• Molecular & Cellular Toxicology
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• v.2 no.1
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• Asian Journal of Turfgrass Science
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• v.4 no.1
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• pp.12-23
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• 1990

The chlorophyll-protein complexes were separated from thylakoid membranes of Spinach oleracea and Zoysia japonica by two gel Systems of LiDodSO4-PAGE and LiDodSo4/Urea- PAGE under nondenaturing conditions. Seven chlorophyll~protein complexes of CPI*, CPI, CPII*. CP47, CP43, CP29 and CPII were fractionated from both S,oleracea and Zjaponica by LiDodSO4-PAGE. CPI, CP47 and CP43 contained more chlorophyll a than chlorophyll b. The patterns of their absorption spectra at room temperature were similliar to that of chlorophyll a, judging by their UV-spedtroscopy. On the other hand, CPII* and CPII contained approximately equim-olar quantities of chlorophyll a and b. Additional five chlorophyll-protein complexes not separated in the LiDodSO4-PAGE system were electrophoretically isolated from both S, oleracea and Zjaponica by LiDodSO4/Urca-PAGE. The chlorophyll-protein complex just above LRCII $\alpha$in the gel appears CCII-RC separeted recently. 23 kDa and 20 kDa cho-protein complexes is probably LHCIa and LHCIb as judged from their molecular weight. Two novel chlorophyll~protein complexes designated "CPI7" and "CPI6" were fractionate by this gel system. Their molecular weights respectively. Although the stoichiometry of their components and their roles in thylakoid membranes are not apparant, It is thought that they are another kinds of LHCI.other kinds of LHCI.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.20 no.10
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• pp.102-111
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• 2021

Maintainability is a major design parameter that includes availability as well as reliability in a RAM (reliability, availability, maintainability) analysis, and is an index that must be considered when developing a system. There is a lack of awareness of the importance of predicting and analyzing maintainability; therefore, it is dependent on past-experience data. To improve the utilization rate, maintainability must be managed as a key indicator to meet the user's requirements for failure maintenance time and to reduce life-cycle costs. To improve the maintainability-prediction accuracy in the detailed design stage, we present a maintainability-prediction method that applies Method B of the Military Standardization Handbook (MIL-HDBK-472) Procedure V, as well as a Korean maintainability-prediction software package that reflects the system complexity.

• Journal of IKEEE
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• v.23 no.2
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• pp.474-478
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• 2019

In this paper, we analyze the degradation of receiving sensitivity due to the coupling between digital noise and mobile handset antenna using characteristic mode. First, we analyze the coupling mechanism between the antenna and digital noise, and analyze the role of the decoupling capacitor of the ground signal line, which is one of the ways to improve the antenna receiving sensitivity degradation due to camera noise. For the analysis, the digital signal line and the ground line of the FPCB of the camera module are modeled as a loop type feeder that excites the characteristic mode of the PCB ground, and improved model which has a ground line with a capacitor are analyzed.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.11
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• pp.5588-5613
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• 2018

Virtualization technology has been widely applied in the area of computer security research that provides a new method for system protection. It has been a hotspot in system security research at present. Virtualization technology brings new risk as well as progress to computer operating system (OS). A multi-level perception security model using virtualization is proposed to deal with the problems of over-simplification of risk models, unreliable assumption of secure virtual machine monitor (VMM) and insufficient integration with virtualization technology in security design. Adopting the enhanced isolation mechanism of address space, the security perception units can be protected from risk environment. Based on parallel perceiving by the secure domain possessing with the same privilege level as VMM, a mechanism is established to ensure the security of VMM. In addition, a special pathway is set up to strengthen the ability of information interaction in the light of making reverse use of the method of covert channel. The evaluation results show that the proposed model is able to obtain the valuable risk information of system while ensuring the integrity of security perception units, and it can effectively identify the abnormal state of target system without significantly increasing the extra overhead.

• Nuclear Engineering and Technology
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• v.53 no.3
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• pp.967-973
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• 2021

In 2011, an earthquake and subsequent tsunami hit the Fukushima Daiichi Nuclear Power Plant, causing simultaneous accidents in several reactors. This accident shows us that if there are several reactors on site, the seismic risk to multiple units is important to consider, in addition to that to single units in isolation. When a seismic event occurs, a seismic-failure correlation exists between the nuclear power plant's structures, systems, and components (SSCs) due to their seismic-response and seismic-capacity correlations. Therefore, it is necessary to evaluate the multi-unit seismic risk by considering the SSCs' seismic-failure-correlation effect. In this study, a methodology is proposed to obtain the seismic-response-correlation coefficient between SSCs to calculate the risk to multi-unit facilities. This coefficient is calculated from a probabilistic multi-unit seismic-response analysis. The seismic-response and seismic-failure-correlation coefficients of the emergency diesel generators installed within the units are successfully derived via the proposed method. In addition, the distribution of the seismic-response-correlation coefficient was observed as a function of the distance between SSCs of various dynamic characteristics. It is demonstrated that the proposed methodology can reasonably derive the seismic-response-correlation coefficient between SSCs, which is the input data for multi-unit seismic probabilistic safety assessment.

• Journal of Species Research
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• v.10 no.1
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• pp.12-22
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• 2021

During a comprehensive investigation of indigenous prokaryotic species in Korea, 25 and 10 bacterial strains assigned to the classes Alphaproteobacteria and Betaproteobacteria, respectively, were isolated from diverse environmental habitats, including soil, mud, tidal field, sea water, sand, rusted iron, and leaf. Based on their high 16S rRNA gene sequence similarities (>98.7%) and the formation of robust phylogenetic clades with type species, each strain was assigned to an independent and predefined bacterial species. Since there were no published or official reports regarding these 35 isolates in Korea, they - 25 species of 14 families in the 5 orders of Alphaproteobacteria and 10 species of 3 families in the two orders of Betaproteobacteria - have been reported as unrecorded species in Korea. In addition, Gram reaction, colony and cell morphology, basic biochemical characteristic, isolation source, and strain ID of each species are also described in the species description sections.

• Journal of Species Research
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• v.12 no.3
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• pp.212-223
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• 2023

A total of 30 bacterial strains were identified in the classes Betaproteobacteria and Gammaproteobacteria in the study of prokaryotic species in Korea. These strains were isolated from a variety of environmental sources, including soil, tidal flat, mud, wetland, pine cone, seaweed, sea sediment, and brackish water. Phylogenetic analysis showed that isolates were identified based on high 16S rRNA gene sequence similarities (≥98.7%) with the predefined bacterial type species. In this study, we present data on previously unreported species from Korea, including 10 species from three families of one order in the class Betaproteobacteria and 20 species from 12 families of nine order in the class Gammaproteobacteria. Morphological, biochemical characteristics, isolation sources, and NIBR deposit numbers are provided in the description sections.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2003.09a
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• pp.261-278
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• 2003

Concepts.Wafer-Level Chip-Scale Concept with Handling Substrate.Low Accuracy Placement Layout with Isolation Trench.Possible Pitch of Interconnections down to $10{\mu}{\textrm}{m}$ (Sn-Grains).Wafer-to-Wafer Equipment Adjustment Accuracy meets this Request of Alignment Accuracy (+/-1.5 ${\mu}{\textrm}{m}$).Adjustment Accuracy of High-Speed Chip-to-Wafer Placement Equipment starts to meet this request.Face-to-Face Modular / SLID with Flipped Device Orientation.interchip Via / SLID with Non-Flipped Orientation SLID Technology Features.Demonstration with Copper / Tin-Alloy (SLID) and W-InterChip Vias (ICV).Combination of reliable processes for advanced concept - Filling of vias with W as standard wafer process sequence.No plug filling on stack level necessary.Simultanious formation of electrical and mechanical connection.No need for underfiller: large area contacts replace underfiller.Cu / Sn SLID layers $\leq$ $10{\mu}{\textrm}{m}$ in total are possible Electrical Results.Measurements of Three Layer Stacks on Daisy Chains with 240 Elements.2.5 Ohms per Chain Element.Contribution of Soldering Metal only in the Range of Milliohms.Soldering Contact Resistance ($0.43\Omega$) dominated by Contact Resistance of Barrier and Seed Layer.Tungsten Pin Contribution in the Range of 1 Ohm

• The Plant Pathology Journal
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• v.39 no.2
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• pp.181-190
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• 2023

The fungal pathogen Pseudocercospora fuligena, known to affect tomatoes in the tropics and subtropics, has been reported from temperate climates including the United States and Turkey in recent years. In this study, an isolate from fresh tomatoes and the disease it causes were characterized and infection mechanisms investigated. Macroscopically, both sides of tomato leaves show indistinct effuse patches but prolific production of fuliginous lesions is conspicuous on the abaxial side first but also on the adaxial side later on as infection progressed. Microscopically, fascicles of conidiophores (11-128 ㎛ × 3.5-9 ㎛) arising from stromata and conidia with up to 12 septations were observed. Molecular characterization of the isolate revealed high homology (99.8%) to other P. fuligena isolated from tomatoes in Turkey. Out of the 10 media tested, P. fuligena grew significantly well and sporulated better on unsealed tomato oatmeal agar and carrot leaf decoction agar, both supplemented with CaCO₃. Direct transfer of conidia from profusely sporulating lesions was the easiest and quickest method of isolation for in-vitro studies. Light and scanning electron microscopy on cleared and intact tomato leaves further confirmed stomatal penetration and egress as well as prevalence of primary and secondary infection hyphae. In situ, blocked stomatal aperture areas of 154, 401, and 2,043 ㎛² were recorded at 7, 12, and 17 days after inoculation, respectively. With the recent expanded horizon of the pathosystem and its consequential impact, such studies will be useful for a proper diagnosis, identification and management of the disease on tomato worldwide.