• 통합자연과학논문집
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• 제8권2호
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• pp.117-127
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• 2015

Lentil (Lens culinaris Medik.) is one of the important legumes and cheaper source of protein in Bangladesh that displays great biological diversity. Isozyme, one of the most important protein markers to detect genetic polymorphism in lentil, whereas we considered thirteen-isozyme in six varieties viz., BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. The highest polymorphism was found in tyrosinase isozyme system. UPGMA analysis revealed that the highest similarity between BARI masur-5 and BARI masur-6 whereas, the highest genetic distance between BARI masur-1 and BARI masur-5 reflecting higher intervarietal variation. Principal component analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 81.58%, 11.19% and 4.94% variation respectively, with cumulative variation of the first three PCs was 75.45%. Consequently, Isozyme could clearly assed the genetic diversity at intervarietal levels and these two varieties can be considered as valuable gene resources for future breeding and conservation programs.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1988년도 춘계 학술대회지
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• pp.12-13
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• 1988

• 한국균학회지
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• 제25권3호통권82호
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• pp.176-190
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• 1997

Isozyme PAGE에 의한 isozyme polymorphism 및 random amplified polymorphic DNA(RAPD) pattern의 비교를 통하여 한국에서 수집된 63개의 표고 버섯(Lentinus edodes)품종의 유연관계를 조사하여 보았다. NTSYS PC program을 통하여 비교에 사용되어진 3가지 isozyme 즉 esterase, peroxidase, acid phophatase 등은 각각 10, 7, 3개의 isozyme polymorphism type으로 나뉘어질 수 있었고, 이러한 3개의 isozyme type들은 group-average method를 이용하여 최종적으로 6개의 집단으로 구분되어질 수 있었다. 또한 RAPD를 통한 표고 품종간 유연관계 비교는 사용되어진 총 20개의 random primer 들중 3개의 primer(OPA 03, OPA 18, OPA 19)가 품종간 polymorphism을 보였고 사용되어진 전체 random primer의 PCR product들의 집단 분석을 통해 최종적으로 5개의 집단으로 구분할 수 있었다. 사용되어진 두 가지 방법 즉, isozyme polymorphism및 RAPD pattern에 의해 구분되어진 63개 표고 버섯 품종의 subgroup level에서의 절대적인 유사성은 관찰할 수 없었다. Yii-Mattila(1996) 등에 의하면 품종간 비교시 isozyme analyses에서와는 달리 RAPD analyses에서는 많은 수의 strain-specific band들이 얻어질 수 있고 이것이 두 방법간의 유사성을 관찰하지 못하게 하는 원인이라고 보고하였다.

• 한국잠사곤충학회지
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• 제45권1호
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• pp.18-24
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• 2003

한반도에 서식하는 멧누에나방의 서식지역에 따른 유전적 변이와 집누에나방과의 종간 유연관계를 조사하였다. 멧누에나방의 isozyme 및 체액단백질을 대상으로 유전자 빈도를 조사한 결과, 조사지역 중 경북 칠곡과 경남 진주집단 사이의 유전적 유사도가 가장 높았으며, 경남 진주와 강원 고성집단 사이에서 가장 낮게 나타났다. 그러나 서식집단 상호간의 유사도는 매우 높았다. 한국산 멧누에나방과 집누에나방의 isozyme 유전자형은 대부분이 동일하게 발현되었으나, 집누에나방의 한국종에서만 발현된다고 알려진 Bes의 B형, Amy-hc의 F형, Ict-E의 n형 및 Ict-h의 M형과 S형 등의 유전자형이 한반도에 서식하는 멧누에나방에서 발현되었다. 따라서, 한반도 내에서 멧누에나방의 서식지역간 유전적 변이는 인정되지 않으며, 지금까지는 고려되지 않았던 집누에 한국종과 한국산 멧누에나방의 종간 유연관계에 대해 새로운 의문이 야기된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권1호
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• pp.1-7
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• 2007

In order to find out the appropriate parents for the breeding programme, twelve bivoltine and three multivoltine silkworm breeds were evaluated on the basis of multivariate selection index and isozyme analysis. Of which, four [CSR2, D6 (P), SK3, SK4] bivoltine and two multivoltine (Nistari, Cambodge) breeds were selected and breeding initiated to develop higher survival bivoltine silkworm breed suitable for tropical conditions. Among two isozyme (Esterase and acid phosphatase) analyzed, only esterase exhibited polymorphism among the bivoltine breeds. No polymorphism was observed among multivoltine in respect of esterase as well as acid phosphatase.

• 한국패류학회지
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• 제12권1호
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• pp.39-45
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• 1996

Polyacrylamide gel electrophoresis was used to reveal the esterase isozyme patterns of a freshwater snail, Semisulcospira gottschei, in the lake Uiam. From the result with inhibitors, it is tentatively concluded that four types (arylesterase, carboxylesterase, acethylesterase, acethylcholinesterase)of the adult(means of allele per locus=1.16, %polymorphism=16.7, heterozygosity=0.082)was lower than the juveniles(A=1.7, %P=16.7, H$\sub$D/=0.2215).

• 한국잠사곤충학회지
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• 제39권2호
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• pp.119-133
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• 1997

Isozyme was used to characterize general protein patterns of genetic relationships among 303 silkworm stocks preserved in National Sericultural and Entomology Research Institute, RDA. Six isozymes (esterase, acid phosphatase, alkaline phosphatase, amylase, glucose-6-phosphate dehydrogenase and sucrase) from hemolymph, midgut, and digestive juice were employed to construct dendograms(UPGMA method) using a polycrylamide gel electrophoresis. A cluster analysis revealed four major group, which were divided into several subgroups within each group, contained assemglages of Japanese and Chinese races. Especially, genetic differentiation in the first and second group was greatest rather than within Japanese and Chinese races repectively and was concordant with the hypothesis of phyletic sorting of initial variability in China many years ago. Hypothesized recent introgression between groups was also plausible, but the eviednce suggested bidirectional gene flow between the Chinese and the Japnaese lineages. Interpreting the results in light of evidence from the current study, the genetic diversity and relationship showed in Korean silkworm race, Hansammyun reflected early and independent evolution from the Chinese ancestor, limited addition of new variability and phyletic sorting within Korean peninsula more than 4,000 years.

• Journal of Plant Biotechnology
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• 제7권2호
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• pp.105-112
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• 2005

Buffer soluble protein and five isozymes were analyzed to assess the inter specific relationship among 25 species of the genus Mammillaria Haw. A total of 102 types of proteins were resolved, out of which eighty-six types were found to be polymorphic and only two were unique. A total of 248 bands (isoforms) were detected for 5 isozymes, among them only 4 were found to be monomorphic and 35 were exclusive. Mantel 'Z' statistics revealed wide variations in the correlation among different enzymes. The correlation value 'r' was the highest in case of esterase with pooled data of all the five enzymes. The dendrogram constructed on the basis of pooled data (protein and allozyme) divided the species into two major clusters containing 14 and 11 members respectively. The species M. matudae and M. bella were found to be the most closely related while M. decipience and M. camptroticha were distantly apart. The present study gave an indication of usefulness of the isozyme and protein markers for genetic discrimination between different species of Mammillaria.

• 한국응용곤충학회지
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• 제37권1호
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• pp.19-22
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• 1998

파밤나방(Spodoptera exigua(H bner))의 유전지표를 결정하기 위해 17종 동위효소의 좌위수, 대립유전자빈도 및 각 효소의 4차구조가 분석되었다. 총 분석된 좌우수는 30개였으며, 이중 70.0%가 다형유전좌위를 보였다. 좌위당 유효대립유전자수는 1.72개였고 평균이형접합율(${H}_{e}$)은 32.8%로 추정되었다. 조사된 집단의 동계교배효과는 (F)는 21.0%이었다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.232-233
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• 2001

The isozymic polymorphism among the Oncorhynchus masou subspecies (O. m. masou and O. m. ishikawai) and the genetic variation in the O. mykiss species (rainbow trout and its albino mutant) were examined in present study. The biochemical marker might be identified at the sublevel of species. The aims of this study is to examine such feasibility of using the isozyme polymorphisms of the fish management and characterization between subspecies or morphomutants such as albinism. (omitted)