• 한국수산과학회지
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• 제30권4호
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• pp.589-594
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• 1997

횟감으로 많이 생식되고 있는 넙치육의 사후조기의 물리화학적 변화를 억제시켜서 신선도의 유지기간을 연장시킬 목적으로, 마취사 및 무방혈이 사후조기의 넙치육의 물리화학적 변화에 미치는 영향에 대하여 실험한 결과를 요약하면 다음과 같다. 1. 사후 넙치육의 경직개시 시간 및 완전경직 도달시간은 마취사${\cdot}$무방혈이 즉살${\cdot}$방혈보다 늦었으며, 최고 경직도도 마취사${\cdot}$무방혈이 낮은 값을 나타내었다. 2. ATP 분해도 마취사${\cdot}$무방혈이 즉살${\cdot}$방혈 보다 억제되었다. 즉, 즉살${\cdot}$방혈에서는 저장기간 동안에 꾸준히 감소하여 35시간 후에 완전히 분해되었지만, 마취사${\cdot}$무방혈에서는 저장 22.5시간까지 분해가 억제되다가, 그 후로 급속히 분해되었다. 3. 육의 파괴강도는 즉살${\cdot}$방혈에서 저장 10시간 후에 최대값을 나타내고 그후로 급속히 저하하였으며, 마취사${\cdot}$무방혈에서는 저장 13시간 후에 최고값에 도달한 후에 20시간까지 거의 일정하게 유지되다가 그후로 급속히 저하하였다. 4. 유산의 축적은 즉살${\cdot}$방혈에서는 저장기간을 통하여 꾸준히 증가하여 저장 20시간 후에는 최고값에 도달하였으며, 마취사${\cdot}$무방혈에서는 저장 15시간까지 유산의 생성이 억제되다가, 그후로 급격히 증가하여 저장 25시간 후에 최고값에 도달하였다.

• 약학회지
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• 제55권1호
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• pp.39-44
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• 2011

Many reports indicate that $18{\beta}$-glycyrrhetinic acid ($18{\beta}$-GA) from Glycyrrhizae Radix has anti-inflammatory and immunoregulatory activities, whereas reports regarding anticancer activity of the compound are few. In present study, we investigated antitumor effect of $18{\beta}$-GA on tumor caused by A549 cancer cell in mice. Data resulting from the cytotoxicity assay showed that $18{\beta}$-GA caused killing of A549 cells. $LD_{50}$ values of $18{\beta}$-GA were app. 180 ${\mu}M$ and 80 ${\mu}M$, corresponding to 48 hr- and 72 hr-treatments, displaying that the killing activity was more effective as the $18{\beta}$-GA treatment was prolonged. Based on these data, antitumor effect of $18{\beta}$-GA was tested in nude mice. For induction of the tumor, A549 ($3{\times}10^6$ cells/mouse) was injected subcutaneously into the lateral abdomen of nude mice (Balb/c nu/nu). To determine the antitumor effect, nude mice with tumor were given $18{\beta}$-GA (1 mg/200 ${\mu}l$/mouse) intraperitoneally every three days for four times. Tumor-sizes were measured with a caliper for a period of 24 days. Results showed that the $18{\beta}$-GA treatment reduced the tumor-sizes (P<0.05) as compared with negative control nude mice that received diluent (DPBS). The reduction degree was greater than reduction degree by doxorubicin (60 ${\mu}g$/mouse), and the pattern of reduction was almost sustained during the entire period of the observation. In conclusion, our studies demonstrate that $18{\beta}$-GA has antitumor activity to the A549 cancer cell-caused tumor.

• 한국식품위생안전성학회지
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• 제13권4호
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• pp.365-369
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• 1998

가금류 가공등에서 사용이 허가된 Trisodium Phoshate(TSP)와 구강 청결제로 사용되면서 안전한 물질로 알려진 Cetylpyridinium chloride(CPC)를 이용하여 in vitro에서 E. coli O157:H7 및 Listerial monocytogenes에 대하여 살균작용을 조사하였다. E. coli O157:H7 및 Listerial monocytogenes에 대해서는 TSP는 $1{\times}10^{-2}\;M$ 농도에서} $37^{\circ}C$, 30분간 반응으로 각각 30~40%와 40~50%의 살균효과를 보였으며, CPC는 $5{\times}10^{-7}\;M$ 농도에서 $37^{\circ}C$, 30분간 반응으로 각각 90~95%와 95~99%의 살균효과를 보였다. 또한, TSP와 CPC는 온도와 pH에 따라 E.coli O157:H7 및 Listerial monocytogenes에 대한 살균작용이 영향을 받았는데, 온도가 높을수록 두 물질 모두 그 살균 효과 컸으며, TSP는 pH가 높을수록, CPC는 pH가 낮을수록 살균효과가 컸다. 이러한 결과로 보아 TSP와 CPC도 E. coli O157:H7 및 Listerial monocytogenes의 오염방지를 위하여 사용이 가능할 것으로 본다.

• Biomolecules & Therapeutics
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• 제30권5호
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• pp.418-426
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• 2022

Chimeric antigen receptor T (CAR-T) cell therapy is one of the promising anticancer treatments. It shows a high overall response rate with complete response to blood cancer. However, there is a limitation to solid tumor treatment. Additionally, this currently approved therapy exhibits side effects such as cytokine release syndrome and neurotoxicity. Alternatively, bispecific antibody is an innovative therapeutic tool that simultaneously engages specific immune cells to disease-related target cells. Since programmed death ligand 1 (PD-L1) is an immune checkpoint molecule highly expressed in some cancer cells, in the current study, we generated αCD3xαPD-L1 bispecific antibody (BiTE) which can engage T cells to PD-L1⁺ cancer cells. We observed that the BiTE-bound OT-1 T cells effectively killed cancer cells in vitro and in vivo. They substantially increased the recruitment of effector memory CD8⁺ T cells having CD8⁺CD44⁺CD62L^low phenotype in tumor. Interestingly, we also observed that BiTE-bound polyclonal T cells showed highly efficacious tumor killing activity in vivo in comparison with the direct intravenous treatment of bispecific antibody, suggesting that PD-L1-directed migration and engagement of activated T cells might increase cancer cell killing. Additionally, BiTE-bound CAR-T cells which targets human Her-2/neu exhibited enhanced killing effect on Her-2-expressing cancer cells in vivo, suggesting that this could be a novel therapeutic regimen. Collectively, our results suggested that engaging activated T cells with cancer cells using αCD3xαPD-L1 BiTE could be an innovative next generation anticancer therapy which exerts simultaneous inhibitory functions on PD-L1 as well as increasing the infiltration of activated T cells having effector memory phenotype in tumor site.

• 대한한방내과학회지
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• 제12권2호
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• pp.1-15
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• 1991

Bigihwan which has been widely used in Oh-jug in oriental Medicine was investigated on its antitumor effect employing blood cancer cell lines. K 562 derived from human erytholeukemia, Raji from lymphoma and $MO_4$ from hlastogenic cancer were used in this study to see the analytical evaluation of Bigihwan' s antitumor effect using three different kinds of methods such as $^{3}H-thymidine$ up take assay. MTT assay and live cell counts by Trypan blue assay. The result obtained are as follows. 1. When higher than 10% Bigihwan was treated. inhibitory effect of tumor killing action was observed showing the increasing order of $MO_4$, K 562 and Raji(Fig. 3). 2. When 1 to 5% of Bigi-hwan was treated, 4 to 30% of tumor cell survival was observed according to various blood tumor cell lines suggesting that antitumor effect of Bigi-hwan was different as the characteristics of tumor cells showing 70 to 95% cell killing effent(Fig. 4). 3. Compared the survivals of cells by relative scales though the initial cpm was variable because of different cell growth rate. Raji was most effective being killed 95% by the treatment of 1% Bigihwan while Raji and K562 showed 93% by 5% Bigihwan.(Fig. 5) 4. The survival rate of Raji derived from Burkitt lymphoma was rather increased to 2.3 times when Bigihwan concentration was increased from 1 to 10% lmplying of refraining from over use of this anticancer drug. specially to lymphoma patients(Fig. 5). 5. Bigihwan was most effective to K 562 and then $MO_4$ showing 95% tumor cell death by using 1% of this anticancer drug while it was least effective to Raji showing only 68% of tumor cell death(Fig.7). 6. Judging from the all the analytical methods used in this study, through all different three tumor cell lines. Bigihwan was most effective to K 562 derived from human erythroleukemia.

• 한국수산과학회지
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• 제31권5호
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• pp.767-773
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• 1998

1996년 6월부터 10월까지 적조다발해역 마산만에서 C. polykrikoides 살조세균의 분포와 분리된 110 균주 중 살조능이 우수한 Micrococcus sp. LG-1의 살조특성을 요약하면 다음과 같다. 현장해수에서 C. polykrikoides 살조세균은 $10^2\~10^3$cells/ml의 범위로, C. polykrikoides 적조가 발생한 9월에 $4.8\times10^3$cells/ml로 가장 높았으며, 적조가 소멸하는 10월에 $2.0\times10^2$cells/ml로 감소하는 경향을 나타내었다. C. polykrikoides에 대한 Micrococcus sp. LG-1의 살조특성을 혼합배양을 통하여 조사하였다. 잠복기와 대수증식기의 C. polykrikoides의 배양액에 Micrococcus sp. LG-1을 접종한 결과 각각 6일과 5일 만에 사멸되어, 대수증식기의 C. polykrikoides가 빠르게 사멸되었다. 또한, Micro-coccus sp. LG-1의 개체수와 배양여과액의 농도에 비례하여 C. polyklikoides의 사멸효과가 높게 나타났으며, 배양여과액은 최종농도가 2.5\%$일 경우는 접종 후 5.5시, $5\%$에서는 3.5시간, 10과 $20\%$에서는 1.5시간 그리고 $30\%$에서는 1시간만에 모두 사멸하였다. Alexandrium tamarense, Prorocentrum micans, Scrippsiella trochoidea. ana Gymnodinium sanguineum, Cochlodinium polykrikoides의 5종의 적조원인 편모조류에 대한 Micrococcus sp. LG-1의 살조특이성은 C. polykrikoides에만 살조효과를 나타내었다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제13권4호
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• pp.391-404
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• 1991

Three anticncer agents which are different in time or dosage dependence as well as in phase specificity, namely mitomycin and adriamycin from natural products, and widely different cancer cell lines_Four epidermoid carcinomas originated from larynx, cervix, skin and gut were used toghether with one osteosarcoma as the target cell of single and combined administration of anticancer drugs. Semiautomated tetrazolium dye assay(MTT) appears to offer an attractive option for chemosensitivity of head and neck cancers since it is a simple, valid and inexpensive method of assessing chemosensitivity for large samples in a short time. The results obtained form this study were as follows. 1. Good correlations were obtained with the results of the MTT test and those of $^3H$ thymidine uptake assay. 2. $LD_{50}$ values of HIST and St.Ca. which showed relatively high doubling time on adriamycin were $30{\mu}g/ml$ and $15{\mu}g/ml$ while those of HeLa, Hep-2 and KHOS/NP were $2.1{\mu}g/ml$, $4.8{\mu}g/ml$, and $6.8{\mu}g/ml$ respectively. 3. The $LD_{50}$ value of 5-FU on five cancer cells were very high ranging from 15mg/ml to almost indefinite number, which means 5-FU is very resistant to epidermoid carcinomas or osteosarcoma examined in this study. 4. Mitomycin was relatively effective showing 80% cancer killing effect on HeLa, 70% on St. Ca. and 50% on Hep-2 at the high concentrations used. 5. Adriamycin was the most effective showing 90% cancer cell killing effect on KHOS/NP, 98% on HeLa, 80% both on Hep-2 and St. Ca. The least susceptible cancer cells toward adriamycin was HIST having only 55% cell killing effect at the high cincentration. 6. Combined therapy of adriamycin and 5-FU was more effective than single administration in all the cases examined. Most effective synergism was observed on St. Ca. at the low concentration, showing 21 times higher than each single administration.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1403-1410
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• 2014

Epigenetic regulators like histone deacetylases (1 and 2), and viral onco-proteins (E6/E7) are known to be overexpressed in cervical cancer cells. The present study was designed to investigate the effect of curcumin on HDACs (1 and 2) and HPV E6/E7 in the cervical cancer cell line SiHa and a drug resistant clone $SiHa^R$ (derived from SiHa). It was further intended to investigate whether curcumin could sensitize the cells towards cisplatin induced cell killing by modulation of multi drug resistant proteins like MRP1 and Pgp1. Curcumin inhibited HDACs, HPV expression and differentially increased acetylation and up-regulation of p53 in SiHa and $SiHa^R$, leading to cell cycle arrest at G1-S phase. Up-regulation of pRb, p21, p27 and corresponding inhibition of cyclin D1 and CDK4 were observed. Cisplatin resistance in $SiHa^R$ due to over-expression of MRP1 and Pgp1 was overcome by curcumin. Curcumin also sensitized both the cervical cancer cells towards cisplatin induced cell killing. Inhibition of HDACs and HPVs led to cell cycle arrest at G1/S phase by alteration of cell cycle regulatory proteins. Suppression of MRP1 and Pgp1 by curcumin resulted in sensitization of cervical cancer cells, lowering the chemotherapeutic dose of the drug cisplatin.

• 대한약침학회지
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• 제19권1호
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• pp.45-50
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• 2016

Objectives: The purpose of this study was to investigate the antifungal effect of bee venom (BV) and sweet bee venom (SBV) against Candida albicans (C. albicans) clinical isolates. Methods: In this study, BV and SBV were examined for antifungal activities against the Korean Collection for Type Cultures (KCTC) strain and 10 clinical isolates of C. albicans. The disk diffusion method was used to measure the antifungal activity and minimum inhibitory concentration (MIC) assays were performed by using a broth microdilution method. Also, a killing curve assay was conducted to investigate the kinetics of the anti-fungal action. Results: BV and SBV showed antifungal activity against 10 clinical isolates of C. albicans that were cultured from blood and the vagina by using disk diffusion method. The MIC values obtained for clinical isolates by using the broth microdilution method varied from $62.5{\mu}g/mL$ to $125{\mu}g/mL$ for BV and from $15.63{\mu}g/mL$ to $62.5{\mu}g/mL$ for SBV. In the killing-curve assay, SBV behaved as amphotericin B, which was used as positive control, did. The antifungal efficacy of SBV was much higher than that of BV. Conclusion: BV and SBV showed antifungal activity against C. albicans clinical strains that were isolated from blood and the vagina. Especially, SBV might be a candidate for a new antifungal agent against C. albicans clinical isolates.

• 대한수의학회지
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• 제2권1호
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• pp.15-26
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• 1962

Decoctions of two Korean indigenous crude drugs. Radix Jingyu(the dried root of Aconitum pseudo-laeve Nakai var. erectum Nakai form. Genuinum Nakai) and Fructus ponciri (the transversely sliced and dried unripe fruit of Poncirus trifoliata Rafinesque), are being utilized by the inhabitants in certain parts of southeastern district of Korea with an empirical belief that these serve as effective insecticides for cattle ticks. Of the two species of cattle ticks so far identified in Korea, Boophilus microplus and Haemaphysalis bispinosum, the former was employed in this experiment as the species occupies nearly 99 percent of the tick population. The results of the experiments herein reported seen to indicate that although the killing power of aqueous extracts of the drugs is minimal, the repellent effect against the ticks, especially of Radix Jingyu, is excellent. It may be of interest to note that the aqueous extract of Radix Jingyu has not proved to be inferior to any of the most effective cattle repellents commercially available. Further studies on these Korean indigenous crude drugs as cattle tick repellents are indicated to find whether active ingredients can be extracted in a hope to materialize an extensive application of these drugs in the field.