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Second Look Arthroscopic Finding after Fibrin Matrix Autologous Chondrocyte Implantation for the Treatment of Articular Cartilage Defect of the Knee - Preliminary Report - (슬관절 연골 결손에 대한 fibrin matrix 자가 연골 세포 이식술 후 이차 관절경 소견 - 예비보고 -)

  • Choi, Sung-Wook;Oh, In-Suk;Kim, Ryuh-Sup;Park, Sun-Won;Lee, Jong-Min;Lee, Moon;Kim, Myung-Ku
    • Journal of the Korean Arthroscopy Society
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    • v.11 no.1
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    • pp.1-6
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    • 2007
  • Purpose: The purpose of this preliminary report is to investigate the short term outcome of performing gel type fibrin matrix autologous chondrocyte implantation to patients who have damaged knee joint cartilage using secondary arthroscopy. Material and Methods: Six patients who have damaged knee joint cartilage were involved. The average size of defect was $5.13\;cm^2$. While performing primary arthroscopy, whole layer of cartilage bone was obtained either from the margin of damaged cartilage or the bilateral margin of a trochlea. The cartilaginous cells were obtained for culture for four to six weeks. While performing secondary minimal invasive arthrotomy, gel type fibrin matrix autologous chondrocyte was implanted on the chondral defect site. Results: 4 among 6 patients to be more than good in Modified Cincinnati Knee Scoring system. Lysholm function score was 59.5 preoperatively, and it improved to 76.25. ICRS grading by performing secondary arthroscopy revealed 4 out of 6 patients to be nearly normal. Conclusion: Gel type fibrin matrix autologous chondrocyte implantation is a treatment for cartilage defect, which takes less time to operate than the conventional implantation. In addition, this method minimizes the size of incision and allows arthroscopic surgery. However, long term follow up and more case study is thought to be necessary.

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Correlations between the Fatty Acid Composition of Serum Phospholipids and Blood Pressure

  • Lee, Yang-Cha-Kim;Shim, Yoo-Jin;Chung, Eun-Jung;Kim, Soo-Yeon;Yangsoo Jang;Lee, Jong-Ho
    • Nutritional Sciences
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    • v.5 no.2
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    • pp.68-74
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    • 2002
  • Studies of the relationship between the composition of serum fatty acids and blood pressure are complex and controversial. Fatty acids, important constituents of biological membranes, could potentially affect vasoreactivities including blood pressure. In this study the compositions of fatty acids in serum phospholipids were compared between three types of hypertensive subjects (men, pre-menopausal women, and post-menopausal women) and their respective nrmotensive controls. Serum lipids were extracted and phospholipids were separated by thin layer chromatography. The percentage of palmitic acid (16 : 0) in serum phospholipids was significantly higher and the percentage of stearic acid (18 : 0) was significantly lower in all three hypertensive groups, compared with their corresponding control groups. Only in the group of post-menopausal women, palmitic acid was closely associated wish increases in both systolic (SBP) and diastolic blood pressure (DBP), while stearic acid was associated with decreases in both SBP and DBP. The polyunsaturated fatty acids in serum phospholipids behaved differently from saturated fatty acids. The ratios of products / precursor fatty acids, such as $\sumLCPUFA\omega6/18 : 2\omega$6, 20 : 4$\omega$6/18 : 2$\omega$6, ∑LCPUFA$\omega$3/18 : 3$\omega$3 and 22 : 6$\omega$3/20 : 5$\omega$3, were all clearly associated with both SBP and DBP in hypertensive, post-menopausal women. Desaturation and elongation in fatty acid metabolism could affect the bioavailability of eicosanoid precursors. Changes in the constituent fatty acids of phospholipids and eicosanoid precursors may also influence fluidity, ionic transport, hormone receptors and enzyme activities in biological membranes. In conclusion, both systolic and diastolic blood pressure in post-menopausal women was positively associated with the level of palmitic acid, and negatively associated with the level of stearic acid, in serum phospholipids. The relationships between serum phospholipid-$\omega$6 and $\omega$3 series fatty acids and blood pressure in women, especially in post-menopausal women, require further investigation by taking into consideration hormonal status and eicosanoid metabolism. Funker study is needed to determine the value of dietary manipulation of fatty acid constituents of serum phospholipids, relating to hypertension in women.

Numerical Simulation of Flow Characteristics behind a Circular Patch of Vegetation using a Two-Dimensional Numerical Model (2차원 수치모형을 이용한 원형군락 하류의 흐름특성 수치모의)

  • Kim, Hyung Suk;Park, Moonhyeong
    • Journal of Korea Water Resources Association
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    • v.48 no.11
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    • pp.891-903
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    • 2015
  • This paper presents numerical simulations of flow around a circular patch of vegetation using a depth-averaged two-dimensional numerical model which is capable of simulating flow structure in vegetated open channel. In order to account for vegetation effect, drag force terms are included in governing equations. Numerical simulations are conducted with various solid volume fractions (SVF). Flow passes through a circular patch and low velocity region, which is called wake region, is formed downstream of the patch. When SVF is larger than 0.08, a recirculation is observed. The location of the recirculation is moved further downstream as SVF decreases. Von-$K{\acute{a}}rm{\acute{a}}n$ vortex street is developed beyond the wake region due to interaction between two shear layers induced by a circular patch of vegetation. The vortex is developed as SVF is larger than 0.08, and the location of the vortex is consistent with the maximum of turbulence kinetic energy. The location of the peak of turbulence kinetic energy is moved further downstream as SVF decreases.

A Study on the Variation of Air Temperature in the Box Containing Dry Ice for Transportation of the Pine Mushroom, Tricholoma matsutake (Ito et Imai) Sing. (드라이아이스를 넣은 송용상자내(松茸箱子內)의 기온변화(氣溫變化)에 관(關)한 연구(硏究))

  • Lee, Ji-Yul;Kim, Hyung-Bae
    • The Korean Journal of Mycology
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    • v.3 no.2
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    • pp.19-23
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    • 1975
  • A study on the variation of the air temperature in the pine mushroom box containing dry ice for the preservation of the freshness of the pine mushroom, Tricholoma matsutake (Ito et Imai) Sing. produced in Korea was made in Seoul, Korea during the period of August 1 to 30, 1975. A safe shipment of 5 kg (net weight) of fresh mushrooms with freshness preserved for 24 hours in August required the placement of 4.5 kg of dry ice-a half in the upper portion and the other half in the middle portion-in an airtight container (mushroom box). The container used for the shipment test, made of 3mm-thick double-layer cardboard, was of the dimensions : $0.33{\times}0.4{\times}0.5=0.066(m^3)$. As the temperature fell, decreased amounts of dry ice were needed : 3 to 2 kg in September and 1.5 to 0.3 kg in October. With this preservation method, large quantities of fresh mushrooms were exported to Tokyo ana Osaka, Japan without damage in the process of transportation.

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Analysis of the Factors Influencing the Mesopore Ratio on the Soil Surface to Investigate the Site Factors in a Forest Stand(I) - With a Special Reference to Coniferous Stands - (산림(山林)의 입지환경인자(立地環境因子)가 표층토양(表層土壤)의 조공극률(粗孔極律)에 미치는 영향인자(影響因子) 분석(分析)(I) - 침엽수림(針葉樹林)을 중심(中心)으로 -)

  • Jeong, Yongho;Park, Jae Hyeon;Kim, Kyong Ha;Youn, Ho Joong
    • Journal of Korean Society of Forest Science
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    • v.90 no.3
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    • pp.314-323
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    • 2001
  • This study aimed to clarify the influencing factors of mesopore ratio on a pore geometry of surface soil in coniferous stands as an index of the water retention capacity. Twenty three factors including site conditions and soil properties were analyzed by spss/pc + for the data collected during March to October of 1993. The factors influencing the mesopore ratio(pF2.7) on the surface soil were as follows; macropore ratio(pF1.6), slope, crown-cover rates, thickness of F layer, organic matter contents, and the growing stock. And influencing factor on the ratio of mesopore in the soil surface was correlated with percentage of amount of clay, soil surface, A and B horizon soil hardness shows high negative significance. Also, multiple regression equations for mesopore ratios of surface soil and surface soil hardness, clear length, growing stock, B horizon of soil hardness, organic matter contents show high significance($R^2$; 0.80). In coniferous stands, it is effective in promoting development on the ratio of mesopore that forest practice for enhancing of the water resource retention capacity should be carried out when the crown-cover rates of stands are more than 80 percentages.

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Chemical Treatment of Fouling Organisms on Fishing Nets (어망에 부착하는 오손생물의 화학처리 방법)

  • HUR Sung-Bum;CHUN Seh-Kyu;KIM Hyung-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.241-248
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    • 1986
  • New Paint, Stapol N-6200D, products from Song Woun Industrial Co. Ltd., was tested on its chemical effect on fouling organisms attached to fishing nets. Two kinds of fishing nets with different mesh size, 5 mm (cage culture net) and 33 mm (set net) were painted and attached to $1m^2$ PVC quadrates. The quadrates with painted or non-painted fishing nets were placed at the surface water layer of southern coast of Korea. Monthly species composition and abundance of fouling organisms were analysed as well as total weight of fouling organisms including mud on fishing nets. On the other hand, anti-fouling effect of the organisms and mud on different color paints was studied and the durability of chemical effect of painted fishing net on fouling organisms was also estimated.

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Voltammetric Determination of Dopamine with the N-Hydroxysuccinimide Modified Carbon Paste Electrode (N-히드록시숙신이미드로 수식한 탄소반죽전극을 이용한 도파민의 전기화학적 측정)

  • Yoo Jae Hyun;Yoo Byung Wook;Kim Soon Shin;Uhm Jung Hee;Nam Hakhyun;Cha Geun Sig
    • Journal of the Korean Electrochemical Society
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    • v.4 no.3
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    • pp.109-112
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    • 2001
  • An activated carbon paste electrode was modified with the N-Hydroxysuccinimide(NHS) layer and applied to determine the dopamine in the presence of an excess ascorbic acid using square-wave voltammetry. The electrochemical properties of the modified electrode were examined in the solution containing dopamine/ascorbic acid using cyclic voltammetry(CV): the separation between the oxidation peaks of dopamine and ascorbic acid was largely dependent on the pH of the sample solution and became maximum at pH 4.0. Hence, the square-wave voltammetric determination of dopamine was carried out in a pH 4.0, 100mM phosphate buffer saline(PBS) containing 140mM NaCl. The detection limit and response slop were improved from $1.0{\mu}M\;to\;5.0\times10^{-2}{\mu}M\;and\;from\;0.93{\mu}A/{\mu}M\;to\;6.1{\mu}A/{\mu}M$, respectively, upon modification of the electrode surface by NHS.

A Study on Chest X-ray Using Ancillary Device for Child Radiography (방사선촬영 보조기구를 이용한 어린이 흉부 엑스선 검사에 관한 연구)

  • Rhee, Do-byung;Lee, Somi;Choi, Hyunwoo;Kim, Jong-ki;Lee, Jongmin
    • Journal of Biomedical Engineering Research
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    • v.39 no.1
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    • pp.48-54
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    • 2018
  • In this study, We developed a Ancillary device for child radiography for X-ray of children under 5 years old and verified its effectiveness. Chest X-rays of children younger than 5 years of age were performed by Supine method at the position of Table detector, Short - Source to Image Receptor Distance(SID). Existing Supine and Short -SID imaging methods cause many problems, such as errors in image reading and excessive radiation exposure dose to patients, but the use of an Ancillary device for child radiography(ADCR) solves these problems. A total of 160 children were divided into the Upright group using ADCR and Supine group without ADCR. The chest X-ray image was visually evaluated by two radiologists with reference to the European Commission's List of Quality Criteria for Diagnostic Radiographic Images in Pediatrics. The total score of the qualitative evaluation was 5.15% higher in the chest upright method using ADCR than in the chest supine method without ADCR, and the chest upright method score was higher than that of the chest supine method in items 1 to 7. whether infants have deep inspiration or not, 4.87% higher for item 1, whether infants rotate or not and the degree of tilting, 0% higher for the item 2, the reproduction of image from just above apices of lungs to T12/L1, 0% for the item 3, reproduction of the vascular pattern in central 2/3 of the lungs, 6.92% higher for the item 4, reproduction of the trachea and the proximal bronchi, 12.9% higher for the item 5, visually sharp reproduction of the diaphragm and costo-phrenic angles, 10% higher for the item 6, reproduction of the spine and paraspinal structures and visualisation of the retrocardiac lung and the mediastinum, and 3.65% higher for the item 7. Items 2 and 3 showed no statistically significant differences(P > 0.05), and items 1, 4, 5, 6, and 7 showed statistically significant differences(P < 0.05). In conclusion, Upright method using ADCR in pediatric chest X-ray is considered as a good alternative to existing Supine method.

Influence of Natural Salt Treatments on Soil Chemical Properties and Inorganic Contents of Garlic (천일염 살포가 토양 화학성과 마늘(Allium sativum L.)의 무기성분 함량에 미치는 영향)

  • Kim, Myung-Sook;Kim, Yoo-Hak;Kang, Seong-Soo;Yun, Hong-Bae;Gong, Hyo-Young;Lee, Sang-Beom
    • Korean Journal of Organic Agriculture
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    • v.20 no.2
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    • pp.231-241
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    • 2012
  • Farming using natural salts for supply of nutrients to crops is increasing recently. It is necessary to evaluate the salt accumulation in soil and the effects on crop growth by treatment of natural salt. This study was conducted at the organic cultivation fields which garlics were planted. The treatments were no natural salts (control) and plots applied 100~600 $kg\;ha^{-1}$ with natural salts. Soil samples were taken from the 0 to 25 cm depth at 12 and 107 day (harvest time) after natural salts application. The results showed that electrical conductivity (EC), exchangeable Na (Exch.$-Na^+$) and Cl- were increased by application of natural salts. But, pH and exchangeable cations ($K^+$, $Ca^{2+}$ and $Mg^{2+}$) had not significantly differences among treatments. In 300 $kg\;ha^{-1}$ plots of natural salt, the level of EC, Exch.$-Na^+$ and $Cl^-$ in top soil (0-5 cm) was were increased more 0.3, 3.7, and 12.7 times than control plot, respectively. EC, Exch.$-Na^+$, and $Cl^-$ were highest in the top 5 cm of soil and decreased with depth at 12 days after natural salts application, but were decreased in the plower layer (0-15cm) at the harvest time because they were leached with natural rain. An increasing the application level of natural salt resulted in increasing of sodium adsorption ratio, exchangeable sodium percentage, and percentage of soil dispersion. The concentration of nutrient uptake such as total nitrogen (T-N), chloride (Cl) in garlic had significant difference between control and plots applied with natural salts The content of T-N of garlic in plots with natural salt application was lower than control plot, but Cl is higher than control plot.

A New process for the Solid phase Crystallization of a-Si by the thin film heaters (박막히터를 사용한 비정질 실리콘의 고상결정화)

  • 김병동;정인영;송남규;주승기
    • Journal of the Korean Vacuum Society
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    • v.12 no.3
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    • pp.168-173
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    • 2003
  • Recently, according to the rapid progress in Flat-panel-display industry, there has been a growing interest in the poly-Si process. Compared with a-Si, poly-Si offers significantly high carrier mobility, so it has many advantages to high response rate in Thin Film Transistors (TFT's). We have investigated a new process for the high temperature Solid Phase Crystallization (SPC) of a-Si films without any damages on glass substrates using thin film heater. because the thin film heater annealing method is a very rapid thermal process, it has very low thermal budget compared to the conventional furnace annealing. therefore it has some characteristics such as selective area crystallization, high temperature annealing using glass substrates. A 500 $\AA$-thick a-Si film was crystallized by the heat transferred from the resistively heated thin film heaters through $SiO_2$ intermediate layer. a 1000 $\AA$-thick $TiSi_2$ thin film confined to have 15 $\textrm{mm}^{-1}$ length and various line width from 200 to 400 $\mu\textrm{m}$ was used as the thin film heater. By this method, we successfully crystallized 500 $\AA$-thick a-Si thin films at a high temperature estimated above $850^{\circ}C$ in a few seconds without any thermal deformation of g1ass substrates. These surprising results were due to the very small thermal budget of the thin film heaters and rapid thermal behavior such as fast heating and cooling. Moreover, we investigated the time dependency of the SPC of a-Si films by observing the crystallization phenomena at every 20 seconds during annealing process. We suggests the individual managements of nucleation and grain growth steps of poly-Si in SPC of a-Si with the precise control of annealing temperature. In conclusion, we show the SPC of a-Si by the thin film heaters and many advantages of the thin film heater annealing over other processes