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St. John's Wort (Hypericum perforatum) stimulates human osteoblastic MG-63 cell proliferation and attenuates trabecular bone loss induced by ovariectomy

  • You, Mi-kyoung;Kim, Du-Woon;Jeong, Kyu-Shik;Bang, Mi-Ae;Kim, Hwan-Seon;Rhuy, Jin;Kim, Hyeon-A
    • Nutrition Research and Practice
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    • v.9 no.5
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    • pp.459-465
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    • 2015
  • BACKGROUND/OBJECFTIVES: The effect of St. John's Wort extract (SJW) on MG-63 cell proliferation and trabecular bone loss induced by ovariectomy was examined. MATERIALS/METHODS: Proliferation, expression of estrogen receptor (ER) ${\alpha}$ and ER ${\beta}$, and gene expressions of osteoprotegerin (OPG), osteocalcin (OC) and alkaline phosphatase (ALP) were examined in MG-63 cells treated with or without SJW. Ovariectomized rats were treated with SJW at the dose of 100 or 200 mg/kg/day, ${\beta}$-estradiol-3-benzoate (E2), or vehicle only (OVX-C), and sham operated rats were treated with vehicle only (Sham-C). Serum ALP and C-telopeptide (CTX), and femoral trabecular bone loss were examined. RESULTS: SJW increased MG-63 cell proliferation and expression of ER ${\alpha}$ and ER ${\beta}$, and positive effect was shown on gene expressions of ALP, OC and OPG. SJW also showed estrogen like effect on bone associated with slowing down in trabecular bone loss. Histopathology by H&E showed rats treated with SJW displayed denser structure in metaphyseal region of distal femur compared with rats in OVX-C. SJW was shown to reduce serum CTX in OVX rats. CONCLUSION: The present study provides new insight in preventing estrogen deficiency induced bone loss of SJW and possibility for its application in bone health supplement.

Consciousness on Korean Traditional Food and Holiday Food of Elementary School Dietitians in Jeonnam Area (전남지역 초등학교 영양사의 전통음식과 절식에 대한 인식)

  • Lee, Hwa-Ja;Jung, Lan-Hee
    • Journal of Korean Home Economics Education Association
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    • v.20 no.3
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    • pp.17-30
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    • 2008
  • The purpose of this study was to investigate consciousness on Korean traditional food and holiday foods of the elementary school dietitians, to provide the basic data for positive utilization of the traditional food and holiday foods in school food service. The survey was conducted from 239 elementary school dietitians in Jeonnam area. The used questionnaire consists of 7 questions on dietitians' general information, 3 questions on the importance of Korean traditional food, 6 questions on the succession and development of education on the traditional food. Data were analyzed by a SAS program and frequency, percentage, mean and standard deviation were calculated from it. According to the survey, the preference of the tradition food was remarkable to the dietitians who were elderly and had worked for a long term. The degree of utilization about Korean traditional food was investigated once a month. Education of Korean traditional food intake was generally executed every 6 months. Then the way for that was utilized school home page or school bulletin board. Dietitians reply positively about providing Korean traditional food for the school food service. The reasons were the followings. to be able to flavor a taste of the Korean traditional food, to hand down Korean dietary customs and to help keep good health.

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Anti-melanogenic effects of Hordeum vulgare L. barely sprout extract in murine B16F10 melanoma cells

  • Choi, Jeong-Hwa;Jung, Jong-Gi;Kim, Jung-Eun;Bang, Mi-Ae
    • Journal of Nutrition and Health
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    • v.52 no.2
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    • pp.168-175
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    • 2019
  • Purpose: Barely sprout is a well-known oriental herbal medicine with a wide range of health benefits. Recent studies have provided scientific evidence of its therapeutic effects with expanded application. This study investigated anti-melanogenic effect of barley sprout water extract (BSE) in murine melanocyte B16F10. Methods: Various concentrations (0, 50, 125, and $250{\mu}g/mL$) of BSE and arbutin (150 ppm) were applied to B16F10 stimulated with or without alpha-melanocyte stimulating hormone (100 nM) for 72 hours. The whitening potency of BSE was determined altered cellular melanin contents. Activity and expression of tyrosinase and microphthalmia-associated transcription factor (MITF) were also assayed. Results: Experimental results revealed that treatment with BSE reduced cellular melanin production by approximately 40% compared to the control. Molecular findings supported that suppressed activity and expression of tyrosinase and MITF proteins by BSE were associated with declined cellular melanogenesis. Furthermore, anti-melanogenic effect of BSE ($250{\mu}g/mL$) was similar to that of arbutin, a commonly used whitening agent. Lastly, polyphenols including p-coumaric, ferulic, and vanillic acids were identified in BSE using HPLC analyses. They might be potential active ingredients showing such melanogenesis-reducing effect. Conclusion: BSE was evident to possess favorable anti-melanogenic potency in an in vitro model. As a natural food sourced material, BSE could be an effective depigmentation agent with potential application in pharmaceutical and cosmetic industries.

Resoures Plants Distributed in the Warm-temperature Zone of Jeonnam Province (전남지방에 분포하는 난대 자원식물 - 희귀특산 목본식물을 중심으로 -)

  • You, Han-Choon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.23-23
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    • 2019
  • There are many island areas in Jeonnam Province and various plants are naturally grown due to their regional characteristics. Among them 217species were found to be Rare-Specialty plants in Jonnam Province. There are growing 35 species of Critically Endangered(CR), 37 species of Endangered Species(EN), 54 species of Vulnerable(VU) and 36 species of Least Concern(LC), many of which are native to islands far south or western of the sea. This report briefly describes the geographical distribution location and the state of reproduction among Rare-Specialty woody plants grown in the Warm-temperature Zone of Jeonnam Province. There are growing Michella compressa(Shinan-gun), Actinidia rufa(Wando-gun), Glochidion chodoense(Jindo-gun), Rhus ambigua(Yeosu), Wikstroemia ganpi (Goheung-gun, Yeosu) among of the 35 Critically Endangered species. Woody plants of Endangered Species(EN) are growing Albizia kalkora(Mokpo, Muan-gun), Daphne kiusiana(Shinan-gun), Marsdenia tomentosa(Wando-gun), Osmanthus insuralis(Yeosu, Shinan-gun). Woody plants of Vulnerable(VU) are growing Ardisia crenata(Wando-gun, Shinan-gun), Hibiscus hamabo(Wando-gun), Illicium anisatum (Jindo-gun), Koelreuteria paniculata(Wando-gun), Wikstroemia ganpi(Youngam-gun). Woody plants of Least Concern(LC) are Celtis choseniana(Yeosu), Chionanthus retusus(Wando-gun, Goheung-gun), Cinnamomum camphora(Wando-gun, Jindo-gun), Linder sericea(Boseong-gun).

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Development of a Simultaneous Analytical Method for Diquat, Paraquat and Chlormequat in Animal Products Using UPLC-MS/MS

  • Cho, Il Kyu;Rahman, Md. Musfiqur;Seol, Jae Ung;Noh, Hyun Ho;Jo, Hyeong-Wook;Moon, Joon-Kwan
    • Korean Journal of Environmental Agriculture
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    • v.39 no.4
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    • pp.368-374
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    • 2020
  • BACKGROUND: The residual analysis of polar pesticides has remained a challenge. It is even more difficult to simultaneously analyze multiple polar pesticides. Diquat, paraquat, and chlormequat are typical examples of highly polar pesticides. The existing methods for the analysis of diquat, paraquat and chlormequat are complex and time consuming. Therefore, a simple, quick and effective method was developed in the represent study for simultaneous analysis of diquat, paraquat and chlormequat in animal products, meat and fat using UPLC-MS/MS. METHODS AND RESULTS: Sample extraction was carried out using acidified acetonitrile and water and re- extracted with acidified acetonitrile and combine the extracts followed by centrifugation. The extract was then cleaned up with a HLB cartridge after reconstitution with acidic acetonitrile and water. The method was validated in quintuplicate at three different concentrations. The limits of detection (LOD) and quantification (LOQ) were 0.0015 and 0.005 mg/L, respectively. Matrix suppression effect was observed for all of the analytes. A seven point matrix matched calibration curve was constructed for each of the compound resulted excellent linearity with determination coefficients (R2) ≥ 0.991. Accuracy and precision of the method was calculated from the recovery and repeatability and ranged from 62.4 to 119.7% with relative standard deviation less than 18.8%. CONCLUSION: The recovery and repeatability of the developed method were in the acceptable range according to the Codex Alimentarius guideline. The developed method can be applied for the routine monitoring of diquat, paraquat, and chlormequat in animal products, meat and fat.

Clinical and Serologic Study of Herpes Zoster in Children and Adolescents in Gwangju-Jeonnam Province (광주·전남 지역의 소아·청소년 대상포진에 대한 임상적 비교 및 혈청학적 의의에 대한 연구)

  • Na, Chan Ho;Kim, Choong Jae;Shin, Bong Seok;Choi, Hoon;Kim, Min Sung
    • Korean journal of dermatology
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    • v.56 no.9
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    • pp.531-538
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    • 2018
  • Background: Herpes zoster is a common dermatologic disorder. However, it rarely occurs in children and adolescents in Gwangju-Jeonnam province. Objective: This study aimed to analyze the epidemiological features and clinical characteristics of herpes zoster in children and adolescents. Methods: A retrospective survey was conducted on patients who visited the department of dermatology of Chosun University Hospital in Gwangju-Jeonnam province within the past 8 years. The medical records of 103 patients aged <18 years were reviewed. We analyzed for age, gender, accompanying symptoms, dermatomal distribution, underlying disease, treatment, and complications with serologic test. Results: The male-to-female ratio of the participants was 1.08:1, and their mean age was 13.0 years. Underlying diseases were observed in 3% (3/102) of the patients. The most common dermatomal distribution was thoracic dermatome (34%), followed by trigeminal (26%), cervical (20%), lumbar (15%), and non-skin (6%). The most common accompanying symptoms were headache (10%), fever (3%), and myalgia (3%). No difference was observed between patients who were varicella-zoster virus (VZV) IgM-positive and those who were VZV IgM-negative in terms of dermatome, visual analogue scale (VAS), severity, and body mass index (BMI). Conclusion: In children and adolescents with herpes zoster, the gender ratio and dermatomal distribution were similar to those previously reported, except for the low rate of underlying diseases. The incidence of herpes zoster in children was not significantly associated with immunosuppression and underlying diseases. Higher VZV IgM titer was not associated with dermatomal distribution, higher VAS score, or BMI. This study first compared the serological test results of children.

Studies on the Ecology and Control of Bog Pondweed(Potamogeton distinctus A. BENN) in Paddy Field (답숙근초 가래의 생태와 방제에 관한 연구)

  • Lee, H.K.;Park, H.C.;Lee, D.K.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.21 no.2
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    • pp.258-268
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    • 1976
  • This study was conducted in order to know the ecology and to establish the control method of Potamogeton distinctus A. BENN. The tubers of Bog pond weed was almostly located at soil depth of 10 cm to 20cm, germination rates and number of formed tubers were different according to transplanting depth of soil. Effects of herbicides application on weeds were different according to leaf-stage of pondweed, and the pondweed were withered to death by absorption of Avirosan into the leaves. It was suitable to keep water depth at 3cm level until about a week after Avirosan application.

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Occurrence Patterns of Paddy Weeds and Distribution of Resistant Weeds to an ALS Inhibiting Herbicide in Jeonnam by a Soil Assay Method (토양검정법에 의한 전남지역 논잡초 발생양상과 ALS 저해제 제초제 저항성 논잡초 분포)

  • Jeong, Jang Yong;Yun, Young Beom;Jang, Se Ji;Hyun, Kyu Hwn;Shin, Dong Young;Lee, Jeongran;Kwon, Oh Do;Kuk, Yong In
    • Weed & Turfgrass Science
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    • v.7 no.3
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    • pp.191-199
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    • 2018
  • This study was to investigate the occurrence patterns of paddy weeds, their resistance levels to an ALS inhibiting herbicide, and to estimate the areas of resistance in these paddy fields. We used soil collected from 358 paddy fields of Jeonnam province in 2017. Based on their life cycles, weeds were 96% annuals and 4% perennial. Additionally, according to morphological classification, 59% were broad leaves, 28% were sedges and 13% were grasses. Different areas within Jeonnam province contained different numbers and occurrence rates of weed species. However, generally, we observed Lindernia dubia var. dubia, Monochoria vaginalis var. plantaginea, Ludwigia prostrata, L. procumbens, Cyperus difformis, Scirpus juncoides, Eleocharis Kuroguwai, Echinochloa oryzoides, and E. crus-galli var. echinata. We also observed seven weeds resistant to an ALS inhibiting herbicide. They were M. vaginalis, S. juncoides, C. difformis, L. dubia, Ludwigia prostrata, E. oryzoides, and E. crus-galli var. echinata. Although there were differences in the number and occurrence rate of resistant weed species to an ALS inhibiting herbicide among areas in Jeonnam province, the M. vaginalis, C. difformis, and S. juncoides occurred in 23 cities and counties in Jeonnam including Gwangju metropolitan city. Based on the rates (52%) of resistant occurrence to an ALS inhibiting herbicide in Jeonnam province, the area of weed resistant paddy fields was estimated to be 91,543 ha.

The Dietary Habits and Perception of Vegetable Intake of Elementary Students in Gwangju and Jeonnam (광주·전남 일부지역 초등학생의 채소류 섭취에 대한 인식)

  • Go, Young-Sook;Jeon, Eun-Raye;Jung, Lan-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.2
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    • pp.223-233
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    • 2013
  • The purpose of this study was to analyze the dietary habits and perception of vegetable intake of elementary students in Gwangju and Jeonnam Gokseong county. Data collection was conducted from 5th and 6th grade students of elementary schools in Gwangju and Jeonnam Gokseong county using a structured questionnaire survey. The SPSS program was used for statistics processing and data analysis. The chi-square test was also conducted. In terms of dietary intake habits, female students consumed their meals slower than male students. Information on dietary habits and nutrition was commonly obtained from family, including the mother or father who commonly prepared meals at home. Snacks were commonly consumed less than twice daily, with the Gwangju area having a higher frequency of snacks than the Jeonnam area. Elementary students indicated that vegetables were their least favorite food, with female students having a higher interest in vegetables than male students. The pattern and perception of vegetable intake came when the students (that did not eat vegetables) were lectured by their parents on the nutritive value of vegetables. Most students understood the important nutritional ingredients of vegetables. In the case of an interest in vegetables, the Gwangju area showed significantly more comprehension than the Jeonnam area on the definition and role of dietary fiber, the dental benefits of dietary fiber, and the identification of the environment-friendly certification mark.

Characteristic of mycelial growth of cauliflower mushroom (Sparassis latifolia) using replacement culture with Trichoderma and rDNA analysis in genealogy of crossbreeding strain (푸른곰팡이 대치배양에 의한 꽃송이버섯 균사 생장 특성 및 계통간 교잡균주의 rDNA 분석)

  • Oh, Deuk-Sil;Kim, Hyun-Suk;Kim, Young;Wi, An-Jin;Yoon, Byung-Sun;Park, Whoa-Shig;Park, Hyeong-Ho;Wang, Seung-Jin
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.41-51
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    • 2014
  • Cauliflower mushroom widely known high concent of ${\beta}$-glucan for farm cultivation invigoration verified characteristics of mycelia growth, genetic diversity, resistance to Trichoderma by replacement culture with Trichoderma and growth characteristics of new variety crossbleeding strain. The result of replacement culture with Trichoderma for verification resistance about Trichoderma, 6951 (T. viride) strain did not show special change after formation of confrontation line and 6952 (T. spp.) strain was showed more formation of spore after formation of confrontation line. But 6426 (T. harzianum) strain found to encroach part of growth area of cauliflower mushroom mycelia. Among 10 kinds cauliflower mushroom strain, JF02-06 strain collected by Gurye, found did not spore of Trichoderma and thought to be resistant to Trichoderma. The result of crossbleeding after selected that mother strain good growth and formation of fruit body, verified good mycelia growth at JF02-47, 49 and 50 strain in Korean pine of wood-chip media. The result of gene sequence about ITS1, 5.8S and ITS4 for analysis of genetic diversity at crossbleeding strain, found high significance to other cauliflower mushroom in registered Genebank. The result of growth characteristic of spore and mycelia of cauliflower mushroom by observation microscope, size of spore showed water drop shape to major axis $6{\mu}m$ and minor axis $5{\mu}m$ and clamp showed 3 types in mycelia. The wide of mycelia was $3{\mu}m$. The characteristic of mycelia of cauliflower mushroom found to grow mycelia in clamp at approximately 50%. The growth speed of mycelia was $0.507{\mu}m/min$ and 2nd mycelia grown similar speed to mother mycelia at parallel with mother mycelia after growth speed at $0.082{\mu}m/min$. The formation of clamp made small clamp for 5 hours after shown transfer of electrolyte in mycelia inside. The septum formation started after 3 hours and then finally completed after 2 hours. In this study, strain of cauliflower mushroom verified resistance of Trichoderma, genetic diversity and characteristic of mycelia growth. Therefore, basic knowledge of cauliflower mushroom will improve and further contribute to development of mushroom industry.