• BMB Reports
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• 제44권5호
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• pp.291-297
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• 2011

Aging is one of the most complicated biological processes in all species. A number of different model organisms from yeast to monkeys have been studied to understand the aging process. Until recently, many different age-related genes and age-regulating cellular pathways, such as insulin/IGF-1-like signal, mitochondrial dysfunction, Sir2 pathway, have been identified through classical genetic studies. Parallel to genetic approaches, genome-wide approaches have provided valuable insights for the understanding of molecular mechanisms occurring during aging. Gene expression profiling analysis can measure the transcriptional alteration of multiple genes in a genome simultaneously and is widely used to elucidate the mechanisms of complex biological pathways. Here, current global gene expression profiling studies on normal aging and age-related genetic/environmental interventions in widely-used model organisms are briefly reviewed.

• Genomics & Informatics
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• 제17권1호
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• pp.8.1-8.10
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• 2019

Alveolar type II cells constitute a small fraction of the total lung cell mass. However, they play an important role in many cellular processes including trans-differentiation into type I cells as well as repair of lung injury in response to toxic chemicals and respiratory pathogens. Transcription factors are the regulatory proteins dynamically modulating DNA structure and gene expression. Transcription factor profiling in microarray datasets revealed that several members of AP1, ATF, $NF-{\kappa}B$, and C/EBP families involved in diverse responses were expressed in mouse lung type II cells. A transcriptional factor signature consisting of Cebpa, Srebf1, Stat3, Klf5, and Elf3 was identified in lung type II cells, Sox9+ pluripotent lung stem cells as well as in mouse lung development. Identification of the transcription factor profile in mouse lung type II cells will serve as a useful resource and facilitate the integrated analysis of signal transduction pathways and specific gene targets in a variety of physiological conditions.

• Mass Spectrometry Letters
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• 제3권3호
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• pp.74-77
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• 2012

Organic acid (OA) profiling analysis was performed in culture media from Lactobacillus pentosus K34 (L. pentosus K34) and Pediococcus lolli PL24 (P. lolli PL24) by gas chromatography-mass spectrometry (GC-MS) following methoxime/tert-butyldimethylsilyl derivatives. 12 OAs were positively identified in culture media. Most of OA levels from L. pentosus K34 of hetero lactic fermentation were found to be higher when compared with those from P. lolli PL24 of homo lactic fermentation, which may explain different OA metabolism in each strain. In addition, the distorted dodecagonal star patterns were readily distinguishable, and the characteristics of each strain were well represented. The present study demonstrates that the OA metabolic profiling method by GC-MS combined with star pattern recognition is useful for the monitoring study of characteristic OA metabolism in various microorganisms.

• 한국진공학회지
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• 제8권3B호
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• pp.283-289
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• 1999

Multilayer thin film reference materials for the sputter depth profiling analysis are used to calibrate the sputter depth scale by measuring the sputtering rate and to optimize the sputtering conditions for the best depth resolution. Surface analysis group of Korea Research Institute of Standards and science (KRISS) have developed various types of multilayer thin films by using an ion beam sputter deposition and in-situ surface analysis system. The chemical states of the thin films reference materials were certified by in-situ XPS and the thicknesses were certified by transmission electron microscopy (TEM).

• 정보보호학회논문지
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• 제32권6호
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• pp.1045-1057
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• 2022

최근 양자 내성 암호 표준화 사업을 진행 중인 미국의 국립표준기술연구소는 표준화가 확정된 4개의 알고리즘을 발표하였다. 본 논문에서는 PKE/KEM 분야에서 표준화가 확정된 CRYSTALS-KYBER 알고리즘의 복호화 과정 중 비프로파일링 기반 전력 분석 공격인 CPA(Correlation Power Analysis)와 DDLA(Differential Deep Learning Analysis)에 의해 개인 키가 노출될 수 있음을 보이고자 한다. 실험 결과 개인 키의 일차 다항식 계수복구에 성공하였으며, 특히 DDLA에서는 중간 값의 해밍 웨이트(Hamming Weight)를 라벨로 사용하는 모델에서 평가 기법인 NMM(Normalized Maximum Margin)의 값이 13.0으로 가장 높은 값을 가져 개인 키를 복구할 수 있는 것을 확인하였다. 또한, 복호화 과정 중 암호문을 랜덤하게 분할하고 계수별 곱셈 연산의 시작 지점을 랜덤화하는 방어 기법을 적용하면 상기한 공격을 방어하는 것을 확인하였다.

• 한국인터넷방송통신학회논문지
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• 제21권5호
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• pp.127-134
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• 2021

최근 빅데이터 산업의 확대로 고품질의 데이터를 확보하는 것이 중요한 이슈로 떠오르고 있다. 고품질의 데이터를 확보하기 위해서는 데이터에 품질에 대한 정확한 평가가 선행되어야 한다. 데이터의 품질은 데이터에 대한 통계와 같은 메타정보를 통해 평가할 수 있는데 이러한 메타정보를 자동으로 추출하는 기능을 데이터 프로파일링이라고 하다. 지금까지 데이터 프로파일링 소프트웨어는 기존의 데이터 품질 또는 시각화 관련 소프트웨어의 부품이나 추가적인 서비스로 제공되는 것이 일반적이었다. 따라서 프로파일링이 요구되는 다양한 환경에서 직접적으로 사용하기에는 적합하지 않았다. 본 논문에서는 이를 해결하기 위해 마이크로 서비스 아키텍처를 적용하여 다양한 환경에서 서비스가 가능한 데이터 프로파일링 소프트웨어의 개발 결과를 제시한다. 개발된 데이터 프로파일러는 restful API를 통해 데이터의 메타정보에 대한 요청과 응답을 제공하여 사용하기 쉬운 서비스를 제공한다. 또한, 특정 환경에 종속되지 않고 다양한 빅데이터 플랫폼이나 데이터 분석 도구들과 원활한 연계가 가능하다는 장점이 있다.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.243-263
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• 2017

벼 품종의 DNA profiling을 위하여 SSR 마커를 활용하여 한국 벼의 품종판별 기술 확립을 위하여 국내에서 육성된 벼 243개 품종에 대하여 최종 선발된 7개의 SSR 마커(RM21, RM257, HsSSR01-52, RM333, RM580, RM1306, RM157)를 이용하여 판별하였다. 판별용으로 이용된 SSR 마커 7개의 총 대립인자 수는 130개였으며, 대립인자 수의 범위는 10 ~ 32개이었고, 평균 대립인자 수는 18.57이었다. PIC 값은 0.679 (HsSSR01-52) ~ 0.895 (RM333)의 범위이었으며, 평균 PIC 값은 0.774이었다. SSR 마커 7개의 조합으로 6단계의 판별을 통해 총 243개 품종 중 243개 모든 품종의 구별이 가능하였다. 이와 같은 결과, 본 연구에 이용된 SSR 7개 마커는 한국 벼 품종의 판별과 순도유지에 매우 효율적으로 이용할 수 있을 것으로 여겨진다.

• Journal of Applied Biological Chemistry
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• 제64권2호
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• pp.141-151
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• 2021

Insects affect crop harvest yield and quality, making plant response mechanisms to insect herbivores a heavily studied topic. However, analysis of plant responses to honeybees is rare. In this study, comprehensive metabolic profiling of Arabidopsis thaliana exposed to honeybees was performed to investigate which metabolites were changed by the insect. A total of 85 metabolites-including chlorophylls, carotenoids, glucosinolates, policosanols, tocopherols, phytosterols, β-amyrin, amino acids, organic acids, sugars, and starch-were identified using high performance liquid chromatography, gas chromatography-mass spectrometry, and gas chromatography-time-of-flight mass spectrometry. The metabolite profiling analysis of Arabidopsis exposed to honeybees showed higher levels of stress-related metabolites. The levels of glucosinolates (glucoraphanin, 4-methoxyglucobrassicin), policosanols (eicosanol, docosanol, tricosanol, tetracosanol), tocopherols (β-tocopherol, γ-tocopherol), putrescine, lysine, and sugars (arabinose, fructose, glucose, mannitol, mannose, raffinose) in Arabidopsis exposed to honeybees were higher than those in unexposed Arabidopsis. Glucosinolates act as defensive compounds against herbivores; policosanols are components of plant waxes; tocopherols act as an antioxidant; and putrescine, lysine, and sugars contribute to stress regulation. Our results suggest that Arabidopsis perceives honeybees as a stress and changes its metabolites to overcome the stress. This is the first step to determining how Arabidopsis reacts to exposure to honeybees.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1162-1169
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• 2023

16S rRNA short amplicon sequencing-based microbiota profiling has been thought of and suggested as a feasible method to assess food safety. However, even if a comprehensive microbial information can be obtained by microbiota profiling, it would not be necessarily sufficient for all circumstances. To prove this, the feasibility of the most widely used V3-V4 amplicon sequencing method for food safety assessment was examined here. We designed a pathogen (Vibrio parahaemolyticus) contamination and/or V. parahaemolyticus-specific phage treatment model of raw oysters under improper storage temperature and monitored their microbial structure changes. The samples stored at refrigerator temperature (negative control, NC) and those that were stored at room temperature without any treatment (no treatment, NT) were included as control groups. The profiling results revealed that no statistical difference exists between the NT group and the pathogen spiked- and/or phage treated-groups even when the bacterial composition was compared at the possible lowest-rank taxa, family/genus level. In the beta-diversity analysis, all the samples except the NC group formed one distinct cluster. Notably, the samples with pathogen and/or phage addition did not form each cluster even though the enumerated number of V. parahaemolyticus in those samples were extremely different. These discrepant results indicate that the feasibility of 16S rRNA short amplicon sequencing should not be overgeneralized in microbiological safety assessment of food samples, such as raw oyster.

• 지구물리와물리탐사
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• 제2권4호
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• pp.202-208
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• 1999

Chirp sonar 시스템을 이용하여 획득한 광역주파수 음향반사자료의 감쇠특성을 나타내는 음향학적 분류변수를 고안하였다. 분류변수는 퇴적물 물성에 따른 분산효과에 의한 감쇠특성을 나타내며, 음향반사신호의 푸리에 변환을 이용한 unwrapped 위상의 미분치로부터 구하였다. 그리고 각기 다른 퇴적상을 나타내는 지점에서 획득된 음향반사신호의 지질음향모델링을 통하여 퇴적물 물성에 따른 감쇠특성을 평가하였다. 그 결과, 분류변수는 입자크기와 성분에 따른 퇴적물 형태와 해저면 굳기에 따라 값의 차이를 보였으며, 이는 음향자료로부터 직접 해저면을 분류하는데 효과적으로 사용할 수 있다.