Mycobiology

The Korean Society of Mycology (한국균학회)
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Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers

  • Kim, Sang-Woo (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Kim, Sinil (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Lee, Hyun-Jun (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Park, Ju-Wan (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
  • Received : 2013.09.16
  • Accepted : 2013.11.17
  • Published : 2013.12.31
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Abstract

Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.

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References

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