• 제목/요약/키워드: lL-4 polymorphism

검색결과 153건 처리시간 0.022초

Chloroplast DNA Spacers로 분석한 국내 Rubus 재배종의 계통학적 유연관계 (Phylogenic Relationship of Rubus Cultivated in Korea Revealed by Chloroplast DNA Spacers)

  • 유기석;박명렬;백소현;윤성중
    • 한국약용작물학회지
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    • 제18권4호
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    • pp.266-272
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    • 2010
  • There is a considerable difference in morphological traits between Bokbunja cultivated in Korea (KCB) and Korea native Rubus coreanus, contrary to the conviction that the cultivated Bokbunja is the domestication of R. coreanus. To infer the phylogenetic relationship of KCB with other Rubus species, we compared the chloroplast DNA spacers of KCB with those of several Rubus species including black raspberry, R. occidentalis. The three chloroplast DNA spacers, atpB~rbcL, trnL~trnF, and trnT~trnL, were amplified using the specific primer pairs and converted to Single Strand Conformational Polymorphism (SSCP) markers. The SSCP makers of the chloroplast DNA spacers showed a considerable variation both within and among Rubus species. In the phylogenetic tree generated by the SSCP markers, KCB accessions were located in the same clade with R. occidentalis, but R. coreanus accessions in the different clade. Also, in the phylogenetic tree by the nucleotide sequences of the chloroplast DNA spacer trnL~trnF, KCB located in the same clade with R. occidentalis but not with R. coreanus. These results suggest that the three KCB accessions share higher similarity with R. occidentalis than with R. coreanus in the three chloroplast DNA spacers.

Molecular identification of oriental medicinal plant Schizonepeta tenuifolia bunge (Hyung-Gae) by multiplex PCR

  • Jigden, Baigalmaa;Wang, Hongtao;Kim, Yeon-Ju;Samdan, Narantuya;In, Jun-Gyo;Yang, Deok-Chun
    • Plant Biotechnology Reports
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    • 제4권3호
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    • pp.223-228
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    • 2010
  • Schizonepeta tenuifolia (Korean name "Hyung- Gae") is an oriental medicinal plant that is widely used in Korea, China and Japan. S. tenuifolia (Hyung-Gae) has many pharmacological activities and is mostly used for many medicinal preparations. The dried aerial part (spikes and stems) of three oriental medicinal plants, S. tenuifolia (Hyung-Gae), Agastache rugosa (Kwhak-Hyang) and Elsholtzia ciliata (Hyang-Yoo) belonging to the same family, mint family Labiaceae, have such similar shape and smell that it is difficult to differentiate between them. The trnL-F regions of chloroplast DNA of the three medicinal plants were sequenced and used as targets in multiplex PCR reaction to identify S. tenuifolia. After alignment of trnL-F sequences of the authenticated plant samples, one single nucleotide polymorphism (SNP) specific to S. tenuifolia was found. Based on this SNP, a new primer was designed that specifically amplifies the trnL-F region of S. tenuifolia. The established multiplex-PCR was proven to be effective in the differentiation of commercial S. tenuifolia samples from A. rugosa and E. ciliata. This rapid and accurate molecular method is highly promising for use in the food industry.

대학생의 UCP2 유전자 다형성과 식습관 비교연구 (Comparison of UCP2 Polymorphism and Dietary Habits in University Students)

  • 김경희;박미원
    • 한국식생활문화학회지
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    • 제24권2호
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    • pp.224-235
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    • 2009
  • This study aimed to research meal quality and the dietary behaviors of college students for desirable dietary lives and provides basic data for nutritional education by examining polymorphism distribution of the UCP2 gene according to gender, by investigating attitudes in terms of their dietary habits and dietary lives, and by analyzing serum lipid levels and body composition. A survey was conducted with a total of 222 students - 93 male and 129 females. Based on a selfreporting method, the questionnaires were answered over 20 minutes, and UCP2 insertion/deletion gene polymorphism and blood samples were also analyzed. The results showed that the male students and female students had average BMI of 22.50 and $20.73\;kg/m^2$, respectively. According to answers regarding their dietary lives, 51.4% of the students showed 'irregular eating' patterns, which is regarded as something to be corrected. In terms of eating regularity, 51.6% of the male students and 59.7% of the female students had irregular meal schedules. As the most important meal of a day, 64.0% of the students answered 'breakfast' but only 53.6% answered that they ate breakfast everyday. In addition, 39.8% of the male students and 50.4% of the female students ate between meals 'once a day'. When questioned if they were satisfied with their body shape, 17.8 and 45.2% of the male students answered they were 'satisfied' or needed to 'gain weight', respectively, whereas 17.8 and 77.5% of the female students answered they were 'satisfied' or needed to 'lose weight', respectively. The results of the UCP2 gene polymorphism analysis showed that 33.7% of the males belonged to the DI heterozygote group, 64.2% belonged to the DD homozygote group, and 2.1% belonged to the II homozygote group. For the female students, 63.4% belonged to the DI heterozygote group, 35.1% belonged to the DD homozygote group, and 1.5% belonged to the II homozygote group. According to the blood and serum lipid analyses, the male students showed average HDL-cholesterol, LDL-cholesterol, and hemoglobin levels of 57.20, 93.80, and 15.00 mg/dL, respectively, while the female students presented average levels of 56.69, 102.88, and 13.13 mg/dL, respectively. In conclusion, this study found no significant effects in terms of UCP2 gene polymorphisms, but it is suggested that practical plans must be designed that allow college students to use nutritional knowledge in their daily lives, and in particular, nutrition education needs to be develop that would enable female college students to recognize their bodies appropriately and to control their weight in desirable ways.

Evaluation of Nonanchored Inter Simple Sequence Repeat (ISSR) Marker to Detect DNA Damage in Common Bean (Phaseolus vulgaris L.) Exposed to Acrylamide

  • Enan, Mohamed R.
    • Journal of Forest and Environmental Science
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    • 제24권2호
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    • pp.61-68
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    • 2008
  • Acrylamide is present as a contaminant in heated food products, predominantly from the precursor asparagine. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. In order to assess the feasibility of microsatellite primers as markers for DNA damage, the study was conducted on common bean (Phaseolus vulgaris L.) exposed to different concentrations of acrylamide. Polymorphisms were abundant among plant samples treated with acrylamide in comparison to control (untreated one) tested with 4- tri-nucleotide, 2 tetra-nucleotide, and 3- dinucelotide primers. The primer (CCG)4 was the best tested primer to generate polymorphism between the DNA of plants treated or not by acrylamide. Polymorphisms became evident as the presence and absence of DNA fragments in treated samples compared with the untreated one. The highest number of DNA variation on ISSR patterns was observed at the micromollar concentrations of acrylamide. Acrylamide was able to induce DNA damage in non concentration-dependent manner with effectiveness at micromollar concentrations. This study demonstrated that ISSR markers can be highly reliable for identification of DNA damage induced by acrylamide.

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RAPD 및 ITS 염기서열 분석을 이용한 곰취 속(Ligularia) 식물의 유연관계 분석 (Phylogenetic Relationship of Ligularia Species Based on RAPD and ITS Sequences Analyses)

  • 안순영;조광수;유기억;서종택
    • 원예과학기술지
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    • 제28권4호
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    • pp.638-647
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    • 2010
  • RAPD와 ITS 염기서열 분석을 통하여 $Ligularia$ 속 식물 5종류의 유연관계를 밝혔다. RAPD 분석에서는 총 196개의 random primer를 사용하여 밴드수가 많고 선명한 63개의 primer를 선발하였다. 다형성을 나타낸 밴드는 141개(31.8%)이었으며, 증폭된 크기는 0.2-1.6kb로 다양하였다. 유집 분석 결과, 유사도 값은 0.54-0.95의 범위로 나타났고, 0.77을 기준으로 크게 5그룹으로 나누었다. ITS 영역의 염기서열 분석 결과, ITS 1과 ITS 2 지역은 각각 248-256bp와, 220-222bp로 구성되어 있으며, 5.8S 부분은 164bp로 나타났다. ITS 1과 ITS 2 지역의 총 478개의 염기 중 49(10.2%)군데에서 변이가 있었으며, 구아닌(G)과 시토신(C)의 비율은 ITS 1 지역에서 49.4%, ITS 2에서는 53.5%로 나타났다. 염기서열 분석결과 5종류는 단계통을 형성하였으며, 갯취는 군외군으로 부터 가장 먼저 분계조를 형성하였다. 한대리곰취와 어리곤달비는 79%의 지지율을 가지고 유집되었으며, 곰취와 곤달비도 함께 유집되었지만 지지도는 52%로 낮았다. 이상의 결과에서 두 데이터는 일치하는 결과를 보였지만 한 대리 곰취의 분류학적 위치는 RAPD와 ITS 분석결과가 일치하지 않았다.

Bacterial Community and Biological Nitrate Removal: Comparisons of Autotrophic and Heterotrophic Reactors for Denitrification with Raw Sewage

  • Lee, Han-Woong;Park, Yong-Keun;Choi, Eui-So;Lee, Jin-Woo
    • Journal of Microbiology and Biotechnology
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    • 제18권11호
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    • pp.1826-1835
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    • 2008
  • An autotrophic denitrification reactor (ADR-l) and a heterotrophic denitrification reactor (HDR-2) were operated to remove nitrate and nitrite in an anoxic environment in raw sewage. The $NO_3$-N removal rate of ADR-l was shown to range from 52.8% to 78.7%, which was higher than the $NO_3$-N removal rate of HDR-2. Specific denitrification rates (SDNR) of ADR-l and HDR-2 were 3.0 to 4.0 and 1.1 to $1.2\;mgNO_3$-N/gVSS/h, respectively. From results of restriction fragment length polymorphism (RFLP) of the 16S rRNA gene, Aquaspirillum metamorphum, Alcaligenes defragrans, and Azoarcus sp. were $\beta$-Proteobacteria that are affiliated with denitritying bacteria in the ADR-l. Specifically, Thiobacillus denitrificans was detected as an autotrophic denitrification bacteria. In HDR-2, the $\beta$-Proteobacteria such as Denitritying-Fe-oxidizing bacteria, Alcaligenes defragrans, Acidovorax sp., Azoarcus denitrificans, and Aquaspirillum metamorphum were the main bacteria related to denitrifying bacteria. The $\beta$-and $\alpha$-Proteobacteria were the important bacterial groups in ADR-l, whereas the $\beta$-Proteobacteria were the main bacterial group in HDR-2 based on results of fluorescent in situ hybridization (FISH). The number of Thiobacillus denitrificans increased in ADR-l during the operation period but not in HRD-2. Overall, the data presented here demonstrate that many heterotrophic denitritying bacteria coexisted with autotrophic denitrifying bacteria such as Thiobacillus denitrificans for nitrate removal in ADR-l. On the other hand, only heterotrophic denitritying bacteria were identified as dominant bacterial groups in HDR-2. Our research may provide a foundation for the complete nitrate removal in raw sewage of low-COD concentration under anoxic condition without any external organic carbon or the requirement of post-treatment.

Evaluation and Selection of Potential Parents Based on Selection Indices and Isozyme Variability in Silkworm, Bombyx mori, L.

  • Moorthy S.M.;Das S.K.;Rao, P.R.T.;Urs S. Rao,;Sarkar A.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제14권1호
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    • pp.1-7
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    • 2007
  • In order to find out the appropriate parents for the breeding programme, twelve bivoltine and three multivoltine silkworm breeds were evaluated on the basis of multivariate selection index and isozyme analysis. Of which, four [CSR2, D6 (P), SK3, SK4] bivoltine and two multivoltine (Nistari, Cambodge) breeds were selected and breeding initiated to develop higher survival bivoltine silkworm breed suitable for tropical conditions. Among two isozyme (Esterase and acid phosphatase) analyzed, only esterase exhibited polymorphism among the bivoltine breeds. No polymorphism was observed among multivoltine in respect of esterase as well as acid phosphatase.

지속성 고빌리루빈혈증과 연관된 모유 황달에서 UGT1A1(Gly71Arg, TATA box) 다형성에 대한 연구 (The relationship between Gly71Arg and TATA box polymorphism of GT1A1 gene and prolonged hyperbilirubinemia of breast milk feeding infant in Korean)

  • 이재명;한영지;김지숙;김은령
    • Clinical and Experimental Pediatrics
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    • 제51권2호
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    • pp.150-155
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    • 2008
  • 목 적 : 모유 황달은 지속성 황달을 일으키는 중요한 원인이다. 모유 황달은 모유의 여러 가지 성분으로 인해 일어난다고 알려져 있지만 아직 그 원인이 명확하게 밝혀지지 않았으나 이전의 연구에서는 모유 황달이 가족력과 관계있다는 결과도 제시되었다. 이에 저자들은 신생아 황달을 가진 모유수유 환아들에서 Gly71Arg와 TATA box 유전자의 다형성을 조사한 후 이것이 한국인 신생아의 지속성 황달과 연관성이 있는지 알아보고자 본 연구를 시행하였다. 방 법 : 혈중 빌리루빈 수치가 10 mg/dL 이상의 건강하고 위험인자가 없는 모유수유를 시행한 만삭아 중 신생아 황달 환자 50명과 대조군 162명으로부터 혈액 0.5 cc를 채취하여 DNA를 분리하였고 TATA box와 Gly71Arg 유전자를 PCR 증폭하였다. 이를 염기서열 분석 방법을 통해 각 유전자의 다형성을 확인하였다. 결 과 : Gly71Arg 다형성은 환자군 50명 중 2명(4.0%)에서 AA로 40명(80.0%)은 GA로 나타났으며, 유전자가 분석된 대조군 129명 중 5명(3.9%)에서 AA로 4명(3.1%)은 GA로 나타났고, 대립유전자 빈도는 대조군에서 44.0% 환자군에서 5.5%로 나타났다(P=0.000001). TATA box는 환자군 50명 중 1명에서 $A(TA)_6TAA/A(TA)_7TAA$로 나타났으며 대조군에서 모두 $A(TA)_6TAA/A(TA)_6TAA$로 나타났다. 결 론 : 모유수유한 한국인 신생아 지속성 고빌리루빈혈증에서 UGT1A1의 Gly71Arg과 TATA box의 다형성을 확인하였으며, Gly71Arg 유전자의 다형성은 고빌리루빈혈증군에서 대조군에 비해 의미 있게 증가되어 모유수유한 한국인 신생아 지속성 고빌리루빈혈증과 연관이 있었고, TATA box의 다형성은 연관이 없었다.

한국산 가막사리속(Bidens L., 국화과) 수과 형태의 분류학적 검토 (The taxonomic consideration of achene morphology in Bidens L. (Asteraceae) in Korea)

  • 김선유;홍석표
    • 식물분류학회지
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    • 제38권4호
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    • pp.509-522
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    • 2008
  • The achene morphology of ten taxa (eight species and two varieties) of the genus Bidens L. (Asteraceae) in Korea was studied in detail using the stereo-microscopy (SM) and scanning electron microscopy (SEM). The size of achenes excluding the arista part is $3.0-19.5{\times}0.7-2.6 mm$. The pappus is made up of 2-4 aristae, and the length of arista is 2.2-5.6 mm long. The striations of barb are 1-3. The achene polymorphism is confirmed in six studied taxa (B. bipinnata, B. biternata, B. frondosa, B. parviflora, B. pilosa var. pilosa, B. tripartita var. tripartita). Four types of achene were recognized based on the shape and the number of arista per fruit. - Type A: oblong with two aristae (B. parviflora); Type B: oblong with three or more aristae (B. bipinnata, B. biternata, B. pilosa var. pilosa, B. pilosa var. minor); Type C: obovate with two aristae (B. frondosa, B. radiata var. radiata, B. radiata var. pinnatifida, B. tripartita var. tripartita); Type D: obovate with three or more aristae (B. cernua). Three surface patterns (i.e., spiral, smooth, and irregular straight in parallel) of barb are also distinguished. The achene surface can be recognized into three types (ribbed areolate, smooth, subfavulariate). Systematic implications of achene characters are also briefly discussed. On the basis of the achene characters, a key of Bidens taxa in Korea is provided.

홍띠 기내 재생과 재생 녹색식물체의 유전적 안정성 (In vitro Regeneration and Genetic Stability Analysis of the Regenerated Green Plants in Japanese Blood Grass (Imperata cylindrica 'Rubra'))

  • 강인진;이예진;배창휴
    • 한국자원식물학회지
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    • 제34권2호
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    • pp.156-165
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    • 2021
  • 바이오에너지작물의 중요한 소재를 제공하는 화본과 식물인 홍띠(Imperata cylindrica 'Rubra')의 기내재생 식물체의 유전적 안정성을 조사하고자 생장점 부위를 기내배양하여 재분화시킨 녹색 재생식물체의 변이성을 ISSR 마커로 조사하였다. MS(Murashige and Skoog, 1962) 배지에 식물체 기부의 생장점 부위를 적출하여 캘러스를 유도하고(0.1 mg/L 2,4-D와 2 mg/L BA), 캘러스 증식(0.1 mg/L 2,4-D와 0.05 mg/L BA), 신초 재분화(0.01 mg/L NAA와 2 mg/L BA) 후 MS 배지에서 발근시켜 재분화 식물체를 유도하고 100% 활착시켰다. 대조구인 모식물체 홍띠 8개체, 1년간 노지 포장에서 재배중인 재분화 녹색 식물체 10개체와 실험실 내 화분에서 재배중인 재분화 녹색 식물체 10개체, 총 28개체에 대하여 ISSR 분석한 결과 유전적 다형성은 재분화 식물체가 실내포트 재배식물체 4.1% 및 노지 재배식물체 3.1%로 0%인 대조구보다 높게 나타났다. 또한, 총 28개체들 간의 유전적 유사도를 평가한 결과, 유전적 유사도 지수는 0.919~1.0 사이에 분포하며, 평균 0.972로 유전적 충실도가 높게 나타났다. 군집분석 결과 노지에서 재배한 재분화 식물체와 모식물체(대조군)가 독립적으로 유집되었다.