• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.595-599
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• 2011

'Goami 3' is a new japonica rice cultivar developed from a cross between Suweon464 and Daeanbyeo by the rice breeding team of National Institute of Crop Science, RDA. 'Goami3' has about 130 days growth duration from transplanting to heading in central plain area of Korea. It has a good semi-erect plant type and resistance to lodging of about 79 cm in culm length. 'Goami 3' had 15 panicles per hill and 104 spikelets per panicle. 'Goami 3' has very high amylose (29.5%) and high non-dietary starch compared with Hwaseongbyeo. This rice variety has slow senescence and 47% tolerance to viviparous germination during the ripening stage. 'Goami 3' is susceptible to leaf blast, bacterial blight, virus disease and insect pest. The yield performance of this cultivar in milled rice was about 3.92MT/ha by ordinary season culture in local adaptability test from 2005 to 2007. 'Goami 3' is adaptable to central and southern plain area of Korea.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.257-261
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• 2010

'Manmibyeo', a new japonica rice cultivar, is a mid-late maturing ecotype cultivar developed by the rice breeding team of Department of Functional Crop, NICS, RDA in 2002. This cultivar was originated from the cross between 'Milyang 95' and $F_1$ of the cross between 'Bukrukbanna' and 'Milyang 95' in 1990/1991 winter season. It was selected by pedigree breeding method until F6 generation, and a promising line, YR14545-9-2-3-4, was advanced and designated as 'Milyang 162' in 1996. The local adaptability test of 'Milyang 162' was carried out at seven locations during 3 years in 1997, 1998 and 2002. It has about 86cm culm length and is lodging tolerant. This cultivar is susceptible to bacterial blight ($K_1$, $K_2$, $K_3$) and stripe virus, and moderately resistant to leaf blast disease. Milled rice kernels of 'Manmibyeo' is translucent with low amylose content in endosperm, clear in chalkiness and good at eating quality in panel test. The yield potential of 'Manmibyeo' in milled rice was about 4.46 MT/ha at ordinary fertilizer level in local adaptability test. This cultivar would be adaptable to the southern plain of Korea.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.262-266
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• 2010

'Josaengheugchal', a new blackish purple pigmented glutinous japonica rice cultivar, was developed by the rice breeding team of Department of Functional Crop, NICS, RDA in 2004. This cultivar was derived from a cross between 'Tohoku 149' as black glutinous source and 'Sx 864' as purple colored rice in 1992 and 1993 winter season, and selected by pedigree breeding method until $F_6$ generation. As a result, a promising line, YR15907-6-8-1-5, was advanced and designated as the name of 'Milyang 194' in 2001. The local adaptability test of 'Milyang 194' was carried out at seven locations from 2002 to 2004 and it was named as 'Josaengheugchal'. 'Josaengheugchal' is an early maturing cultivar and has 71 cm culm height. It has higher anthocyanian content compared with 'Heugnambyeo'. It is moderately resistant to leaf blast but susceptible to other disease and insect pests. The yield potential of 'Josaengheugchal' in brown rice was about 4.21 MT/ha at ordinary fertilizer level in local adaptability test. This cultivar would be adaptable to the plain paddy field of middle, Honam, and Yeomgnam in Korea under ordinary and double cropping system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.1
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• pp.11-24
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• 1979

To characterize elastic and plastic chilling injury, rice seedlings grown at 28/$16^{\circ}C$ day/night temp. under 20K lux (13hrs.) in a phytotron were subjected to a 11/$6^{\circ}C$, 20K lux condition for 2, 4, 6 or 8 days at 1, 2, 3, 4 or 5th leaf-stage, respectively, followed by further growth under 28/$16^{\circ}C$condition till 30th day after seeding. Japonica variety Jinheung and Chulwon No.1 survived almost 100% without any significant , discoloration and death of leaves due to chilling even under the chilling of 8 days at all seedling ages tested. Tongil and Yushin, varieties from Indica x Japonica cross, showed increasing discoloration of leaves and death of plants with increase in chilling intensity. The longest chilling duration shown seedling death less than 5% was 4, 6, 1, 4, 8 days for Tongil, and 6, 6, 1, 2, 2, days for Yushin at 1, 2, 3, 5th leaf-stage, respectively. The degree of discoloration and death of leaves or suppression of height growth was not explicitly related to seedling death or the dry weight reduction. The degree of seedling death or dry weight reduction could differentiate chilling tolerance of varieties and seedling ages, but somewhat differently. Reduction in dry weight due to chilling occurred even without any visible injury or seedling death. These suggest that both the degree of seedling death and reduction in dry weight should be considered in the test of varieties for chilling tolerance. Combined evaluation of seedling death and dry weight reduction indicated the most susceptible seedling age to chilling injury to be 1 to 2nd leaf-stage for Jinheung, 2 to 3rd leaf-stage for Chulwon No.1, 3rd leaf- stage for Tongil and Yushin, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.1-10
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• 1982

Degree of grain shattering which is of varietal character is an important determinant for the magnitude of field loss of grains during harvest and threshing. Seven Indica \times Japonica progeny varieties and four Japonica varieties were subjected to measurements of tensile strength of grains, degree of grain shattering when panicles were dropped at 1.5m above concrete floor, and moisture content of grains (wet basis) during a period 35 to 63 days after heading. In addition, two varieties were tested for the relation of tensile strength of grains to the magnitude of field loss of grains in actual binder harvest. The 11 varieties differed conspicuously in tensile strength of grains and the degree of grain shattering: the weakest average tensile strength of grains of a variety was about 90g and the strongest about 250g with varying standard deviation of 30 to 60g. Three Indica \times Japonica varieties and one Japonica variety shattered I to 30% of the grains under the falling test. The threshold tensile strength of grains allowing grain shattering was estimated to be 180g on average for a sampling unit of 10 panicles, but only the grains having tensile strength weaker than 98g within the samples shattered. A decrease in average tensile strength by 10g below the threshold value corresponded to an increase of 3 to 5% in grain shattering. Most varieties did not change appreciably the tensile strength of grains and degree of grain shattering with delay in time of harvest and showed a negative correlation between the tensile strength and the moisture content of grains. The average tensile strength of grains was negatively correlated linearly with field loss in binder harvest. The average tensile strength for zero field loss in binder harvest was estimated to be 174g and a decrease in the average tensile strength by 10g corresponded to an increase of 40kg per hectare in field loss of grains. Instead of the average tensile strength of grains, the percentage of grains having tensile strength weaker than 100g is recommended as a criterion for the estimation of field loss of grains during harvesting operations as well as a basis of variety classification for grain shattering, since the standard deviation of tensile strength of grains varies much with variety and time of harvest, and individual grains having tensile strength stronger than 98 did not shatter practically.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.13
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• pp.1-40
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• 1973

These studies were aimed at clarification of genetic and ecological variation in culm length, panicle length and plant height of the $\textrm{F}_2$ plants in some selected crosses made between semi-dwarf rice varieties and tall Japonica ones. One Indica semi-dwarf, Taichung Native 1, one Indica $\times$ Japonica hybrid, IE51 and one Japonica semi-dwarf, Tankanbaekmang were used as short-gene donors while two of medium maturity varieties, Jinheung and Kwanok and one late veriety, Palkweng were used as the corresponding counterpart of respective dwarf varieties in a series of crosses. Five different crosses, Kwanok $\times$ Tankanbaekmang, Palkweng $\times$ Tankanbaekmang, Jinheung $\times$ T(N)1, Kwanok $\times$ T(N)1 and Kwanok $\times$ IE51, were made among the above six varieties. The $\textrm{F}_2$ plants of these crosses together with the concerned parental varieties were grown under several different conditions including three levels of each nitrogen and planting space, three planting seasons and three locations in 1968, to investigate variation in length of culm and panicle, and plant height. On the other hand, the F$_3$ progenies which were derived from the shortest 10 percent of the plants of three $\textrm{F}_2$ populations, Kwanok $\times$ T(N)1, Jinheung $\times$ T(N) 1 and Kwanok $\times$ IE51 grown in the previous year, were compared each other on the basis of selection efficiency in culm length. The experimental results could be summarized as follows; 1. Genetic behavior A. It was revealed that Tankanbaekmang, one of Japonica dwarf has a simple recessive gene responsible for short culm expression, showing a typical segregation ratio of three tall to one short culm plants in $\textrm{F}_2$ generation of the crosses either with Kwanok or Palkweng. B. In the both combinations, segregation pattern of the panicle length was exactly same as that of culm length. It seems that the same gene controls both culm length and panicle length. C. No difference between segregation of culm length and plant height in the above crosses was observed. D. T(N)1, one of Indica semi-dwarf did not show such a simple genetic behavior as detected from the crosses with Tankanbaekmang in segregation of culm length but formed a continuous and normal distribution curve. Therefore, some nonallelic genic actions might be involved in expression of culm length of the counterpart varieties of T(N)1. In particular, a transgressive segregation appeared toward the direction of longer culm length in case of Jinheung $\times$ T(N)1. The genetic behavior of panicle length and plant height generally coincided with that of culm length in all the cases. E. IE51 demonstrated exactly the same genetic behavior as that of T(N)1 when this variety was crossed with Kwanok. It was clearly clarified that the simple recessive gene controlling dwarfism from T(N)1 was well incorporated into this variety. 2. Ecological variation A. In general, there was a decreasing tendency in culm length and plant height of rice plant as seeding delayed while it was not so noticeable in panicle length. The decreasing magnitude varied from variety to variety and from cross to cross. Genetic behavior of the culm length and related characters of these materials was not disturbed by the variation of seeding season, nitrogen level, planting space and experimental location. E. The elongation mode of the upper three internodes was very similar to the segregation mode of culm length, panicle length and plant height in $\textrm{F}_2$ populations of . all the crosses investigated in this study. Accordingly, this result confirmed that the roles of the upper three internodes are very important in manifesting plant stature in rice. C. The effect of nitrogen on culm length and the related other two characters seemed to be meager. However, it was true to show an increasing tendency of those characters as nitrogen level got increased from 4 kg to 12kg per l0a, with different magnitude depending upon variety or cross. D. Also, the effect of planting space on culm length, panicle length and plant height was relatively small in all the cases. Those characters varied again depending upon variety or cross. However, a general increasing tendency was detected in manifestation of those traits under denser planting space condition. E. All the parental varieties produced shorter culm, panicle and plant height when they were grown at the lower latitude locations. It might be attributed to the fact that their reproductive growth accelerated with increased temperature prevailing at the lower latitude locations such as Iri and Mi1yang. On the countrary, $\textrm{F}_2$ population reacted differently to the different locations from the parental varieties. All the $\textrm{F}_2$ plants produced the longest culm, panicle and plant at Milyang. 3. Selection efficiency A. The heritability of culm length in Kwanok $\times$ T(N)1, Kwanok $\times$ IE51 and Jinheung$\times$T(N)1 was 92 percent, 74 percent and 55 percent, respectively. B. The actual genetic advance for culm length obtained from the progeny lines of the selected plants(10 precent) from the $\textrm{F}_2$ generation, was comparable to the expected advance calculated from the original $\textrm{F}_2$ populations. As compared with the $\textrm{F}_2$ population, the $\textrm{F}_3$ plants of Kwanok $\times$ T(N)l shortened on the average by 20.8cm, those of Kwanok $\times$ IE51 did 8.7cm and those of Jinheung$\times$T(N)1 20.0cm, respectively. C. Panicle length of the populations was differently affected from one cross to another by the selection based upon culm length in $\textrm{F}_2$ Kwanok $\times$ T(N)1 did not show any noticeable shortening of its culm length due to the selection pressure. On the other hand, both Kwanok $\times$ IE51 and Jinheung $\times$ T(N)1 showed a considerable shortening of their panicles in case of selection for culm length. Based upon the above results, it could be concluded that the ecological variation in culm length, panicle length and plant height was relatively small and fallen within the range of genetic variation. Considering from the fact that the simple recessive gene governing short height of Tankanbaekmang always accompanied with some undesirable characters such as short panicle and extremely small grain, the short gene of T(N)1 seemed to be more useful as dwarf gene source since it did not carry short gene together with such undesirable traits.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.13-19
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• 1998

We have reported previously that intact embryogenic cells can be used instead of protoplasts for electroporation-mediated transformation of zoysiagrass and rice. In this study, conditions of the tissue electroporation were examined to optimize the procedures. Embryogenic cell suspensions were established in liquid MS medium containing 2 mg/L of 2,4-D with embryogenic calluses induced from mature embryos of Z. japonica. The suspension-cultured cell clumps were electroporated with 35S-gusA expression vector DNA, and degrees of DNA introduction into the cells were determined by histological expression rates of the gusA marker gene. DNA transfer into the cell clumps occurred in wide range of voltage (100-400 V) and capacitance (10-1980 $\mu\textrm{F}$), but more in the ranges of 200-300 V and 330-800 $\mu\textrm{F}$ DNA concentrations higher than 6 $\mu\textrm{g}$/mL were adequate for GUS expression of the electroporated cells. DNA transfers were confirmed in all three embryogenic cell lines but only in one out of eleven non-embryogenic lines. Positive GUS expressions occurred with DNAs added even 20-40 h after pulse treatments. As a promoter of gusA, Act1 and Ubi1 were effective 7 and 5 times than 35S respectively in number of GUS expression units on electroporated cell clumps. Embryogenic cell clumps survived and regenerated into plantlets after pulse treatments of wide range of conditions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.3
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• pp.309-314
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• 1988

This study was carried out to get information for the breeding varieties and developing the cultural methods with representative 32 cultivars which were diffused from 1900 to 1982 and two cultural methods which were old one i.e., low dressing and late planting and modern one i.e., medium dressing and early planting. Duration of transplanting between cultural practices were 15 days but the duration of heading date were 5 days. Panicle projecting duration was 5-10 days in all cultivars, especially, it is 5 days in early maturing varieties, 6-7 days in medium maturing varieties and 7-10 days in late maturing varieties. Periods from transplanting date to effective ripening date were 99.8 days in low dressing and late planting method, 106.2 days in modern one but the periods from heading date to effective ripening date were 31.4 days in low dressing and late planting method, 30.2 days in modern one. Ripening speed was very active from 5 to 10 days after heading and the daily increase of 1000 grain weight was from 847.8mg to 1130mg in that time and it slightly increased from 5 to 35 days after heading in japonica varieties but increased after heading and suddenly decreased at 30 days after heading in Indica/Japonica varieties. Meteorological factors (temperature and amount of solar radiation) were positively correlated with the ripening speed but negatively correlated with the period of physiological and effective ripening.

• Korean Journal Plant Pathology
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• v.3 no.3
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• pp.174-179
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• 1987

Four thousand eight hundred and eighty five isolates of Pyricularia oryzae were obtained from the diseased rice specimens collected from various areas of Korea for race identification during 1978 - 1985. A total of 15 races inculding 6 races pathogenic to Tongil lines, 2 T-races, 5 C-races and 2 N-races was identified using a old Japanese differential set during 1978-1980. Since 1981, number of races identified by a Korean differential set was 18 races which were composed of 11 KI-races pathogenic to either Tongil lines or Japonica cultivars and 8 KJ-races pathogenic to only Japonica cultivars. The prevalent race was $N-2^{+t}$ during 1978-1979 and race KJ-301 since 1980, respectively. Races KI-315a and KI-315b pathogenic to most of Tongil lines were identified in 1983. being widely distributed to date. Races KJ-105 and KJ-201 pathogenic to the cultivars possessing resistance genes Pi-k and Pi-i were prevalent in Gangweon province, whereas race KI-315b was prevalent in Chungbug and Jeonnam districts.

• Journal of Life Science
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• v.21 no.11
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• pp.1619-1624
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• 2011

Endophytic fungal strains were isolated from the roots of six species plants in the Dokdo islands. Native plant samples, such as Artemisia japonica, Chenopodium album and Solanum nigrum were isolated from Dongdo, and those such as Cyrtomium falcatum, Dianthus longicalyx and Tetragonia tetragonoides were isolated from Seodo. In total, thirty two fungal strains were isolated from these native plants. To identify the fungal strains, polymerase chain reaction (PCR) amplification of internal transcribed spacer (ITS: containing ITS1, 5.8s and ITS2 region) regions was done with universal primers ITS1 and ITS4. Endophytic fungi of four species were isolated from A. japonica, eight species from C. album, three species from S. nigrum, three species from C. falcatum, three species from D. longicalyx and eleven species from T. tetragonoides. Culture filtrates (CF) of isolated endophytic fungi were used to treatwaito-c rice seedlings to test plant growth-promoting activity. As a result of bioassay, Ca-5-2-2 strain isolated from C. album expressed highest plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp., Fusarium sp. and Aspergillus sp. were the most abundantly distributed fungal strains in the six plants used in this study.