• Title/Summary/Keyword: kinetin,P

Search Result 81, Processing Time 0.023 seconds

Effects of Foliar Spray of Kinetin(6-Furfurylaminopurine) on the Growth of Mulberry Tree and the Metabolic Activities in the Leaves (Kinetin(6-Furfurylaminopurine)의 엽면살포가 상수의 생장 및 생체내 대사에 미치는 영향)

  • 이재하
    • Journal of Sericultural and Entomological Science
    • /
    • v.26 no.1
    • /
    • pp.1-20
    • /
    • 1984
  • Effects of Foliar spray of Kinetin on the growth and metabolism of mulberry tree was investigated in this studies. The results are summarized as follows; 1. Appropriate level of Kinetin for the optimal growth of mulberry tree was 100ppm. 2. In the non-fertilizer, N, P, K, and N+P+K treated plots supplemented with Kinetin, the growth of mulberry tree was generally promoted, especially in the roots, in comparison to non-supplemented plots. The effect was notably outstanding in the N, and N+P+K treated plots. 3. The mechanism by which the growth of root is stimulated was fundamentally attributed to the hypertrophy of unit cells. 4. The chlorophyll contents of the leaves in the Kinetin supplemented plots were higher than that of the non-supplemented, especially in the N, and N+P+K treated ones. 5. Likewise, total sugar contents of Kinetin supplemented plots were higher than that of the non-supplemented. Particularly the N+P+K treated plots showed higher level of sugar contents. In other plots, there were no significant differences in the level of sugar contents. 6. The activity of GOT and GPT was higher in the Kinetin supplemented plots, particularly in the N, and N+P+K treateated. 7. The contents of ascorbic acid were increased in plots with kinetin supplement in the order of N+P+K>potassium>Nitrogen>phosphorus$\geq$non-fertilizer. 8. Difference between Kinetin treatment and non-treatment was not recognized in the contents of inorganic and organic compounds.

  • PDF

Influence of Arbuscular Mycorrhizal Fungus and Kinetin on the Response of Mungbean Plants to Irrigation by Seawater

  • Rabie, G.H.
    • Mycobiology
    • /
    • v.32 no.2
    • /
    • pp.79-87
    • /
    • 2004
  • An experiment was carried out to investigate the effects of pre-inoculation with the mycorrhizal fungus Glomus clarum and foliar application of kinetin on the growth of mungbean plant irrigated wht different dilution of seawater. Arbuscular-mycorrhizal(AM) infection significantly increased dry weight, height, chlorophyll, sugar and protein content, nitrogen and phosphorus-use efficiencies, leaf conductivity, transpiration rate, nitrogenase, acid and alkaline phosphates activities of all salinized mungbean plants in comparison with control and non-mycorrhizal plants irrespective of the presence or absence of kinetin. Mycorrrhizal plants showed higher concentrations of N, P, K, Ca and Mg and lower Na/N, Na/P, Na/K, Na/Ca and Na/Mg ratios than non-mycorrhizal plants when irrigated with certain dilution of seawater. Mungbean plants showed 597% and 770% dependency on AM fungus G. clarum in absence and presence of kinetin, respectively, for biomass production under a level of 30% of seawater. The average value of tolerance index for mycorrhizal plants accounted 267% and 364% in absence and presence kinetin respectively. This study provides evidence for the benefits of kinetin which are actually known for mycorrhizal than non-mycorrhzal plants. AM fungus and kinetin protected the host plants against the detrimental effects of salt. However, mycorrhizal infection was much more effective than kinetin applications. Thus management applications of this arbuscular mycorrhizal endophyte(G. clarum) with kinetin could be of importance in using seawater in certain dilution for irrigation in agriculture.

Effect of Kinetin on In Vitro Development of Parthenogenetic Porcine Oocytes Exposed to Demecolcine Prior to Activation

  • Kim, Ki-Young;Park, Sang-Kyu;Roh, Sang-Ho
    • Journal of Embryo Transfer
    • /
    • v.24 no.2
    • /
    • pp.105-108
    • /
    • 2009
  • This study was designed to investigate the effect of kinetin on in vitro development of parthenogenetic porcine oocytes exposed to demecolcine prior to activation. In vitro matured metaphase II stage oocytes were incubated in 0 or 2 ${\mu}$g/ml demecolcine supplemented defined culture medium for 3 h and the oocytes were activated electrically. The parthenogenetic porcine embryos were then cultured in 0 or 200 ${\mu}$M kinetin supplemented defined culture medium for 7 days. Regardless of demecolcine treatment, kinetin supplementation increased blastocyst rates significantly (7.0% versus 12.1% and 4.9% versus 8.5%; Control versus Kinetin and Demecolcine versus Kinetin + Demecolcine, respectively, p<0.05). Demecolcine treatment before activation tended to decrease blastocyst rates regardless of kinetin supplementation although it is not statistically significant. Total cell numbers in the blastocysts also tended to be elevated in embryos when supplemented with kinetin, however only the result between Kinetin and Demecolcine groups is statistically significant (37.6 ${\times}$ 7.2 versus 28.1 ${\times}$ 9.5, respectively, p<0.05). In conclusion, the present report shows that kinetin enhances developmental competence of parthenogenetic porcine embryo regardless of demecolcine pre-treatment before parthenogenetic activation when they were developed in defined culture condition.

In Vitro Shoot Tip Culture of Haemaria discolor (Haemaria discolor 경정의 기내배양)

  • 왕영조;정재동;최수옥;지선옥
    • Korean Journal of Plant Tissue Culture
    • /
    • v.21 no.4
    • /
    • pp.227-231
    • /
    • 1994
  • The experiments were conducted to obtain culture conditions on optimal cultural conditions for propagation of a topical orchid, Haemaria discolor, through shoot tip cultrue in vitro. Survival percentage of shoot tip explant, in initial culture media was higher in H$_3$P$_4$ medium than in Murashige and Skoog's medium. Explants, cultured in H$_3$P$_4$ medium containing 1.0 mg/L kinetin, showed more shoot elongation when compared with those on other media. When the distal part of stem (pseudobulb) was longitudinally sectioned into half and cultured in H3P4 medium containing 0.1 mg/L 2ip, 0.1, or 1.0 mg/L kinetin, shoots from axillary bud were much more elongated under light condition.

  • PDF

Detection of flavonoid compounds by cell culture of Ginkgo biloba L (은행(Ginkgo biloba L.)의 세포배양에 의한 Flavonoid류의 검출)

  • 김광수;백윤웅
    • KSBB Journal
    • /
    • v.11 no.1
    • /
    • pp.1-7
    • /
    • 1996
  • Calli induced from Ginkgo bilha L. were cultured to investigate optimal culture conditions and identify the possibility production of useful compounds. Calli were obtained from leaves and stems of Ginkgo biloba seedlings and embryos on WP medium supplemented with 2mg/$\ell$ NAA and 5mg/$\ell$ kinetin. Chlorophyll-ricked green callus was inducted in MS liquid medium containing 1mg/$\ell$ NAA and 0.1mg/$\ell$ kinetin under light as 3 clones selected with origin. Embryo derived callus showed the highest growth rate. Analysis for flavonoids and their precursor was performed by TLC and EMS. A specific precursor of flavonoid was identified in callus, not in natural leaves. These findings indicate that tissue culture may produce rlavonoids.

  • PDF

Correlation between in vitro Flowering Frequency and the Structure of Cytokinins in Ginseng (Panax ginseng C.A. Meyer) (인삼 (Panax ginseng C.A. Meyer)의 기내 화아형성 빈도와 cytokinin 구조와의 관계)

  • 이행순;김윤성;권석윤;곽상수;유장렬
    • Korean Journal of Plant Tissue Culture
    • /
    • v.26 no.2
    • /
    • pp.109-113
    • /
    • 1999
  • To elucidate the relationship between in vitro flowering and the structure of cytokinins in ginseng (Panax ginseng C.A. Meyer), zygotic embryos, seedlings, and cotyledonary nodes were cultured on MS medium supplemented with 5 $\mu$M of various cytokinins (BA, kinetin, 2-iP, and zeatin) with or without GA$_3$ (5 $\mu$M). The frequency of in vitro flowering was the highest when explants were cultured on the medium containing BA regardless of the kinds of explants, followed by kinetin, 2-iP, and zeatin. Flowering frequency of cotyledonary node explants was significantly increased by the combined treatment of cytokinin and GA$_3$. Flowering frequency was highly correlated with the logP of cytokinins, indicating that the lipophilicity of each cytokinin may involved in the in vitro flowering of ginseng.

  • PDF

Studies on the Mass Propagation of Pinellia ternata (Thunb.) Breit in Vitro (조직배양에 의한 반하〔Pinellia ternata(Thunb.) Briet〕의 대량번식에 관한 연구)

  • 최정식;나의식
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.31 no.1
    • /
    • pp.30-42
    • /
    • 1986
  • In order to find out the best media, explants and environmental conditions for induction of calluses and organogeneses of Pinellia ternata (Thunb.) Breit in vitro, various parts of adult have been cultured on Murashige & Skoog's medium containing various levels of 2,4-dichlorophenoxy acetic acid(2,4-D) and kinetin. The results obtained were as follows: Calluses were induced from the surface of apical meristem and leaf tissue. Formation and growth of calluses in petiole ex plants were best on the MS medium complemented with 2,4-D 2.0 mg/l and kinetin 0.2mg/l. But callus formation in stem ex plants of the nearest tuber was not induced at all kinds of media. Plantlets occured at all treatment except absence of growth regulator. Their numbers, size, leaf and fresh weight were promoted by 2,4-D 2.0mg/l and kinetin 0.2mg/l. Root growth was increased on the medium containing higher 2,4-D concentrations. Size and fresh weight of callus were increased at 25$^{\circ}C$ compared with 10, 20 and 30$^{\circ}C$, respectively. Optimal pH value was at 6.0 for growth of callus. Morphological aberrations were observed in plantlets, especially in regenerated leaves. The separation of the broad leaved plantlets and albino were observed in some cultures. Growth of plantlets after transplantation was best in pots with the sterilized vermiculte. But abnormal variants withered up.

  • PDF

The Effect of Pyroligneous Liquor and Coconut Water on Plantlet Multiplication and in Vitro Flowering of Dendrobium moniliforme (목초액 및 코코넛액이 석곡(Dendrobium moniliforme)의 유묘 증식과 기내 개화에 미치는 영향)

  • Jee, Sun-Ok;Cho, Dong-Hoon
    • Journal of Life Science
    • /
    • v.15 no.5 s.72
    • /
    • pp.739-742
    • /
    • 2005
  • This experiment was carried out to clarify the effect of pyroligneous liquor and coconut water on plantlet multiplication and in vitro flowering of Dendrobium moniliforme. Plantlet growth and multiplication was good in 1.0 ml/L pyroligneous liquor treatment which was added to the basal media of 3 g/L hyponex and 4 g/L peptone ($H_{3} P_{4}$) containing 0.1 mg/L NAA and 1.0 mg/L kinetin. In the treatment of 30 ml/L coconut water showed good results on plantlet growth and multiplication. In vitro flowering showed highest rate in the treatment of 1.0 ml/L pyroligneous liquor and 30 ml/L coconut water which were added to the basal media of $H_{3} P_{4}$ containing 0.1 mg/L NAA and 1.0 mg/L kinetin.

Effect of Genotypes and Cytokinins on Shoot Multiplication of Cut Roses by Nodal Culture (절화 장미의 마디 배양시 품종과 사이토키닌류가 신초의 대량증식에 미치는 영향)

  • 박미혜;한경철;김경재;이동우
    • Korean Journal of Plant Tissue Culture
    • /
    • v.22 no.4
    • /
    • pp.217-221
    • /
    • 1995
  • This experiment was carried out to determine the effects of genotyes (cultivars) and kinds of cytokinins and concentrations on shoot multiplication of roses (Rosa hybrida) by nodal culture. The results from this study were summarized as follows. Shoot multiplication was significantly affected by genotypes. Most shoots were produced from 'Carl Red 'rather than two other cultivars, 'Golden Emblem' and 'Tineke'. Among 3 cytokinins, BA was more significantly effective for multiplication than two other cytokinins, 2iP and kinetin. However subsequent shoot growth after multiplication was more enhanced by 2iP and kinetin rather than BA. An optimal concentration of cytokinin for shoot multiplication was 1 mg/L in this study but BA at 1-5 mg/L was considered optimal for multiplication ratio itself. Subsequent shoot growth was significantly inhibited by increases in cytokinin concentrations, regardless of kinds of cytokinin.

  • PDF

A Study on the Wild Exochorda serratifolia for Landscape Horticuitural Cultivation (야생 가침박탈(Exochorda serratifolia)의 조경원예화에 관한 연구)

  • 이기선;한교필;박원조;김일섭
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.15 no.2
    • /
    • pp.139-148
    • /
    • 1987
  • Exochorda serratifolia, broad-leaved shrub has beautiful flowers and is resistant to cold, shade and decease. New this native plant is considered to be worth being exploited as the outstanding plant for landscaping and horticulture. So this study was executed to utilize Exochorda serratifolia as the planting material for landscaping and horticulture through the survey of its habitat environment and the experimennt of its seed physiology and germination, vegetative propagation, culture and utilization, etc.. The results are as follows ; 1. The color of the flower is white, blooming in the early and middle of May and the seeds ripen late in Sep.. 2. The elevation, elevation, gradient and direction of the native habitat were 250m, 20-25$^{\circ}$and northern side respectively. 3. The soil pH of the natural habitat was 5.3 and soil fertility was poor. 4. Exochorda serratifolia appeared as indicator within P. densiflora community and its neighboring species were Q. mongolica, Q. dentata, R. mucronulatum, L. obtusiloba, E. oxyphillus, C. heterolphylla, var thunbergii, etc.. 5. The optimum temperature for seed germination was found at 20$^{\circ}C$ and the longer the stratification period at 5$^{\circ}C$ was, the lower the germination rate was. 6. The treat merits of GA and Kinetin increased the seed germination rate, especially under the dark condition but their high concentrations decreased the seed germination rate. 7. The rooted rate of the greenwood cutting was the highest at IBA 100ppm plot of vermiculite bed but its high concentration decreased the rooted rate conspicuously. 8. In the tissue culture, the each 1.0 ppd plot of NAA, Kinetin and NAA 1.0ppm + Kinetin showed the best growth. And the mixture of NAA 1.0ppm and Kinetin showed better growth than the single treatment of NAA or Kinetin did. 9. Transplanted Exochorda serratifolia showed healthy growth with shaded environmental condition(42.1% light intensity), therefore they can be cultivated as a shade tolerant landscape plant. 10. It was considered that Exochorda serratifolia was applicable to group planting at shade places or under trees in parks, homes, etc..

  • PDF