• 제목/요약/키워드: laboratory aim

검색결과 1,204건 처리시간 0.025초

A Preliminary Study on the Growth and Feeding of Rockfish, Sebastes schlegeli, in Illuminated Sea Cages

  • Park, Chul-Won;Kim, Min-Suk;Park, Yong-Joo;Kim, Jong-Man
    • Ocean and Polar Research
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    • 제23권3호
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    • pp.279-284
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    • 2001
  • The natural high productivity of temperate coastal waters in Korea can be used in advantage to reduce the feed costs for the cage-cultured marine carnivorous fish species. By using the night-lights methods an alternative of supplementary feeding strategy can be offered to the cage farmers and maintain sound environmental conditions that could enhance maximum sustainable yields. The aim of this study was to investigate the effects of night-lights which shown positive results on feeding and growth in sea cage cultured rockfish, Sebastes schlegeli. The study showed that for the water around overhead illuminated sea cage, higher zooplankton density was observed at night than during the daytime. Increased amounts of for-age, evidenced by stomach content analyses were observed in the early evening, but decreased amounts were observed in the morning and afternoon. That is, feeding activities of the fish were most intensive from midnight to dawn. In a three month feeding experiment, the results showed that night-lighted groups were superior to groups with the highest feeding efficient. This study suggested that the evidence that night-lights superimposed on only day-lights enhance growth of rockfish in sea cages during summer and winter, with timing of exposure affecting growth of juvenile fish. The capability to control the feeding behavior of marine life via manipulation and external stimuli could considerably benefit the advancement of sea cage aquaculture in coastal areas.

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Comparison of Digestive Function Among Rabbits, Guinea-Pigs, Rats and Hamsters. II. Digestive Enzymes and Hindgut Fermentation

  • Yu, Bi;Chiou, Peter Wen-Shyg;Kuo, Chung-Yi
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권11호
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    • pp.1508-1513
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    • 2000
  • The aim of this trial was to study the response of laboratory animals including omnivores (rats) and herbivores (rabbits, guinea pigs and hamsters) to the same level of dietary fiber on their digestive enzymes and hindgut fermentation. Ten weanling animals of each of four species, rabbits, guinea-pigs, rats and Syrian hamster, were fed a basal diet of 18% crude protein and 10% crude fiber for six weeks. The digesta and tissue of each intestinal segment were collected to measure the activity of digestive enzymes. Rabbits contained the highest secreted pepsin activity in the stomach, whereas rats contained the highest protease and ${\alpha}-amylase$ activity in the small intestine, and lower fibrous hydrolases in the hindgut than rabbits, guinea pigs and hamsters. The total VFA productions in the caecum and colon were highest in rats, followed by hamsters and rabbits, while the guinea pigs contained the lowest VFA and a different pattern of VFA molar ratio from the other laboratory animals. The degree of hindgut fermentation in these laboratory animals was in reverse to the trend for their fiber digestion.

Expression of Fragile Histidine Triad (FHIT) and WW-Domain Oxidoreductase Gene (WWOX) in Nasopharyngeal Carcinoma

  • Chen, Xu;Li, Ping;Yang, Zheng;Mo, Wu-Ning
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권1호
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    • pp.165-171
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    • 2013
  • The aim of the present study was to analyze the expression of FHIT and WWOX in nasopharyngeal carcinoma (NPC) and correlations with clinical pathologic features. mRNA expression of the FHIT and WWOX was assessed by real-time fluorescent relatively quantitative PCR in 61 NPC tissues and 45 non-cancerous nasopharyngeal tissues. As a result, mRNA expression levels of both FHIT and WWOX were significantly lower in NPC patients than in control samples (P=0.049 and 0.045, respectively). Moreover, the mRNA expression of both had an inverse relation with larger invasive range (P=0.035 and 0.048, respectively), poor histologic differentiation (P=0.012 and 0.016) and advanced clinical stage (P=0.026 and 0.038). Consistency was found between expression of FHIT and WWOX in the same NPC tissues (r=0.681, P=0.00). In conclusion, synergy between FHIT and WWOX may exist in the development of NPC so that the two factors may be considered as important genetic markers. Detecting the expression of FHIT and WWOX should provide clinically significant information relevatn to tumor diagnosis, progression and treatment modalities for NPC.

Antioxidant Activity of Talinum portulacifolium (Forssk) Leaf Extracts

  • Rao, Thalapaneni Nageswara;Kumarappan, Chidambaram;Lakshmi, Sabapathi Mohana;Mandal, Subhash C
    • Advances in Traditional Medicine
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    • 제8권4호
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    • pp.329-338
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    • 2008
  • The aim of the study is to investigate the antioxidant activity through, reducing power, 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH), nitric oxide radical (NO), superoxide radical, hydrogen peroxide radical ($H_2O_2$) scavenging activity and the amount of total phenolic compounds of chloroform, ethyl acetate, methanol and aqueous extracts of the leaves of Talinum portulacifolium. Chloroform extract of leaves of T. portulacifolium showed highest antioxidant activity, with a direct relationship between activity and concentration of extracts ($15-240\;{\mu}g/mL$). Among all the extracts, the highest amount of the total polyphenolic compounds was found in the chloroform extract. Chloroform extract of T. portulacifolium showed an important free radical scavenging activity towards the DPPH, NO, Superoxide and $H_2O_2$ radicals, with $IC_{50}$ values of 133.26, 165.75, 156.34 and $135.29\;{\mu}g/mL$, respectively. In the lipid peroxidation assay, extracts of chloroform and ethyl acetate showed a remarkable inhibitory activity. The extracts showed significant activity in all the experiments but lower than the standard antioxidant, ascorbic acid.

Physiological Influence of Combined Mental Activity with Experimental Subway Noise

  • ;;최석철
    • 대한의생명과학회지
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    • 제13권1호
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    • pp.39-45
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    • 2007
  • Numerous psychological stressors playa role in development of the cardiovascular disease. The aim of this study was to determine whether a combined mental activity with experimental subway noise affects hematological physiology. Fifty-four healthy volunteers were divided group I which underwent subway noise (n=24) and group II which underwent a combined mental activity with subway noise (n=30). Venous blood samples were collected for measuring CBC, prothrombine time (PT), activated partial thromboplastine time (APTT), erythrocyte sedimentation rate (ESR), D-dimer and high sensitive C-reactive protein (H-CRP) levels before, 50 min of stress task (S-50m) and 60 min of recovery (R-60m). Changed ratios of granulocyte, lymphocyte, monocyte and platelet counts at S-50m and R-60m were higher in group II compared to group I. RBC count and hematocrit level in group I increased whereas those in group II decreased at S-50m. PT, APTT and ESR in the both groups were shortened at R-60m and the decreased ratios were high in group II compared to group I. H-CRP and D-dimer in the both groups were elevated at S-50m and R-60m while the increased ratios in group II were greater than those in group I. These observations imply that a combined mental activity with experimental subway noise may be a stressor which affects hematological physiology.

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Prevalence of Aspirin Resistance and Clinical Characteristics in Patients with Cerebral Infarction

  • Choi, Jong-Tae;Shin, Kyung-A;Kim, Young-Kwon
    • 대한의생명과학회지
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    • 제19권3호
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    • pp.233-238
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    • 2013
  • Aspirin is still the mainstay of antiplatelet therapy in the cardiovascular and cerebrovascular disease. However, some patients are not responsive to the antithrombotic action of aspirin. The aim of this study was to assess the prevalence and clinical characteristics of aspirin resistance in patients with cerebral infarction. We tested platelet function in 557 patients who had been treated with aspirin in J general hospital. Platelet function was tested using the multiple electrode platelet aggregometry (MEA). Platelet reactivity was expressed as area under the aggregation curve (AUC, U) and >30 AUC was defined as aspirin resistance. Aspirin resistance was detected in 16.2% patients. There was not any significant differences in age, gender between aspirin resistance and aspirin sensitive patients. WBC was significantly higher in patients with aspirin resistance (P < .05). HDL-cholesterol was significantly higher in patients with aspirin sensitive (P < .05). Aspirin resistance was positive correlation with platelet count (r =.314, P =.003). The prevalence of aspirin resistance in cerebral infarction was 16.2%, and platelet count were related with aspirin resistance.

Rapid Detection of Vancomycin-resistant Enterococci (VRE) in Clinical Samples from University Hospital

  • Yang, Byoung-Seon;Park, Jung-Yeon;Choi, Seung-Gu
    • 대한임상검사과학회지
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    • 제45권1호
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    • pp.16-20
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    • 2013
  • Outbreaks of vancomycin-resistant enterococci (VRE) are being reported more frequently in many countries. While seven glycopeptide resistance genotypes have been described in Enterococci, vanA and vanB are the most common resistance genotypes. The aim of this study was to detect antibiotic susceptibilities of 23 Enterococcus faecium strains, which caused an outbreak in a University hospital by a disk diffusion test to investigate the presence of the species specific gene, and the resistant genotypes, vanA and vanB by duplex PCR. PCR for vanA and vanB was performed on 23 enterococci. Twenty three were identified as E. faecium and were tested positive for the vanA genotype. This study will report on the validation of a simple and accurate VRE detection method that can be easily incorporated into the daily routine of a clinical laboratory. Early detection of VRE strains, including those with susceptibility to vancomycin, is of paramount clinical importance as it allows rapid initiation of strict infection control practices, as well as the therapeutic guidance for confirmed infections. The PCR method developed in the present study is simple and reliable for the rapid characterization of VRE.

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Ultrasonic treatment of waste livestock blood for enhancement of solubilization

  • Jeon, Yong-Woo;Kim, Hyeon-Jeong;Shin, Myung-Seop;Pak, Seo-Hyun
    • Environmental Engineering Research
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    • 제21권1호
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    • pp.22-28
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    • 2016
  • The aim of this study was to recycle the waste livestock blood as one of the waste biomass by turning proteins, the main constituent of blood, into effective biological resources like amino acid. Ultrasonic technology was applied to solubilize the proteins in the waste livestock blood. And of the multiple ultrasonic frequencies tested, 20 kHz was confirmed to yield the highest solubilization rate. The optimum pretreatment conditions were determined to be 30-min treatment at an ultrasonic irradiation density of 0.5 W/mL, which resulted in a solubilization rate of 96.01%. Also, a gel permeation chromatography (GPC) confirmed that a large amount of proteins were solubilized, and in an experiment where ultrasonic treatment was applied to kill bacteria, death rates of general bacteria and total coliforms were found to be reduced by 99.93% and 100%, respectively. Based on these results, ultrasonic technology was confirmed to be a crucial part of treating and recycling the proteins in waste livestock blood.

경북지역 재래염소의 Coxiella burnetii 항체보유율 (Seroprevalence of Coxiella burnetii in native Korean goats (Capra hircus coreanae) in Gyeongbuk province, Korea)

  • 김성국;조재청;이민교;김선수;이승헌;곽동미
    • 한국동물위생학회지
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    • 제37권4호
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    • pp.241-246
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    • 2014
  • Q fever is a zoonotic disease caused by the obligate intracellular bacterium Coxiella (C.) burnetii and affects wild and domestic animals worldwide. The aim of this study was to evaluate the seroprevalence of C. burnetii in native Korean goat (Capra hircus coreanae) in Gyeongbuk province, Korea, using ELISA. A total of 256 goat blood samples from 56 farms in Gyeongbuk province were collected between May 2012 and March 2013. Among them, 22 (8.6%) samples from 10 (17.9%) farms were seropositive for C. burnetii by ELISA. According to regional analysis, the seroprevalences among goat farms in eastern, western, southern, and northern areas of Gyeongbuk province were 0%, 18.2%, 36.8%, and 6.3%, respectively, showing the highest seroprevalence in the southern region. Among 22 counties in Gyeongbuk province, 10 (45.5%) counties had one or more farms positive to C. burnetii antibody. Accordingly, the seroprevalence of C. burnetii in high-risk humans and animals are constantly demanded by regional investigation.

중·고등학교의 교사업무공간에 관한 연구 - 교과교실형 설계기반 중·고등학교를 중심으로 - (A study on Teacher's Work Space in Middle and High School - Focus on Plan Based Department System Management in Middle and High School -)

  • 서붕교;곽준혁
    • 교육시설 논문지
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    • 제21권1호
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    • pp.23-32
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    • 2014
  • Aim of this research is suggesting a satisfied studying space for lecturer, student and curriculum by abstraction of improvement plan which based on investigation and analysis of the space that teacher is using in lecturer and study support facility in school which managed in 'Department system'. This research focused on analyse of teacher's laboratory, teacher's room, and common room in middle and high school which managed and designed by 'Department system' and registered on 'Seoul office of education' and designed in management of department system between 2009 and 2012. With these process, a conclusion of this research is as in the following. Firstly, plan of teachers' private place which is efficient and stable is required through to get out of module which can cover subject band and grade band. Secondly, we have to focus on size of teachers' convenience facilities for convenience or official usage, movement radius, and improvement of working environments. Thirdly, 'Subject type teachers' laboratory' which organized by teachers who teach same subject is more efficient than 'Task type laboratory' which organized by teachers who working on same task for management of school. In fourth, 'Task type teachers' laboratory' arranged regardless with teachers' class room to teach, and it makes lack of connectivity between teacher and class room.