• Title/Summary/Keyword: lTU

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Ecotoxicity Assessment of 1,4-Dioxane and Dichloromethane in Industrial Effluent Using Daphnia magna (물벼룩을 이용한 산업방류수 중 1,4-다이옥산 및 디클로로메탄의 생태독성평가)

  • Choi, Jae Won;Lee, Sun Hee;Lee, Hak Sung
    • Applied Chemistry for Engineering
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    • v.30 no.4
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    • pp.466-471
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    • 2019
  • 1,4-dioxane and dichloromethane are classified as carcinogenic groups in the International Agency for Research on Cancer (IARC). They are frequently released at high concentrations in an industrial wastewater effluent. The acute toxicity (24 h) of Daphnia magna for 7.53 mg/L of 1,4-dioxane in the industrial effluent was evaluated as 1.1 TU (toxic unit) and showed TU close to the effluent quality standard. Mixed substances of 1,4-dioxane and dichloromethane in the industrial effluent showed relatively high TU as compared to that of a single substance. Half maximal effective concentration (24 h $EC_{50}$) values of 1,4-dioxane and dichloromethane for the synthetic wastewater prepared in laboratory were 1,744 (0.06 TU) and 170 mg/L (0.6 TU), respectively and the toxicity was low. Nevertheless the toxicological evaluation of the mixture showed that TU values increased to 0.02, 0.04 and 0.10, respectively as 1, 5 and 10 ppm of dichloromethane was added to 100 ppm of 1,4-dioxane. And the synergistic effect was observed between two substances. But the TU value of synthetic wastewater was below 5%, lower than that of industrial effluent at the similar concentration.

Characterization of a Blend-Biosurfactant of Glycolipid and Lipopeptide Produced by Bacillus subtilis TU2 Isolated from Underground Oil-Extraction Wastewater

  • Cheng, Fangyu;Tang, Cheng;Yang, Huan;Yu, Huimin;Chen, Yu;Shen, Zhongyao
    • Journal of Microbiology and Biotechnology
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    • v.23 no.3
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    • pp.390-396
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    • 2013
  • Biosurfactants have versatile properties and potential industrial applications. A new producer, B. subtilis TU2, was isolated from the underground oil-extraction wastewater of Shengli Oilfield, China. Preliminary flask culture showed that the titer of biosurfactant obtained from the broth of TU2 was ~1.5 g/l at 48 h (718 mg/l after purification), with a reduced surface tension of 32.5 mN/m. The critical micelle concentration was measured as 50 mg/l and the surface tension maintained stability in solution with 50 g/l NaCl and 16 g/l $CaCl_2$ after 5 days of incubation at $70^{\circ}C$. FT-IR spectra exhibited the structure information of both glycolipid and lipopeptide. MALDI-TOF-MS analyses confirmed that the biosurfactant produced by B. subtilis TU2 was a blend of glycolipid and lipopeptide, including rhamnolipid, surfactin, and fengycin. The blended biosurfactant showed 86% of oil-washing efficiency and fine emulsification activity on crude oil, suggesting its potential application in enhanced oil recovery.

Resistance Evaluation of Radish (Raphanus sativus L.) Inbred Lines against Turnip mosaic virus (순무모자이크바이러스에 대한 무 육종 계통 저항성 평가)

  • Yoon, Ju-Yeon;Choi, Gug-Seoun;Kim, Su;Choi, Seung-Kook
    • Research in Plant Disease
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    • v.23 no.1
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    • pp.60-64
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    • 2017
  • Leaves of twenties radish (Raphanus sativus L.) inbred lines were mechanically inoculated with Turnip mosaic virus (TuMV) strain HY to evaluate TuMV resistance of the radish inbred lines. The inoculated radish plants were incubated at $22^{\circ}C{\pm}3^{\circ}C$ and resistance assessment was examined using symptom development for 4 weeks. Based on the reactions of differential radish inbred lines, 16 radish lines were produced mild mosaic, mottling, mosaic and severe mosaic symptoms by TuMV infection. These results were confirmed by RT-PCR analysis of TuMV coat protein gene, suggesting that TuMV is responsible for the disease symptoms. Four resistant radish lines did not induce systemic mosaic symptoms on upper leaves and chlorosis in stem tissues for 4 weeks, showing they were symptomless by 8 weeks. Further examination of TuMV infection in the 4 radish lines showed no TuMV infection in all systemic leaves. These results suggest that the 4 radish lines are highly resistant to TuMV.

The subtle effect of integral scale on the drag of a circular cylinder in turbulent cross flow

  • Younis, Nibras;Ting, David S.K.
    • Wind and Structures
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    • v.15 no.6
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    • pp.463-480
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    • 2012
  • The effects of Reynolds number (Re), freestream turbulence intensity (Tu) and integral length scale (${\Lambda}$) on the drag coefficient ($C_d$) of a circular cylinder in cross flow were experimentally studied for $6.45{\times}10^3$ < Re < $1.82{\times}10^4$. With the help of orificed plates, Tu was fixed at approximately 0.5%, 5%, 7% and 9% and the normalized integral length scale (L/D) was varied from 0.35 to 1.05. Our turbulent results confirmed the general trend of decreasing $C_d$ with increasing Tu. The effectiveness of Tu in reducing $C_d$ is found to lessen with increasing ${\Lambda}$/D. Most interestingly, freestream turbulence of low Tu (${\approx}5%$) and large ${\Lambda}$/D (${\approx}1.05$) can increase the $C_d$ above the corresponding smooth flow value.

Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

  • Li, Guoliang;Zhang, Shifan;Li, Fei;Zhang, Hui;Zhang, Shujiang;Zhao, Jianjun;Sun, Rifei
    • The Plant Pathology Journal
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    • v.37 no.1
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    • pp.47-56
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    • 2021
  • Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

L4 - Virtualization and Beyond

  • Hartig, Hermann;Roitzsch, Michael;Bottcher, Alexander;Lackorzynski, Adam;Dobel, Bjorn
    • Communications of the Korean Institute of Information Scientists and Engineers
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    • v.26 no.10
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    • pp.28-40
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    • 2008

Investigation of Eco-toxicological Substances in Banwol Industrial Drainages using the Regression Analysis (회귀분석을 이용한 반월 공단배수의 생태독성 원인물질 조사)

  • Kim, Yo-Yong;Woo, Jung-Sik;Hwang, Sun-Min;Kim, Moon-Jeong
    • Journal of Korean Society on Water Environment
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    • v.28 no.3
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    • pp.467-472
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    • 2012
  • In this study, water quality and acute toxicity using Daphnia magna were analyzed to investigate the eco-toxicological substances identified as statistical analysis and propose a management plan for the effluent of Banwol industrial complex, Ansan, Gyeonggi-do. Cu, Zn, F, Mn concentrations in the effluent far exceeded the US standards to protect the aquatic ecosystem and eco-toxicity values were 5 ~ 22 TU. However, concentrations of heavy metals significantly decreased after Ansan public wastewater treatment plant operating a biological treatment and toxicity values were 0 TU. Zn, Cr, F and Cu in the effluent showed very strong and strong positive correlations with eco-toxicity values, respectively. Regression analysis resulted in an equation between toxicity and Zn, TU = $4.884{\times}Zn$ (mg/L) -0.391 showing Zn concentration should be managed less than 0.285 mg/L to keep the eco-toxicity (TU) less than 1.

The Reactivity to 2TU PPD Tuberculin Skin Test after Percutaneous Multiple Puncture BCG Vaccination (건강한 영아에서 경피다자법 BCG 접종후 2TU 투베르쿨린 검사의 반응성)

  • Roh, Hye Ok;Lee, Woo Gill
    • Pediatric Infection and Vaccine
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    • v.6 no.1
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    • pp.101-106
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    • 1999
  • Purpose : Tuberculosis, a major public health problem, is an important cause of childhood infectious diseases. To decrease the tuberculosis morbidity rate, BCG vaccination and chemoprophylaxis are performed. Recently 2TU PPD skin test was introduced as a diagnostic method for tuberculous infection. We studied the positive conversion rate of 2TU PPD test after percutaneous multiple puncture BCG vaccination. Methods : Four hundred seventy six infants from well baby clinic of Samsung Cheil Hospital were enrolled. They were immunized with percutaneous multiple puncture technique BCG(Japan BCG laboratory, Japan) at 1 month of age. Approximately 6 months later, tuberculin skin test using RT23 2TU PPD was performed. Induration size, family history of tuberculosis and number of BCG scars were evaluated. Induration greater than or equal to 5mm was defined as positive conversion. Results : Among 476 infants, 248(52.1%) were male and 228(47.9%) were female. PPD skin tests were performed $6.2{\pm}0.5$($mean{\pm}S.D.$) months after BCG vaccination. Mean induration size was $7.3{\pm}3.2mm$ and positive conversion rate was 85.5%. Total number of BCG scars was $15.5{\pm}3.2$. Conclusion : The seroconversion rate by 2TU PPD test after percutaneous multiple puncture BCG vaccination was high. But, more comparative studies with various age groups may be needed for 2TU PPD test used as diagnostic method of tuberculosis in the hospitals.

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