• Journal of Microbiology and Biotechnology
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• 제2권4호
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• pp.237-242
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• 1992

In order to clone the gene coding for alkaline phosphatase in the yeast Kluyveromyces fragilis, a genomic library was constructed using the yeast-E. coli shuttle vector pHN114 as a cloning vector. From the genomic library, a clone carrying the gene was isolated and the plasmid was designated as pSKH101. A restriction enzyme map was made using this plasmid. Subcloning experiments and complementation studies showed that alkaline phosphatase was active only in the original 3.1 kb insert. Southern hybridization analysis confirmed that the cloned DNA fragment was derived from K. fragilis genomic DNA. Using a minicell experiment, the product of the cloned gene was identified as a protein with a molecular weight of 63 KDa. A 0.6 kb HindIII fragment, which showed promoter activity, was isolated using the E. coli promoter-probe vector pKO-1.

• 정보관리연구
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• 제30권4호
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• pp.27-38
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• 1999

도서관이 정보센터로 변화해 가는 사회환경에서 정보의 축적 및 이용형태도 디지털화되고 있다. 이런 관점에서, 디지털 정보의 이용과 저작권의 개념에 관하여 검토하고, 정보전문가로서의 역할을 재조명한다.

• 한국문헌정보학회지
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• 제8권
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• pp.103-128
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• 1981

This study has two aims. one is to devide the ideology of reference service into three groups such as conservative, moderative, and progressive theories, and then investigate the validity of each theory as well as any problems to be suggested. The other is to establish appropriate standards of reference service according to the types of libraries and users, based on the analysis of such theories. Each theory has its own merits and demerits. Accordingly, it seems to be difficult to apply uniformly and theory into users who have different types of question and educational levels. And also it is difficult to apply into various libraries which have different aims of establishment, characteristics, and the users of each library. Consequently, it is suggested to set up a norm of reference service according to the types of libraries and users respectively. The conservative theory is applicable to small and medium sized public libraries, school libraries, and college and university libraries of which the users are mainly students and citizens. The progressive theory is desirable for special and academic libraries as well as large public libraries where the users are mainly specialists. and scholars.

• 한국문헌정보학회지
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• 제20권
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• pp.127-161
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• 1991

This study concerns with the reasons of the revision of the classification scheme and the kinds and methods of the reclassification. The reclassification IS to be implemented in case that classification numbers are wrongly given, or the scheme is revised, or it is wanted that presently using scheme should be changed to a different one. In the case of a revised edition, it is desired that reclassification is made based on the new edition because of the modernization of a data organization. However, in case of that it is not possible for the situations in the library such as the number of collections, staffs, facilities, budget, etc., the old edition can be based and the new one can be referred to. In this case, however, classification numbers may be dualized on one subject, and therefore, library must prepare the reference cards and the marks of the shelves for the different class numbers. Also, because much budget is required when the scheme is changed to another one due to its unsatisfactory usage, it should be carefully considered whether to change or not. The required time in reclassification for the relocated classification number of the revised edition is 18 minutes 54 seconds per volume, and its cost requires W 1,224.

• 한국도서관정보학회지
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• 제32권4호
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• pp.361-394
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• 2001

이 연구는 대학도서관(전문대학 포함)의 정리업무의 아웃소싱 적용현황을 조사, 분석하여 앞으로 도서관에서 아웃소싱을 적용할 때 고려해야 할 사항과 정책수립에 필요한 방향을 제시하고자 시도되었다. 연구방법은 문헌연구와 설문지 기법을 병행하였다. 분석 결과 아웃소싱을 시행한 도서관은 대개 그 결과에 만족했으며, 대상 업무는 아직까지 목록에 집중되고 있었다. 아웃소싱에 도서관 직원이 참여하는 것이 효율이 높았으며, 아웃소싱 업체의 전문성 부족을 문제점으로 지적했다. 앞으로도 아웃소싱을 고려할 수 없는 업무로는 참고업무를 첫째로 꼽았다.

• 한국문헌정보학회지
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• 제9권
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• pp.99-118
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• 1982

This paper first reviews various techniques proposed for automatic indexing with special emphasis placed on statistical techniques. Frequency-based statistical techniques are categorized into the following three approaches for further investigation on the basis of index term selection criteria: term frequency approach, document frequency approach, and probabilistic approach. In the experimental part of this study, Pao's technique based on the Goffman's transition region formula and Harter's 2-Poisson distribution model with a measure of the potential effectiveness of index term were tested. Experimental document collection consists of 30 agriculture-related documents written in Korean. Pao's technique did not yield good result presumably due to the difference in word usage between Korean and English. However, Harter's model holds some promise for Korean document indexing because the evaluation result from this experiment was similar to that of the Harter's.

• 한국문헌정보학회지
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• 제45권1호
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• pp.287-310
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• 2011

이 연구는 우리나라 대학생 집단을 대상으로 대학도서관 전자책 서비스 만족도를 조사하였다. 전자책 이용에 대한 현황을 파악하고자 이용행태를 조사하였고, 전자책 이용행태와 전자책 서비스품질차원에 따른 만족도를 분석하였다. 분석결과, 대학도서관 전자책 서비스에 대한 전반적인 만족도는 평균 2.93으로 보통인 것으로 조사되었다. 또한 이용빈도가 높은 이용자일수록 만족도가 높았고, 저학년 집단에 비해 고학년 집단과 대학원집단이 전자책서비스에 대한 전반적인 만족도에서 유의미하게 높은 것으로 나타났다. 한편, 만족도에 가장 큰 영향을 미치는 전자책서비스품질차원은 콘텐츠이며, 도서관 지원서비스, 시스템 순으로 영향을 미쳤다. 이상의 분석결과를 토대로 향후 전자책에 기반한 대학도서관 서비스를 활성화하기 위해서는 도서관 홍보를 비롯하여 이용자 교육의 강화, 콘텐츠의 다양성과 최신성을 유지할 것을 제안하였다.

• Journal of Microbiology and Biotechnology
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• 제3권1호
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• pp.6-11
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• 1993

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

• 정보관리연구
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• 제26권4호
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• pp.28-62
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• 1995

본 연구는 이용자 지향적인 서비스 개선방법을 모색하기 위해 끊임없이 변화하고 있는 인터넷에 대하여 전반적으로 기술하였다. 특히 정보검색 측면에서 현재 널리 이용되고 있는 Gopher, Veronica, Archie, WAIS, WWW 등의 전반적인 개념과 특성 그리고 사용방법을 설명하였다. 그리고 WWW의 웹브라우저와 검색엔진에 관해서는 실제 사용한 경험을 근거로 상세하게 설명함으로써 초보자도 필요한 장비만 갖추어진다면 인터넷을 사용할 수 있는 방법을 소개하였다.

• Journal of Information Processing Systems
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• 제16권3호
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• pp.599-611
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• 2020

With the application and promotion of biometric technology, biometrics has become more and more important to identity authentication. In order to ensure the privacy of the user, the biometrics cannot be stored or manipulated in plaintext. Aiming at this problem, this paper analyzes and summarizes the scheme and performance of the existing biometric authentication system, and proposes an iris-based ciphertext authentication system based on fully homomorphic encryption using the FV scheme. The implementation of the system is partly powered by Microsoft's SEAL (Simple Encrypted Arithmetic Library). The entire system can complete iris authentication without decrypting the iris feature template, and the database stores the homomorphic ciphertext of the iris feature template. Thus, there is no need to worry about the leakage of the iris feature template. At the same time, the system does not require a trusted center for authentication, and the authentication is completed on the server side directly using the one-time MAC authentication method. Tests have shown that when the system adopts an iris algorithm with a low depth of calculation circuit such as the Hamming distance comparison algorithm, it has good performance, which basically meets the requirements of real application scenarios.