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Optimal Larval Density and Low Temperature Storage Conditions for Rearing of Protaetia brevitarsis (Coleoptera: Cetoniidae) using a Fermented Mulberry Sawdust-base Diet (뽕나무발효톱밥을 이용한 흰점박이꽃무지(딱정벌레목: 꽃무지과) 유충 사육에서 적정 사육밀도 및 저온처리 조건)

  • Ju-Rak, Lim;Hyung-Cheol, Moon;Na-Young, Park;Sang-Sik, Lee;Woong, Kim;Chang-Hak, Choi;Hee-Jun, Kim
    • Korean journal of applied entomology
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    • v.61 no.4
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    • pp.555-562
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    • 2022
  • We raised the white-spotted flower chafer, Protaetia brevitarsis seulensis, from the larvae stage using a fermented mulberry sawdust-base diet at 25℃ and 16:8 h light:dark photoperiod. First, we determined the optimal density of the neonate larvae to be introduced into a rearing box (543 × 363 × 188 mm). The survival rates of the larvae were higher than 80% at 90 days after rearing at 100-175 larval densities but reduced by more than 10% at 200 larval density. The larval weights at 100 to 150 larval densities were similar; however, the weights at 175 and 200 larval densities were lower than those at 100 larval densities, indicating that the rate of weight gain increased under lower density. Based on these results, we inferred that 100-150 larvae was the optimal density. Second, we investigated the storage conditions of the last instar larvae under low temperatures. Four weight groups (1.8-2.0, 2.0-2.3, 2.3-2.5, and 2.5 g) of larvae were stored at 4, 8, and 10℃, respectively. All the larvae at 4℃ died 70 days after storage, whereas 80% of the larvae survived at 8℃ 70 days after storage, indicating that high larval weight was associated with high survival rates. The adults that emerged from larvae with more than 2.3 g and stored for up to 50 days at 4℃ laid few eggs. However, the adults that emerged from all larval weight groups stored for 70 days at 8℃ and 10℃ laid several eggs successfully. Based on these results, we inferred that the last instar larvae with more than 2.3 g could be stored for 30-50 days at 8℃.

Mapping the Research Landscape of Wastewater Treatment Wetlands: A Bibliometric Analysis and Comprehensive Review (폐수 처리 위한 습지의 연구 환경 매핑: 서지학적 분석 및 종합 검토)

  • C. C. Vispo;N. J. D. G. Reyes;H. S. Choi;M.S. Jeon;L. H. Kim
    • Journal of Wetlands Research
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    • v.25 no.2
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    • pp.145-158
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    • 2023
  • Constructed wetlands (CWs) are effective technologies for urban wastewater management, utilizing natural physico-chemical and biological processes to remove pollutants. This study employed a bibliometric analysis approach to investigate the progress and future research trends in the field of CWs. A comprehensive review of 100 most-recently published and open-access articles was performed to analyze the performance of CWs in treating wastewater. Spain, China, Italy, and the United States were among the most productive countries in terms of the number of published papers. The most frequently used keywords in publications include water quality (n=19), phytoremediation (n=13), stormwater (n=11), and phosphorus (n=11), suggesting that the efficiency of CWs in improving water quality and removal of nutrients were widely investigated. Among the different types of CWs reviewed, hybrid CWs exhibited the highest removal efficiencies for BOD (88.67%) and TSS (95.67%), whereas VSSF, and HSSF systems also showed high TSS removal efficiencies (83.25%, and 78.83% respectively). VSSF wetland displayed the highest COD removal efficiency (71.82%). Generally, physical processes (e.g., sedimentation, filtration, adsorption) and biological mechanisms (i.e., biodegradation) contributed to the high removal efficiency of TSS, BOD, and COD in CW systems. The hybrid CW system demonstrated highest TN removal efficiency (60.78%) by integrating multiple treatment processes, including aerobic and anaerobic conditions, various vegetation types, and different media configurations, which enhanced microbial activity and allowed for comprehensive nitrogen compound removal. The FWS system showed the highest TP removal efficiency (54.50%) due to combined process of settling sediment-bound phosphorus and plant uptake. Phragmites, Cyperus, Iris, and Typha were commonly used in CWs due to their superior phytoremediation capabilities. The study emphasized the potential of CWs as sustainable alternatives for wastewater management, particularly in urban areas.

Effect of Adding Milk on Compatibility with 3D Printing in the Preparation of a Surimi Mixture (수리미 혼합물 제조 시 우유 첨가에 따른 3D 프린팅 적합성에 미치는 영향)

  • Yoo-Seok Kang;Hye-ji Hwang;Ye-Lin Park;Hyeon-Su Han;Jeong-Cheol Park;Hun-Seo Seo;Ye-Hui Choi;Su-Hyeong Kim;Ka-Eun Woo;So-Mi Jeong;Ga-Hye lee;Dong-Hyun Ahn
    • Journal of Life Science
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    • v.33 no.5
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    • pp.391-396
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    • 2023
  • Milk is an emulsion, improving texture of surimi mixture and able to suppress off flavors and abnormal tastes. Therefore, this study aimed to identify the effective properties of milk in the preparation of a surimi mixture for 3D printing. The sensory and physical properties of surimi mixtures containing 0%, 20 wt%, and 40 wt% milk were evaluated, where the unheated surimi mixture with added milk demonstrated increased firmness and adhesiveness compared to the negative control group. In addition, the hardness, adhesiveness, gumminess, and chewiness of the mixture containing 40% milk were highest, but springiness, cohesiveness, and resilience were lowest. In the sensory evaluation, as the amount of milk increased, a fishy smell, abnormal taste and texture improved, hardness and preference increased as well. From these results, it was confirmed that a surimi mixture can be prepared with milk to improve its physical and sensory properties for 3D printing when compared to the negative control mixture. In particular, it was revealed that the physical properties and preference of the surimi mixture are best when prepared with 40% milk.

Improving the Shelf Life of Pork by using a Coating Agent with Mandarin Peel Powder (귤 과피 분말을 첨가한 피막제 처리에 의한 돈육의 저장성 향상)

  • Hyeonjeong Choi;Yong-Suk Kim
    • Journal of Food Hygiene and Safety
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    • v.38 no.2
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    • pp.55-62
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    • 2023
  • This study aimed to investigate the effect of a coating agent on pork storage. Pork was coated with a coating agent containing sodium carboxymethyl cellulose (CMC) and mandarin peel powder (M). The treatments were divided into control, a 0.1% CMC treatment, and a 0.1% CMC +5% M treatment, and pH, color, 2-thiobarbituric acid reactive substances (TBARS), volatile basic nitrogen (VBN), and the number of viable cell counts were measured. In the case of redness (a), it was found that the reduction over the storage period was less in the 0.1% CMC + 5% M treatment than in the control and the 1% CMC treatment. When stored at 4℃ and 25℃, TBARS of pork tended to increase during the storage period, followed by control, 0.1% CMC treatment, and 0.1% CMC + 5% M treatment, indicating that lipid oxidation was most suppressed in pork coated with mandarin peel powder. As a result of measuring the VBN of pork stored at 4℃ and 25℃, the 0.1% CMC + 5% M treatment showed lower values than the control and 0.1% CMC treatment. When the film-coated pork was stored at 4℃, the number of viable cell counts in the 0.1% CMC +5% M treatment area was 7.13±0.96 log CFU/g on the 12th day of storage, delaying the growth of viable cell counts for approximately 3 d more than other treatments. Therefore, coating pork with a film containing CMC and mandarin peel powder has been confirmed to delay the increase in the number of viable cell counts while reducing the quality change during pork storage, which is an effective alternative to improving the storage of fresh food as an edible film.

Quality Changes of 'Baumkuchen' Cake with Modified Atmosphere Packaging during Storage (변형기체포장 처리에 따른 '바움쿠헨' 케이크의 저장 중 품질 특성 변화)

  • Myungho Lee;Minhwi Kim;Youn Suk Lee
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.29 no.2
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    • pp.87-94
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    • 2023
  • Fresh bakery products are widely consumed worldwide and therefore particular requirements for their quality characteristics have been established. The shelf life of bakery products is mainly subjected to microbial spoilage and staling. This study investigated the optimum conditions of modified atmosphere packaging (MAP) application to extend the shelf life of the bakery products. The gas conditions of the headspace in 'Baumkuchen' cake were 0, 30, 70, and 100% CO2 concentrations and stored at 30℃ for 5 days. The bakery samples were evaluated weight loss, hardness, color change, pH and total aerobic bacteria, yeast and molds count throughout the storage period. Values of the weight loss and hardness were increased over the storage period, meanwhile pH was significantly decreased. However, no significant color changes were observed during storage. It was also found no significant difference between the different gas treatments. Total aerobic bacteria count of the stored samples after day 5 was increased by 6.94 log CFU/g in the air filled package, compared to 6.20 log CFU/g in the 100% CO2 filled package and 6.02 log CFU/g in the 70% CO2 filled package. Yeast and molds count were 3.65 log CFU/g in air filled package, 2.66 log CFU/g in 100% CO2 filled package, 2.64 log CFU/g in 70% CO2 filled package, 2.86 log CFU/g in 30% CO2 filled package and 3.31 log CFU/g in 100% N2 filled package on day 2. In conclusion, it was shown that 70% and 100% CO2 treatments in the package were effective to reduce microbial growth.

Fertigation Techniques Using Fertilizers with Peristaltic Hose Pump for Hydroponics (연동펌프를 이용한 비료염 공급 관비재배기술 연구)

  • Kim, D.E.;Lee, G.I.;Kim, H.H.;Woo, Y.H.;Lee, W.Y.;Kang, I.C.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.17 no.1
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    • pp.57-71
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    • 2015
  • This study was conducted to develop the fertigation system with a peristaltic hose pump and brushless DC motor. The fertigation system was consisted of sensor, main controller, motor control unit, peristaltic pump, water supply pump, control panel, and filter. The peristaltic pump discharges liquid by squeezing the tube with rollers. Rollers attached to the external circumference of the rotor compresses the flexible tube. The fluid is contained within a flexible tube fitted inside a circular pump casing. The developed fertigation system has no mixing tank but instead injects directly a concentrated nutrient solution into a water supply pipe. The revolution speed of the peristaltic pump is controlled by PWM (Pulse width modulation) method. When the revolution speed of the peristaltic pump was 300rpm, the flow rate of the 3.2, 4.8, 6.3mm diameter tube was 202, 530, 857mL/min, respectively. As increasing revolution speed, the flow rate of the peristaltic pump linearly increased. As the inner diameter of a tube larger, a slope of graph is more steep. Flow rate of three roller was more than that of four roller. Flow rate of a norprene tube with good restoring force was more than that of a pharmed tube. As EC sensor probe was installed in direct piping in comparison with bypass piping showed good performance. After starting the system, it took 16~17 seconds to stabilize EC. The maximum value of EC was 1.44~1.7dS/m at a setting value of 1.4dS/m. The developed fertigation system showed ±0.06dS/m deviation from the setting value of EC. In field test, Cucumber plants generally showed good growth. From these findings, this fertigation system can be appropriately suitable for fertigation culture for crops.

Development of cordycepin fortified milk production in Holstein cows I. Effects of various levels of Cordyceps militaris mycelia from grains supplement on cordycepin content in milk in dairy cows (Cordycepin 강화 우유 생산에 관한 연구 I. 동충하초 균사체의 적정사용량 결정을 위한 사양연구)

  • Yeo, J.M.;Lee, S.H.;Kim, D.H.;Hwang, J.H.;Kim, W.Y.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.11 no.1
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    • pp.103-111
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    • 2009
  • This study was carried out to determine effects of long-term supply of Cordyceps militaris mycelia on cordycepin content in milk in dairy cows. Ten Holstein cows in the early stages of lactation were divided into two groups. Control group received no supplement whereas treatment group received 6% of C. militaris mycelia of their dry matter intake for 6 months. Feed intake, milk urea nitrogen and somatic cell counts were not affected by long-term supply of C. militaris mycelia for the whole period. In addition, milk yield and milk composition were not affected by long-term supply of C. militaris mycelia at any time of the periods with the exception of milk protein content and yield. The average of milk protein content and yield from the whole period was higher for C. militaris mycelia supplement group than for the control group. As expected, cordycepin in whole blood and milk was not detected in the control group. The range of cordycepin content in the treatment was 0.31~0.38µ/ml and 0.18~0.26(µ/ml for whole blood and milk, respectively. Individual variation was found to be very high and, furthermore cordycepin was undetected in some milk samples. Thus, no clear pattern could be seen in cordycepin content in milk throughout the whole period. Overall, the results of the present study suggest that the transfer efficiency of cordycepin to milk by supplementing C. militaris mycelia in dairy cows was unpredictable and low.

Incidence of Fusarium Wilt of Sesame (Sesamum indicum L.) in Relation to Air Temperature (참깨 시들음병(病) 발병(發病)과 재배기간중(栽培期間中) 온도(溫度)와의 관계(關係))

  • KANG, S.W.;CHO, D.J.;Lee, Y.S.
    • Korean journal of applied entomology
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    • v.24 no.3 s.64
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    • pp.123-127
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    • 1985
  • Incidence of the Fusarium wilt caused by F. oxysporum f. sp. vasinfectum of sesame (var. Kwangsan) was remarkably influenced by seeding date and mean air temperature in the field of two or three year's continuous cropping with sesame in 1983 and 1985. Sesame were seeded on six different dates from April 20 to July 5. Air temperature was checked daily at the meteorological station near the experimental field. Low($16{\sim}20^{\circ}C$) and high temperature($20{\sim}25^{\circ}C$) periods were provisionally devided, based on every ten-day mean daily temperature during field experiment for last ten years, which corresponded to before and after June 15 in Jinju, Gyeong-nam. Infection rates were 83.7%, 68.2% and 59.4% in the plants grown for 55 days (seeding date: April 20), 40 days(May 5) and 25 days(May 20) under low temperature. On the other hand, infection rates were below 3% in those plots seeded during high temperature period. The longer the growth period exposed to low temperature, the higher was infection rates. It is interesting to note that 40 days old seedling or older are prone to severe infection compared to the younger ones, in higher temperature of $20{\sim}25^{\circ}C$. Therefore, seedlings in vegetative growth stage are less prone to infection than these in reproductive growth stage. The result showed that air temperature during sesame growth was one of the most important factor affecting the incidence of Fusarium wilt. This suggested that sesame crop, which is of tropical origin, has been predisposed to Fusarium wilt, when the plants were exposed to low temperature of $16{\sim}20^{\circ}C$.

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Studies on Genetice of Blast Resistance in Rice L Inheritance of Resistance to Specific Races of Blast Fungus and Relationship between Their Resistance and II, VIII, XI and XII Linkage Groups in Some Rice Varieties (수도품종의 도열병 저항성 유전분석 제1보 특정 도열병 균계에 대한 저항성 품종들의 저항성 유전분리와 II, VIII, XI 및 XII번 연관과의 관계)

  • Chae, Y.A.;Park, S.Z.;Ha, S.B.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.1
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    • pp.32-39
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    • 1981
  • In order to study the genetic system of the blast resistant varieties, the conidial suspension of mutant races of T-2$^{+t}$, N-2$^{+t}$, C-8$^{+t}$ was inoculated at 4-5 leaf stage by injector for F_2 seedlings from the crosses between seven resistant varieties and four maker lines easily detectable at seedling stage. The results are summarized as follows; 1. The fertility of cross between Semi-dwaf testers and Indica resistant varieties except Carreon was about 74 percents. 2. The segregation modes of resistance varied with varieties and blast races. However, the resistance was expressed as dominance in all cases. Tetep, Tadukan and Carreon showed more complicated segregation for resistance than that of the bred lines. 3. For blast races used, four segregation ratios such as 3:1, 9:7, 13:3 and 37:27 were found in the Tatukan, Tetep, and IR747, and three segregation ratios such as 3:1, 13:3 and 15:1 in the Carreon, and two segregation ratios of 3:1 and 13:3 with Suweon 287, Suweon 288, and Iri342. 4. In the segregation of the resistance to the each races, the ratios of 3:1, 13:3, 15:1 were fitted to T-2$^{+t}$, and the ratios of 3:1, 13:3, 9:7 and 37:27 to N-2$^{+t}$ and C-8$^{+t}$. 5. Suweon 287, Suweon 288 and Iri342 carried one simple dominant gene and inhibitor gene was considered in some cross combinations. Meanwhile Tadukan, Tetep and IR747 seemed to carry one to three resistant genes, and in some cross combinations, the expression of these genes were simple dominant, inhibiting, duplicating and complimentary action. 6. Resistance genes to blast races, T-2$^{+t}$, N-2$^{+t}$ and C-8$^{+t}$ in the Tadukan, Tetep, Carreon, Suweon 287, Suweon 288 and Iri342 were found to be independent with the linkage group of II(lg), VIII(la), XI(bc), and XII(gl).bc), and XII(gl).

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Relative Toxicity of Abamectin to the redatoryMite Amblyseius womersleyi Schicha (Acari: Phytoseiidae) and Twospotted Spider MIte Tetranychus urticae Koch (Acari: Tetranychidae) (아바멕틴의 긴털이리응애(Amblyseius womersleyi Schicha)와 점박이응애(Tetranychus urticae Koch)에 대한 선택독성)

  • Park, C.G.;Lee, M.H.;Yoo, J.K.;Lee, J.O.;Choi, B.R.
    • Korean journal of applied entomology
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    • v.34 no.4
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    • pp.360-367
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    • 1995
  • The relative toxicity of abamectin was assessed to the predatory mite Amblyseius womersleyi Schicha and to dicofol-resistant and -susceptible twospotted spider mite (TSM) Tetranychus urticae Koch in the laboratory. Abamectin was much les toxic to the predator than to the spider mite. At 0.12 and 0.6 ppm, all TSM adult females of the tow strains were killed within 48 h after dipping n the solutions. The lower concentrations (0.06 and 0.012 ppm) killed more than 77% of TSM female adults of the two strains at 120 h after treatment. However, abmectin did not significantly affect the survival and mobility of A. womersleyi female adults at a concentration of 0.12 ppm but the mortality was slightly increased up to 20~23% at 0.6 and 6 ppm. Abamectin did not significantly affect hatchability of one-day old TSM eggs at 0.06~0.6 ppm. The Four-day old eggs were much more susceptible to abamectin than one-day old eggs were. Within 0.006-6 ppm, abamectin did not affect the hatchability of A. womersleyi eggs and the development of resulting immature predators. When the predator female adults were dipped in 0.6 and 0.12 ppm solution, their reproduction was not affected, but at 6 ppm it was decreased by 35%. However, the reproduction of TSM reduced significantly at concentrations between 0.006 and 0.6 ppm. The differential toxicity of abamectin between TSM and the predator could be of practical importance in managing spider mite populations in the field. Abamectin at selective sublethal concentrations (i.e., 0.012~0.06 ppm) could be of value in adjusting predator/prey ratios in integrated management of spider mites.

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