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Isolation of marine algicidal bacteria from surface seawater and sediment samples associated with harmful algal blooms in Korea (유해조류번성 주변의 해수와 침전물에서 살조균의 분리)

  • Kristyanto, Sylvia;Kim, Jaisoo
    • Korean Journal of Microbiology
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    • v.52 no.1
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    • pp.40-48
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    • 2016
  • This study mainly focused on isolation of marine algicidal bacteria associated with phytoplankton blooms and characterization of algicidal activity against harmful algae. Harmful algal blooms (HABs) found naturally in surface waters have caused many environmental problems worldwide. In this study, forty bacterial strains that have capability of inhibiting harmful algal growth were isolated from Masan Bay, Jinhae Bay, Dol Island, Jangmok Bay, and the Tongyeong Sea, Republic of Korea. The bacteria were screened furthermore for the characteristics on algicidal activities against Cochlodinium polykrikoides, Chattonella marina, Skeletonema costatum, Heterosigma akashiwo, Heterocapsa triquetra, Prorocentrum minimum, and Scrippsiella trochoidea. As a result, the algicidal bacteria that were screened from double over layer agar and microscopic counts tests belonged to genera Pseudomonas, Vibrio, Bacillus, Pseudoalteromonas, Ruegeria, Joostella, Marinomonas, Stakelama, Porphyrobacter, and Albirhodobacter. One of the most important HAB species is Co. polykrikoides and the strongest algicidal activity against the dinoflagellate was 94.00% after 6 h treatment with 10% bacterial culture filtrate. In this study, Marinomonas sp. M Jin 1-8, Stakelama sp. ZB Yeonmyeong 1-11 & 1-13, Porphyrobacter sp. M Yeonmyeong 2-22, and Albirhodobacter sp. 6-R Jin 6-1 were found to be as new genera of bacteria having anti-algal activity. These results suggest that these bacteria might play an important role in controlling phytoplankton blooms.

Na3PO4 Flame Retardant Treatment on Lyocell Fiber for Thermal Stability and Anti-oxidation Properties (라이오셀의 열 안정 및 내산화 특성 향상을 위한 Na3PO4 내염화 처리)

  • Kim, Hyeong Gi;Kim, Eun Ae;Lee, Young-Seak;In, Se Jin
    • Fire Science and Engineering
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    • v.29 no.2
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    • pp.25-32
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    • 2015
  • The improved thermal stability and anti-oxidation properties of lyocell fiber were studied based on flame retardant treatment by using $Na_3PO_4$ solution. The optimized conditions of flame retardant treatment were studied on various concentrations of $Na_3PO_4$ and the mechanism was proposed through experimental results of thermal stability and anti-oxidation. The integral procedural decomposition temperature (IPDT), limiting oxygen index (LOI) and activation energy ($E_a$) increased 30, 160% respectively via flame retardant treatment. It is noted that thermal stability and anti-oxidation improved based on char and carbon layer formation by dehydrogenation and dissociation of C-C bond resulting the hindrance of oxygen and heat energy into polymer resin. The optimized conditions for efficient flame retardant property of lyocell fiber were provided using $Na_3PO_4$ solution and the mechanism was also studied based on experimental results such as initial decomposition temperature (IDT), IPDT, LOI and $E_a$.

Effects of Spent Layer Crushed Breast Addition on the Quality of the Cremi during Storage (노계 분쇄 가슴육의 첨가가 크래미의 저장 중 품질변화에 미치는 영향)

  • Jin, Sang-Keun;Park, Jae-Hong;Shin, Daekeun
    • Food Science of Animal Resources
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    • v.33 no.2
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    • pp.251-257
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    • 2013
  • To evaluate the effects of surimi substitution to spent laying hen crushed breast meat on the quality of the Cremi during storage, this study was conducted. Spent laying hen breast meat was crushed using a silent cutter, which is normally adapted to the sausage process. The Cremis were manufactured by the addition of 5 or 10% CSBM and separated to three different groups including control and 5 (05CM) and 10% crushed meat (10CM) treatments. All cremis were packaged and stored at $10{\pm}1^{\circ}C$ for 3 weeks, and the analysis for the determination of physicochemical and sensory properties of cremi was conducted on week 0 and 3 of storage only. Moisture content was significantly differed depending on storage days and both 05CM and 10CM had high moisture content as compared to that of control (p<0.05). In addition, TBARS was lowered when surimi was substituted up to 10% CM as compared to cremis manufactured by the addition of 5% CM. The cremis sampled from 05CM and 10CM treatments had low in CIE $a^*$, in contrast showed high in CIE $b^*$ than control (p<0.05). The substitution of surimi to CM did not influence on sensory characteristics even though shear force and gel characteristics were affected (p<0.05). In conclusion, the results indicate that although significantly lowered shear force and gel characteristics were determined in 05CM cremis (p<0.05), trained sensory panels did not recognize the differences of 5 or 10% CM addition. Therefore, it seems that the substitution of surimi up to 10% CM may be secured and economically considerable.

A Study of Damage Assessment Caused by Hydrogen Gas Leak in Tube Trailer Storage Facilities (수소 Tube Trailer 저장시설에서의 수소가스 누출에 따른 사고피해예측에 관한 연구)

  • Kim, Jong-Rak;Hwang, Seong-Min;Yoon, Myong-O
    • Fire Science and Engineering
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    • v.25 no.6
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    • pp.32-38
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    • 2011
  • As the using rate of an explosive gas has been increased in the industrial site, the regional residents adjacent to the site as well as the site workers have frequently fallen into a dangerous situation. Damage caused by accident in the process using hydrogen gas is not confined only to the relevant process, but also is linked to a large scale of fire or explosion and it bring about heavy casualties. Therefore, personnel in charge should investigate the kinds and causes of the accident, forecast the scale of damage and also, shall establish and manage safety countermeasures. We, in Anti-Calamity Research Center, forecasted the scope of danger if break out a fire or/and explosion in hydrogen gas facilities of MLCC firing process. We selected piping leak accident, which is the most frequent accident case based on an actual analysis of accident data occurred. We select and apply piping leak accident which is the most frequent case based on an actual accident data as a model of damage forecasting scenario caused by accident. A jet fire breaks out if hydrogen gas leaks through pipe size of 10 mm ${\Phi}$ under pressure of 120 bar, and in case of $4kw/m^2$ of radiation level, the radiation heat can produce an effect on up to distance of maximum 12.45 meter. Herein, we are going to recommend safety security and countermeasures for improvement through forecasting of accident damages.

Preparation and Properties of Chlorine-Resistance Loose Reverse Osmosis Hollow-fiber Membrane (내염소성 중공사형 역삼투막(Loose RO)의 제조 및 특성)

  • Kim, Se-Jong;Woo, Seung-Moon;Hwang, Hae-Yong;Koh, Hyung-Chul;Ha, Seong-Yong;Choi, Ho-Sang;Nam, Sang-Yong
    • Membrane Journal
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    • v.20 no.4
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    • pp.304-311
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    • 2010
  • In this study, loose RO hollow fiber membranes using CTA polymer were prepared by phase inversion method and their water purification properties were tested. 1,4-dioxane and LiCl was used as a skin layer formation agent and pore formation agent, respectively. Water flux, salt rejection, chlorine resistance, MWCO and membrane morphology were evaluated as a function of the dope composition. When the membrane prepared using the dope solution of CTA/NMP/1,4-dioxane = 18/72/10 (wt%) with air gap of 30 cm, it shows improved RO performance such as $20.5L/m^2hr$ of water flux, 60% of NaCl rejection, 10,000 ppm/hr of chlorine-resistance and around 5,000 Da of MWCO.

A Method to Find Feature Set for Detecting Various Denial Service Attacks in Power Grid (전력망에서의 다양한 서비스 거부 공격 탐지 위한 특징 선택 방법)

  • Lee, DongHwi;Kim, Young-Dae;Park, Woo-Bin;Kim, Joon-Seok;Kang, Seung-Ho
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.2
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    • pp.311-316
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    • 2016
  • Network intrusion detection system based on machine learning method such as artificial neural network is quite dependent on the selected features in terms of accuracy and efficiency. Nevertheless, choosing the optimal combination of features, which guarantees accuracy and efficienty, from generally used many features to detect network intrusion requires extensive computing resources. In this paper, we deal with a optimal feature selection problem to determine 6 denial service attacks and normal usage provided by NSL-KDD data. We propose a optimal feature selection algorithm. Proposed algorithm is based on the multi-start local search algorithm, one of representative meta-heuristic algorithm for solving optimization problem. In order to evaluate the performance of our proposed algorithm, comparison with a case of all 41 features used against NSL-KDD data is conducted. In addtion, comparisons between 3 well-known machine learning methods (multi-layer perceptron., Bayes classifier, and Support vector machine) are performed to find a machine learning method which shows the best performance combined with the proposed feature selection method.

Sensitivity Analysis of the High-Resolution WISE-WRF Model with the Use of Surface Roughness Length in Seoul Metropolitan Areas (서울지역의 고해상도 WISE-WRF 모델의 지표면 거칠기 길이 개선에 따른 민감도 분석)

  • Jee, Joon-Bum;Jang, Min;Yi, Chaeyeon;Zo, Il-Sung;Kim, Bu-Yo;Park, Moon-Soo;Choi, Young-Jean
    • Atmosphere
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    • v.26 no.1
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    • pp.111-126
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    • 2016
  • In the numerical weather model, surface properties can be defined by various parameters such as terrain height, landuse, surface albedo, soil moisture, surface emissivity, roughness length and so on. And these parameters need to be improved in the Seoul metropolitan area that established high-rise and complex buildings by urbanization at a recent time. The surface roughness length map is developed from digital elevation model (DEM) and it is implemented to the high-resolution numerical weather (WISE-WRF) model. Simulated results from WISE-WRF model are analyzed the relationship between meteorological variables to changes in the surface roughness length. Friction speed and wind speed are improved with various surface roughness in urban, these variables affected to temperature and relative humidity and hence the surface roughness length will affect to the precipitation and Planetary Boundary Layer (PBL) height. When surface variables by the WISE-WRF model are validated with Automatic Weather System (AWS) observations, NEW experiment is able to simulate more accurate than ORG experiment in temperature and wind speed. Especially, wind speed is overestimated over $2.5m\;s^{-1}$ on some AWS stations in Seoul and surrounding area but it improved with positive correlation and Root Mean Square Error (RMSE) below $2.5m\;s^{-1}$ in whole area. There are close relationship between surface roughness length and wind speed, and the change of surface variables lead to the change of location and duration of precipitation. As a result, the accuracy of WISE-WRF model is improved with the new surface roughness length retrieved from DEM, and its surface roughness length is important role in the high-resolution WISE-WRF model. By the way, the result in this study need various validation from retrieved the surface roughness length to numerical weather model simulations with observation data.

A study for conservation of plant-based cultural properties : on the subject of straw sandals excavated at Goongnamji in Buyeo (초본류 문화재의 보존처리를 위한 연구 -부여 궁남지 출토 짚신을 대상으로-)

  • Na, Mi-Sun;Kim, Ik-Joo;Kim, Soo-Ki
    • 한국문화재보존과학회:학술대회논문집
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    • 2004.10a
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    • pp.115-130
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    • 2004
  • Plant-based cultural assets using straw and grass as household goods of our people's have been used as indispensable tools for practical living for a very long time. However, only a limited number of artifacts were unearthed so far due to tile fragility of the material. For this reason, research on plant-based cultural properties had close to no progress, and the appropriateness of the PEG method, high-grade alcohol method, alcohol--ether-resin method, and Paraloid B-72 used in preserving plant-based cultural properties has not been sufficiently investigated. Therefore, this study examined the weight change rate by applying the methods of Primal MC-76 and vacuum freeze-drying used mostly as a earth-layer hardening material among PEG and acrylic resin, which are applied widely for preservation of waterlogged archaeological wood, as a means to preserve plant-based cultural properties along with the examination of the subject material, and an experiment was also performed on moisture absorption. The findings as a result were, first, the plant-based material being studied was found to be Typha (Typha orientalis Presl). Secondly, the weight change experiment applying $PEG\#400$ and $PEG\#4000$ confirmed a steady increase of weight if PEG -2Step is used for treatment. Third, in preserving all subject materials with soil, treatment with $PEG\#4000$, Primal MC-76, and vacuum freeze-drying showed that tile vacuum freeze-drying method resulted in the largest or $20\%$ reduction in weight, while Primal MC-76 resulted in $18\%$ and $PEG\#4000$ in $8\%$ of weight reduction. It was concluded that, considering the stability of soil measurement, this came to be because resin permeation was carried out along with tile drying process. Fourth, the weight changes were found to be around $10\%$ in various humidity conditions after the preservation treatment. The greatest weight change rate was seen in the case of $PEG\#4000$, particularly having chemicals gush out in a high humidity (RH $84\%$ or higher) environment. In the case of Primal MC-76 and vacuum freeze-drying methods, $6\~8\%$ weight changes were detected, and the lowest weight change was found in the case of the vacuum freeze-drying method. Fifth, as for color changes after treatment, blackening occurred most strongly with $PEG\#4000$, while Primal MC-76 and vacuum freeze-drying manifested colors closest to dry straw or grass. However, the texture of straw was not very evident in the case of Primal MC-76, due to a glossy surface, but vacuum freeze-drying was found to offer tile best result in terms of texture. Putting together the results of the above experiments, vacuum freeze-drying presented after being treated with PEG2-Step the most stabilized changes in weight, while it offered the smallest change in color as well.

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A Study on the Manufacturing Technique by Scientific Analysis and Reproduction Experiment of Ancient Silver Objects Excavated from Neungnae-ri, Ganghwa Island (강화도 능내리출토 은제유물의 과학적 분석 및 재현실험을 통한 제작기법 연구)

  • Ryu, Dong-Wan;Kim, Soo-Ki
    • Journal of Conservation Science
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    • v.27 no.1
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    • pp.1-11
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    • 2011
  • For the silver artifacts in the Koryo Dynasty excavated from Neungnae-ri Ganghwa island, the metallographic section analysis and hardness and chemical analysis were conducted. After making samples in the similar ratio of the composition concentration, the changes of the microstructure were checked according to the working method and temperature. The results show that those silver artifacts are Au-Cu alloys with 2 to 6 % of Cu. From the results it is judged that Cu was artificially alloyed with them to keep the proper hardness and identified that they were gilded by the amalgamation process seeing that mercury was included at the guilt layer. Also the porous texture on the surface of them could be formed at over $400^{\circ}C$, therefore, it is assumed the hot working or heat treatment at over $400^{\circ}C$ were performed. In silver artifacts made by the relief and repousse, they have the similar composition analysis to other 7 artifacts but the hardness is lower than pure silver. Consequently from differences in the hardness, it can be inferred that the low hardness of silver artifacts is concerned with manufacturing techniques.

Archaeological Scientific Characteristics of Patternless Pottery with Talc Temper: Baekseokdong Gojaemigol Site in Cheonan, Korea (활석비짐 무문토기의 고고과학적 특성: 천안 백석동 고재미골 유적)

  • Kim, Su Kyoung;Lee, Chan Hee
    • Journal of Conservation Science
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    • v.31 no.2
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    • pp.159-173
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    • 2015
  • The patternless potteries excavated from the Baekseokdong Gojaemigol site in Cheonan, was subdivided into talc and non-talc (including amphibole) type pottery. The potteries showed black, reddish brown and yellowish brown colors, but represent to difference with occurrences and internal texture of raw materials and temper minerals. The all potteries and paleosoils are commonly high content of temper minerals with poorly sorting and roundness of particles, and the paleosoils composed mainly of quartz, plagioclase, mica, chlorite and kaolinite. Between the talc and non-talc type potteries are very similar with magnetic susceptibility, absorption ratio and specific gravity. Geochemical behaviors of major, minor, compatible and incompatible elements in talc pottery are very similar with amphibole, non-talc pottery and paleosoils, and well correspondence with enrichment and deficiency patterns of each element, and the talc and amphibole potteries are highly enriched patterns of MgO concentration. In paleosoils of Gojaemigol site, talc and amphibole are not detected, therefore, making the pottery of the site estimate the possible to artificial additions of the temper minerals of talc and amphibole used interbedded talc layer within gneiss complex near the Baekseokdong area. Based on the phase relations, differential thermal and thermal gravimetric analyses, the potteries could be classified into two groups by firing temperature. The one group of talc temper pottery fired from 800 to $870^{\circ}C$ and the other group of amphibole and non-talc temper pottery revealed of 900 to $950^{\circ}C$.