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Establishment of Culture System of a New Strain NT0423 of Bacillus thuringiensis (새로운 Bacillus thuringiensis NT0423 균주의 배양체계)

  • 김호산;노종열;이대원;우수동;강석권
    • Korean journal of applied entomology
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    • v.37 no.2
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    • pp.187-191
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    • 1998
  • For efficient and economical production of Btrc,illus tlz~rr.ingi~r1~sstirsa in NT0423 as amicrobial-control agents, a new culture medium and culture condition were developed. Five mediadesignatzd as SWI I , SW14, SW23. SW32 and SW4I were prepared ~ : i t hv arious mixture ratio ofsoybean cake and wheat bran. It was founcl that in terms of the cell growth rate and development ofsporulation of B, thrri.il~girrl.sis strain NT0423 in all SW culture media were more efficient than those inGYS and in LB media. Total cell number in all SW media showed similar values, hut SW32 lnediilm wasthe most efficient in the development of spore, which amo~~ntetod 3.7 x 10XC FUImI. Also. at the pHranging frorn 6.2 to 7.3 in the mediiun~ no ad\:erse effect was not made in the culture of B. thur-ingicnsisstrain NT0423. The optimal volume (%) of SW32 mecliuni in a 5 1 fernientor was determined as 4 8\rolume of total niediuni. resulting ill growth (4.2 x 1OTCFUlrnl) of H. t1~~irir1,yirrz.ssit.vr ain NT0423. As H.t l i ~ t r i t ~ g iw~ a~s~ csuil~tu rcd in the shakc-flash and 5 1 fcrnientor. bacterial cells were yielded to 1 X 10"CFUIml and 5 x I O1oCFLJlml.FUIml and 5 x I O1oCFLJlml.

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Effect of Ozone Treatment for Safety Improvement of Fresh Vegetable Juice (녹즙의 위생화를 위한 오존처리의 효과)

  • Cho, Jae-Min;Kwon, Sang-Chul;Tu, Gi;Jeong, Jae-Hyun;Lee, Kyung-Haeng
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.5
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    • pp.612-617
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    • 2009
  • Fresh vegetable juice is a non-heat treated product and the only step to reduce microbial growth is washing. Therefore, the materials for fresh vegetable juice including Angelica keiskei, Brassica loeracea var. acephala, and Daucus carota L. were treated by ozone after the first washing process and investigated for microbial and chemical changes. The number of the total aerobic bacteria in materials after selection step were $8.2{\times}10^5{\sim}5.0{\times}10^6\;CFU/g$, which was a higher contamination level than the limit of Korea food code ($10^5\;CFU/g$). However, after the 1st washing process and ozone treatment, the total aerobic bacterial number was reduced to $4.7{\times}10^4{\sim}6.7{\times}10^4\;CFU/g$, which showed 2 log microbial reduction. After the 2nd washing step followed by ozone treatment, there was no difference in microbial number. The number of colifroms in the materials of fresh vegetable juice were $8.0{\times}10^3{\sim}3.5{\times}10^3\;CFU/g$ initially but showed $1.5{\times}10^2{\sim}3.0{\times}10^2\;CFU/g$ after the ozone treatment (1 log reduction). On the other hand, there was no changes in the contents of ascorbic acid, flavonoids, polyphenols, minerals (cadmium and lead) during all processes. In addition, no color changes were observed during washing process. Therefore, ozone treatment in the materials of fresh vegetable juice decreased the microbial numbers. Also, chemical characteristics of ozone treated sample were not different when compared with control.

A MPEG Audio-Visual Conversational Communication Terminal on the B-ISDN Environment (광대역 ISDN용 MPEG 오디오-비쥬열 대화형 통신단말의 설계 및 구현)

  • Hwang, Dae-Hwan;Cho, Kyu-Seob
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.8
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    • pp.1960-1971
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    • 1998
  • The researches and developments to provide multimedia communication services such as Video on Demand(VoDJ), real time video phonc and multipoint vidco conferencing on broadband ISDN environmcnts have been proceeded with activity. Specifications for Vol) services which is worked by Digital Audio-Visual Council(DAVIC) to support detail technologies including total service system that is consist of VoD server. delive[\! networl, and Set-Top Box(STB) had been already finished and ITU-T SG16 also recommended the standards of H.300 series terminal aspects for conversational multimedia services, But the architectures of multimedia tenninals recommended and specified by these organizations do not have an efficient st11lcture to provide all of retrieval, distrihution and conversational service due to a different point of view about multimedia terminals and services. In this paper, we analyzed the recornmendatio!E and the specifications of intemational public and private organizations like lTU-T, DAVIC and ATM forum. As a result of these analysis. we propose an efficient terminal architecture, and then we have designed, lmplemented the multimedia communication terminal for offering VoI) and real- time conversation ,,, functional module test according to the individual commumication service session and confirined the validiry or terminal implemented to be used on broadband ISDK environments.

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Effect of lactic acid bacteria and yeast supplementation on anti-nutritional factors and chemical composition of fermented total mixed ration containing cottonseed meal or rapeseed meal

  • Yusuf, Hassan Ali;Piao, Minyu;Ma, Tao;Huo, Ruiying;Tu, Yan
    • Animal Bioscience
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    • v.35 no.4
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    • pp.556-566
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    • 2022
  • Objective: This study aimed to determine the appropriate supplementation level of lactic acid bacteria (LAB; Lactobacillus plantarum and Bacillus clausii), yeast (Saccharomyces cariocanus and Wickerhamomyces anomalus) for degrading free gossypol and glucosinolate in the fermented total mixed ration (TMR) containing cottonseed meal (CSM) or rapeseed meal (RSM), to improve the utilization efficiency of these protein sources. Methods: For LAB, L. plantarum or B. clausii was inoculated at 1.0×108, 1.0×109, 1.0×1010, and 1.0×1011 colony-forming unit (CFU)/kg dry matter (DM), respectively. For yeast, S. cariocanus or W. anomalus was inoculated at 5×106, 5×107, 5×108, and 5×109 CFU/kg DM, respectively. The TMR had 50% moisture and was incubated at 30℃ for 48 h. After fermentation, the chemical compositions, and the contents of free gossypol and glucosinolate were determined. Results: The results showed that the concentration of free gossypol content was reduced (p<0.05), while that of the crude protein content was increased (p<0.05) in the TMR containing CSM inoculated by B. clausii (1×109 CFU/kg DM) or S. cariocanus (5×109 CFU/kg DM). Similarly, the content of glucosinolate was lowered (p<0.05) and the crude protein content was increased (p<0.05) in TMR containing RSM inoculated with B. clausii (1×1010 CFU/kg DM) or S. cariocanus (5×109 CFU/g DM). Conclusion: This study confirmed that inclusion of B. clausii with 1.0×109 or 1.0×1010 CFU/kg DM, or S. cariocanus (5×109 CFU/kg DM) to TMR containing CSM/RSM improved the nutritional value and decreased the contents of anti-nutritional factors.

Radiation stability and radiolysis mechanism of hydroxyurea in HNO3 solution: Alpha, beta, and gamma irradiations

  • Yilin Qin;Wei Liao;Tu Lan;Fengzhen Li;Feize Li;Jijun Yang;Jiali Liao;Yuanyou Yang;Ning Liu
    • Nuclear Engineering and Technology
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    • v.54 no.12
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    • pp.4660-4670
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    • 2022
  • Hydroxyurea (HU) is a novel salt-free reductant used potentially for the separation of U/Pu in the advanced PUREX process. In this work, the radiation stability of HU were systematically investigated in solution by examining the effects of the type of rays (α, β, and γ irradiations), the absorbed dose (10-50 kGy), and the HNO3 concentration (0-3 mol L-1). The influence degree on HU radiolysis rates followed the order of the absorbed dose > the ray type > the HNO3 concentration, but the latter two had moderate effects on HU radiolysis products where NH4+ and NO2- were found to be the most abundant ones, suggesting that the differences of α, β, and γ rays should be considered in the study of irradiation effects. The radiolysis mechanism was explored using density functional theory (DFT) calculations, and it proposed the dominant radiolysis paths of HU, indicating that the radiolysis of HU was mainly a free radical reaction among ·H, eaq-, H2O, intermediates, and the radiolytic free radical fragments of HU. The results reported here provide valuable insights into the mechanistic understanding of HU radiolysis under α, β, and γ irradiations and reliable data support for the application of HU in the reprocessing of spent fuel.

Chemical Composition and Immunostimulating Activity of the Fermented Korean Ginseng (Panax ginseng C.A. Meyer) with Mushroom Mycelium by Solid Culture (균사체를 이용한 수삼 고체발효물의 화학적 조성 및 면역 활성)

  • Park, Chang-Kyu;Kim, Hoon;Tu, Qi;Yu, Kwang-Won;Jeong, Heon-Sang;Lee, Hyeon-Yong;Jeong, Jae-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.9
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    • pp.1145-1152
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    • 2009
  • For the utilization of Korean ginseng (Panax ginseng C.A. Meyer) in the functional drink, we prepared the fermented Korean ginseng with mushroom mycelia (Ganoderma lucidum; WG-GL, Hericium erinaceum; WG-HE and Phellinus linteus; WG-PL) by solid culture. A proximate analysis showed that the fermented Korean ginseng contained significantly more crude fat (4.66$\sim$12.02%) than Korean ginseng (WG, 1.61%) whereas crude protein content of WG (13.64%) was higher value than those of the ferments (7.60$\sim$12.57%). When we also evaluated effects of the fermented Korean ginseng on the mitogenic activity, hot-water extract from WG-PL was significantly higher than those of WG or mycelia only fermentation (GL, HE and PL) as analyzed by IL-2 production (1.64-fold of the saline control) and proliferation of splenocytes (1.47-fold). In addition, the lysosomal phosphatase activity (WG-HE; 1.32-fold) and NO/TNF-$\alpha$ production (WG-HE; 2.27-fold of the saline control at 50 ${\mu}g$/mL, WG-PL; 3.56-fold, respectively) from macrophage in the presence of the fermented Korean ginseng were higher than those of WG or mycelia fermentation. These results indicate that hot-water extracts from the fermented Korean ginseng with mushroom mycelia by solid culture contain chemical ingredients different from the Korean ginseng, and that it might provide beneficial immunostimulating activity.

Monitoring of Trans Fatty Acid and Cholesterol of Bakery Products Sold at Retail in Seoul Area (서울지역 소매업체 제빵류의 트랜스지방 및 콜레스테롤 조사)

  • Park, Young-Hye;Kang, Sung-Tae;Hwang, Young-Ok;Tu, Ock-Ju;Shin, Jae-Min;Lee, Kyeong-Ah;Shin, Ki-Young;Chae, Young-Zoo
    • Journal of Food Hygiene and Safety
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    • v.26 no.3
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    • pp.227-234
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    • 2011
  • The objective of this study was to analyze contents of trans fatty acid and cholesterol of bakery products (bread: 17, pastry: 20, and whipping cream cake: 17) sold at retail in Seoul area. The average values of crude fat contents in bakery products were as follows [mean (minimum-maximum), %)]; bread 6.46 (3.51~8.69), pastry 16.23 (3.55~25.56), and whipping cream cake 16.26 (8.61~31.58). Palmitic acid was the most abundant fatty acid in these products. The average values of saturated fat (SFA) contents and unsaturated fat acid (USFA) contents in these items were as follows [(mean SFA ${\pm}$ SD%] bread (54.04 ${\pm}$ 5.27), pastry (53.41 ${\pm}$ 4.80), and whipping cream cake(70.09 ${\pm}$ 8.64). The high contents of trans fatty acid was analyzed in whipping cream cake. The average values of cholesterol contents in bakery products were as follows [mean (minimum-maximum), %)]; bread 2,36 (0.0~12.86), pastry 8.11 (0.0~42.80), and whipping cream cake 30.55(0.0~132.99).

The Fatty Acid Composition and Trans Fatty Acid Contents of Bakery Products in Seoul (서울시 일부 제과점 제품의 지방산 조성 및 트랜스지방 함량 분석)

  • Tu, Ock-Ju;Kim, Ae-Kyeong;Kim, Mi-Sun;Park, Young-Hye;Park, Hea-Won;Kim, Yeon-Cheon;Chae, Young-Zoo
    • Journal of Food Hygiene and Safety
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    • v.26 no.2
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    • pp.160-165
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    • 2011
  • The composition of fatty acids in bakery products from 35 bakeries in Seoul was analyzed by GCFID. The contents of crude fat in bakery products were represented 9.54-44.30% in pastries, 14.67-41.22% in cookies and 7.63-28.15% in whipped cream cakes. The total saturated fatty acid content in pastries, cookies and whipped cream cakes were 12.8-75.1 %, 8.7-81.6% and 50.2-85.9% respectively. The proportion of trans fatty acids in pastries ranged from 0.00 to 3.37%. Cookies and whipped cream cakes were represented varying amount of trans fatty acid with highest value of 4.55% in cookies and 2.13% in whipped cream cakes. Total estimated daily intakes of fatty acids from pastries and whipped cream cakes were as follows: 1.2 g/day/person for saturated fatty acids and 0.014 g/day/person for trans fatty acids.