• 생약학회지
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• 제20권4호
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• pp.219-222
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• 1989

Tectorigenin, irigenin, their glucosides and 5,3'-dihydroxy-4',5'-dimethoxy-6,7-methylenedioxyisoflavone were isolated from the rhizomes of Belamcanda chinensis.

• Food Science and Biotechnology
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• 제14권1호
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• pp.39-41
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• 2005

Four compounds were isolated from the rhizome of Belamcanda chinensis (Iridaceae) by repeated column chromatography and were elucidated as iristectorigenin A, irigenin, tectorigenin and tectoridin by spectral analysis. The estrogenic activity of these isoflavones was tested using Ishikawa cells. Irigenin, tectorigenin and tectoridin were highly estrogenic ($EC_{50}\;=\;0.75$, 0.42 and $0.81\;{\mu}g/ml$, respectively), while iristectorigenin A exhibited weak estrogenic activity ($EC_{50}{\geq}4\;{\mu}g/ml$).

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.202.4-203
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• 2003

The present study was carried out to clarify whether isoflavonoids isolated from Belamcandae Rhizoma (Iridaceae) inhibit inflammation and angiogenesis by the experimental methods in vitro and in vivo. Among the isolated isoflavonoids, such as irigenin, irisflorentine, and iristectorene B inhibited nitric oxide (NO) production, as measured by nitrite formation at 3-30 ${\mu}M$. Also these compounds reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) enzyme expression in a concentration dependent manner, when measured by western blotting, at 3-30 ${\mu}M$. Irigenin, irisflorentine and iristectoren B decreased angiogenesis of chick embryos in the chorioallantoic membrane assay. These compounds also reduced the proliferation of calf pulmonary arterial endothelial (CPAE) cells and found to possess relatively weak gelatinase/collagenase inhibitory activity in vitro. These compounds, when administered subcataneously at the dose of 30mg/kg for 20 days to mice implanted with murine Lewis lung carcinoma (LLC), caused a significant inhibition of tumor volume. Therefore, antiangiogenic activities of isoflavonoids from Belamcandae Rhizoma might be due to antiproliferative activities under inhibition the induction of COX-2 and iNOS enzyme.

• Natural Product Sciences
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• 제10권2호
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• pp.89-91
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• 2004

Six components were isolated from the $CH_2Cl_2$ fraction of Belamcanda chinensis rhizome by open column chromatography. Their structures were elucidated as ${\beta}-sitosterol$ (1), apocynin (2), decursin (3), iristectorigenin A (4), irigenin (5) and tectorigenin (6) by spectral analysis. Among these compounds, decursin (3) was isolated for the first time from a plant of the family Iridaceae.

• Natural Product Sciences
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• 제13권2호
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• pp.101-104
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• 2007

Four isoflavones including tectorigenin (1), irisflorentin (2), irigenin (3), and tectoridin (4) have been isolated from the 70% EtOH extract of Belamcanda chinensis rhizome. The evaluation for protective effect of compounds 1-4 against glutamate-induced cytotoxicity in hippocampal HT22 cell line was conducted. Compound 1 showed significant protective effect with an EC$_{50}$ value of 67.25 ${\pm}$ 1.2 ${\mu}$M, whereas compounds 2-4 were inactive. These results suggest that compound 1 may possess the neuroprotective activity against oxidative cellular injures.

• Archives of Pharmacal Research
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• 제25권3호
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• pp.306-312
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• 2002

Aldose reductase, the key enzyme of the polyol pathway, is known to play important roles in the diabetic complication. The inhibitors of aldose reductase, therefore, would be potential agents for the prevention of diabetic complications. To evaluate active principles for the inhibition of aldose reductase from the rhizomes of Belamcanda chinensis, twelve phenolic compounds were isolated and tested for their effects on rat lens aldose reductase. As a result, isoflavones such as tectorigenin, irigenin and their glucosides were found to show a strong aldose reductase inhibition. Tectoridin and tectorigenin, exhibited the highest aldose reductase inhibitory potency, their $IC_{50}$ values, being $1.08{\times}10^{-6}{\;}M{\;}and{\;}1.12{\times}10^{-6}{\;}M$, respectively, for DL-glyceraldehyde as a substrate. Both compounds, when administered orally at 100 mg/kg for 10 consecutive days to streptozotocin-induced diabetic rats, caused a significant inhibition of sorbitol accumulation in the tissues such as lens, sciatic nerves and red blood cells. Tectorigenin showed a stronger inhibitory activity than tectoridin. From these results, it is suggested that tectorigenin is attributed to be a promising compound for the prevention and/or treatment of diabetic complications.

• 공업화학
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• 제29권6호
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• pp.772-781
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• 2018

본 연구에서는 범부채 뿌리 50% 에탄올 추출물 및 에틸아세테이트 분획물을 제조하고 이들의 항산화 및 항균 활성, 세포 보호 효능을 평가하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) 자유라디칼 소거 활성($FSC_{50}$) 측정 결과, 50% 에탄올 추출물은 $621.5{\mu}g/mL$, 에틸아세테이트 분획물은 $253.0{\mu}g/mL$이었다. Luminol 발광법을 이용한 총 항산화능($OSC_{50}$)은 추출물과 분획물에서 각각 13.6 및 $3.0{\mu}g/mL$이었다. 항균 활성 측정에서 Staphylococcus aureus 및 Candida albicans에 대한 에틸아세테이트 분획물의 최소저해농도(minimum inhibitory concentration, MIC)는 각각 156 및 $1,250{\mu}g/mL$으로 나타났으며, 화장품에 사용하는 기존 방부제인 methyl paraben보다 유사하거나 더 높은 활성을 보여주었다. $^1O_2$로 유도된 세포 손상에 대한 보호 효과(${\tau}_{50}$)에서 50% 에탄올 추출물은 $4{\sim}64{\mu}g/mL$ 농도 범위에서 농도 의존적으로 세포 보호 활성을 나타냈다. $16{\mu}g/mL$ 농도에서 50% 에탄올 추출물, 에틸아세테이트 분획물 및 (+)-${\alpha}$-tocopherol의 ${\tau}_{50}$은 각각 36.4, 45.0 및 45.8 min이었으며, 에틸아세테이트 분획물은 $^1O_2$로 유도된 세포 손상에서 (+)-${\alpha}$-tocopherol과 유사한 세포 보호 활성을 나타냈다. UVB로 유도된 HaCaT 세포 손상에서 에틸아세테이트 분획물은 $8{\mu}g/mL$에서 세포 내 활성산소종(reactive oxygen species, ROS)을 최대 45.9%까지 감소시켰다. 과산화수소로 유도된 HaCaT 세포 손상에서도 에틸아세테이트 분획물은 $0.5{\sim}8.0{\mu}g/mL$에서 세포 생존율을 유의적으로 증가시켰다. 범부채 뿌리 에틸아세테이트 분획물의 성분 분석 결과, irisflorentin, irigenin, tectorigenin, resveratrol, iridin 및 tectoridin 등의 플라보노이드 및 폴리페놀 성분이 확인되었다. 결론적으로 범부채 뿌리 추출물 및 분획물은 화장품의 천연 항산화 및 항균 소재로서 적용 가능성이 있음을 시사한다.