• Asian Journal for Public Opinion Research
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• v.5 no.4
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• pp.319-345
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• 2018

Effective higher educational management in undergraduate nursing programs is an important issue from the viewpoint of stakeholders. This qualitative research aimed to examine the characteristics of nursing students and curriculum development of undergraduate nursing education from the opinions of Boromarajonani College of Nursing Saraburi, Thailand stakeholders. The population included 4 groups: 1) the alumni who have graduated within the past 5 years and currently work in primary, secondary, and tertiary care units, 2) the supervisors and colleagues of the alumni, 3) nursing lecturers, and 4) the current nursing students. The respondents who are the alumni, nursing lecturers, and current nursing student were selected using a purposive sampling, for the supervisors and colleagues were selected using snowball techniques. Semi-structured interview questions were used for data collection. Group discussions were conducted until saturation on 55 key informants. The qualitative data was analyzed using content analysis. Results showed the viewpoints of stakeholders on the characteristics of future nurse graduates were comprised of four elements: knowledge that meets standards; essential skills for self-development and lifelong learning process; good morals and professional ethics in providing nursing care; and nurse competencies in teamwork, communication, language, research, management, IT, life skills, and global literacy. The viewpoints on the development of the nursing curriculum focus on four elements: the learner, teaching and learning, course content, and instructor tasks. For learners, the admission criteria should include a minimum not only of knowledge, but also positive attitude, science, and art skills, since the nursing profession is both a science and the art of caring. Teaching and learning elements should be authentic, including exposure to real situations, an integrated network, and activities that improve nursing care. Course content was comprised of an updated curriculum, humanized nursing care, student center, theory and practice with moral integration, case-based study, critical thinking, multidisciplinary work, and love for the nursing profession. Instructor tasks are to elicit student ideas, provide opportunities to learn, support infrastructure, support technology use, and extra-curricular activities to develop the competencies of nursing students. Recommendations were that the curriculum administration should review the selection process of student candidates and instructional management to achieve expected outcomes of nursing characteristics in the future. The nurse lecturer should provide authentic and integrated instruction, decrease lecturing, cultivate a lifelong learning process, and sustain the nursing characteristics.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.923-931
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• 2015

This study was conducted to investigate petal pigmentation and the expression patterns of anthocyanin-related genes in bicolored roses 'Pinky Girl' treated with temporal heat stress (THS). Cyanin accumulation in petals was correlated with floral bud development and started rapidly as floral buds began to open, defined as the $4^{th}$ stage of floral bud development ($S_4$). This stage seems to be most susceptible to petal pigmentation. The total of cyanin pigmentation at blooming was significantly decreased (by 45.5%) with exposure to THS ($39/18^{\circ}C$ for three days at $S_4$) in comparison with control. Meanwhile, the expression of anthocyanin-related genes such as CHS, CHI, F3'H, DFR, ANS, 3GT, and 5GT was relatively promoted by THS. Only F3H was less expressed (by 26.7%) with THS treatment; thus, F3H could be a key gene for bicolor promotion in 'Pinky Girl' among anthocyanin-related genes. Overall, the expression pattern of the most anthocyanin-related genes did not match the trends of cyanin pigmentation in petals. These results suggest that floral pigmentation could be associated with other mechanisms related to anthocyanin biosynthesis such as post-translational effects and regulatory genes.

• Toxicological Research
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• v.11 no.1
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• pp.1-8
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• 1995

Fumonisins are a family of mycotoxins produced by the fungus Fusarium moniliforme which is a common contaminant in corn. Fumonisins are potent inhibitors of sphingosine and sphinganine N-acyltransferase (ceramide synthase), key enzymes in sphingolipid metabolism. The purpose of this study was to provide the evidence that the elevated levels of free sphingoid bases (primarily sphinganine) and depletion of complex sphingolipids were closely related to the inhibition of cell growth in LLC-$PK_1$ cells exposed to fumonisin $B_1$$(\leq 35 {\mu}M)$. Concentrations of fumonisin $B_1$ between 10 and $35 {\mu}M$ were known to inhibit cell growth without cytotoxicity in $LLC-PK_1$ cells (Yoo et al. Toxicol. Appl. Pharmacol. 114, 9-15, 1992). Cells exposed to 35$\mu M$ fumonisin B$_1$ for 48 and 72 hr developed a fibroblast-like (elongated and spindle-shaped) appearance and were less confluent than normal cells. At between 24 and 48 hr after exposure to fumonisin $B_1$ cells were beginning to show the inhibition of cell growth and at 72 hr the number of viable cells in fumonisin-treated cultures was about 50% of concurrent control cultures. During the 24 hr lag period preceding inhibition of cell growth, the free sphinganine levels in cells exposed to $35 {\mu}M$ fumonisin $B_1$ were highly elevated (approximately 230 fold higher than normal cells). The elevated levels of free sphinganine were $435\pm14$$pmoles/{10^6}$ cells at 48 hr and approximately TEX>$333\pm11$$pmoles/{10^6}$ cells in cells exposed to $35{\mu}M$ fumonisin$B_1$ at 72 hr, while the levels of free sphinganine in normal cells were less than 2$pmoles/{10^6}$ cells. Under the same condition, depletion of intracellular complex sphingolipids as a consequence of fumonisin inhibition of de novo sphingolipid biosynthesis and turnover pathway was appeared. Content of free sphingold bases in dividing cells was more elevated than in confluent cells at 24-48 hr after cells were exposed to $20{\mu}M$ fumonisin $B_1$. The dividing cells were showing the inhibition of cell growth at 48-72 hr and $20{\mu}M$ fumonisin $B_1$. The results of this study support the hypothesis that the inhibition of cell growth is very well related to the disruption of sphingolipid metabolism in $LLC-PK_1$ cells.

• Journal of Oriental Neuropsychiatry
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• v.19 no.3
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• pp.179-193
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• 2008

Objective: The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H202-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods: The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions: 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions. 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

• Journal of the Korean Graphic Arts Communication Society
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• v.31 no.1
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• pp.39-49
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• 2013

Nowadays the printing market has shift from 2D(two dimension) industry to 3D(three dimension) industry, along with IT. With rapidly changing market, it's true that the effort to develop the printing materials and upgrade the printing qualities is produced actively in the country. Particularly, the differences in printing materials can often determine the quality of printings in the printability. In the case of the printability of offset, 9 items(paper, ink, order of printing, ink supply, blowing-up halftone, grey balance, key color(base color), overlap efficiency, quality at the halftone dot reproduction etc.) are usually considered as the important factors and used to estimate the printability at abroad. However, in domestic, ink-jet paper is not used for the printing/publishing by ink-jet printing for digital, but done mainly for offset printing. Because the paper of gloss coated type, which is the ink-jet paper, has high price, it's too hard to manufacture massively printing/publishing. Also, in the case of the paper of uncoated type, in spite of its reasonable price rather than the former, the type of uncoated one also has demerit that the ink-jet paper has a clear difference between the front and the back. Therefore, it's tough reality that Ink Jet paper is hardly taken advantage of as the printing/publishing. Thus, what is done in the first to print and/or publish on ink-jet paper is that the front and the back must be printed evenly, and appropriate to the printability of ink-jet. Although there're some kinds of ink-jet paper in the country, it has not been produced in true domestic production yet. In addition, thanks to the brightness of fluorescence brightening agent, the foreign product can offer the good-qualified one-time printing. But when it comes to the printing/publishing, there're difficulties that it may cause the Ocular Fatigue because of long-term exposure of the eyes to text. This research tries to find out the optimum ink-jet paper in domestic for the printing/publishing by studying on the printability of ink-jet on digital web-printing. And it also tries to look for the paper's brightness which can be reduce the possibility to cause the consumers' Ocular Fatigue.

• Journal of the Korean earth science society
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• v.24 no.8
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• pp.657-667
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• 2003

Deep-drilled core sampling and high-resolution seismic survey were carried out to identify a Holocene-late Pleistocene boundary in Gyeonggi Bay, western coast of Korea. Analysis of core sections revealed the existence of an oxidized and semi-consolidated sediment layer, Iying immediately below a Holocene horizon (Unit I) and being developed at the top of a late Pleistocene deposit (Unit II). The oxidized sedimentary layer (uppermost part of Unit II) is characterized by semi-consolidated, yellowish sediments showing signs of desiccation and alteration such as high N value, low water content, periglacial cryogenic structure, depletion of smectite, and high geochemical weathering index (Ba/Sr ratio). This feature, together with radiocarbon ages, suggests that the layer has formed as a result of prolonged subaerial exposure of Unit II sediments during the late Wisconsin sea-level lowstand, producing a regional unconformity. Such unconformitic-bounding surface corresponds to a prominent near-surface reflector (R), which is observed in seismic profiles obtained across the drilled-core sections in the study area. Consequently, the buried oxidized-sedimentary layer associated with the seismic reflector possibly plays a key horizon for the understanding of late Quaternary environmental changes as well as evidence of the emergence of the Yellow Sea shelf during the late Wisconsin sea-level lowstand.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1519-1529
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• 2016

Background: Matrix metalloproteinase -2 (gelatinase-A, Mr 72,000 type IV collagenase, MMP-2) and -9 (gelatinase-B, Mr 92,000 type IV collagenase, MMP-9) are key molecules that play roles in tumor growth, invasion, tissue remodeling, metastasis and stem-cell regulation by digesting extracellular matrix barriers. MMP-2 and -9 are well known to impact on solid cancer susceptibility, whereas, in hematological malignancies, a paucity of data is available to resolve the function of these regulatory molecules in bone marrow mononuclear cells (BM-MNCs) and stromal cells of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Objectives: The present study aimed to investigate mRNA expression and gelatinase A and B secretion from BM-MNCs in vitro and genotypic associations of MMP-2 (-1306 C/T; rs243865), MMP-9 (-1562 C/T; rs3918242), tissue inhibitor of metalloproteinase -1 (TIMP-1) (372T/C; rs4898, Exon 5) and TIMP-2 (-418G/C; rs8179090) in MDS and AML. Results: The study covered cases of confirmed MDS (n=50), AML (n=32) and healthy controls (n=110). MMP-9 mRNA expression revealed 2 fold increased expression in MDS-RAEB II and 2.5 fold in AML M-4 (60-70% blasts). Secretion of gelatinase-B also revealed the MMP-9 mRNA expression and ELISA data also supported these data. We noted that those patients having more blast crises presented with more secretion of MMP-9 and its mRNA expression. In contrast MMP-9 (-1562 C/T) showed significant polymorphic associations in MDS (p<0.02) and AML (p<0.02). MMP-9 mRNA expression of C/T and T/T genotypes were 1.5 and 2.5 fold increased in MDS and AML respectively. In AML, MMP-2 C/T and T/T genotypes showed 2.0 fold mRNA expression. Only MMP-9 (-1306 C/T) showed significant 4 fold (p<0.001) increased risk with chemical and x-ray exposed MDS, while tobacco and cigarette smokers have 3 fold (p<0.04) risk in AML. Conclusions: In view of our results, MMP-9 revealed synergistic secretion and expression in blast crises of MDS and AML with 'gene' polymorphic effects and is significantly associated with increased risk with tobacco, cigarette and environmental exposure. Release and secretion of these enzymes may influence hematopoietic cell behavior and may be important in the clinical point of view. It may offer valuable tools for diagnosis and prognosis, as well as possible targets for the treatments.

• Toxicological Research
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• v.22 no.1
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• pp.23-30
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• 2006

Among poly aromatic hydrocarbons, dioxin and PCBs are the most controversial environmental pollutants in our modern life. These pollutants are known as human carcinogens, and liver is the most sensitive target in animal cancer models. Specific aims of the study were focused on the mechanism of carcinogenesis in hepatocytes and the structure-activity relation among these diverse environmental chemicals. Because key mechanisms of dioxin-induced carcinogenesis in human epithelial cell model are the alteration of signal transduction pathway and PKC isoforms, the alteration of the signal transduction pathways and other factors associated with carcinogenesis were studied. Rat hepatocytes cultured under the sandwich protocols were exposed with the various concentration of dioxins and PCBs, and signal transduction pathway, protein kinase C isoforms, oxidant stress, and apoptotic nuclei were evaluated. Since it is important to understand the structure-activity relation among these chemicals to properly assess the carcinogenic potentials, the study analyzed the parameters associated with carcinogenic processes, based on their structural characteristics. In addition, signal transduction pathways and PKC isoforms involved in inhibition of UV-induced apoptosis were also analyzed to elaborate the tumor promotion mechanism of these chemicals. Induction of apoptosis by UV irradiation was optimal at $60\;J/m^2$ in primary hepatocyte in culture. Compared to non coplanar PCBs such as PCB 114 and PCB 153, coplanar PCBs such as PCB 77 and PCB126 showed a stronger inhibition of apoptosis induced by UV irradiation. Production of reactive oxygen species (ROS) was more stimulated by non-coplanar PCBs than coplanar PCBs with the most potent induction of ROS by chlorinated non-coplanar PCB. As compared to the level of induction by PCB126, non-coplanar PCB153 showed a higher increase of intracellular concentrations. Besides the alteration of intracellular calcium concentration, translocation of PKC from cytosolic fraction to membrane fraction was clearly observed upon the exposure of non-coplanar PCB. Taken together, the present study demonstrated that there is a potent structure-activity relationship among PCB congeners and the mechanism of PAH-induced carcinogenesis is structure-specific. The study suggested that more diverse pathways of PAH-induced carcinogenesis should be taken into account beyond the boundary of Ah receptor dogma to assess the health impact of PAH with more accuracy.

• Journal of Korean Society of Steel Construction
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• v.27 no.2
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• pp.143-153
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• 2015

In this paper, the standard fire resistance test under load and associated numerical study were carried out to evaluate the fire resistance of unprotected partially encased beams and slimfloor beams. The temperature evolution and the deflection increase of the composite beam specimens were investigated and the effects of the key behavioral parameters including the load ratio, the reinforcement, and the fire exposure were analyzed. The test results showed that the temperature rise of the partially encased beams and slimfloor beams is considerably slow compared to the conventional H-shape composite beams. Up to at least 90 minutes, the reinforcements in the partially encased composite beams maintained below the temperature at which the cold steel strength is sustained. Unprotected partially encased beams and slimfloor beams in the experimental program achieved the fire resistance more than 2 hours according to the limiting deflection criteria. This implies that unprotected partially encased beams and slimfloor beams can be very promising alternatives to enhancing the fire resistance of steel beams. This study also conducted the fully coupled thermal-stress analysis by using the commercial code ABAQUS to the thermal and structural behaviour of composite beams in fire. The numerical predictions provide acceptable correlations with the experimental results.

• Journal of Science and Technology Studies
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• v.18 no.1
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• pp.129-175
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• 2018

This paper examines asbestos activism in South Korea by focusing on the politics of knowledge between the asbestos activist group and regulatory agency on the risk of asbestos exposure. Asbestos activism has contributed to establishing asbestos pollution an important safety and public health agenda in South Korea. Asbestos pollution investigation is key to core argument of the activism that asbestos pollution is pervasive especially in urban environment and a serious environmental health problem with its worst consequences has not yet seen. A distinctive characteristic of such asbestos investigation is the use of "settled dust analysis," non-standard, non-legislated analysis method. In this paper, literary technologies used in asbestos investigation report written by activists and controversies over asbestos pollution measurement in Samsung's head office building. Asbestos activists successfully concentrated media's attention on their argument and mobilize resources needed to make policy decisions, by using settled dust analysis data. Regulatory agency and expert group, however, neither saw settled dust analysis nor activists argument persuasive enough to make policy changes, base on their evaluation on the use of standards and evidentiary context for analyzing measured data. While its explanatory power is partially acquired, through the dispute between asbestos activists and regulatory agencies unspoken assumptions of regulatory science was revealed and became the matter of social debate. Settled dust analysis captures the characteristic of asbestos analysis which combined social movement and science to challenge the regulatory agency and expert group.