• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.1
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• pp.28-37
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• 1996

Benzidine, an aromatic amine used primarily in the manufacture of azo dyes, is recognized as a urinary bladder carcinogen in humans. In rats, mice, and hamsters, chronic exposure to benzidine resulted in tumors of the liver. The present study was undertaken to suggest analyzing the metabolites of benzidine with the optimal condition, identify the metabolites of benzidine, and observe time variance of the metabolites in the isolated perfusated rat liver. N-acetylbenzidine was synthesized by acetylation of benzidine with acetic anhydride and separated by thin layer chromatography(TLC) and high performance liquid chromatography(HPLC). To analysis benzidine and the metabolites of benzidine, HPLC operating condition has been optimized by means of preliminary experiment. The mobile phase consisted of acetonitrile(37%) in phosphate buffer, flow rate maintained at 1.0 ml/min. Optimal detective conditions were electrochemicaldetector(ECD) at 0.75 V for benzidine and N-acetylbenzidine and ultravioletdetector(UVD) at 287 nm for N,N'-diacetylbenzidine. The separation system was composed of a guard column and a separation column(Polymer C18, $4.6{\times}250cm$) at a temparature of $40^{\circ}C$. The perfusion system was equilibrated for 30 minutes before addition of benzidine to the perfusate. Samples of the perfusate were collected at time intervals(0, 10, 20, 30, 60, 90, 120 min) during the 2 hour perfusion. Before analyzing samples by HPLC/ECD/UVD, samples had been treated with sep-pak. Samples of perfusate analyzed by HPLC/ECD/UVD and the metabolites of benzidine in the isolated perfused rat liver were N-acetylbenzidine and N,N'-diacetylbenzidine. Benzidine metabolized over 60% during the initial 30 minutes of perfusion, extensively by 1 hour, and was undetectable in the perfusate. N-acetylbenzidine increased by 30 minutes of perfusion, declined. N,N'-diacetylbenzidine increased the 0-90 minutes period, remained constant during the 90-120 minutes period.

• ALGAE
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• v.33 no.1
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• pp.1-19
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• 2018

Members of the family Parviluciferaceae (Alveolata, Perkinsozoa) are the well-known dinoflagellate parasitoids along with Amoebophrya ceratii species complex and parasitic chytrid Dinomyces arenysensis and contain six species across three genera (i.e., Parvilucifera infectans, P. sinerae, P. rostrata, and P. corolla, Dinovorax pyriformis, and Snorkelia prorocentri) so far. Among Parvilucifera species, the two species, P. infectans and P. sinerae, are very similar or almost identical each other morphologically and genetically, thereby make it difficult to distinguish between the two. The only main difference between the two species known so far is the number of sporangium wall (i.e., 2 layers in P. infectans vs. 3 layers in P. sinerae). During sampling in Masan bay, Korea during the spring season of 2015, the dinoflagellate Akashiwo sanguinea cells infected by the parasite Parvilucifera were observed and this host-parasite system was established in culture. Using this culture, its morphological and ultrastructural features with special emphasis on the variation in the number of sporangium wall over developmental times, were investigated. In addition, the sequences of rDNA regions and ${\beta}-tubulin$ genes were determined. The result clearly demonstrated that the trophocyte at 36 h was covered with 4 layers, and then outer layer of the sporocyte gradually degraded over time, resulting in wall structure consisting of two layers, with even processes being detached from 7-day-old sporangium with smooth surface, indicating that the difference in the number of layers seems not to be an appropriate ultrastructural character for distinguishing P. infectans and P. sinerae. While pairwise comparison of the large subunit rDNA sequences showed 100% identity among P. infectans / P. sinerae species complex, genetic differences were found in the small subunit (SSU) rDNA sequences but the differences were relatively small (11-13 nucleotides) compared with those (190-272 nucleotides) found among the rest of Parvilucifera species (P. rostrata and P. corolla). Those small differences in SSU rDNA sequences of P. infectans / P. sinerae species complex may reflect the variations within inter- strains of the same species from different geographical areas. Taken together, all morphological, ultrastructural, and molecular data from the present study suggest that they are the same species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.196-199
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• 2003

This experiment was conducted to evaluate the differences in physicochemical properties of soil, grain yield and milling recovery ratio and grain appearance of rice grown in long-term no-till and ordinary till systems. The paddy in no-till rice system was unploughed but straw-mulched for 15 years from 1988 to 2002 at the Experimental Paddy of Gyeongnam Agricultural Research and Extension Services, while the paddy in ordinary till system was ploughed and puddled every crop year, A 5cm organic layer was formed in 11-year no-till rice system, in which exchangeable cation and phosphate were accumulated. In no-till paddy organic matter, bulk density and solid phase of surface soil significantly increased, while pH, exchangeable cation, phosphate, liquid and gaseous phase decreased. Tillage made the very top soil soft, but made deep soil below 5cm significantly hard. In the 1st year of no-till, topsoil showed hard, but in the process of the time in no-till system, the top- and sub-soil showed softer, Yield and yield components of rice showed no differences between till- and no-till rice systems. Milling recovery ratio and grain appearance were not significantly different between no-till and till-rice systems.

• Journal of Conservation Science
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• v.33 no.6
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• pp.541-552
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• 2017

All excavated "buried cultural properties" are supervised by the Cultural Heritage Administration that decides whether to conserve them or not, depending on the necessity for conservation. Currently, there is no legal regulation concerning the administration of relics (including artifacts) and remains (including archaeological sites) that are relocated and restored based on such conservation decisions. Thus, administration of these cultural properties is inadequate. Under these circumstances, this paper recommends the current state of conservation and conservation methods for buried cultural properties excavated at Chungcheongnam-do thus far. Conservation of buried cultural properties excavated at Chungcheongnam-do primarily employ the "relocating and restoration" method; 23 relics and 46 remains employed relocation after dismantling and soil layer transcription method. Moreover, 27 remains were relocated after dismantling and 14 remains employed the transcription method. In terms of area, Buyeo-gun and Gongju-si had 12 relics accounting for about 50%, while according to the period, Baekjae period had 16 relics, accounting for about 70%. In terms of conservation facility, open-air facility had 33 relics, accounting for about 72%. Results of investigating the state of relics' management indicated that public institutions like a museum with a recognizable management system had their own work force and consequently ensured excellent conservation, whereas if there were no apparent responsible bodies, remains were subjected to considerable damage due to mismanagement. Therefore, this problem can be resolved by identifying a recognizable management body as well as ensuring periodical management and supervision. The excavated buried cultural properties are utilized not only for academic purposes, but also for PR or as exhibition material. Hence, this study confirms that meticulous management of buried cultural properties is currently required.

• The Journal of Engineering Geology
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• v.15 no.2 s.42
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• pp.105-121
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• 2005

Most of the landslides triggered by intensive rainfall on natural slope occur serious damages of human beings and properties. There have been many landslides since 1991 in Gyeonggi province. The influential factors are rainfall, topography, geology and soil properties. There were 660 landslides occurred by intensive rainfall as much as 250mm at Yongin and Anseong area from Tuly 19th to luly 21st,1991 This study surveyed location, topography, geology, geometry of the landslide and rainfall at the area and analyzed occurrence characteristics of the landslides. Most of the landslides occurred on the soil layer above the bed rock. They showed changes of landslide types from translational slides at the head part to flow at the central part. The landslides have relatively small magnitude of which length is shorter than 50m. In order to identify characteristics of landslides dependent on soil conditions, geotechnical properties of soil were evaluated by laboratory tests using soil samples which were collected on slided area and not-slided area. Soil properties of the slided area show high percentage of coarse material such as gravel and sand. However, soil of the not-slided area is mainly composed of fine material such as silt and clay. Permeability coefficients of the slided area are higher than those of the not-slided area.

• The Journal of Engineering Geology
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• v.20 no.2
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• pp.121-126
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• 2010

Current technology for radioactive waste disposal facility is operated as part of KURT site characterization in terms of reliability assessment is conducted to expand. In this study, a geological model of KURT surrounding area on the basis of flow characteristics of the site-scale hydrogeological study was about. Distributed in the study area into four boreholes were plotted using the stereo net NS, NW, EW, Low-angle fracture group was able to identify the components of geological models and include top soil layer, belt of weathering, Low-angle fracture zone, fracture zone was divided into. Separated by fracture of the hydraulic test of through the groundwater aquifer that provides the flow hydraulic conductivity and insulation hydraulic affecting the slope of the normal distribution for the size and direction by performing statistical analysis of fracture in the direction of local ns The advantage was confirmed. In addition, Low-angle fracture hydraulic conductivity of the value of 3.61e-07 m/s has a value greater than the major fracture, the fracture zones exist in the base rock and base rock and the hydraulic characteristics of the different methods applied and had to have a different interpretation judged by was.

• The Journal of Korean Society for Radiation Therapy
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• v.11 no.1
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• pp.16-21
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• 1999

Purpose : The aim of this study is to conform the possibility of the liquid type EPID as a QC tools to clinical indication and of replacement of the film dosimetry. Aditional aim is to describe a procedure for the use of a EPID as a physics calibration tool in the measurements of radiation beam parameters which are typically carried out with film. Method & Materials : In this study we used the Clinac 2100c/d with EPID. This system contains 65536 liquid-filled ion chambers arranged in a $256{\times}256$ matrix and the imaging area is $32.5{\times}32.5cm$ with liquid layer thickness of 1mm. The EPID was tested for different field sizes under typical clinical conditions and pixel values were calibrated against dose by producing images using various thickness of lead attenuators(lead step wedge) using 6 & 10MV x-ray. We placed various thickness of lead on the table of linear accelerator and set the portal vision an SDD of 100cm. To acquire portal image we change the field size and energy, and we recorded the average pixel value in a $3{\times}3$ pixel region of interest(ROI) at field center was recorded. The pixel values were also measured for different field sizes in order to evaluate the dependence of pixel value on x-ray energy spectrum and various scatter components. Result : The EPID, as a whole, was useful as a QA tool and dosimetry device. In mechanical check, cross-hair centering was well matched and the error was less than ?2mm and light/radiation field coincidence was less than 1mm also. In portal dosimetry the wider the field size the the higher the pixel value and as the lead thickness increase, the pixel value was exponentially decreased. Conclusions : The EPID was very suitable for QA tools and it can be used to measure exit dose during patients treatment with reasonable accuracy. But when indicate the EPID to clincal study deep consideration required

• Science of Emotion and Sensibility
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• v.13 no.2
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• pp.391-402
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• 2010

In this study, breathable waterproof materials were prepared by electrospinning. Five kinds of electrospun nanofiber web layered systems with different levels of nanofiber web density, as well as different substrates and layer structures were fabricated, and their mechanical properties (tensile, bending, shear, compression, surface, and thickness & weight) were measured by the KES-FB system and compared with those of conventional breathable waterproof fabrics (densely woven fabric, PTFE laminated fabric and PU coated fabric). The KES-FB measurements demonstrate that the lab-scale nanofiber web layered systems are more flexible and fuller than commercial nanofiber web layered systems, which have a more compact structure than the lab-scale nanofiber web layered systems. Densely woven fabrics and lab-scale nanofiber web layered systems showed lower values of tensile linearity (LT), bending stiffness (B), and shear stiffness (G) than those of PU coated and PTFE laminated fabric. These results indicate that they are more flexible and have less resistance to the shearing movement, corresponding to a more pliable material having a better drape, than PU coated fabrics and PTFE laminated fabrics.

• Korean Journal of Head & Neck Oncology
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• v.17 no.2
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• pp.155-161
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• 2001

Background and Objectives: Nitric oxide (NO) is generated in mammalian tissue by the conversion of L-arginine to L-citrulline. This reaction is catalyzed by nitric oxide synthase (NOS). NO is an important bioactive agent and a signalling molecule that mediates a variety of biologic actions such as vasodilation, neurotransmission, host defense, and iron metabolism but increased NO production may also contribute to the pathogenesis of a various of disorders, including cancer. Before now, the role of NO in thyroid gland is still investigated and it was supposed that NO mediate the angiogenesis in tumor growth. Others journal and works identified the expression of iNOS that involve by neutrophil and eNOS that involve in part in the vascular remodeling and to understand the role of NO in human thyroid gland. But authors revealed only eNOS in thyroid neoplasm. iNOS was identifed by inflammation in fault. Materials and Methods: Western blot analysis was performed, using a polyclonal antibody against eNOS (Rabbit polyclonal IgG). Using the same antibody, the distribution of eNOS was examined in 15 formalin-fixed paraffin embedded samples by immunohistochemistry. By NADPH consumption rate, NOS activity was estimated at nodular hyperplasia. Results: Western blot analysis exhibited that eNOS was significantly elevated in thyroid papillary carcinoma, compared to that in nodular hyperplasia and normal tissue. Immunohistochemistry showed that the immunoreacitivity was present more significantly in thyroid follicular epithelial cell layer than vascular endothelial cell. NOS activity increased in nodular hyperplasia. Conclusions: Thyroid papillary cancer without neutrophil invasion expressed only eNOS. The endothelial localization of eNOS may play an important role in pathogenensis of human thyroid nodular hyperplasia and the follicular localization of thyroid papillary carcinomas.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.10
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• pp.346-351
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• 2018

Metal three-dimensional (3D) printing technologies are mainly classified as powder bed fusion (PBF) and direct energy deposition (DED) methods according to the method of application of a laser beam to metallic powder. The DED method can be used to fabricate fine and hard 3D metallic structures by applying a strong laser beam to a thin layer of metallic powder. The PBF method involves slicing 3D graphics to be a certain height, laminating metal powders, and making a 3D structure using a laser. While the DED method has advantages such as laser cladding and metallic welding, it causes problems with low density when 3D shapes are created. The PBF method was introduced to address the structural density issues in the DED method and makes it easier to produce relatively dense 3D structures. In this paper, thin lines were produced by using PBF 3D printers with stainless-steel powder of roughly $30{\mu}m$ in diameter with a galvano scanner and fiber-transferred Nd:YAG laser beam. Experiments were carried out to find the optimal conditions for the width of a line depending on the processing times, laser power, spot size, and scan speed. The optimal conditions were two scanning processes in one line structure with a laser power of 30 W, spot size of $28.7{\mu}m$, and scan speed of 200 mm/s. With these conditions, a minimum width of about $85.3{\mu}m$ was obtained.