Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Menthol biosynthesis pathway in Mentha piperita suspension cells

박하(Mentha piperita) 세포 현탁배양에서 멘톨생합성 경로

  • Park, Si-Hyung (The Research Center for New Bio-Materials in Agriculture and Department of Agricultural Chemistry, Seoul National University) ;
  • Chae, Young-Am (The Research Center for New Bio-Materials in Agriculture and Department of Department of Agronomy, Seoul National University) ;
  • Lee, Hyong-Joo (The Research Center for New Bio-Materials in Agriculture and Department of and Department of Food Sciences and Technology, Seoul National University) ;
  • Kim, Soo-Un (The Research Center for New Bio-Materials in Agriculture and Department of Agricultural Chemistry, Seoul National University)
  • 박시형 (서울대학교 농업 생물신소재연구센터 및 농화학과) ;
  • 채영암 (서울대학교 농업 생물신소재연구센터 및 농학과) ;
  • 이형주 (서울대학교 농업 생물신소재연구센터 및 식품공학과) ;
  • 김수언 (서울대학교 농업 생물신소재연구센터 및 농화학과)
  • Published : 1993.10.31
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Abstract

The metabolic capability of the cultured cells of peppermint was tested with whole intact cells by feeding appropriate exogenous substrates to the suspension cultures. Conversion of (-)-limonene into any other monoterpenes was not observed with the suspension cultures. (+)-Pulegone was converted into (+)-isomenthone and (-)-menthone, and (-)-menthone into (-)-menthol. The experiments confirmed that the suspension retained most of the menthol biosynthesis pathway in the cell except for a few loci. (-)-Isopiperitenone was transformed into (+)-pulegone, piperitenone, (-)-7-hydroxyisopiperitenone and two unidentified products.

박하(Mentha piperita) 세포현탁배양에(-)-menthol 생합성 중간체를 투여하여 배양된 세포의 대사경로를 연구하였다. (-)-Limonene을 투여 하였을 때 이는 다른 대사물로 변환되지 않는 것으로 관찰 되었다. (+)-Pulegone은 (+)-isomenthone 및 (-)-menthone으로 변환되었으며, (-)-menthone은 (-)-menthol로 변환되었다. 이 실험은 현탁배양세포가 대부분의 생합성 활성을 유지하고 있으며 (-)-limonene hydroxylase의 활성이 제한적임을 보여 주었다. (-)-Isopiperitenone을 투여하였을 때는 (+)-pulegone, piperitenone, (-)-7-hydroxyisopiperitenone, (R)- 및 (S)-6-hydroxyisopiperitenone이 생성되었다.

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