• Title/Summary/Keyword: irradiation uptake

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Dyeability and Functionality of Chaenomelis Fructus Extract (모과 추출물의 염색성과 기능성)

  • Nam, Ki-Yeon;Lee, Jung-Soon
    • Fashion & Textile Research Journal
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    • v.14 no.3
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    • pp.478-485
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    • 2012
  • The dying properties of fabrics with Chaenomelis Fructus extract were studied through an investigation of the characteristic of Chaenomelis Fructus colorants, the effect of dyeing conditions (dye temperature, dyeing concentration, and times on dye uptakes), effect of mordant, effect of UV irradiation, and color change in addition, antimicrobial ability and deodorant ability were estimated. In the UV-Visible spectrum, the wavelength of maximum absorption of Chaenomelis Fructus extract was 280 nm and showed that tannin is the major pigment component. From the increase of absorbance by UV irradiation, it was assumed that catechol tannin color was developed through UV irradiation. An increased dyeing concentration resulted in a larger dye uptake. Dye uptake increased as the dyeing time and temperature increased. Chaenomelis Fructus extract showed relatively good affinity to silk than cotton. Mordant, Fe and Cu were effective to increase the dye uptake of cotton fabric in addition, the dye uptake of silk fabric mordanted with Fe and K improved. UV irradiation let the color of dyed fabrics develop regardless of the UV irradiation stage however, UV irradiation on the dyed fabric was more effective than on the extract for the color development. Dyed silk fabric showed very good antimicrobial abilities of 99.9% in addition, deodorant ability improved in the fabric dyed with Chaenomelis Fructus extract.

IRRADIATION EFFECT ON SECRETING FUNCTION, AMYLASE ACTIVITY AND NUCLEIC ACID CONTENTS OF RAT PAROTID GLAND (방사선 조사가 이하선 기능에 미치는 영향에 관한 연구)

  • Cho Yong Jin;Park Tae-Won
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.20 no.1
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    • pp.53-62
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    • 1990
  • This experiment was performed to clarify the effects of /sup 60/Co gamma irradiation on secretory function, amylase activity and contents of nucleic acids of parotid gland in rat. Experimental animals were divided into 6th hours, 3rd, 7th, 14th and 28th days after irradiation and control. The experimental animals are singly irradiated with 20Gy (2,000rad) through protective lead block. Secretory function of parotid gland was evaluted by uptake and clearance of /sup 99m/TcO₄. /sup 99m/TcO₄. 0.2μ ci/gm, was injected into peritonium in uptake groups. Rats were sacrified with cervical dislocation after 30 minutes and gland was excised. In the clearance group. pilocarpine nitrate (8㎎/㎏) was intraperitoneally injected at 30 minutes after /sup 99m/TcO₄ injection and rats were sacrified 30 minutes after pilocarpine injection. Radioactivity of excised parotid gland was measured by using of gamma counter and stimulation-secretion coefficients, uptake radioactivity divided by clearance radioactivity, was calculated. Amylase activity and contents of DNA and RNA were determined by spectrophotometry. The results obtained were as follows: 1. In the uptake test, the radioactivity of /sup 99m/TcO₄ per unit weight increase in experimental group except 6th hours group, compared with control groups and showed a peak at 3rd days after irradiation. 2. In the clearance test, the radioactivity of /sup 99m/cO₄per unit weight rose to a peak at 3rd days after irradiation and gradually recovered thereafter. 3. Stimulation-secretion coefficient of parotid gland decreased at 6th hours, 3rd and 7th days after irradiation, and gradually increased. 4. Amylase activity of parotid gland decreased in 3rd and 7th days group, and especially lowest in 3rd days after irradiation. 5. The contents of DNA showed no definite difference in all the experimental groups, but RNA was seemed to decrease with time after irradiation.

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Effects of X-irradiation on the Oxygen Consumption and Lysine Uptake of HeLa Cells in the Presence of Metabolic Substrates and Inhibitors (培養 HeLa 細胞의 酸素消費量과 Lysine 吸收에 미치는 X-線 照射의 影響)

  • Kang, Yung-Sun;Ha, Doo-Bong;Ahn, Kyung-Ja
    • The Korean Journal of Zoology
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    • v.11 no.3
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    • pp.75-82
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    • 1968
  • The effects of x-irradiation on the utilization of glucose, succinate, citrate and $\\alpha$-ketoglutarate, on the response of the cell metabolism to $NaN_3$ and DNP, and on the uptake of lysine in the presence or absence of the metabolitesor the inhibitors were studied using HeLa cells and the results are summarized as follows: 1. 200r of x-irradiation had no immediate effect on the oxygen consumption of cells. 2. The oxygen consumption was greatly stimulated by succinate, $\\alpha$-ketoglutarate and citraed and in decreasing order and x-irradiation caused no remarkable change in this order. 3. The respiratory response of the cell to the metabolic inhibitors seems to be altered by x-irradiation. 4. The initial rate of the uptake of lysine was markedly retarded and the accumulation of lysine in the cell was decreased by irradiation. 5. Glucose increased the lysine uptake whereas succinate had no effect and citrate and $\\alpha$-ketoglutarate reduced the absorption. X-irradiation did not alter this tendency. 6. The inhibitory effects of $NaN_3$ and DNP on the lysine uptake were quite different from those seen in the oxygen consumption.

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Effect of Gamma Irradiation on Water Uptake Rate and Gelatinization of Brown Rice (감마선 조사가 현미의 수화속도 및 호화양상에 미치는 영향)

  • Lee, You-Seok;Shu, Chung-Sik;Lee, Ju-Woon;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.36 no.6
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    • pp.900-904
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    • 2004
  • Effects of gamma irradiation on brown rice quality were evaluated. Brown rice was irradiated at absorbed dose of 1, 3 or 5 kGy, and ground. Water uptake, pasting properties, and physicochemical characteristics of flour samples were tested. Water uptake rates of irradiated samples were higher than that of control, and were dose-dependent. Hydration capacity decreased in sample irradiated at 5 kGy due to leaching out of soluble compounds, whereas no differences were observed among other irradiated samples and control. Irradiation significantly decreased pasting properties as determined by amylograph. Gamma irradiation accelerated water evaporation at high temperatures (over $300^{\circ}C$) in test of weight-loss profile with thermogravimetric analyzer.

Studies on the Cellular Metabolism in Microorganisms as Influenced by Gamma-irradiation.(II) - On the Respiration Rate and Dehydrogenase Actibity in Yeast Cells Irradiated by $\gamma$-ray. (미생물의 세포생리에 미치는 전이방사선의 영향에 관한 연구 (제 2보) - 효모균의 산소호흡기및 탈수소효소능에 대한 $\gamma$-ray 의 영향)

  • 김종협
    • Korean Journal of Microbiology
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    • v.5 no.2
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    • pp.69-78
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    • 1967
  • Kim, Jong Hyup, (Div. of Biology, Atomic Energy Research Institute.) Studies on the Cellular Metabolism in Microorganisms as influenced by Gamma-irradiation(II). On respiration rate and dehydrogenase activity of yeast cells irradiated by gamma ray from cobalt-60. 1. Oxygen uptake rate of the gamma irraiated yeast cells had been measured with Warburg's manometer, and the $O_{2}$-uptake was compared with those of normal cells. The rate of endogetious respiration increases in its $O_2$-uptake at 150, 000 rentgen dose, and at higher rentoen doses it was decreased. Exogenous respiration begin to decrease in its O_2$-uptake at 5, 000r. doses of irradiation, further decrease with increasing of doses unproportionally. 2. It appears that plasma-membrane and nuclear membrane of yeast cells have changed and denatured by gamma-irradiation, as exogenous respiration of glucose had been decreased at a dose of 200, 000r's irradiation. 3. The activity of glucose, alcoholic, lactic, succinic and glutamic deliydrogenase (G.D.H., A.D.H., L.D.H., S.D.11., and GL.D.H.) in the gamma irradaited cells had been assayed by T.T.C.(Triphenyl tetrazolium chloride) method and spectrophotometry, the obtained results were compared with those of normal cells. 4. At a dose of and 10, 000 rentgens' irradiation of gamma ray, the activty of each debydrogenase (G.D.H., A.D.H., L.D.H., ) shows a sharp and highest peak in optical absorbalicy, but each abtivity of S.D.H and Gl.D.H shows its' maximum peak at a dose of 30, 000r. 5. The curve of each dehydrogenase activity was found to be rhythmical according to dose-rate of gamma irradiation. 6. Comparing with activity of debydrogenase each other, the maximum peak in optical absorbency can be arranged according to order as follows; glucose > alcoholoic > lactic > glutamic > succinic, this order is identical to the order of breakdown utility in respiration of normal yeast cells. 7. The activity of dehydrogenase experimented exhibit a resistance against gamma irradiation at lethal dose of cells, and the activity of dehydrogenase are found to be much resistant than those of respiratory system. We may assume that the membrane substrate of mitochondria or cytoplasm had been destructed by gamma-irradiation much more than that of dehydronase system.

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Effects of Gamma-Irradiation on the Water Absorption Property of Black Soybeans (감마선 조사가 검정콩의 수분흡수 특성에 미치는 영향)

  • 김종군
    • Journal of the Korean Home Economics Association
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    • v.30 no.3
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    • pp.101-117
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    • 1992
  • Effects of gamma irradiation(2.5∼20kGy) on water absorption property was studied for a local variety of black soybeans. In water absorption patterns of black soybeans, the time to reach a fixed moisture content was reduced depending on the increment of water soaking temperature and irradiation dose. Irradiation at 2.5∼10kGy resulted in the reduction of soaking time of black soybeans by about 1∼3 hours and the increase of hydration capacity by 10∼20%, respectively, compared to the nonirradiated control black soybean. The water uptake rate constant of the irradiated black soybean difinitely increased with the increase of dose levels and water soaking temperature. The activation energy for water absorption and z-value were lower in the irradiated black soybeans than in the nonirradiated control black soybean. The efficacy of water absorption property in the irradiated black soybeans was also recognized after one year of storage at room temperature.

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Combination Dyeing of Juniperus Chinensis and Terminalia Chebula Retzius (향나무와 가자열매의 혼합 염색)

  • Sa, A-Na;Choi, Hyo Jin;Lee, Jung Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.38 no.3
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    • pp.415-426
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    • 2014
  • This study investigated the color change, enhancement of dye uptake, washing fastness, and light fastness of cotton and silk when dyed with Juniperus chinensis and Terminalia chebula (combination dyeing) using various methods. The dyeing methods were 1) dyeing Juniperus chinensis extract and Terminalia chebula Retzius extract sequentially (J-T), 2) dyeing Terminalia chebula Retzius extract and Juniperus chinensis extract sequentially (T-J), 3) dyeing Juniperus chinensis extract and Terminalia chebula Retzius extract simultaneously (J+T), and 4) dyeing Juniperus chinensis extract 4 times repeatedly and followed by a dyeing Terminalia chebula Retzius extract in sequence. In terms of increased dyeing uptake, combination dyeing (dyeing 2 times like method 1, 2, 3) had the same impact as the repeated dyeing (method 4). The color of J, T combination dyeing showed wide range of mixed YR series, Y series resulting from Terminalia chebula Retzius, and R series resulting from Juniperus chinensis. In cotton, J-T dyeing method showed superior enhancement of dye uptake, and T-J method in silk. In both of the cases, Y series color from Terminalia chebula Retzius extract was mainly shown (Y series color was superior to R series color after dyeing). Therefore, it is recommended for cotton to implement T-J method and for silk to implement J-T method in order to enhance both dyeing uptake and emphasize the color of R series. The K/S value of combination dyed fabrics was decreased by increasing washing time; however, the K/S value of combination dyed fabrics was increased by increasing UV irradiation time. But after repeated washing and longer UV irradiation, ${\Delta}E$ value of combination dyed fabric became smaller compared to fabric dyed with Juniperus chinensis only. Thus, it was found that after J, T combination dyeing, fabric became less prone to fading by washing and UV than after Juniperus chinensis single dyeing was done.

Effects of gamma-irradiation on intracellular proliferation of Toxoplasma gondii RH tachyzoites (톡소포자충 RH tachyzoites에 대한 감마선 조사가 충체의 숙주세포내 증식에 미치는 영향)

  • 국진아;오성희
    • Parasites, Hosts and Diseases
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    • v.33 no.3
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    • pp.173-178
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    • 1995
  • A quantitative assay was performed on the effects of gamma-irradiation (30- 300 Gy) on intracellular proliferation of Toxoplosmn gonnii RH tachyzoites in human leukemic HL-60 cells and murine peritoneal macrophages by means of 3H-uracil uptake assay. Infected non-irradiation group (NI) and uninfected group (incubating only host cells) were prepared. The 3H-uracil uptake by tachyzoites of NI group 12-24 hrs after infection was 2,190-4,787 counts per minute for macrophages and 2,967-8,254 for HL-60 cells, whereas the irradiated tachyzoites revealed only 381-703 (100 Gy) and 218-408 (300 Gy) for macrophages, and 1,911-2,618 (30 Gy), 1,253-1,384 (70 Gyl, 1,013-1,090 (100 Gyl, and 483-588 (300 Gy) for HL-60 cells. The proliferation inhibition rate was similar in macrophages and HL-60 cells, for example, 89-94% and 80-94% respectively by 300 Gy, 12-24 hrs after infection. It is concluded that RH tachyzoites of T gondii are severely affected by gamma-irradiation in their capability of Intracellular proliferation.

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Thallium-201 Uptake and Washout in T1-201 Brain SPECT of Various Brain Tumors (각종 뇌 종양의 Thallium-201 뇌 SPECT에서 Thallium-201의 동태)

  • Lim, Sang-Moo;Hong, Sung-Woon;Rhee, Chang-Hun;Lee, Seung-Hoon;Kim, Jong-Hyun
    • The Korean Journal of Nuclear Medicine
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    • v.26 no.2
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    • pp.360-364
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    • 1992
  • Treatment for the brain tumors consist of surgery, chemotherapy, and a variety of methods of irradiation. Therapy is aimed to destroy the tumor, but necrosis and edema occur concurrently. Conventional structural imaging techniques such as CT or MRI are unable to reliably distinguish persistent and recurrent tumor from necrosis or edema. T1-201 has been shown to be useful in the evaluation of the myocardial viability by comparing the early uptake and redistribution image. The aim of this study is to evaluate the clinical usefulness of the early uptake and delayed washout images of the T1-201 brain SPECT in the brain tumors. In the pathologically diagnosed various brain tumor patients, brain SPECT was done with rotating gamma camera 15 minutes and 3 hours after T1-201 injection, and the T1-201 uptake in the tumor was compared with the skull and scalp activity. In the glioblastoma multiforme, meningioma and metastatic tumor, the T1-201 uptake was higher than low grade glioma in both 15 minute and 3 hour images (p<0.02). In the low grade glioma,3 hour T1-201 uptake was significantly lower than 15 minute uptake (p<0.05) but in the glioblastoma, meningioma and metastatic tumor there was no significant difference. There was no significant difference in the T1-201 uptake among the glioblastoma, meningioma and metastatic tumors. In one matastatic tumor, T1-201 uptake was decreased after radiation therapy. T1-201 brain SPECT could distinguish the benign and malignancy, and seems to be useful in the follow-up after treatment. But one of the early or delayed SPECT seems not to be necessary for these purposes.

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