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Chemical Composition, Nutritional Value, and Saponin Content in the Spring Sap of Acer mono (고로쇠나무 수액(樹液)의 화학적(化學的) 성분(成分), 영양가치(營養價置)와 사포닌 함유(含有) 여부(與否)에 관(關)한 연구(硏究))

  • Lee, Kyung Joon;Park, Jong Young;Park, Kwan Hwa;Park, Hoon
    • Journal of Korean Society of Forest Science
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    • v.84 no.4
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    • pp.415-423
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    • 1995
  • This study was conducted to analyze the chemical composition, nutritional contents, and saponin in the xylem sap of Acer mono Max. From Feb 25 to Mar 4, 1994, spring sap was collected by making holes with 1.7cm diameter on the trunk of trees in Mt. Baekwoon and Mt. Jiri. Sugars were quantified by HPIC, amino acids by amino acid analyzer, saponins by TLC, HPLC, and $^1H$ & $^{13}C$ NMR. Major component of the sap was sucrose at a concentration range of 0.68 to 2.01%. Following minor components were found: glucose at 0.03-0.11%, and fructose at 0.01-0.03% as sugars, lipid at 0.03%, threonine at 0.152%, lysine at 0.038%, arginine at 0.068% as amino acids, ash at 0.1%, Ca at 175ppm, Fe at 2ppm, P at 19ppm, K at 16ppm, Na at 31ppm as minerals, vitamin $B_1$ at 0.6ppm, vit. $B_2$ at 0.1ppm, and vit. C at 19ppm. A trace of phenolic compounds was found by TLC, while saponin commonly reported in high-quality ginseng roots was not found in maple sap. It is concluded that sap of Acer mono contains a good variety of natural compounds such as sugars, amino acids, Ca, Fe, and vitamins to serve as an excellent source of very natural and health-promoting drink.

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A Study on the Amount and Chemical Compositions of Excreta from Laying Hens (산란계의 분뇨 배설량 및 이화학적 성상)

  • HwangBo, Jong;Hong, Eui-Chul;Na, Seung-Hwan;Yu, Dong-Jo;Kim, Hak-Kyu;Park, Mi-Na;Jung, Kie-Chul;Choo, Hyo-Jun;Park, Hee-Du;Seo, Ok-Suk
    • Korean Journal of Poultry Science
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    • v.36 no.4
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    • pp.337-342
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    • 2009
  • This work was conducted to investigate the manure excreta and chemical compositions of layers. Two hundred 1-d-old ISA Brown layers were used in this work, and ten of 200 layers were selected for measurement of manure excreta with feeding phase. Means of the manure excreta per bird, the moisture content of poultry, and the dried manure were $124.7\;{\pm}\;27.5\;g$/bird/day, $76.9\;{\pm}\;1.9%$, and $29.1\;{\pm}\;3.9\;g$/bird/day, respectively. N, $P_2O_5$, and $K_2O$ of manure (55 weeks) were $1.39\;{\pm}\;0.139$, $0.62\;{\pm}\;0.11$ and $0.68\;{\pm}\;0.09%$, respectively. pH, $BOD_5$, $COD_{Mn}$, and suspended solids (SS) were $8.19\;{\pm}\;0.71$, $50,266\;{\pm}\;621\;mg/L$, $62,832\;{\pm}\;803\;mg/L$, and $121,725\;{\pm}\;16,165\;mg/L$, respectively. Heavy metal contents in the manure were $0.003\;{\pm}\;0.0013\;ppm$ for Hg and $0.008\;{\pm}\;0.0012\;ppm$ for Pb, the latter of which was highly low compared with the standard (150 ppm), but no Cd was detected. The results of this work show the average excretion amounts and chemical compositions of layer's manure, which is used as an organic fertilizer.

Comparison of Carotenoid Pigments in Chinese muddy loach, Misgurnus mizolepis, and Muddy loach, Misgurnus anguillicaudatus, in the Subfamily Cobitidae (미꾸리아과에 속하는 미꾸라지와 미꾸리의 Carotenoid 색소성분의 비교)

  • PARK Eun-Sook;KANG Dong-Soo;HA Bong-Seuk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.3
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    • pp.265-271
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    • 1994
  • Differences in carotenoid composition in the integuments of wild and cultured chinese muddy loach Misgurnus mizolepis and muddy loach Misgurnus anguillicaudatus were compared. Total carotenoid contents in the integuments of the wild and cultured chinese muddy loach were $4.76mg\%\;and\;3.43mg\%$, respectively. The important carotenoids in the integuments of the wild chinese muddy loach were lutein($30.5\%$), ${\beta}$-cryptoxanthin($24.6\%$), ${\beta}$-carotene($20.6\%$) and cynthiaxanthin($11.7\%$). In addition, zeaxanthin($4.7\%$), tunaxanthin ($4.5\%$), and a-cryptoxanthin($1.0\%$) were present in small amounts. In the integuments of the cultured chinese muddy loach, lutein($35.4\%$), ${\beta}$-cryptoxanthin($17.9\%$), cynthiaxanthin($16.0\%$) and ${\beta}$-carotene($12.7\%$) were present as important carotenoids. In addition, zeaxanthin($8.1\%$), tunaxanthin($5.0\%$), a-cryptoxanthin($0.9\%$) were found in small amounts. Total carotenoid contents in the integuments of the wild and cultured muddy loach were $4.00mg\%\;and\;2.99mg\%$, respectively. The important carotenoids in the integuments of the wild muddy loach were lutein($32.9\%$), ${\beta}$-cryptoxanthin($18.8\%$), cynthiaxanthin($17.0\%$) and ${\beta}$-carotene($15.1\%$). In addition, zeaxanthin($6.5\%$), tunaxanthin($6.0\%$) and a-cryptoxanthin($1.5\%$) were found in small amounts. In the integuments of the cultured muddy loach, lutein($51.8\%$), cynthiaxanthin($19.9\%$) and ${\beta}$-cryptoxanthin($10.8\%$) were observed as important carotenoids. In addition, ${\beta}$-carotene($5.0\%$), zeaxanthin($4.8\%$), tunaxanthin($4.5\%$) and a-cryptoxanthin($0.2\%$) were found in small amounts.

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The KALION Automated Aerosol Type Classification and Mass Concentration Calculation Algorithm (한반도 에어로졸 라이다 네트워크(KALION)의 에어로졸 유형 구분 및 질량 농도 산출 알고리즘)

  • Yeo, Huidong;Kim, Sang-Woo;Lee, Chulkyu;Kim, Dukhyeon;Kim, Byung-Gon;Kim, Sewon;Nam, Hyoung-Gu;Noh, Young Min;Park, Soojin;Park, Chan Bong;Seo, Kwangsuk;Choi, Jin-Young;Lee, Myong-In;Lee, Eun hye
    • Korean Journal of Remote Sensing
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    • v.32 no.2
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    • pp.119-131
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    • 2016
  • Descriptions are provided of the automated aerosol-type classification and mass concentration calculation algorithm for real-time data processing and aerosol products in Korea Aerosol Lidar Observation Network (KALION, http://www.kalion.kr). The KALION algorithm provides aerosol-cloud classification and three aerosol types (clean continental, dust, and polluted continental/urban pollution aerosols). It also generates vertically resolved distributions of aerosol extinction coefficient and mass concentration. An extinction-to-backscatter ratio (lidar ratio) of 63.31 sr and aerosol mass extinction efficiency of $3.36m^2g^{-1}$ ($1.39m^2g^{-1}$ for dust), determined from co-located sky radiometer and $PM_{10}$ mass concentration measurements in Seoul from June 2006 to December 2015, are deployed in the algorithm. To assess the robustness of the algorithm, we investigate the pollution and dust events in Seoul on 28-30 March, 2015. The aerosol-type identification, especially for dust particles, is agreed with the official Asian dust report by Korean Meteorological Administration. The lidar-derived mass concentrations also well match with $PM_{10}$ mass concentrations. Mean bias difference between $PM_{10}$ and lidar-derived mass concentrations estimated from June 2006 to December 2015 in Seoul is about $3{\mu}g\;m^{-3}$. Lidar ratio and aerosol mass extinction efficiency for each aerosol types will be developed and implemented into the KALION algorithm. More products, such as ice and water-droplet cloud discrimination, cloud base height, and boundary layer height will be produced by the KALION algorithm.

Preparation of Nanoporous Activated Carbon with Sulfuric Acid Lignin and Its Application as a Biosorbent (황산 가수분해 잔사 리그닌을 이용한 나노 세공 활성탄 제조 및 친환경 흡착제로의 활용 가능성 평가)

  • Hwang, Hyewon;Choi, Joon Weon
    • Journal of the Korean Wood Science and Technology
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    • v.46 no.1
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    • pp.17-28
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    • 2018
  • In this study, catalytic activation using sulfuric acid lignin (SAL), the condensed solid by-product from saccharification process, with potassium hydroxide at $750^{\circ}C$ for 1 h in order to investigate its potential to nanoporous carbon In this study, catalytic activation using sulfuric acid lignin (SAL), the condensed solid by-product from saccharification process, with potassium hydroxide at $750^{\circ}C$ for 1 h in order to investigate its potential to nanoporous carbon material. Comparison study was also conducted by production of activated carbon from coconut shell (CCNS), Pinus, and Avicel, and each activated carbon was characterized by chemical composition, Raman spectroscopy, SEM analysis, and BET analysis. The amount of solid residue after thermogravimetric analysis of biomass samples at the final temperature of $750^{\circ}C$ was SAL > CCNS > Pinus > Avicel, which was the same as the order of activated carbon yields after catalytic activation. Specifically, SAL-derived activated carbon showed the highest value of carbon content (91.0%) and $I_d/I_g$ peak ratio (4.2), indicating that amorphous large aromatic structure layer was formed with high carbon fixation. In addition, the largest changes was observed in SAL with the maximum BET specific surface area and pore volume of $2341m^2/g$ and $1.270cm^3/g$, respectively. Furthermore, the adsorption test for three kinds of organic pollutants (phenol, 2,4-Dichlorophenoxyacetic acid, and carbofuran) were conducted, and an excellent adsorption capacity more than 90 mg/g for all activated carbon was determined using 100 ppm of the standard solution. Therefore, SAL, a condensed structure, can be used not only as a nanoporous carbon material with high specific surface area but also as a biosorbent applied to a carbon filter for remediation of organic pollutants in future.

THE STUDY ON THE MICROLEAKAGE PATTERN OF FLOWABLE COMPOSITE RESIN RESTORATIONS ACCORDING TO THE TYPE OF ADHESIVE MATERIALS (접착제에 따른 유동성 복합 레진 수복물의 미세누출 양상에 관한 연구)

  • Park, Ji-Eun;Kim, Jong-Soo;Yoo, Seung-Hoon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.3
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    • pp.456-468
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    • 2008
  • This study was performed to evaluate the quality of newly offered dentin bonding system($AdheSE^{(R)}$ One) by comparing the degree of microleakage measured with those of several conventional adhesive materials(AQ Bond Plus and $Adper^{TM}$ Single Bond 2). The quality of hybrid layer and resin tags was analyzed by observing restoration/ tooth interface under SEM. All-in-one system is in the limelight for having advantage of reducing chair time of children with difficult behavior pattern. Therefore the possibility of clinical application of All-in-one system was evaluated. The results obtained are as follows; 1. At the enamel margin, group II(AQ Bond Plus) showed the highest value of microleakage, and the other groups showed decreased value in order of group III($AdheSE^{(R)}$ One) and I($Adper^{TM}$ Single Bond 2). There was statistically significant difference between group II and the others(p<0.05), and no statistical difference was found between group I and III. 2. At the dentin margin, microleakage value was increased in order of group II, I, III and significant difference between all groups(p<0.05). 3. In group I and III, microleakage value measured at the enamel margin was significantly lower than that seen at the dentin margin(p<0.05), and there was no statistical difference in group II. 4. Resin tags observed under SEM were very weak and tangled in group II and III while the strong and thick tags were observed in group I. In conclusion, careful case selection and accurate clinical application is recommended in using AQ Bond Plus and $AdheSE^{(R)}$ One, giving consideration of the results showing its higher microleakage and weaker strength than $Adper^{TM}$ Single Bond 2.

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Lymphocyte Subpopulations and Proliferation of T cells, Phagocytic Activity of Leukocytes on Alcoholics (알코올중독자의 백혈구탐식능, 림프구아형 및 증식능)

  • 김용호;서병배;이정녀;김영훈
    • Biomedical Science Letters
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    • v.2 no.2
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    • pp.167-174
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    • 1996
  • Alcoholics increased susceptibility to microbial infection that is associated with decreased immunity. but there has been little experimental evidence to support alcoholics-induced increase of microbial infection directly in non-specific immunity. Therefore, we were used the method of phagocytic-plaque including all the stimulating factors for the phagocytosis, subtypes of lymphocytes and T-lymphocyte proliferation. The experimental groups were divided into 3 groups: (1) alcoholics who were hospitalized less than 1 week (newly hospitalized alcoholics), (2) alcoholics who were hospitalized more than 2 weeks (old hospitalized alcoholics), (3) healthy blood donors. We have studied 98 alcoholics and 35 healthy blood donors and control groups. A physician has checked the biological markers and diagnosed the body-condition alcoholics. The immunity and non-specific immunity on the alcoholics were analyzed by using the simultest kit and flow cytometry. Proliferation of the lymphocytes was analyzed by the phytohemmagglutinine mitogen. Phagocytosis and migration properties of leukocytes were identified on the layer formed by Staphylococcus aureus Cowan I strain. Biological markers of alcoholics and control groups, by such as blood glucose, ${\gamma}$-glutamyl transpeptidase and mean corpuscular volumes of red blood cells, were determined by biochemical and hematological methods. Compared with control groups, cluster of differentiation (CD)3+, CD8+ and CD19+ in alcoholic were more decreased except CD4+/CD8+ ratio. Proliferation of the T-lymphocytes, phagocytosis and migration properties of the leukocytes in alcoholics were decreased compared with those of control groups. According to the results observed in our experiment, they can be summerized as follows: 1, Cellular, humoral and non-specific immunities, are markedly decreased in alcoholics than those in control groups. 2. It is inferred that Phagocytic plaque formation is a very useful method to evaluate phagocytosis and migration properties of the alcoholic leukocytes 3. It is thought that the subtypes of lymphocytes, especially CD4+/CD8+ ratio, are essential methods to analyzed the alcoholic immunity. 4. Specific and non-specific immunity on the old hospitalized alcoholics was slightly increased, which depends upon the alcoholic medication.

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Particle-size Effect of Silicate Fertilizer on Its Solubility and Mobility in Soil (토양(土壤)에 처리한 광재규산질비료의 입도별(粒度別) 용해도(溶解度) 및 이동성(移動性))

  • Yoo, Sun-Ho;Park, Lee-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.13 no.2
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    • pp.57-63
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    • 1980
  • The effect of particle size of silicate fertilizer, crushed slag from the steel industry, on the behavior of silicate in soil was investigated through laboratory experiments. The silicate fertilizer was sieved to obtain three fractions of particles, coarser than 10 mesh 20-35 mesh, and finer than 100 mesh. Silicate concentration of the extract obtained by shaking 20 mg of particles, coarser than 10 mesh, 20-35 mesh, and finer than 100 mesh, in 50 ml of distilled water for 4 hours was 0.3, 1.0, and 3.2 ppm respectively. As shaking the mixture of the silicate fertilizer and soil proceeded, silicate concentration of the extract increased, and this increase after 4 hour shaking was attributed mainly to dissolution of soil silicate. When the mixture of soil and the silicate fertilizer was incubated under submerged condition, silicate concentration of the solution decreased for the first 2-4 weeks, thereafter increased with incubation time. During this incubation period, silicate concentration of the solution changed inversely with pH of the solution. After 6-10 weeks, however, both silicate concentration and pH of the solution increased with incubation time. Silicate concentration of the effluent from the 14.5 cm soil column of which top 4.5 cm was packed with the mixture of 30 g of soil and 30 mg of the silicate fertilizer reached maximum at 0.94 pore volumes for the particles of 20-35 mesh and 1.03 pore volumes for the particles finer than 100 mesh, whereas the effluent concentration reached maximum at 0.88 pore volumes for the soil column without the silicate fertilizer treatment. Soil analysis made after water percolation revealed that 1.5 pore volumes of water could leach down large amount of the water soluble silicate but not the sodium acetate extractable silicate, from top 3-6 cm soil layer.

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Ultra-Structures And $^{14}C$-Mannitol Transport Study of Human Nasal Epithelial Cells Using ALI Culture Technique (ALI 배양법 이용한 비강 점막 상피세포의 미세구조와 $^{14}C$-mannitol 투과도)

  • Kwak, Kyung-Rok;Hwang, Jee-Yoon;Lee, Ji-Seok;Park, Hye-Kyung;Kim, Yun-Seong;Lee, Min-Ki;Park, Soon-Kew;Kim, Yoo-Sun;Roh, Hwan-Jung
    • Tuberculosis and Respiratory Diseases
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    • v.50 no.2
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    • pp.205-212
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    • 2001
  • Background : The information on nasal transport and the metabolism of peptides have been obtained from pharmacokinetic investigations in experimental animals. However, there are no transport and metabolic studies of human nasal epithelial cells. In this study, the permeability characteristics and the metabolic properties of in vitro human nasal cell monolayers were investigated. Material and Methods : Normal human inferior nasal conchal tissue samples were obtained from patients undergoing endoscopic nasal cavitary surgery. The specimens were cultured in a transwell using an air-liquid Interface (ALI) culture, and the transepithelial electrical resistance (TEER) value of the blank filter and confluent cell monolayers were measured. To determine the % leakage of mannitol, $4{\mu}mol%$ $^{14}C$-labelled mannitol was added and the % leakage was measured every 10 minute for 1 hour. Result : Human nasal epithelial cells in the primary culture grew to a confluent monolayer within 7 days and expressed microvilli. The tight junction between the cells was confirmed by transmission electron microscopy. The TEER value of the blank filter, fifth day and seventh day reached $108.5\;ohm.cm^2$, $141\;ohm.cm^2$ and $177.5\;ohm.cm^2$, respectively. Transcellular % leakage of the $^{14}$-mannitol at 10, 20, 30, 40, 50 and 60 minutes was $35.67{\pm}5.43$, $34.42{\pm}5.60$, $32.75{\pm}5.71$, $31.76{\pm}4.22$, $30.96{\pm}3.49$ and $29.60{\pm}3.68\;%$, respectively. Conclusion : The human nasal epithelial monolayer using ALI culture techniques is suitable for a transcellular permeability study. The data suggests that human nasal epithelial cells In an ALI culture technique shows some promise for a nasal transport and metabolism study.

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Theoretical Analysis of Bragg-Reflector Type FBAR with Resonance Mode (공진 모드에 따른 Bragg-Reflector Type FBAR 의 이론적 분석)

  • 조문기;윤영섭
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.40 no.11
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    • pp.9-18
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    • 2003
  • Two configurations of Film Bulk Acoustic Wave Resonators with acoustic quater-wave bragg reflector layers are theoretically analyzed using equivalent circuits and the difference of their characteristics are discussed. We compare the characteristics of λ/2 mode to those of ideal FBAR with top and bottom electrode contacting air and the characteristics of λ/4 mode to those of ideal FBAR with top electrode contacting air and bottom electrode clamped. We assume that the piezoelectric film is ZnO, the electrode is A1 and the substrate is Si, ABCD parameters are extracted and input impedance is calculated by converting the equivalent circuit from Mason equivalent circuits to the simplified equivalent circuits that ABCD parameters are extracted possible, From the variation of resonance frequency due to the change of thickness of reflector layers and the variation of electrical Q due to the change of mechanical Q of reflector layers, it is confirmed that the reflector layer just under the bottom electrode have the greatest effect on the varation of resonance frequency and electrical Q. It is shown that the number of reflector layers required for the saturation of electrical Q decreases with the increase of the impedance ratio of reflector layers and electrical Q of λ/2 mode is larger than that of λ/4 mode, Electromechanical coupling factor is independent of the number of layers, The impedance ratio of reflector layers becomes larger as the electromechanical coupling factor becomes larger, The electromechanical coupling factor of the two mode are smaller than those of ideal FBARs because of the trapping of acoustic energy in the reflector layers, The insertion loss of the ladder filter decreases with the increase of the number of reflector layers but the bandwidth is not affected much by the number of reflector layers, As the impedance ratio of reflector layers becomes larger the insertion loss becomes smaller and the bandwidth becomes wider, In our analysis of the two mode, characteristics of λ/2 mode appear to be slightly more favorable than that of λ/4 mode