• Journal of Mushroom
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• v.9 no.2
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• pp.69-73
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• 2011

We have researched on methods which can reduce fruit-body malformations of king oyster mushroom(Pleurotus eryngii). We collected many pathogens from diseased fruit-body or malformated fruit-body and identified with chemicobiological test and microscope. The factors of fruit-body malformations and increment of contamination during the pin-heading induction time were researched with ventilation amounts in growing room. When the pathogens having high pathogenicity were inoculated in spawn running bottle and at pin-heading induction time, symptom appeared or not appeared in according to air ventilation amounts in growing room. During the pin-heading induction time, humidity degree in growing room have kept of high level and air ventilation amounts were so little that fruit-body malformations ratio was high. But, even though pathogens were inoculated at the surface of bottle factitiously, if air ventilation amounts were enough, fruit-body malformation ratio was low.

• Journal of Mushroom
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• v.16 no.3
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• pp.140-146
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• 2018

Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at $4^{\circ}C$. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.

• Mycobiology
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• v.41 no.4
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• pp.252-255
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• 2013

Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.

• Journal of Mushroom
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• v.3 no.3
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• pp.95-99
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• 2005

Carbon dioxide($CO_2$) promotes development of primordia and depress differentiation of sporophore and gill containing basidia, have an effect on quality. This experiments were conducted to elucidate optimal level of $CO_2$ for king oyster mushroom(Pleurotus eryngii). King oyster mushroom was cultivated under 1,600, 2,400, and 3,200ppm $CO_2$. Harvest ratio in normal plot were 98.6, 99.3, and 93.8% at 1,600, 2,400, 3,200ppm, respectively, so 2,400ppm is optimal. The yield per bottle was 102.5g at 2,400ppm, better than 99.7g at 1,600ppm, The $CO_2$ concentration of 2,400ppm was also the best condition for quality, 6.1 at 2,400ppm was 115% of 1,600ppm's. In thinning treatment plot, quality at 2,400ppm was 9.5 better than 1,600ppm whose quality was 9.4. The yields per bottle were 90.7, 98.2, 77.3g at 1,600, 2,400, 3,200ppm respectively. These results show that 2,400ppm was optimal $CO_2$ concentration for quantity of King Oyster mushroom as well as quality.

• Journal of Mushroom
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• v.10 no.4
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• pp.198-202
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• 2012

Recently, Pleurotus eryngii, King oyster mushroom, is main item of exportation in South Korea. For safe transportation to Europe and USA, it is necessary to elucidate suitable conditions of storage and treatment of post-harvest during long shipping period. For the selection of suitable package type, P. eryngii were stored at $0^{\circ}C$ and $4^{\circ}C$ after trimming and non-trimming package. Whole mushroom were packed with polypropylene anti-fog film about 400g. The weight loss ratio and elongation of pileus were increased with increasing storage time and temperature. There were little differences between trimming and non-trimming type in freshness at low temperature storage, $0^{\circ}C$ and $4^{\circ}C$. However, the freshness of non-trimming type P. eryngii at room temperature after low temperature storage is longer than that of trimming type. It was found that the optimum storage temperature and post-harvest treatment of P. eryngii were $4^{\circ}C$ and non-trimming type, respectively.

• Journal of Mushroom
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• v.16 no.3
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• pp.131-139
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• 2018

The king oyster mushroom (Pleurotus eryngii) is widely consumed because of its flavor, texture, and its functional properties such as antioxidant activity and prebiotic effects. However, long-term product storage and transportation (e.g., export) are difficult because of its limited durability. The shelf-life of king oyster mushroom is affected by environmental factors such as temperature, humidity, gas composition, and ventilation, which may affect sensory characteristics including respiration rate, texture, moisture, flavor, color, and pH. The major problems regarding storage of mushrooms are browning, flavor changes, and softening. To address these problems, novel preservation techniques were developed, and more durable variants were bred. Different drying methods, gamma irradiation, chitosan coating, modified atmosphere (MA) packaging, and controlled atmosphere (CA) storage were evaluated in order to extend the shelf-life of king oyster mushrooms. Freeze drying showed better results for the preservation of mushrooms than other drying methods. Irradiation with 1 kGy was more effective for extending mushroom shelf-life than higher doses. The preservative performance of chitosan-based films was improved by combining the compound with other hydrocolloids, such as oil, protocatechuic acid, and wax. The CA storage conditions recommended for king oyster mushrooms are 5kPa $O_2$ and 10 to 15kPa $CO_2$ at temperatures below $10^{\circ}C$. Active MA packaging with microperforated PP film was also effective for maintaining quality during storage.

• Journal of Mushroom
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• v.4 no.4
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• pp.135-143
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• 2006

We have investigated cultural circumstance and given condition of king oyster mushroom(Pleurotus eryngii) growing farmer. We collected many pathogens from King oyster mushroom growing farmer and identified with chemicobiological test and microscope. Most of investigated farmers neglected their's growing room cleaning and washing, after harvesting At pin-heading induction time, humidity degree in growing room was kept of high level and Air ventilation volume was so little that fruit-body formation ratio was low. The collected pathogens were twenty eight strains and identified with Pseudomonas sp., Trichoderma sp. mostly. During the spawn running time and pin-heading induction time, contamination by Trichoderma sp. occurred mostly, but during the fruit-body growing time, contamination by Pseudomonas sp., Erwinia sp. etc, occurred.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1836-1844
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• 2016

Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract of Pleurotus eryngii var. ferulae 'Beesan No. 2' (CEBT) on adipogenesis in 3T3-L1 murine preadipocytes. Pleurotus eryngii var. ferulae is one of many varieties of King oyster mushroom and has been reported to have various biological activities, including antitumor and anti-inflammation effects. Biological activities of 'Beesan No. 2', a new cultivar of Pleurotus eryngii var. ferulae, have not yet been reported. In this study, we found that CEBT suppressed adipogenesis in 3T3-L1 cells through inhibition of key adipogenic transcription factors, such as peroxisome proliferatoractivated receptor ${\gamma}$ and CCAAT/enhancer binding protein ${\alpha}$. Additionally, CEBT reduced the expression of the IRS/PI3K/Akt signaling pathway and its downstream factors, including mammalian target of rapamycin and p70S6 kinase, which stimulate adipogenesis. Furthermore, ${\beta}-catenin$, a suppressor of adipogenesis, was increased in CEBT-treated cells. These results indicate that Pleurotus eryngii var. ferulae 'Beesan No. 2' effectively inhibited adipogenesis, so this mushroom has potential as an anti-obesity food and drug.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2002.10a
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• pp.121-124
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• 2002

King Oyster(Pleurotus eryngii) is one of the most promising mushrooms produced on the domestic farms. The quality as well as quantity of King oyster is sensitively affected by micro climate factors such as temperature, relative humidity, $CO_2$ concentration, and light intensity. To safely produce high-quality King oysters year round, it is required that the environmental factors be carefully controlled by well designed structures equipped with various facilities and control systems. In this study, we are focusing on carrying out growing experiment to find out reasonable range of each environmental factor together with economic and safe structures influencing on the optimal productivity of king oyster mushroom. The optimal productivity will be evaluated by considering the quality and quantity of mushroom production, energy requirements, facility construction and management cost, etc.

• Journal of Mushroom
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• v.10 no.4
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• pp.191-197
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• 2012

This study was carried out to investigate proper modified atmosphere condition of king oyster mushroom (Pleurotus eryngii) during cold storage and distribution. King oyster mushrooms were divided into two treatments: $1{\mu}L/L$ 1-methylcyclopropene for 20 hours at $0^{\circ}C$ (1-MCP), while the other treatment was left at $0^{\circ}C$ (control). They were packaged with $30{\mu}m$ oriented polypropylene (OPP, $1238.0cc/m^2{\cdot}day{\cdot}atm\;O_2$), and microperforated (MP3, $3179.9cc/m^2{\cdot}day{\cdot}atm\;O_2$) film. Quality and sensory evaluation parameters of weight loss, stem firmness, skin color, off-flavor, overall quality were monitored after 3, and 4 weeks storage at $0^{\circ}C$ and plus 3 days on the shelf at room temperature. 1-MCP treated mushroom packaged with MP3 film kept 3.6~10.9% $O_2$ and 9.0~13.3% $CO_2$ concentration in the bag during storage, and showed high overall quality at 4 weeks storage at $0^{\circ}C$ + 3 days on the shelf at room temperature because of the lowest development of off-flavor, stem discoloration, and cap softening among the treatments.