• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.147-149
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• 1999

Biotransformation of exogenous (-)-(4R)-isopiperitenone in cell suspension cultures of Mentha piperita resulted in oxidized products, with (-)-7-hydroxyisopiperitenone being the major compound. The mass of products obtained $unde^{18}O_2$, atmosphere was two units higher than that under normal atmosphere. The biotransformation was inhibited by several cytochrome P450-specific inhibitors as well as by carbon monoxide. Carbon monooxide inhibition was substantially overcome by irradiation of cells with blue light including light at 450nm wavelength. These results suggested that a cytochrome P450-type monooxygenase was involved in the biotransformation.

• Applied Biological Chemistry
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• 제36권5호
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• pp.358-363
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• 1993

박하(Mentha piperita) 세포현탁배양에(-)-menthol 생합성 중간체를 투여하여 배양된 세포의 대사경로를 연구하였다. (-)-Limonene을 투여 하였을 때 이는 다른 대사물로 변환되지 않는 것으로 관찰 되었다. (+)-Pulegone은 (+)-isomenthone 및 (-)-menthone으로 변환되었으며, (-)-menthone은 (-)-menthol로 변환되었다. 이 실험은 현탁배양세포가 대부분의 생합성 활성을 유지하고 있으며 (-)-limonene hydroxylase의 활성이 제한적임을 보여 주었다. (-)-Isopiperitenone을 투여하였을 때는 (+)-pulegone, piperitenone, (-)-7-hydroxyisopiperitenone, (R)- 및 (S)-6-hydroxyisopiperitenone이 생성되었다.