• Asian Pacific Journal of Cancer Prevention
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• 제17권10호
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• pp.4671-4675
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• 2016

Background and aim: Pancreatic cancer is the fourth leading cause of cancer-related death worldwide, with an overall 5-year survival of <5% mainly due to presence of advanced disease at time of diagnosis. Therefore development of valid biomarkers to diagnose pancreatic cancer in early stages is an urgent need. This study concerned the sensitivity and specificity of serum ICAM 1 versus CA 19-9 in differentiation between pancreatic cancer and healthy subjects and acohort of patients with chronic pancreatitis with a focus on assessing validity in diagnosis of early stages of pancreatic cancer. Methods: A cohort of 50 patients with histologically diagnosed pancreatic tumors, 27 patients with chronic pancreatitis, and 35 healthy controls were enrolled. Serum samples for measurement of CA19-9 and I-CAM 1 were obtained from all groups and analyzed for significance regarding diagnosis and disease stage. Results: At a cut off value of (878.5 u/ml) I-CAM 1 had 82% and 82.26% sensitivity and specificity for differentiation between cancer and non-cancer cases, with higher sensitivity and specificity than CA19-9 at different cut offs (CA19-9 sensitivity and specificity ranged from 64-80% and 56.4 - 61.2% respectively). The AUC was 0.851 for I-CAM and 0.754 for CA19-9. Neither of the markers demonstrated significance for distinguishing between early and late cancer stages. Conclusion: ICAM 1 is a useful marker in differentiation between malignant and benign pancreatic conditions, and superior to CA19-9 in this regard. However, neither of the markers can be recommended for use in differentiation between early and late stage pancreatic cancers.

• Journal of Nutrition and Health
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• 제48권4호
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• pp.327-334
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• 2015

본 연구에서는 한국 음식의 양념류, 향신료로 많이 사용해온 생강의 여러 성분 중 (6)-gingerol을 3T3-L1 preadipocyte에 처리하였을 때 지방세포의 증식과 분화되는 과정에 미치는 영향에 대해 관찰해보고자 하였다. 실험 결과 (6)-gingerol의 첨가량이 증가할수록 세포의 증식이 유의적으로 억제되었다. 지방 분화과정 중에서의 (6)-gingerol은 분화초기에는 효과가 나타나지 않았지만, 지방세포로의 변화가 가속화되는 중기 과정에 관여하는 $PPAR{\gamma}$, $C/EBP{\alpha}$는 (6)-gingerol의 처리로 두 유전자의 발현이 억제되는 것을 확인할 수 있었고, 후기 관련 유전자인 FABP4, AP2의 발현도 (6)-gingerol의 처리군에서 발현이 유의적으로 감소되었다. 또한, 지방세포에서만 분비되는 adipocytokine 중 leptin 발현에는 (6)-gingerol의 처리가 유의적으로 억제되었으나, adiponectin의 경우에는 유의적인 효과는 나타나지 않았다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권1호
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• pp.44-47
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• 2000

Cell growth and DNA synthesis were studied from a cultured early- and late- pas- sage mouse aorta smooth muscle cell (MASMC) because the proliferation of vascular smooth muscle cell (VSMC) is a key factor in development of atherosclerosis. In this study, the cells were cultured in fetal bovine serum (FBS) and stimulated by growth factors such as thrombin and platelet-derived growth factor-BB (PDGF-BB). Compared to the number of early-passage MASMC (passage 3 to 9) the number of late-passage MASMC (passage 30 to 40) in a normal serum state was increased 2 fold at Day 1, 3 and 6 in culture, respectively. Incorporation of $[^3H]$ thymidine into DNA induced by serum, PDGF and thrombin in late-passage MASMC was greater than those in early-passage MASMC. We also examined whether intracellular zinc levels would be an aging factor or not. The intracellular zinc level in early- and late-passage MASMC was monitored by using the zinc probe dye N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide. It is interested that late-passage MASMC increased the intracellular fluorescence level of zinc, more than the early passage MASMC did. The alterations of intracellular zinc level occur concurrently with changes in MASMC proliferation rate during aging. This data suggest that the age-associated changes in zinc concentrations may provide a new in vitro model for the study of smooth muscle cell differentiation.

• Applied Microscopy
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• 제31권2호
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• pp.129-141
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• 2001

식충목 땃쥐과 쇠뒤쥐 (Sarex minutus gracillimus)의 정자변태과정을 알아보기 위하여 세정관 상피의 세포분화에 따른 형태적 특징을 조사하여 다음과 같은 결과를 얻었다. 쇠뒤쥐의 정자변태과정을 골지기, 두모기를 각각 전 중 후기로, 첨체기를 각각 전 후기로, 성숙기와 이탈기를 각각 하나의 단계로 총 10단계로 나눌 수 있었다. 핵질의 응축은 첨체기에 시작하여 이탈기에 완성되었으며, 만세트의 출현은 첨체후기부터 성숙기까지 나타났다. 정자편모의 형성은 골지후기에 생성하여 이탈기에 완성되었다. 다포체는 골지기부터 성숙기까지 나타났으며, 골지기는 주로 pale multivesicular body가, 두모기와 첨체기는 주로 pale 또는 moderate pale multivesicular bodies가 그리고 성숙기에서는 dense multivesicular body가 관찰되었다.

• Journal of Animal Science and Technology
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• 제49권5호
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• pp.593-598
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• 2007

본 연구는 스테로이드 성호르몬 즉 에스트로겐, 테스토스테론 및 노르테스토스테론이 돼지 지방전구세포의 분화와 증식에 미치는 영향을 구명하기 위해서 수행하였다. 지방전구세포는 갓난 암퇘지의 등지방조직을 떼어내어 coll- agenase를 처리해서 분리해서 배양했다. 세포 배양중에 10－8M과 10－7M의 스테로이드 성호르몬을 처리했다. 세포배양 전기에 스테로이드 성호르몬을 처리했을 때 지방전구세포의 분화나 증식에 아무런 영향을 미치지 않았고, 세포배양 후기에 처리했을 때는 테스토스테론과 노르테스토스테론이 세포분화를 촉진시켰다.

• Nutrition Research and Practice
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• 제5권5호
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• pp.396-403
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• 2011

Ceramides (Cer) comprise the major constituent of sphingolipids in the epidermis and are known to play diverse roles in the outermost layers of the skin including water retention and provision of a physical barrier. In addition, they can be hydrolyzed into free sphingoid bases such as $C_{18}$ sphingosine (SO) and $C_{18}$ sphinganine (SA) or can be further metabolized to $C_{18}$ So-1-phosphate (S1P) and $C_{18}$ Sa-1-phosphate (Sa1P) in keratinocytes. The significance of ceramide metabolites emerged from studies reporting altered levels of SO and SA in skin disorders and the role of S1P and Sa1P as signaling lipids. However, the overall metabolism of sphingoid bases and their phosphates during keratinocyte differentiation remains not fully understood. Therefore, in this study, we analyzed these Cer metabolites in the process of keratinocyte differentiation. Three distinct keratinocyte differentiation stages were prepared using 0.07 mM calcium (Ca$^{2+}$) (proliferation stage), 1.2 mM Ca$^{2+}$ (early differentiation stage) in serum-free medium, or serum-containing medium with vitamin C (50 ${\mu}L$/mL) (late differentiation stage). Serum-containing medium was also used to determine whether vitamin C increases the concentrations of sphingoid bases and their phosphates. The production of sphingoid bases and their phosphates after hydrolysis by alkaline phosphatase was determined using high-performance liquid chromatography. Compared to cells treated with 0.07 mM Ca$^{2+}$, levels of SO, SA, S1P, and SA1P were not altered after treatment with 1.2 mM Ca$^{2+}$. However, in keratinocytes cultured in serum-containing medium with vitamin C, levels of SO, SA, S1P, and SA1P were dramatically higher than those in 0.07- and l.2-mM Ca$^{2+}$-treated cells; however, compared to serum-containing medium alone, vitamin C did not significantly enhance their production. Taken together, we demonstrate that late differentiation induced by vitamin C and serum was accompanied by dramatic increases in the concentration of sphingoid bases and their phosphates, although vitamin C alone had no effect on their production.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1364-1370
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• 2002

The objective of this study was to examine the effect of compensatory growth nutritional regimen on mammary gland growth and lactation. One hundred twenty-two Sprague Dawley female rats (35 days of age) were randomly assigned to either a control or a stair-step compensatory nutrition (SSCN) feeding regimen or an alternating 2-2-3-3-week schedule beginning with 40% energy restriction for 2 weeks followed by re-alimentation (control diet) for 2 weeks. Pup weight gain and milk yield were improved 8% and 8 to 15%, respectively, by the SSCN regimen. The gene expression of $\beta$-casein was 2.3-fold greater in the SSCN group than in the control group during early lactation, but they were greater at all stages of the second lactation. The gene expression of insulin-like growth factor-I was 40% lower in the SSCN group than in the control group during early lactation of the second lactation, but during late lactation it was 80% greater than in the control group. The concentration of serum corticosterone tended to be higher in the SSCN group during the late stage of the first lactation. These results suggest that the stair-step compensatory nutrition regimen improves lactation performance and persistency by modulation of cell differentiation and apoptotic cell death.

• Journal of Animal Science and Technology
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• 제46권6호
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• pp.967-974
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• 2004

본 연구는 여 러 conjugated linoleic acid(CLA) 이성체가 돼지 지방전구세포의 분화에 미치는 영향을 구영하기 위해 수행하였다. 돼지 지방전구세포는 갓난 돼지의 등 지방에서 분리해서 성숙지방세포로 분화될 때 까지 배양했다. 여러 CLA 이성체를 배양중의 세포에 처리했다. 세포분화는 세포배양이 끝난 후 세포의 glycerol-3-phosphate의 활성도를 측정함으로써 구명했다. 20$\mu$M과 50$\mu$M의 transto-cis12 CLA 이성체는 돼지 지방천구세포의 분화를 억제했고，한펀 cis9-cisl1 이성체는 세포분화를 촉진했다. cis9-trans 11 과 trans9-trans11 이생체는 세포분화에 아무런 영향을 마치지 않았다. CLA의 세포분화에 미치는 작용은 배양후기 (day8${\sim}$14) 보다 배양전기(day 0${\sim}$ 8)에 더 두드러지게 나타냈다. 위의 결과는 여러 CLA 이성체는 돼지 지방천구세포 분화에 각각 다른 작용을 가짐을 나타낸다.

• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.751-760
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• 2018

BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency. METHODS: We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM. RESULTS: We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified. CONCLUSION: Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.

• 한국지역지리학회지
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• 제9권2호
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• pp.119-134
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• 2003

본고는 우하영(禹夏永)이 저술한 "천일록(千一錄)"을 통해 18세기 말 우리 나라 농업의 지역적 특성을 살펴보는 것을 목적으로 하였다. 각 지역의 농업특성은 자연조건 농경지 분포 주요작물 농법 농업생산력 등 "천일록(千一錄)"에서 추출해 낼 수 있는 몇 가지 자료를 통해 분석하였다. 결과 당시의 농업은 오늘날과 달리 각 지역마다 뚜렷한 특성을 지니고 있었다. 그리고 이러한 농업의 지역성은 일차적으로 자연환경의 차이에 기인하여, 지형 기후 토양 등 자연조건이 농경지의 분포와 이용, 주요 작물 등을 좌우하였으며 농업생산력에도 결정적인 영향을 미치는 경우가 많았다. 각 지역의 고유한 농법 역시 농민들이 지역 나름의 자연조건을 감안하여 반복된 시행착오를 거치며 개발 발전시켜 온 것들이었다. 이와 더불어, 각 지역의 인구, 농업노동력의 양과 질, 토지소유의 다과, 수리시설을 비롯한 농업기반시설의 충실도 등사회 경제적 상황도 농업의 지역성을 심화시키는 데 지대한 역할을 하였다.