • v.12 no.1
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    • pp.132-142
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    • 2009
  • The Tamar rift valley runs through the City of Launceston, Tasmania. Damage has occurred to city buildings due to earthquake activity in Bass Strait. The presence of the ancient valley, the Tamar valley, in-filled with soft sediments that vary rapidly in thickness from 0 to 250mover a few hundreds metres, is thought to induce a 2D resonance pattern, amplifying the surface motions over the valley and in Launceston. Spatially averaged coherency (SPAC), frequency-wavenumber (FK) and horizontal to vertical spectrum ratio (HVSR) microtremor survey methods are combined to identify and characterise site effects over the Tamar valley. Passive seismic array measurements acquired at seven selected sites were analysed with SPAC to estimate shear wave velocity (slowness) depth profiles. SPAC was then combined with HVSR to improve the resolution of these profiles in the sediments to an approximate depth of 125 m. Results show that sediments thicknesses vary significantly throughout Launceston. The top layer is composed of as much as 20m of very soft Quaternary alluvial sediments with a velocity from 50 m/s to 125 m/s. Shear-wave velocities in the deeper Tertiary sediment fill of the Tamar valley, with thicknesses from 0 to 250m vary from 400 m/s to 750 m/s. Results obtained using SPAC are presented at two selected sites (GUN and KPK) that agree well with dispersion curves interpreted with FK analysis. FK interpretation is, however, limited to a narrower range of frequencies than SPAC and seems to overestimate the shear wave velocity at lower frequencies. Observed HVSR are also compared with the results obtained by SPAC, assuming a layered earth model, and provide additional constraints on the shear wave slowness profiles at these sites. The combined SPAC and HVSR analysis confirms the hypothesis of a layered geology at the GUN site and indicates the presence of a 2D resonance pattern across the Tamar valley at the KPK site.

Establishment of a Single Dose Radiation Model of Oral Mucositis in Mice (일회 방사선조사를 이용한 마우스 구강점막염 모델의 확립)

  • Ryu, Seung-Hee;Moon, Soo-Young;Choi, Eun-Kyung;Kim, Jong-Hoon;Ahn, Seung-Do;Song, Si-Yeol;Park, Jin-Hong;Noh, Young-Ju;Lee, Sang-Wook
    • Radiation Oncology Journal
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    • v.26 no.4
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    • pp.257-262
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    • 2008
  • Purpose: Oral mucositis induced by radiotherapy to the head and neck area, is a common acute complication and is considered as the most severe symptom for cancer patients in the early stages of treatment. This study was proposed to establish the oral mucositis mouse model induced by a single dose of radiation for the facility of testing therapeutic candidates which can be used for the oral mucositis treatments. Materials and Methods: Fifty-five BALB/c mice were divided into four groups: control, 16 Gy, 18 Gy, and 20 Gy. Oral mucositis was induced by a single dose of radiation to the head and neck using 6 MV x-Ray from linear accelerator. After irradiation, body weight and physical abnormalities were checked daily. Tongue tissues from all groups were taken on days 1, 2, 3, 5, 7, 9, and 14, respectively and H&E staining was conducted to examine morphological changes. Results: Body weight dramatically decreased after day 5 in all irradiated mice. In the 16 Gy treatment group, body weight was recovered on day 14. The histology data showed that the thickness of the epithelial cell layer was decreased by the accumulated time after radiation treatment, up to day 9. Severe ulceration was revealed on day 9. Conclusion: A single dose of 16 Gy is sufficient dose to induce oral mucositis in Balb/C mice. Significant changes were observed in the Balb/C mice on days 7 and 9 after radiation. It is suggested that this mouse model might be a useful standard tool for studying oral mucositis induced by radiation.