• Title/Summary/Keyword: kinds of construction

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Construction of a Transgenic Plant to Develop a New Method for the Isolation of Calmodulin-Binding Proteins (새로운 방법을 이용한 칼모둘린 결합 단백질 분리를 위한 형질 전환 식물체의 구축)

  • Kim, Sun-Ho;Lee, Kyung-Hee;Kim, Kyung-Eun;Jung, Mi-Soon;Lim, Chae-Oh;Lee, Shin-Woo;Chung, Woo-Sik
    • Journal of Life Science
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    • v.17 no.9 s.89
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    • pp.1177-1181
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    • 2007
  • Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety CaM-binding proteins (CaMBPs). Because eukaryotes have multiple CaMBPs, it is important to isolate and characterize them in different tissues and conditions. So far a number of CaMBPs have been identified through classical screening methods. Many classes of proteins have been predicted to bind CaMs based on their structural homology with already known targets. In an effort to develop a method for large-scale analysis of CaMBPs in Arabidopsis, we have generated a transgenic plants overexpressing AtCaM2-GFP. We performed protein pull-down assay to test whether exogenously expressed AtCaM2-GFP proteins can interact with CaMBPs. The exogenously expressed AtCaM2-GFP could strongly interact with a CaMBP, AS1 protein. This result suggests that AtCaM2-GFP in transgenic plants may interact with many CaMBPs in plant cell. Therefore, we will be able to isolate kinds of CaMBPs by using these transgenic plants in many different tissue and environments.

Effectiveness of Three Commercial Wood Preservatives against Termite in Korea (주요 국내 사용 방부제 3종에 대한 흰개미 저항 효력)

  • Lee, Hansol;Hwang, Won-Joung;Lee, Hyun-Mi;Son, Dong-Won
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.6
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    • pp.804-809
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    • 2015
  • Since Korea is home to Reticulitermes speratus, a kind of subterranean termites that prefer dark and humid conditions, there have been increasing damages to wooden structures by termites. One noticeable attribute of Korean subterranean termites is that they prefer than Pinus densiflora, the major construction material for Korean traditional houses. And because wide varieties of termites are distributed all over the world, it is not so easy to choose appropriate control methods depending on specific areas. This necessitates careful applications of the following control methods depending on the kinds of termites: fumigation treatment, soil termiticide, preservatives and insect treatment, termite colony elimination system, chemical treatment, and other physical and biological treatment methods. The purpose of this study is to investigate the control effects of environmentally-friendly Alkaline copper quaternary (ACQ), Copper Azole (CuAZ) and Micronized copper quarter (MCQ) on the termites contributing to the damage of wooden structures. It was found in this study that wood with preservative treatment produced a significantly higher termicidal efficacy than untreated wood.

Construction and characterization of the bacteriophage P4 derivatives whose genome size suitable for packaging into a P2sir3-sized head (P2 sir3-sized head에 packaging 되기 용이한 크기의 박테리오파지 P4 유도체 조성과 정성 연구)

  • Kim, Kyoung-Jin
    • Korean Journal of Microbiology
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    • v.51 no.1
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    • pp.1-6
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    • 2015
  • The term "P2 sir-associated helper inefficiency" has been used to define the inefficient helper capability of P2 sir mutants for their satellite bacteriophage P4. The aim of this study was to investigate the factors overcoming P2 sir-associated helper inefficiency. At first, we verified whether the P2 cos region containing P4 sid71 cosP2 could overcome P2 sir-associated helper inefficiency with P2 sir3. The result was that P4 sid71 cosP2 could not overcome P2 sir-associated helper inefficiency with P2 sir3. Instead of cos region of P2, the size of the DNA packaged into a $P2_{sir}$-sized head seems to be important for overcoming P2 sir-associated helper inefficiency. In the present work, three kinds of P4 derivatives with packaged DNA sizes between those of P4 ost1 and P4 ost2, were constructed through DNA manipulation. In one P4 derivative, P4 sid71 delRI::apr, the size of the packaged DNA was identified with a CsCl buoyant equilibrium density gradient experiment. According to the burst sizes of the P4 derivatives, they could overcome P2 sir3-associated helper inefficiency. The size of the P4 derivative DNA suitable for packaging into a $P2_{sir3}$-sized head was 28-29 kb.

Preliminary study on a spoke-type EPB shield TBM by discrete element method (개별요소법을 활용한 스포크 타입 토압식 쉴드TBM의 예비 해석 연구)

  • Lee, Chulho;Chang, Soo-Ho;Choi, Soon-Wook;Park, Byungkwan;Kang, Tae-Ho;Sim, Jung Kil
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.19 no.6
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    • pp.1029-1044
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    • 2017
  • The Discrete Element Method (DEM) is one of the useful numerical methods to analyze the behavior of the ground formation by computing the motion and interaction using particles. The DEM has not been applied in civil engineering but also a wide range of industrial fields, such as chemical engineering, pharmacy, material science, food engineering, etc. In this study, to review a performance of the spoke-type earth pressure balance (EPB) shield TBM (Tunnel Boring Machine), the commercial software based on the DEM technology was used. An analysis of the TBM during excavation was conducted according to two pre-defined excavation conditions with the different rotation speed of a cutterhead. During the analysis, the resistant torque at the face of the cutterhead, the compressive force at the cutterhead and shield surface, the muck discharge at the screw auger were measured and compared. Upon the two kinds of excavation conditions, the applicability of the DEM analysis was reviewed as a modelling method for the TBM.

Behavior of heavy metals in the surface waters of the Lake Shihwa and its tributaries (시화호와 주변 하천 표층수중의 중금속 거동 특성)

  • Kim Kyung Tae;Lee Soo Hyung;Kim Eun Soo;Cho Sung Rok;Park Chung Kil
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.5 no.1
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    • pp.51-67
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    • 2002
  • In order to understand behaviors of heavy metals around the artificial Lake Shihwa in the vicinity of Kyunggi Bay in Korea in relation with huge environmental changes due to construction of huge artificial lake, water samples were collected from Lake Shihwa and its tributaries from 1996 to 1998 and analyzed. Due to extreme pollutant discharge from various kinds of anthropogenic sources such as the Banweol and Shihwa Industrial Complexes and cities, the Shihwa and its tributaries have been polluted in waters with various heavy metals. The enrichment factors of particulate heavy metals in water of streams and storm sewers were very high. All of the heavy metals observed in the waters showed relatively high temporal and spatial variations. In surface waters of the lake during the desalination after the dike establishment, spatial distributions of heavy metal concentrations were mainly controlled by various biogeochemical factors as well as input of industrial and municipal wastewaters, while, physical mixing was minor factor Pb and Co showed a strong affinity to particle phase, however the affinity to dissolved phase was dominated in Ni, Cu and Cd. Water quality of the artificial Lake Shihwa has been deteriorated by direct discharge of untreated wastewater and heavy metals have been accumulated in the lake system. Therefore, luther environmental improvement plan should be programmed subsequently.

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A Study on Analysis and Development of Education Program in Information Security Major (대학의 정보보호 관련학과 교육과정분석과 모델개발에 관한 연구)

  • 양정모;이옥연;이형우;하재철;유승재;이민섭
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.13 no.3
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    • pp.17-26
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    • 2003
  • Recently, as the internet is widespread rapidly among the public, people can use a variety of useful information services through the internet. Accordingly, the protection of information supplied by computer networks 5 has become a matter of primary concern on the whole world. To accede to the realistic demands, it has been worked out some countermeasures to cultivate the experts in information security by the government and many educational facilities. Already the government authority has carried out the each kinds of concerning projects under the framed a policy, Five-Year Development Plan for Information Security Technology. Also, many domestic universities perceives such an international trend, and so they frame their plans to train for the experts in this field, including to found a department with respect to the information security. They are ready to execute their tangible works, such as establishment of educational goal, development of teaching materials, planning curriculum, construction of laboratories and ensuring instructors. Moreover, such universities lead to their students who want to be information security experts to get the fundamental knowledge to lay the foundation for acquiring the information security technology in their bachelor course. In this note, we survey and analyze the curricula of newly-established or member-extended departments with respect to information security fields of some leading universities in the inside and outside of the country, and in conclusion, we propose the effective model of curriculum and educational goal to train the students for the information security experts.

Making a Science Map of Korea (국내 광역 과학 지도 생성 연구)

  • Lee, Jae-Yun
    • Journal of the Korean Society for information Management
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    • v.24 no.3
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    • pp.363-383
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    • 2007
  • Global map of science, which is visualizing large scientific domains, can be used to visually analyze the structural relationships between major areas of science. This paper reviewed previous efforts on global science map, and then tried to making a science map of Korea with some new methods. There are several research groups on making global map of science including Dr. Small and Dr. Garfield of ISI (now Thompson Scientific), SCImago research group at the University of Granada, and Dr. Borner's InfoVis Lab at the Indiana University. They called their maps as science map or scientogram and called the activity of mapping science as scientography. Most of the previous works are based on citations between scientific articles. However citation database for Korean journal articles is still under construction. This research tried to make a Korean science map with the text in the proposals suggested for funding from Korean Research Foundation. Two kinds of method for generating networks of scientific fields are used. One is Pathfinder network (PFNet) alogorithm which has been used in several published bibliometric studies. The other is clustering-based network (CBnet) algorithm which was proposed recently as an alternative to PFNet. In order to take into account both views of the two algorithms, the resulting maps are combined to a final science map of Korea.

A Study on Routing Implementation and Performance Measurement Scheme among IPv6 Terminals (IPv6 단말간 라우팅 구현과 성능측정 구성 방안에 관한 연구)

  • Woo, Jung-Yoon;Yang, JaeSoo;Lee, Jong Won;Kim, Kangju
    • Journal of Digital Contents Society
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    • v.19 no.3
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    • pp.505-512
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    • 2018
  • Internet demand has increased rapidly, and Internet Protocol Version 4, the main Internet protocol, can not cope with various types of network demand in the future. Therefore, it is urgent to spread IPv6-based stable network to solve the shortage of Internet address resources. Due to the proliferation of IoT and the increase in mobile devices, efficient and optimal IPv6 routing design is indispensable. In this paper, we have studied about characteristics of IPv6, address system of IPv6, structure analysis and efficient routing technology among IPv6 terminals. Furthermore, experimental results on routing packet analysis and convergence time are presented in network - based IPv6 routing implementation environment. In addition, we show the results of the experiment analysis by dividing the implementation of IPv6 routing, operation test, and fail over performance into three kinds of routing protocol methods. As a result, this study can be applied to the introduction of IPv6 system, test bed environment construction, and network design.

Discharge Standards of Kitchen-Disposer Wastewater by Treatment Types (디스포저(부엌용 오물분쇄기)-배수 전처리 방식 별 수질기준)

  • Chang, Ho Nam;Jeong, Chang Moon;Kang, Jong Won;Choi, Jin-dal-rae;Park, Young Sook;Ku, Ja-Kong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.19 no.2
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    • pp.55-69
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    • 2011
  • Use of disposer at the kitchens of Korean apartments is inevitable in treating their foodwaste having a water content of more than 80%. Also we have to ensure that this extra disposer-foodwaste BOD loadings be treated properly by installing/operating a pre-treatment system before this wastewater enters public sewer system. However, the degree of BOD removal should not be excessive since a BOD/N ratio higher than 5 is required for removing N/P at a municipal wastewater treatment plant. The removal of BOD/N in the pretreatment system rather than BOD alone can be an alternative solution in solving this problem. The particles separated by sedimentation, screen or packed-bed can be anaerobically digested at apartment sites to generate biogas that can be used for simple digester heating and to generate volatile fatty acids (VFAs) for nitrogen removal. We suggest that Korean government grants a temporary license (say for 5 years) to foodwaste treatment companies in collaboration with apartment construction companies which may do business and develop various kinds of disposer-foodwaste treatment systems in diverse wastewater discharge systems of Korean apartments.

Implementation of Dynamic Situation Authentication System for Accessing Medical Information (의료정보 접근을 위한 동적상황인증시스템의 구현)

  • Ham, Gyu-Sung;Seo, Own-jeong;Jung, Hoill;Joo, Su-Chong
    • Journal of Internet Computing and Services
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    • v.19 no.6
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    • pp.31-40
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    • 2018
  • With the development of IT technology recently, medical information systems are being constructed in an integrated u-health environment through cloud services, IoT technologies, and mobile applications. These kinds of medical information systems should provide the medical staff with authorities to access patients' medical information for emergency status treatments or therapeutic purposes. Therefore, in the medical information systems, the reliable and prompt authentication processes are necessary to access the biometric information and the medical information of the patients in charge of the medical staff. However, medical information systems are accessing with simple and static user authentication mechanism using only medical ID / PWD in the present system environment. For this reason, in this paper, we suggest a dynamic situation authentication mechanism that provides transparency of medical information access including various authentication factors considering patient's emergency status condition and dynamic situation authentication system supporting it. Our dynamic Situation Authentication is a combination of user authentication and mobile device authentication, which includes various authentication factor attributes such as emergency status, role of medical staff, their working hours, and their working positions and so forth. We designed and implemented a dynamic situation authentication system including emergency status decision, dynamic situation authentication, and authentication support DB construction. Finally, in order to verify the serviceability of the suggested dynamic situation authentication system, the medical staffs download the mobile application from the medical information server to the medical staff's own mobile device together with the dynamic situation authentication process and the permission to access medical information to the patient and showed access to medical information.