• Steel and Composite Structures
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• v.34 no.3
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• pp.333-345
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• 2020

In the past, many efficient profiles have been developed for cold-formed steel (CFS) members by judicious intermediate stiffening of the cross-sections, and they have shown improved structural performance over conventional CFS sections. Most of this research work was based on numerical modelling, thus lacking any experimental evidence of the efficiency of these sections. To fulfill this requirement, experimental studies were conducted in this study, on efficient intermediately stiffened CFS sections in flexure, which will result in easy and simple fabrication. Two series of built-up sections, open sections (OS) and box sections (BS), were fabricated and tested under four-point loading with same cross-sectional area. Test strengths, modes of failure, deformed shapes, load vs. mid-span displacements and geometric imperfections were measured and reported. The design strengths were quantified using North American Standards and Indian Standards for cold-formed steel structures. This study confirmed that efficient profiling of CFS sections can improve both the strength and stiffness performance by up to 90%. Closed sections showed better strength performance whereas open sections showed better stiffness performance.

• Microbiology and Biotechnology Letters
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• v.19 no.2
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• pp.135-139
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• 1991

Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30$^{\circ}C$, 250rpm, 48hour of culture and $(NH_4)_2HPO_4$ as nitrogen source.

• Bulletin of the Korean Chemical Society
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• v.35 no.3
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• pp.845-850
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• 2014

The rapid development of shotgun proteomics is paving the way for extensive proteome profiling, while providing extensive information on various post translational modifications (PTMs) that occur to a proteome of interest. For example, the current phosphoproteomic methods can yield more than 10,000 phosphopeptides identified from a proteome sample. Despite these developments, it remains a challenging issue to pinpoint the true phosphorylation sites, especially when multiple sites are possible for phosphorylation in the peptides. We developed the Phospho-UMC filter, which is a simple method of localizing the site of phosphorylation using unique mass classes (UMCs) information to differentiate phosphopeptides with different phosphorylation sites and increase the confidence in phosphorylation site localization. The method was applied to large scale phosphopeptide profiling data and was demonstrated to be effective in the reducing ambiguity associated with the tandem mass spectrometric data analysis of phosphopeptides.

• YAKHAK HOEJI
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• v.42 no.6
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• pp.627-633
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• 1998

Impurity profiling analysis of methamphetamine seized in Korea was investigated for the evidential and intelligent purpose. Samples were extracted with ethylacetate which contai ns internal standard of dioctylsebacate under basic condition and extracts were analyzed by GC-FID. Ephedrine, chloroephedrine & 1,2-dimethyl-3-phenylaziridine were identified impurities in illicit methamphetamine by GC-MS. These impurities revealed that most of abused methamphetamine in Korea were synthesized from ephedrine as a starting material. For the classification of samples. firstly, 24 impurity peaks were selected after inspection of every peak in 50 samples as the specific markers of impurities. Secondly, corresponding peak retention time and area ratio to the internal standard were calculated and database was created with values of 24 peaks by in-house program. Finally, cluster analysis was attempted with the resultant profiles using the STAR plot, which was based on the Euclidian distance for evaluating similarity among samples. A total of 76 samples were divided into 8 different groups within 90% statistical similarity and inter-batch samples showed similar impurity patterns by this procedure. In conclusion, the analysis of impurities is a suitable index for estimation the common or different origin of methamphetamine sample.

• Geophysics and Geophysical Exploration
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• v.2 no.4
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• pp.202-208
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• 1999

We introduce a new acoustic parameter for the classification of seafloor sediments from chirp sonar acoustic profiling data. The acoustic parameter is defined as a derivative of the unwrapped phase of the Fourier transform of acoustic profiling data. Consequently, it represents the characteristics of attenuation by dissipative dispersion in sediments. And we estimated acoustic properties by geoacoustic modeling using Chirp data obtained from the different sedimentary facies. Our classification results, when compared with the results of analysis of sampled sediments, show that the acoustic parameter discriminates sedimentary facies and bottom hardness. Thus the method in this paper is expected to be an effective means of geoacoustic modeling of the seafloor.

• Genomics & Informatics
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• v.12 no.2
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• pp.50-57
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• 2014

We present a new next-generation sequencing-based method to identify somatic mutations of lung cancer. It is a comprehensive mutation profiling protocol to detect somatic mutations in 30 genes found frequently in lung adenocarcinoma. The total length of the target regions is 107 kb, and a capture assay was designed to cover 99% of it. This method exhibited about 97% mean coverage at $30{\times}$ sequencing depth and 42% average specificity when sequencing of more than 3.25 Gb was carried out for the normal sample. We discovered 513 variations from targeted exome sequencing of lung cancer cells, which is 3.9-fold higher than in the normal sample. The variations in cancer cells included previously reported somatic mutations in the COSMIC database, such as variations in TP53, KRAS, and STK11 of sample H-23 and in EGFR of sample H-1650, especially with more than $1,000{\times}$ coverage. Among the somatic mutations, up to 91% of single nucleotide polymorphisms from the two cancer samples were validated by DNA microarray-based genotyping. Our results demonstrated the feasibility of high-throughput mutation profiling with lung adenocarcinoma samples, and the profiling method can be used as a robust and effective protocol for somatic variant screening.

• BMB Reports
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• v.55 no.3
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• pp.113-124
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• 2022

Single-cell RNA sequencing (scRNA-seq) has greatly advanced our understanding of cellular heterogeneity by profiling individual cell transcriptomes. However, cell dissociation from the tissue structure causes a loss of spatial information, which hinders the identification of intercellular communication networks and global transcriptional patterns present in the tissue architecture. To overcome this limitation, novel transcriptomic platforms that preserve spatial information have been actively developed. Significant achievements in imaging technologies have enabled in situ targeted transcriptomic profiling in single cells at single-molecule resolution. In addition, technologies based on mRNA capture followed by sequencing have made possible profiling of the genome-wide transcriptome at the 55-100 ㎛ resolution. Unfortunately, neither imaging-based technology nor capture-based method elucidates a complete picture of the spatial transcriptome in a tissue. Therefore, addressing specific biological questions requires balancing experimental throughput and spatial resolution, mandating the efforts to develop computational algorithms that are pivotal to circumvent technology-specific limitations. In this review, we focus on the current state-of-the-art spatially resolved transcriptomic technologies, describe their applications in a variety of biological domains, and explore recent discoveries demonstrating their enormous potential in biomedical research. We further highlight novel integrative computational methodologies with other data modalities that provide a framework to derive biological insight into heterogeneous and complex tissue organization.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.6
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• pp.2115-2130
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• 2022

With the development of over-the-top (OTT) services, the demand for content is increasing, and you can easily and conveniently acquire various content in the online environment. As a result, copyrighted content can be easily copied and distributed, resulting in serious copyright infringement. Some special forms of online service providers (OSP) use filtering-based technologies to protect copyrights, but illegal uploaders use methods that bypass traditional filters. Uploading with a title that bypasses the filter cannot use a similar search method to detect illegal content. In this paper, we propose a technique for profiling the Heavy Uploader by normalizing the bypassed content title and efficiently detecting illegal content. First, the word is extracted from the normalized title and converted into a bit-array to detect illegal works. This Bloom Filter method has a characteristic that there are false positives but no false negatives. The false positive rate has a trade-off relationship with processing performance. As the false positive rate increases, the processing performance increases, and when the false positive rate decreases, the processing performance increases. We increased the detection rate by directly comparing the word to the result of increasing the false positive rate of the Bloom Filter. The processing time was also as fast as when the false positive rate was increased. Afterwards, we create a function that includes information about overall piracy and identify clustering-based heavy uploaders. Analyze the behavior of heavy uploaders to find the first uploader and detect the source site.

• Mycobiology
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• v.50 no.2
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• pp.110-120
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• 2022

The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new Ceratorhiza hydrophila strain isolated from the submerged aquatic plant Myriophyllum spicatum. Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as Ceratorhiza hydrophila isolate EG19 and the fungus ID is MK387081. The enzyme analyses showed its ability to produce protease and cellulase enzymes. According to the CSLI standard, the ethyl acetate extract of C. hydrophila showed intermediate antibacterial activity against Streptococcus pneumonia, Micrococcus luteus, and Staphylococcus aureus. Metabolic profiling has been carried out using 700 MHz NMR spectroscopy. Based on the ¹H and ¹H-¹³C heteronuclear single quantum coherence (HSQC) NMR data and NMR databases, 23 compounds have been identified. The identified metabolites include 31% amino acids, 9% sugars, 9% amines, 4% sugar alcohols, and 4% alkaloids. This is the first report for the metabolic characterization of C. hydrophila, which gave preliminary information about the fungus. It is expected that our findings not only will pave the way to other perspectives in enormous applications using C. hydrophila as a new promising source of antimicrobial agents and essential metabolites, but also it will be valuable in the classification and chemotaxonomy of the species.

• Current Optics and Photonics
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• v.8 no.1
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• pp.30-37
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• 2024

Implemented at the Geochang Observatory in South Korea, our slope detection and ranging (SLO-DAR) system features a 508 mm Cassegrain telescope (f /7.8), incorporating two Shack-Hartmann wave-front sensors (WFS) for precise measurements of atmospheric phase distortions, particularly from nearby binary or double stars, utilizing an 8 × 8 grid of sampling points. With an ability to reconstruct eight-layer vertical atmospheric profiles, the system quantifies the refractive index structure function (C_n²) through the crossed-beam method. Adaptable in vertical profiling altitude, ranging from a few hundred meters to several kilometers, contingent on the separation angle of binary stars, the system operates in both wide (2.5 to 12.5 arcminute separation angle) and narrow modes (11 to 15 arcsecond separation angle), covering altitudes from 122.3 to 611.5 meters and 6.1 to 8.3 kilometers, respectively. Initial measurements at the Geochang Observatory indicated C_n² values up to 181.7 meters with a Fried parameter (r₀) of 8.4 centimeters in wide mode and up to 7.8 kilometers with an r₀ of 8.0 centimeters in narrow mode, suggesting similar seeing conditions to the Bohyun Observatory and aligning with a comparable 2014-2015 seeing profiling campaign in South Korea.