• Restorative Dentistry and Endodontics
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• v.42 no.1
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• pp.54-59
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• 2017

Objectives: The purpose of this study was to compare the antibacterial activity of urushiol against Enterococcus faecalis (E. faecalis) to that of NaOCl. Materials and Methods: The canals of thirty two single rooted human teeth were instrumented with Ni-Ti files (ProTaper Next X1, X2, X3, Dentsply). A pure culture of E. faecalis ATCC 19433 was prepared in sterile brain heart infusion (BHI) broth. The teeth were submerged in the suspension of E. faecalis and were incubated at $37^{\circ}C$ for 7 days to allow biofilm formation. The teeth were randomly divided into three experimental groups according to the irrigant used, and a negative control group where no irrigant was used (n = 8). Group 1 used physiologic normal saline, group 2 used 6% NaOCl, and group 3 used 10 wt% urushiol solution. After canal irrigation, each sample was collected by the sequential placement of 2 sterile paper points (ProTaper NEXT paper points, size X3, Dentsply). Ten-fold serial dilutions on each vials, and 100 µL were cultured on a BHI agar plate for 8 hours, and colony forming unit (CFU) analysis was done. The data were statistically analyzed using Kruskal-Wallis and Mann-whitney U tests. Results: Saline group exhibited no difference in the CFU counts with control group, while NaOCl and urushiol groups showed significantly less CFU counts than saline and control groups (p < 0.05). Conclusions: The result of this study suggests 10% urushiol and 6% NaOCl solution had powerful antibacterial activity against E. faecalis when they were used as root canal irrigants.

• Proceedings of the KACD Conference
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• 2002.11a
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• pp.720-720
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• 2002

A number of investigations have shown that the presence of bacteria is prerequisite for developing pulpal and/or periradicular pathosis. Depending on the stage of pulpal pathosis, various species of bacteria can be cultured from infected root canals. Kakehashi et al. showed that exposure of pulpal tissue in germ-free rats was characterized by minimal inflammation and dentinal bridging while exposure of pulpal tissue in conventional rats with normal oral flora was characterized by pulpal necrosis, chronic inflammation, and periapical lesions. Currently used methods of cleaning and shaping, especially rotary instrumentation techniques, produce a smear layer that covers root canal walls and the openings to the dentinal tubules. The smear layer contains inorganic and organic substances that include fragments of odontoblastic processes, microorganisms, their by products and necrotic materials. Because of its potential contamination and adverse effect on the outcome of root canal therapy, it seems reasonable to suggest removal of the smear layer for disinfection of the entire root canal system. Presence of this smear layer prevents penetration of intracanal medications into the irregularities of the root canal system and the dentinal tubules and also prevents complete adaptation of obturation materials to the prepared root canal surfaces. Removal of the smear layer by an intracanal irrigant and placement of an antibacterial agent in direct contact with the content of dentinal tubules should allow disinfection of this complex system and better outcome for the root canal therapy. A new solution, which was a mixture of a tetracycline, an acid, and a detergent(MTAD), was developed in the Department of Endodontics, Dental School. Lorna Linda University, USA. It has been demonstrated that MTAD was an effective solution for the removal of the smear layer and does not significantly change the structure of the dentinal tubules when used as a final irrigant in conjunction with 1 % NaOCl as a root canal irrigant. Studies are in progress to compare the anti- microbial properties of this newly developed solution with those of sodium hypochlorite and EDTA that are currently used to irrigate the root canals and remove the smear layer from the surfaces of instrumented root canals.canals.

• Restorative Dentistry and Endodontics
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• v.40 no.2
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• pp.143-148
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• 2015

Objectives: This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI). Materials and Methods: Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant. Results: In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups. Conclusions: NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.

• Restorative Dentistry and Endodontics
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• v.9 no.1
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• pp.107-114
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• 1983

The purpose of this study was to observe the effect of cleansing action of irrigation solutions which was 3% $H_2O_2$ and 5% NaOCl, and 15% EDTA solution on the root canal wall. After treatment with the irrigant, each sample was dehydrated, and coated with 200~250${\circ}$A of gold, and observations were made with the use of scanning electron microscope. The results were as follows: 1. In the root canal walls irrigated with 3% $H_2O_2$ and 5% NaOCl solution without instrumentation after extirpation through barbed broach, the predentin of root canal wall was found scarely affected, and the wall was shown retaining network structure and fibrous organic matters. 2. When 15% EDTA was applied as irrigants for 60, 90 and 120 seconds after instrumentation, there was no signigicant difference of the cleansing effect of the elapsed times which were 90 and 120 seconds on the root canal wall, but in the applied time which was 120 seconds, the canal wall was the cleanest. Therefore it was thought that the most suitable application time of 15% EDTA as the irigants was 120 seconds.

• Restorative Dentistry and Endodontics
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• v.28 no.5
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• pp.425-430
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• 2003

The purpose of this study was to evaluate the clinical applications of the Sodium Dichloroisocyanurate effervescent tablet as a routine root canal irrigant by performing several in vitro tests such as $Cl^{-}$ content. cytotoxicity. antimicrobial effect as well as its pH level compared to the equivalent concentration of sodium hypochlorite solution. 1. Sodium Dichloroisocyanurate demonstrated lower level of $Cl^{-}$ concentration than each dilution of sodium hypochlorite solution. Both solution has increased level of $Cl^{-}$ as the concentration of each solution increased. There was no significant change of $Cl^{-}$ concentration in sodium hypochlorite as time goes by. However. $Cl^{-}$ concentration in Sodium Dichloroisocyanurate was increased. 2. The antimicrobial effects of both solutions were increased when their concentrations were increased. One day after dilution. antimicrobial effect of Sodium Dichloroisocyanurate was slightly higher than sodium hypochlorite. however. there was no difference in 1 week dilution solution. One month dilution solution of sodium hypochlorite still retain its activity. but antimicrobial effect of Sodium Dichloroisocyanurate was drastically decreased 1 month after dilution. 3. The cytotoxicity of Sodium Dichloroisocyanurate was rather higher than same concentration of sodium hypochlorite solution until 1 week after dilution. Then in 1 month. cytotoxicity of Sodium Dichloroisocyanurate was decreased than that of 1 week dilution solution. especially 4% Sodium Dichloroisocyanurate solution has almost no toxicity. However. 1% and 2% sodium hypochlorite solution has unchanged moderate degree of cytotoxicity after the dilution. Furthermore. 4% sodium hypochlorite solution showed high level of toxicity. 4. The pH level of Sodium Dichloroisocyanurate showed that the solution was weak acid (pH5). On the other hand. sodium hypochlorite was revealed as a strong alkaline solution (pH12). There was no change in pH following the dilution of each solution. As results. Sodium Dichloroisocyanurate solution fully satisfy the basic requirements as a root canal irrigation solution. However. we strongly recommend to use this solution clinically in low concentration and try to apply into the root canal within 1 week after dilution.

• Restorative Dentistry and Endodontics
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• v.8 no.1
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• pp.147-153
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• 1982

The purpose of this study is to observe the effect of cleansing action of irrigation solutions which are 3% hydrogen peroxide, 5% sodium hypochlorite and 15% EDTA solution on the root canal wall. After the root canal wall is enlarged with K-file in distilled water, the canal wall which is irrigated with each irrigant for 2 minutes, is compared with the. control group without using any irrigants. Each sample is dehydrated, and coated with 200-250${\AA}$ of gold, and observations are made with the use of scanning electron microscope. The results are as follows: 1. The canal walls irrigated with 3% hydrogen peroxide, 5% sodium hypochlorite and 15% EDTA solution are cleaner than the walls without using irrigants. 2. There are no significant difference of cleansing effect among 3% hydrogen peroxide, 5% sodium hypochlorite and 15% EDTA. 3. After using 3% hydrogen peroxide and 5% sodium hypochlorite solution, large debris are removed on root canal walls, but micro debris remain on the canal walls. 4. The root canal walls irrigated with 15% EDTA solution are decalcifed slightly and show clean surfaces.

• Restorative Dentistry and Endodontics
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• v.7 no.1
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• pp.7-15
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• 1981

This study was conducted to compare the cleansing effect of various irrigants and the function of the quantity of irrigants in apical region. One hundred sixty single rooted teeth were divided into four groups and fourty teeth in each group were individually enlarged and irrigated with each of four irrigants as they would be during clinical condition. The four tested irrigants were Normal saline solution, 3.5% NaOCl, 15% EDTA, and 50% Citric acid. In each group, twenty teeth were flushed with 2cc of each irrigant and another twenty teeth with 5cc of same irrigant after use of each instrument. After final flush and dry, the roots were split longitudinally and the degree of cleansing at apical portion were evaluated under Stereoscope by three observers. The typical specimens of each group were also examined under Scanning electron microscope. The results were as follows. 1. There were no significant difference of cleansing effect among the tested irrigants. 2. 5cc flushed groups have more samples that have high degree of cleansing than 2cc flushed groups. 3. The canals irrigated with 2cc showed the presence of many dentin chips, tissue debris and smeared layer, whereas the 5cc groups revealed relatively clean canal surface and less smeared layer under Scanning electron microscope. 4. The teeth flushed with 5cc of EDTA and with 5cc of Citric acid showed the most clean canal surface at Scanning fictures.

• Restorative Dentistry and Endodontics
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• v.8 no.1
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• pp.115-121
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• 1982

Sodium hypochlorite solution has been widely used as endodontic irrigant due to its ability to dissolve pulp tissue debris and its antimicrobial action. This in vitro study was conducted to evaluate the solvent action of sodium hypochlorite solution on vital pulp tissue under various conditions include concentration, exposure time, and temperature. The percentage of weight loss due to pulp tissue dissolution was calculated with weight difference of lyophilized specimens before and after the exposure to test solutions. The results were as follows; Statistical analysis indicated that the ability of both 5.0% and 2.5% sodium hypochlorite solutions to dissolve pulp tissue was significantly greater than that of distilled water, but no significant difference was found between 5.0% and 2.5% sodium hypochlorite solutions. There was no significant increase in the pulp tissue dissolving ability of sodium hypochlorite solutions; as exposure time increased 2 minutes, 5 minutes, and 10 minutes. Of the given temperatures, no significant difference was found in the solvent aciton of sodium hypochlorite solution on pulp tissue between $20^{\circ}C$ (room temperature) and $37^{\circ}C$(body temperature).

• Proceedings of the KACD Conference
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• 2001.11a
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• pp.574.2-574
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• 2001

The objective of this study was to evaluate the effect of EDTA irrigant depending on application times and temperatures. 31 human mature extracted teeth with a single canal and a closed apex were sectioned with microtome in 3mm thickness. They were distributed randomly into 6 groups of 10 specimens each and control group of 2 specimens. Each specimen was prepared with $GT^{TM}$ rotary file (Dentsply, Maillefer Co., Swiss) and irrigated with 3ml sodium hypochlorite every minute. Then smear layer wasn removed with EDTA solution ($PULPDENT^{\circledR}$, PULPDENT Co., USA.) except two control specimens. Specimens of each group were irrigated with 17％ EDTA.(omitted)

• Restorative Dentistry and Endodontics
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• v.8 no.1
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• pp.77-87
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• 1982

The purpose of this study is to estimate the reaction of rat subcutaneous tissues following exposure to the various concentrations of formocresol (100, 50, 20%), eugenol (100, 75, 50%), and sodium hypochlorite. (5, 3.5, 0.5%) The results were as follows: 1. As the concentration of formocresol was decreased, the inflammatory reaction was decreased conspicuously. 2. The inflammatory reaction of 100% eugenol was appeared to be similar to that of 75% eugenol. The inflammatory reaction of 50% eugenol was decreased conspicuously. 3. No significant differences were found in inflammatory response between 3.5% and 5% sodium hypochlorite. 4. The inflammatory reaction of 0.5% sodium hypochlorite was mild and appeared to be similar to that of saline solution in 7 days.