• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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• pp.325-328
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• 2005

To estimate presumptive local ground subsidence area near Abandoned Under ground Coal Mine(AUCM) at Samcheok city in Korea, the geological properties of existing ground subsidence area and the geophysical prospecting data were analyzed using GIS. The electrical resistivity survey and seismic reflection survey database were constructed from investigation reports and factors which are related with ground subsidence such as geological map, topological map, land use map, lineament map, groundwater level, RMR (Rock Mass Rating), mining tunnel map and slope database were constructed also to make a comparative study of each parameters. As a result of the spatial analysis of existing ground subsidence area, 9 major factors causing ground subsidence were extracted and a connection between the structure of underground and the ground subsidence was determined from the analysis of geophysical prospecting data. The estimation of presumptive ground subsidence area was performed using the correlation between the result from neural network analysis of 9 factors and the scrutiny of geophysical prospecting data.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 추계 학술발표회
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• pp.1274-1281
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• 2010

Cut Slope Management System(CSMS) is a systematic maintenance and management system designed to prevent the collapse of cut slopes located along national roads and to minimize damages caused by slope collapse related accidents. In order to implement a maintenance and management system that will ensure safe road operation, the Korean government has started the inventory of cut slopes and developed a cut slope database since 2006. The study on the application of cut slope database is on-going and part of this study is the development of cut slope database management system (Slope-navi) using GIS technology and navigation system for the effective and efficient cut slope management using Information Technology. Using the Navigation Cut Slope Data Management System the previously developed database was checked and verified. The converted cut slope inventory data and field investigation reports of about 20,000 cut slopes were loaded in the navigation map. Through the development of the navigation's cut slope data search system, a systematic and efficient cut slope database management and operation in the prevention of slope collapse and remedial measure selection was established.

• BMB Reports
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• 제28권2호
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• pp.129-137
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• 1995

The ryanodine-receptor $Ca^{2+}$ release channel protein in the sarcoplasmic reticulum membrane of rabbit skeletal muscle plays an important role in muscle exitation-contraction (E-C) coupling. Various types of detergents were tested, including Chaps, cholate, octylglucoside, Zwittergents, Mega-9, Lubrol PX, and Triton X-100 for solubilization of this protein. Among these, Chaps and Triton X-100 were found to optionally solubilize the channel complex. Optimum conditions for this solubilization were pH 7.4 with a salt concentration of 1 M. The addition of phospholipid in the solubilization step helped in stabilizing the protein. The purification of the receptor was performed using sucrose density gradient centrifugation. Various methods [dilution, freeze-thaw, adsorption (Biobeads), and dialysis] were investigated to incorporate the Chaps-solubilized and purified $Ca^{2+}$ release channel protein into liposomes made from different types of phospholipids. Of these, a combined method consisting of a dialysis, freeze-thaw and sonication steps yielded the best results. Reconstituted vesicles produced by this method with 95% phosphatidylcholine (from soybean extract) had good function.

• BMB Reports
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• 제44권4호
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• pp.217-231
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• 2011

T cell exhaustion develops under conditions of antigen-persistence caused by infection with various chronic pathogens, such as human immunodeficiency virus (HIV) and myco-bacterium tuberculosis (TB), or by the development of cancer. T cell exhaustion is characterized by stepwise and progressive loss of T cell function, which is probably the main reason for the failed immunological control of chronic pathogens and cancers. Recent observations have detailed some of the intrinsic and extrinsic factors that influence the severity of T cell exhaustion. Duration and magnitude of antigenic activation of T cells might be associated with up-regulation of inhibitory receptors, which is a major intrinsic factor of T cell exhaustion. Extrinsic factors might include the production of suppressive cytokines, T cell priming by either non-professional antigenpresenting cells (APCs) or tolerogenic dendritic cells (DCs), and alteration of regulatory T (Treg) cells. Further investigation of the cellular and molecular processes behind the development of T cell exhaustion can reveal therapeutic targets and strategies for the treatment of chronic infections and cancers. Here, we report the properties and the mechanisms of T cell exhaustion in a chronic environment.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2001년도 가을 학술발표회 논문집
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• pp.879-884
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• 2001

The use of recycled-aggregate concrete is increasing faster than the development of appropriate design recommendations. In addition, recycled-aggregate and higher compressive strengths are two of the most desired characteristics to improve the use of concrete as a construction material. The paper reports limited experimental data on the shear capacity of high-strength recycled aggregate concrete beams. Ten beams were tested to determine their diagonal cracking and ultimate shear capacities. The variable in the test program were concrete strength(300, 500 and 700kgf/$cm^{2}$), and shear span/depth ratio (a/d : 2.0, 3.0 and 4.0). Test results indicate that the ACI Building code prediction of Eq.(11-3) and (11-5) for high-strength recycled aggregate concretes are unconservative for all beams (with concrete strength 300, 500 and 700kgf/$cm^{2}$, a/d ratios 2.0, 3.0 and 4.0). But Zsutty Equation for high-strength recycled aggregate concretes is conservative for all beams. The results of the experimental investigation on the cracking patterns for beams show that the angle that the critical inclined crack makes with the horizontal axis decreases with increasing a/d.

• International Journal of Concrete Structures and Materials
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• 제6권4호
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• pp.239-246
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• 2012

Pervious concrete is a tailored-property concrete with high water permeability which allow the passage of water to flow through easily through the existing interconnected large pore structure. This paper reports the results of an experimental investigation into the development of pervious concrete with reduced cement content and recycled concrete aggregate for sustainable permeable pavement construction. High fineness ground granulated blast furnace slag was used to replace up to 70 % cement by weight. The properties of the pervious concrete were evaluated by determining the compressive strength at 7 and 28 days, void content and water permeability under falling head. The compressive strength of pervious concrete increased with a reduction in the maximum aggregate size from 20 to 13 mm. The relationship between 28-day compressive strength and porosity for pervious concrete was adversely affected by the use of recycled concrete aggregate instead of natural aggregate. However, the binder materials type, age, aggregate size and test specimen shape had marginal effect on the strength-porosity relationship. The results also showed that the water permeability of pervious concrete is primarily influenced by the porosity and not affected by the use of recycled concrete aggregate in place of natural aggregate. The empirical inter-relationships developed among porosity, compressive strength and water permeability could be used in the mix design of pervious concrete with either natural or recycled concrete aggregates to meet the specification requirements of compressive strength and water permeability.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2002년도 춘계학술대회 논문집
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• pp.125-129
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• 2002

Authors had developed the model for the fatigue life assessment of welded details considering residual stress and its relaxation. The model consists of three ingredients; a hot-spot stress approach, a residual stress relaxation, and an equivalent stress. The equivalent stress is induced by stress ranges and the ratios between the applied mean stresses and the ultimate stress of material. Once being tuned with two specific fatigue tests by using load carrying cruciform joint, this model can be applied to many kinds of welded details which structural stress concentration factors are different from each other. This paper reports the application of the proposed model for various welded details including cover plate, longitudinal stiffener, gusset and side attachment. From the investigation of predicted results by using the proposed model it was shown that the ambiguous fatigue characteristics of the various details influenced widely by the welding residual stress are clarified, and also the model could be applied to assess fatigue life of general welded structures.

• Journal of Nutrition and Health
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• 제36권9호
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• pp.966-973
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• 2003

The purpose of this study was to investigate the utilization level of forecasting methods in contract foodservice management companies. Questionnaires were distributed and collected from 30 foodservice management companies contracted with universities and 49 university foodservices in Seoul and Kyungki area. Statistical data analysis was performed using SPSS/WIN 10.0 based on the production records of Yonsei University foodservices and the weather reports from a meteorological observatory. The results of this study were as follows: 1) The objectives of the fore-casting systems were identified as saving costs through eliminating the leftover, meeting the customer demands, and improving efficiency in food preparation.2) All of the university foodservices were already performing the forecasting methods but in foodservice management companies as a whole,89.7 percents were applying the method and only 55.2 percents had the separate forecasting department. 3) A large number of foodservice staffs in the head office (65.5％) answered that they often utilized intuitive estimates based on the past experiences and records for forecasting while 65.3% managing staffs in the university foodservices answered the same.4) Both in the head office and university foodservices, actual number of meals served were recorded. In the head office, mostly estimated numbers and actual numbers of meals were recorded while estimated, prepared, and actual numbers of meals served were recorded for most of the cases in university foodservices. 5) The primary factors considered for forecasting were the actual production records for the last month, the customer preference for the selected menu items, and the specific day of the week.

• BMB Reports
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• 제32권5호
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• pp.429-435
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• 1999

Unlike the mammalian glucose transporter GLUT1, little is known about the nature of the endogenous sugar transporter(s) in insect cells. In order to establish the transport characteristics and other properties of the sugar transport proteins of Sf9 cells, a series of kinetic analyses was performed. A saturable transport system for hexose uptake has been revealed in the insect cells. The apparent affinity of this transport system(s) for 2-deoxy-D-glucose was relatively high, the $K_m$ for uptake being <0.5 mM. To further investigate the substrate and inhibitor recognition properties of the insect cell transporter, the ability of other sugars or drugs to inhibit 2-deoxy-D-glucose transport was examined by measuring inhibition constants ($K_j$). Transport was inhibited by D-mannose, D-glucose, and D-fructose. However, the apparent affinity of the C-4 epimer, D-galactose, for the Spodoptera transporter was relatively low, implying that the hydroxyl group at the C-4 position may play a role in the strong binding of glucose and mannose to the transporter. The results also showed that transport was stereoselective, being inhibited by D-glucose but not by L-glucose. It is therefore concluded that insect cells contain an endogenous glucose transport activity that in several aspects resembles the human erythrocyte glucose transporter. However, the mammalian and insect transporters were different in some of their kinetic properties, namely, their affinities for fructose and for cytochalasin B.

• BMB Reports
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• 제31권4호
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• pp.307-327
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• 1998

Cytochrome c peroxidase (CcP) is a yeast mitochondrial enzyme which catalyzes the reduction of hydrogen peroxide to water using two equivalents of ferrocytochrome c. The CcP/cytochrome c system has many features which make it a very useful model for detailed investigation of heme protein structure/function relationships including activation of hydrogen peroxide, protein-protein interactions, and long-range electron transfer. Both CcP and cytochrome c are single heme, single subunit proteins of modest size. High-resolution crystallographic structures of both proteins, of one-to-one complexes of the two proteins, and a number of active-site mutants are available. Site-directed mutagenesis studies indicate that the distal histidine in CcP is primarily responsible for rapid utilization of hydrogen peroxide implying significantly different properties of the distal histidine in the peroxidases compared to the globins. CcP and cytochrome c bind to form a dynamic one-to-one complex. The binding is largely electrostatic in nature with a small, unfavorable enthalpy of binding and a large positive entropy change upon complex formation. The cytochrome c-binding site on CcP has been mapped in solution by measuring the binding affinities between cytochrome c and a number of CcP surface mutations. The binding site for cytochrome c in solution is consistent with the crystallographic structure of the one-to-one complex. Evidence for the involvement of a second, low-affinity cytochrome c-binding site on CcP in long-range electron transfer between the two proteins is reviewed.