• Title/Summary/Keyword: italian ryegrass

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Effects of Medium Supplements on Seed-derived Callus Culture of Italian Ryegrass (배지첨가물질이 이탈리안 라이그래스의 종자유래 캘러스 배양에 미치는 영향)

  • Woo, H.S.;Lee, B.H.
    • Journal of Animal Science and Technology
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    • v.46 no.2
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    • pp.243-250
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    • 2004
  • In an effort to optimize tissue culture responses of Italian ryegrass(Lolium multiflorum Lam.) for future genetic manipulations to improve forage characteristics, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of three cultivars, 'Jeanne', 'Florida-80' and 'Metro', as explant tissues. For all explants, MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The optimal concentration of dicamba for the induction of embryogenic callus from mature seeds was 7mg/L. The highest plant regeneration frequency was observed when embryogenic callus was transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Plant regeneration frequency of callus cultured in the dark was higher than that of cultured in the light. Casein hydrolysate and L-proline improved both in embryogenic callus induction from mature seeds and plant regeneration. High-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

Profitability Analysis Based on Cropping System of the Land Use Changes in Paddy Field (논 타작물 재배 작부체계별 수익성 분석)

  • Yu, Chan-Ju;Um, Ji-Bum;Ko, Hyeon-Seok;Park, Sang-Youn
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.1
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    • pp.216-222
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    • 2020
  • A comparative analysis of farm incomes was performed from the new composition system of crops, such as feed crops, including soybeans, Italian ryegrass, soybean-potatoes, and soybean-barley planted in rice fields. Through this analysis, the suggested implications were derived, and the technological solutions were determined. The profitability of the composition of soybean-Italian ryegrass showed that the soybeans yield was 325kg/10a, and the sale price was ₩3,962/kg, leading to a gross revenue of ₩1,288,000/10a. The yield of Italian ryegrass was 1,584kg/10a with a sale price of ₩125/kg, showing a gross revenue of ₩198,000/10a. The net income of soybeans was ₩284,000/10a, which means a net income rate of 22.1%, whereas the net income of Italian ryegrass was -₩30,000/10a. The composition of soybeans-potatoes and soybeans-barley showed a gross revenue of ₩930,000/10a and ₩375,000/10a, respectively. The crop composition types have been developed for the Income Type, Production Type of Forage, Improvement Type of Food Self-Sufficiency, and Corresponding Type to Climate Change. On the other hand, regional types have not been developed sufficiently. In addition, various selections of alternative crops and the establishment of crop composition congruent to the specific regions should be developed systematically in terms of the production technology. Therefore, it is necessary to develop the technological establishment of the crop composition through continuous related studies with the mid-to-long term objectives of land utilization and grain self-sufficiency rates.

Effect of Moisture Content on Physical and Chemical Characteristics of Italian Ryegrass Cube (수분 함량이 이탈리안 라이그라스 큐브의 물리적 및 화학적 성상에 미치는 영향)

  • Moon, Byeong Heoun;Park, Hyung Soo;Shin, Jong Seo;Park, Byeong Ki;Kim, Jong Geun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.1
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    • pp.34-40
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    • 2016
  • The objective of this study was to determine the effect of moisture content on the physical and chemical characteristics of Italian ryegrass cube. Cube quality according to moisture contents (15, 20, 25, and 30%) was determined. Cubes made with 15 to 20% moisture showed a little cracks. But, the amount of powder generate from these cubes were lower by 10 to 16% compared to other cubes made with 25 to 30% moisture contents. The highest hardness at 159 kg/f was obtained when the cube was made with 15% moisture content and the lowest was 70 kg/f when the cube was made with 30% moisture content. The electrical loading and surface temperature were increased when moisture content was decreased. The chemical compositions of cube were differ from those of raw materials. Crude protein (CP) and ether extract (EE) contents were increased after cubing works. However, crude fiber (CF), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents were decreased after cubing. The crude ash content was not significantly (p > 0.05) different between raw material and cube. Higher moisture content resulted in higher crude protein content. However, crude fiber and crude ash content were not significantly (p > 0.05) different between each other. The contents of ADF and NDF were the lowest in cubes made with 30% moisture content. Our results suggest that the proper moisture content of Italian ryegrass cubing is recommended to be 15 to 20% and that cubing works should help increase forage quality.

Plant Regeneration From Mature Seed of Domestic Italian Ryegrass Cultivar (국내개발 이탈리안 라이그라스 품종 성숙종자의 식물체 재분화)

  • Kim, Kyung-Hee;Kim, Yong-Goo;Heo, Sung-Hyun;Lee, Ki-Won;Lee, Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.3
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    • pp.235-242
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    • 2011
  • In order to improve forage characteristics of Italian ryegrass by genetic transformation, an efficient callus induction from mature seed and optimal plant regeneration system were established using a domestic cultivar 'Kospeed'. Addition of 5 mg/L of 2,4-D showed highest frequency of embryogenic callus induction from mature seeds. N6 medium showed higher frequency of both callus induction and plant regeneration as compared with MS and SH medium. The highest plant regeneration frequency 67% was obtained when embryogenic calli were transferred to N6 medium containing 1 mg/L 2,4-D and 5 mg/L BA. Supplementation of regeneration medium with sucrose at 30 g/L level maximized regeneration frequency as compared to the other concentrations. These data would be very helpful for molecular breeding of domestic Italian ryegrass cultivar through genetic transformation.

Estimation of Nitrogen Optimum Level for Rice Planting after Italian Ryegrass (Lolium multiflorum Lam.) Cultivation (이탈리안 라이그라스 후작 벼 재배시 수량을 고려한 적정 질소시비량 추정)

  • Song, Yo-Sung;Park, Woo-Kyun;Lee, Ye-Jin;Lee, Jong-Sik;Yun, Hong-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.448-451
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    • 2011
  • To establish the optimum nitrogen level for rice planting after Italian ryegrass cultivation, one experiment was conducted on a normal paddy soil (Jeonbug series soil taxonomy) with six different nitrogen treatments for two years from 2009 to 2010. The treatments were including no nitrogen fertilization (Free N), 50%-Basal N, 75%-Basal N, 100%-Basal N, 150%-Basal N and 100% of basal fertilization with $30kg\;N\;ha^{-1}$ (100%+N30-Basal N) for decomposing of Italian ryegrass stubble. The highest rice yields were 8,420 obtained by 100%-Basal N. 150%-Basal N and 100%+N30-Basal N produced a rice yield of $8,190kg\;ha^{-1}$. Those of 50%-Basal N and Free N were produced 8,020 and $7,370kg\;ha^{-1}$, respectively. The correlation between rice yield and nitrogen treatment showed a quadratic relationship in high significant. According to this regression, the highest level of nitrogen treatment was $73kg\;ha^{-1}$ and the highest rice yield was $8,405kg\;ha^{-1}$. Nitrogen uptake rates were relatively higher with lower amounts of nitrogen fertilizer treated.

Effects of using different roughages in the total mixed ration inoculated with or without coculture of Lactobacillus acidophilus and Bacillus subtilis on in vitro rumen fermentation and microbial population

  • Miguel, Michelle;Mamuad, Lovelia;Ramos, Sonny;Ku, Min Jung;Jeong, Chang Dae;Kim, Seon Ho;Cho, Yong Il;Lee, Sang Suk
    • Animal Bioscience
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    • v.34 no.4
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    • pp.642-651
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    • 2021
  • Objective: This study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population. Methods: Three TMRs formulations composed of different forages were used and each TMR was grouped into two treatments: non-fermented TMR and fermented TMR (F-TMR) (inoculated with coculture of L. acidophilus and B. subtilis). After fermentation, the fermentation, chemical and microbial profile of the TMRs were determined. The treatments were used for in vitro rumen fermentation to determine total gas production, pH, ammonianitrogen (NH3-N), and volatile fatty acids (VFA). Microbial populations were determined by quantitative real-time polymerase chain reaction (PCR). All data were analyzed as a 3×2 factorial arrangement design using the MIXED procedure of Statistical Analysis Systems. Results: Changes in the fermentation (pH, lactate, acetate, propionate, and NH3-N) and chemical composition (moisture, crude protein, crude fiber, and ash) were observed. For in vitro rumen fermentation, lower rumen pH, higher acetate, propionate, and total VFA content were observed in the F-TMR group after 24 h incubation (p<0.05). F-TMR group had higher acetate concentration compared with the non-fermented group. Total VFA was highest (p<0.05) in F-TMR containing combined forage of domestic and imported source (F-CF) and F-TMR containing Italian ryegrass silage and corn silage (F-IRS-CS) than that of TMR diet containing oat, timothy, and alfalfa hay. The microbial population was not affected by the different TMR diets. Conclusion: The use of Italian ryegrass silage and corn silage, as well as the inoculation of coculture of L. acidophilus and B. subtilis, in the TMR caused changes in the pH, lactate and acetate concentrations, and chemical composition of experimental diets. In addition, F-TMR composed with Italian ryegrass silage and corn silage altered ruminal pH and VFA concentrations during in vitro rumen fermentation experiment.

A Study on Hay Preparation Technology for Italian Ryegrass Using Stationary Far-Infrared Dryer (정치식 원적외선 건조기를 이용한 이탈리안 라이그라스 건초 조제 기술 연구)

  • Jong Geun Kim;Young Sang Yu;Yan Fen Li;Li Li Wang;Hyun Rae Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.4
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    • pp.258-263
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    • 2022
  • This experiment was conducted to confirm the possibility of manufacturing artificial Italian ryegrass hay using far-infrared rays in Korea. The machine used in this experiment was a far-infrared ray dryer capable of adjusting temperature, airflow, and far-infrared radiation, and was conducted on Italian ryegrass harvested in May. Conditions for drying were performed by selecting a total of nine conditions, and each condition was set to emission rate of 42 to 45%, and the internal temperature was set to 65℃. The speed of the air flow in the machine was 40-60 m/s, and the overall drying time was 30 minutes for 42% radiation, 25 minutes for 43% radiation, and 20 minutes for 45% radiation. The final dry matter content according to each drying condition was 88.5% on average, and the dry matter content suitable for hay was shown in the all treatment. Looking at the power consumption according to the drying conditions, the lowest was found in the treatment that dried for 20 minutes at 45% radiation. In the drying rate, there was no difference in drying conditions 1 to 5, but a significantly low tendency was shown in conditions 6 to 7. In terms of feed value, CP and IVDMD were higher than raw materials in most drying conditions, and ADF and NDF contents were low, and tended to be high in drying conditions 4, 7, and 8. Through the above results, it was judged that drying conditions 7 and 8 were the most advantageous when considering drying speed, power consumption, and quality.

Yield Comparison Simulation between Seasonal Climatic Scenarios for Italian Ryegrass (Lolium Multiflorum Lam.) in Southern Coastal Regions of Korea (우리나라 남부해안지역에서 이탈리안 라이그라스에 대한 계절적 기후시나리오 간 수량비교 시뮬레이션)

  • Kim, Moonju;Sung, Kyung Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.1
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    • pp.1-9
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    • 2022
  • This study was carried out to compare the DMY (dry matter yield) of IRG (Italian ryegrass) in the southern coastal regions of Korea due to seasonal climate scenarios such as the Kaul-Changma (late monsoon) in autumn, extreme winter cold, and drought in the next spring. The IRG data (n = 203) were collected from various Reports for Collaborative Research Program to Develop New Cultivars of Summer Crops in Jeju, 203 Namwon, and Yeungam from the Rural Development Administration - (en DASH). In order to define the seasonal climate scenarios, climate variables including temperature, humidity, wind, sunshine were used by collected from the Korean Meteorological Administration. The discriminant analysis based on 5% significance level was performed to distinguish normal and abnormal climate scenarios. Furthermore, the DMY comparison was simulated based on the information of sample distribution of IRG. As a result, in the southern coastal regions, only the impact of next spring drought on DMY of IRG was critical. Although the severe winter cold was clearly classified from the normal, there was no difference in DMY. Thus, the DMY comparison was simulated only for the next spring drought. Under the yield comparison simulation, DMY (kg/ha) in the normal and drought was 14,743.83 and 12,707.97 respectively. It implies that the expected damage caused by the spring drought was about 2,000 kg/ha. Furthermore, the predicted DMY of spring drought was wider and slower than that of normal, indicating on high variability. This study is meaningful in confirming the predictive DMY damage and its possibility by spring drought for IRG via statistical simulation considering seasonal climate scenarios.

Effect of Monoculture and Mixtures on Dry Matter Yield and Feed Value of Italian Ryegrass (Lolium Multiflorum Lam.) (이탈리안 라이그라스의 단파 및 혼파 재배가 건물수량 및 사료가치에 미치는 영향)

  • Jeong Sung Jung;Bo Ram Choi;Ouk Kyu Han;Bae Hun Lee;Ki Choon Choi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.2
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    • pp.88-94
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    • 2023
  • This study was conducted to analyze and compare the dry matter yield of Italian ryegrass (IRG) cultivated under monoculture and mixed culture system to recommend suitable varieties that can be cultivated. Italian ryegrass cultivars, Green Fram (GF, extremely early-maturing), Kowinearly (KE, early-maturing), Kowinmaster (KM, mild-maturing), and Hwasan 104 (H104, late-maturing), were used for mono or mixed cultivation. The average monthly temperature in Cheonan over the past 30 years tended to be similar, but that in November and March are judged to be abnormal weather. The dry matter yield of GF+H104 was significantly higher during harvest than that of GF (p<0.05). The dry matter yields of KE and KE+KM were significantly higher during harvest than the output standards of KE and KM. There was no significant difference between the dry matter yield of H104 and KM (p>0.05), but KM had the highest yield of 16,763.1 kg/ha. Analysis showed that the highest dry matter yield during IRG harvest was obtained under monoculture and KE+KM mixed culture. Because the occurrence frequency of abnormal weather such as drought during spring is increasing recently, it is judged that IRG cultivation using early and middle growth is necessary to prepare for abnormal weather.

The effects of aqueous extracts of plant roots on germination of seeds and growth of seedings (식물근의 추출물질이 종자발아 및 유식물의 생장에 미치는 영향)

  • Chan-Ho Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.1-23
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    • 1968
  • This study aimed at contributing to the improvement of cropping systems after finding out the effects of excrements and components of crop root influence on other crops as well as themselves. The following forage crops suitable for our country were selected for the present study. Aqueous extracts of fresh roots, aqueous extracts of rotting roots and aqueous solutions of excrements of red clover, orchard grass and brome grass were studied for the effects influencing the germination and growth of seedlings of red clover, ladino clover, lespedeza, soybean, orchard grass, Italian ryegrass, brome grass, barley, wheat, sorghum, corn and Hog-millet. In view of the possibility that the organic acid might be closely related to the excrements and components of crop root connected with soil sickness, the acid components of three species of roots were analysed by paper chromatography and gas chromatography method. The following results were obtained: 1. Effects of Aqueous Extracts of Fresh Roots : Aqueous extracts of red clover: The extracts inhibited the growth of seedlings of the ladino clover and lespedeza and also inhibited the development of most crops except that of sorghum among the Graminaceae. Aqueous extracts of orchard grass: The extracts promoted the seedlings growth of red clover and soybean, while it inhibited the germination and growth of orchard grass. There were no noticeable effects influencing other crops while it inhibited the growth of barley and Hog-millet. Aqueous extracts of brome grass: There was no effect on Italian ryegrass but there was an inhibiting effect on the other crops. 2. Effects of Aqueous Extracts of Rotting Roots : Aqueous extracts of red clover: The extracts promoted the seedling growth of red clover. But it reflected the inhibiting effects on other crops except sorghum. Aqueous extracts of orchard grass: The extracts promoted the growth of red clover, ladino clover, soybean and sorghun, while it inhibited the germination and rooting of barley and Hog-millet. Aqueous extracts of brome grass: The extracts gave the promotive effects to the growth of red clover, soybean and sorghum, but caused inhibiting effects on orchard grass, brome grass, barley and Hog-millet. 3. Effects of Aqueous Solutions of Excrements : The aqueous solution of excrements of red clover reflected the inhibition effects to the growth of Graminaceae, while the aqueous solutions of excrements of orchard grass and Italian ryegrass caused the promotive effects on the growth of red clover. 4. Results of Organic Acid Analysis : The oxalic acid, citric acid, tartaric acid, malonic acid, malic acid and succinic acid were included in the roots of red clover as unvolatile organic acid, and in the orchard grass and brome grass there were included the oxalic acid, citric acid, tartaric acid and malic acid. And formic acid was confirmed in the red clover, orchard grass and brome grass as volatile organic acid. In consideration of the results mentioned in above the effects of excrements and components of roots found in this studies may be summarized as follows. 1) The red clover generally gave a disadvantageous effect on the Graminaceae. Such trend was considered chiefly caused by the presence of many organic acids, namely oxalic, citric, tartaric, malonic, malic, succinic and formic acid. 2) The orchard grass generally gave an advantageous effect on the Leguminosae. This may be due to a few kinds of organic acid contained in the root, namely oxalic, citric, tartaric, malic and formic acid. Furthermore a certain of promotive materials for growth was noted. 3) As long as the root of brome grass are not rotten, it gave a disadvantageous effect on the Leguminosae and Graminaceae. This may be due to the fact that several unidentified volatile organic acid were also included besides the confirmed organic acid, namely oxalic, citric, tartaric, malic and formic acid. 5. Effects of Components in Roots to the Soil Sickness : 1) It was considered that the cause of alleged red clover's soil sickness did not result from the toxic components of the roots. 2) It was recognized that the toxic components of roots might be the cause of soil sickness in case the orchard grass and brome grass were put into the long-term single cropping. 6. Effects of Rooted Components to the Companion Crops in the Cropping System : a) In case of aqueous extracts of fresh roots and aqueous excrements (Inter cropping and mixed cropping) : 1) Advantageous combinations : Orchard grass->Red clover, Soybean, Italian ryegrass->Red clover, 2) Disadvantageous combinations : Red clover->Ladino clover, Lespedeza, Orchard grass, Italian ryegrass, Fescue Ky-31, Brome grass, Barley, Wheat, Corn and Hog.millet, Orchard grass->Lespedeza, Orchard grass, Barley and Hog-millet, Brome grass->Red clover, Ladino clover, Lespedeza, Soybean, Orchard grass, Brome grass, Barley, Wheat, Sorghum, Corn and Hog-millet, 3) Harmless combinations : Red clover->Red clover, Soybean and Sorghum, Orchard grass->Ladino clover, Italian ryegrass, Brome grass, Wheat, Sorghum and Corn, Brome grass->Italian ryegrass, b) In case of aquecus extracts of rotting roots(After cropping) : 1) Advantageous combinations : Red clover->Red clover and Sorghum, Orchard grass->Red clover, Ladino clover, Soybean, Sorghum, and Corn, Brome grass->Red clover, Soybean and Sorghum, 2) Disadvantageous combinations : Red clover->Lespedeza, Orchard grass, Italian ryegrass, Brome grass, Barley, Wheat, and Hog-millet Orchard grass->Barley and Hog-millet, Brome grass->Orchard grass, Brome grass, Barley and Hog-millet, 3) Harmless combinations : Red clover->Ladino clover, Soybean and Corn, Orchard grass->Lespedeza, Orchard grass, Italian ryegrass, Brome grass and Wheat Brome gass->Ladino clover, Lespedeza, Italian ryegrass and Wheat.

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