• Title/Summary/Keyword: isolation systems

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Minimum Number of Input Ground-motions to Assess Seismic Performance of Nuclear Facilities (원전시설의 내진성능평가를 위한 입력지반운동의 최소개수)

  • Hong, Kee-Jeung;Choi, Ji-Hae;Kim, Hyun-Uk;Joo, Kwang-Ho
    • Journal of the Earthquake Engineering Society of Korea
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    • v.20 no.5
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    • pp.341-349
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    • 2016
  • Currently, researches are being actively conducted in assessing seismic performance of nuclear facilities in USA and Europe. In particular, applying this technique of assessing seismic performance to design of isolation systems in nuclear power plants is being performed and then ASCE 4 Draft (2013) is being revised accordingly in the United States. In order to satisfy the probabilistic performance objectives described by seismic responses with certain confidence levels (ASCE 43, 2005), the probability distributions of these responses have to be defined. What is the minimum number of input ground-motions to obtain the probability distribution precise enough to represent the unknown actual distribution? Theoretical basis, for how to determine the minimum number of input ground-motions for given a logarithmic standard deviation to approximate the unknown actual median of the log-normal distribution within a range of error at a certain level of confidence, is introduced by Huang et al. (2008). However, the relationship between the level of confidence and the range of error is not stated in the previous study. In this paper, based on careful reviews on the previous work, the relationship between the level of confidence and the range of error is logically and explicitly stated. Furthermore, this relationship is also applied to derive the minimum number of input ground-motions in order to approximate the unknown actual logarithmic standard deviation. Several recommendations are made for determining the minimum number of input ground-motions in probabilistic assessment on seismic performance of facilities in nuclear power plants.

Estimation of Effect Zone for the Establishment of Damage-Minimizing Plan of Chemical Plants (화학공장의 사고피해 최소화 대책수립을 위한 영향범위 평가)

  • Lee, Hern-Chang;Han, Seong-Hwan;Cho, Ji-Hoon;Shin, Dong-Il;Kim, Tae-Ok
    • Journal of the Korean Institute of Gas
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    • v.15 no.2
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    • pp.69-74
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    • 2011
  • As a way to propose a method for the establishment of practical damage-minimizing plan for chemical plants, the release scenario was established by using API-581 BRD and the effect zone was estimated by the KS-RBI program supporting the quantitative cause analysis. And the risk assessment was performed. As a result, we found that to minimize the damage of a chemical plant, it is effective to use four release hole diameters (small, medium, large and rupture) and release time estimated according to the classes of detection and isolation systems. In addition, it can be safely said that through appling the damage areas considering weighted average by failure frequency and worst-case simultaneously, industrial sites can come up with the effective emergency response plan.

Isolation and Characterization of a Theta Glutathione S-transferase Gene from Panax ginseng Meyer

  • Kim, Yu-Jin;Lee, Ok-Ran;Lee, Sung-Young;Kim, Kyung-Tack;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.36 no.4
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    • pp.449-460
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    • 2012
  • Plants have versatile detoxification systems to encounter the phytotoxicity of the wide range of natural and synthetic compounds present in the environment. Glutathione S-transferase (GST) is an enzyme that detoxifies natural and exogenous toxic compounds by conjugation with glutathione (GSH). Recently, several roles of GST giving stress tolerance in plants have demonstrated, but little is known about the role of ginseng GSTs. Therefore, this work aimed to provide further information on the GST gene present in Panax ginseng genome as well as its expression and function. A GST cDNA (PgGST) was isolated from P. ginseng cDNA library, and it showed the amino acid sequence similarity with theta type of GSTs. PgGST in ginseng plant was induced by exposure to metals, plant hormone, heavy metals, and high light irradiance. To improve the resistance against environmental stresses, full-length cDNA of PgGST was introduced into Nicotiana tabacum. Overexpression of PgGST led to twofold increase in GST-specific activity compared to the non-transgenic plants, and the GST overexpressed plant showed resistance against herbicide phosphinothricin. The results suggested that the PgGST isolated from ginseng might have a role in the protection mechanism against toxic materials such as heavy metals and herbicides.

Green Fluorescent Protein-reporter Mammalian One-hybrid System for Identifying Novel Transcriptional Modulators for Human $p14^{ARF}$ Tumor Suppressor Gene

  • Lee, Hye Jin;Yang, Dong Hwa;Yim, Tae Hee;Rhee, Byung Kirl;Kim, Jung-Wook;Lee, Jungwoon;Gim, Jin Bae;Kim, JungHo
    • Animal cells and systems
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    • v.6 no.4
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    • pp.317-322
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    • 2002
  • To improve conventional yeast one-hybrid screening, we have developed an efficient mammalian one-hybrid system that allows rapid isolation of com-plementary DNAs which are able to induce human p14$^{ARF}$. tumor suppressor gene. A 1.5 kb promoter region of p14$^{ARF}$ was fused to EGFP to generate ARF promoter-EGFP reporter vector. This reporter plasmid was stably trans-fected into NIH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F-1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence using FACS. The GFP-positive cells were cloned directly from the cultured cells and expanded in bulk culture. The genomic DNAs from GFP-positive cells were prepared and the CDNA insert in integrated retroviral genome was recovered by PCR using primers annealing to the retroviral vector sequences flanking the insert-cloning site. This system should be useful for efficient screening of expression CDNA libraries in mammalian cells to identify novel upstream regulators for spe-cific genes by one-hybrid interaction.ion.

Evaluation of The Nonlinear Seismic Behavior of a Biaxial Hollow Slab (2방향 중공슬래브 구조시스템의 비선형 지진거동 평가)

  • Park, Yong-Koo;Kim, Hyun-Su;Ko, Hyun;Park, Hyun-Jae;Lee, Dong-Guen
    • Journal of the Earthquake Engineering Society of Korea
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    • v.15 no.1
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    • pp.1-10
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    • 2011
  • Recently, there has been an increased interest in the noise isolation capacity of floor slabs, and thus an increase of slab thickness is required. In addition, long span floor systems are frequently used for efficient space use of building structures. In order to satisfy these requirements, a biaxial hollow slab system has been developed. To verify the structural capacity of a biaxial hollow slab system, safety verification against earthquake loads is essential. Therefore, the seismic behavior of a biaxial hollow slab system has been investigated using material nonlinear time history analyses. For efficient time history analyses, the equivalent plate element model previously proposed was used and the seismic capacity of the example structure having a biaxial hollow slab system has been evaluated using the nonlinear finite element model developed by the equivalent frame method. Based on analytical results, it has been shown that the seismic capacity of a biaxial hollow slab system is not worse than that of a flat plate slab system with the same thickness.

Isolation and inheritance of microsatellite loci for the oily bittering (Acheilognathus koreensis): applications for analysis of genetic diversity of wild populations

  • Kim, Woo-Jin;Kong, Hee-Jeong;Shin, Eun-Ha;Kim, Chi-Hong;Kim, Hyung-Soo;Kim, Young-Ok;Nam, Bo-Hye;Kim, Bong-Seok;Lee, Sang-Jun;Jung, Hyung-Taek
    • Animal cells and systems
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    • v.16 no.4
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    • pp.321-328
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    • 2012
  • The oily bittering Acheilognathus koreensis is a freshwater species that is endemic to Korea and is experiencing severe declines in natural populations as a result of habitat fragmentation and water pollution. For the conservation and restoration of this species, it is necessary to assess its genetic diversity at the population level. We developed 13 polymorphic microsatellite loci that were used to analyze the genetic diversity of two populations collected from the Kum River and the Tamjin River in Korea. All loci exhibited Mendelian inheritance patterns when examined in controlled crosses. Both populations revealed high levels of variability, with the number of alleles ranging from 3 to 20 and observed and expected heterozygosities ranging from 0.500 to 0.969 and from 0.529 to 0.938, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium, and one pair of loci showed significant linkage disequilibrium after Bonferroni correction. Pairwise $F_{ST}$ and genetic distance estimation showed significant differences between two populations. These results suggest that the microsatellites developed herein can be used to study the genetic diversity, population structure and conservation measure of A. koreensis.

Isolation and characterization of micro satellite loci in the Korean crayfish, Cambaroides similis and application to natural population analysis

  • Ahn, Dong-Ha;Park, Mi-Hyun;Jung, Jae-Ho;Oh, Mi-Jin;Kim, Sang-Hee;Jung, Jong-Woo;Min, Gi-Sik
    • Animal cells and systems
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    • v.15 no.1
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    • pp.37-43
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    • 2011
  • The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight micro satellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These micro satellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

Design of a Compact Antenna Array for Satellite Navigation System Using Hybrid Matching Network

  • Lee, Juneseok;Cho, Jeahoon;Ha, Sang-Gyu;Choo, Hosung;Jung, Kyung-Young
    • Journal of Electrical Engineering and Technology
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    • v.13 no.5
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    • pp.2045-2049
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    • 2018
  • An antenna arrays for a satellite navigation systems require more antenna elements to mitigate multiple jamming signals. In order to maintain the small array size while increasing the number of antenna elements, miniaturization technique is essential for antenna design. In this work, an electrically small circular microstrip patch antenna with a 3 dB hybrid coupler is designed as an element antenna, where the 3 dB hybrid coupler can yield the circularly polarized radiation characteristic. The miniaturized element antenna typically has too large capacitance in GPS L1 and GLONASS G1 bands, making it difficult to match with a single stand-alone non-Foster matching circuit (NFMC) in a stable state. Therefore, we propose a new matching technique, referred to as the hybrid matching method, which consists of a NFMC and a passive circuit. This passive tuning circuit manages reactance of antenna elements at an appropriate capacitance without a pole in the operating frequency range. The antenna array is fabricated, and the measured results show a reflection coefficient of less than -10 dB and an isolation of greater than 50 dB. In addition, peak gain of the proposed antenna is increased by 22.3 dB compared to the antenna without the hybrid matching network.

GPGPU Task Management Technique to Mitigate Performance Degradation of Virtual Machines due to GPU Operation in Cloud Environments (클라우드 환경에서 GPU 연산으로 인한 가상머신의 성능 저하를 완화하는 GPGPU 작업 관리 기법)

  • Kang, Jihun;Gil, Joon-Min
    • KIPS Transactions on Computer and Communication Systems
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    • v.9 no.9
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    • pp.189-196
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    • 2020
  • Recently, GPU cloud computing technology applying GPU(Graphics Processing Unit) devices to virtual machines is widely used in the cloud environment. In a cloud environment, GPU devices assigned to virtual machines can perform operations faster than CPUs through massively parallel processing, which can provide many benefits when operating high-performance computing services in a variety of fields in a cloud environment. In a cloud environment, a GPU device can help improve the performance of a virtual machine, but the virtual machine scheduler, which is based on the CPU usage time of a virtual machine, does not take into account GPU device usage time, affecting the performance of other virtual machines. In this paper, we test and analyze the performance degradation of other virtual machines due to the virtual machine that performs GPGPU(General-Purpose computing on Graphics Processing Units) task in the direct path based GPU virtualization environment, which is often used when assigning GPUs to virtual machines in cloud environments. Then to solve this problem, we propose a GPGPU task management method for a virtual machine.

Isolation Control High Speed Transfer Switch for Upgrade Reliability of Uninterruptible Power Supply (USP의 신뢰성 향상을 위한 독립제어 고속절환장치)

  • Jung, Hyun-Chul;Hyun, Dong-Seok
    • The Transactions of the Korean Institute of Power Electronics
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    • v.13 no.4
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    • pp.278-286
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    • 2008
  • This paper investigates the fault generation type and the cause of output interruptionsin bulky with $30^{\sim}500[KVA]$ double conversion UPS, and proposes the fault detection method to improve the reliability of power supply used in the critical load in industry. Identifies its existing way of detecting a quality of inverter output it to bypass when exceeds its expectation. Under a UPS managing system, when an inner (Power device, Controller, CPU) fault occurs it disrupts the power supply and these occurrences has been verified by the results of experiments and application results. To overcome these problems, the proposed method constructs independently a fault-detection, a bypass-control device and a triple power supply apart from the conventional UPS operation. Also the detection point is changed to the preceding of a circuit breaker, a reference of fault detection is modified to avoid any clash and the breaking equipment is attached to intercept a spread of accident. As a result of applications of these developed systems to 242 UPS which was installed purposefully to the communication power supply, the service errors has not occurred in the UPS for two years since 2006.