• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.1726-1729
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• 1997

In general, the solvability of linear robust output regulation problem are not preserved under time-sampling. Thus, it is found that the digital regulator implemented by itme-sampling of anlog output regulator designed based on the continuous-time linear system model is nothing but a 1st order approximation with respect to time-sampling. By the way, one can design an improved sampled-data regulator with respect to sampling time by utilizing the intrinsic structure of the system. In this paper, we study the system structures which it is possible to design an improved sampled-data regulator with respect to sampling time.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.10
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• pp.85-93
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• 1998

In general, the solvability of linear robust output regulation problem are not preserved under time-sampling. Thus, it is found that the digital regulator implemented by time-sampling of analog output regulator designed based on the continuous-time linear system model is nothing but a 1st order approximation with respect to time-sampling. However, one can design an improved sampled-data regulator with respect to sampling time by utilizing teh intrinsic structure of the system. In this paper, we study the system structures for which it is possible to design an improved sampled-data regulator with respect to sampling time.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.15 no.4
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• pp.315-323
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• 1990

A design technique of dynamic stabilization controller for the intrinsic unstable inverted pendulum system is introduced. Mathematical modelling with the more complex nonlinearity and the stabilized control theory presented by C.D.Johnson are adapted to this system by using the state-space approach. And the Stabilized controller with the designed optimal regulator type which can be fastly tracked and can be accurately counteracted aginst all effects of the constant distrubances and the parameteric variations is simulated and is implemeted successfully on the microcomputer.

• The Korean Journal of Pain
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• v.19 no.1
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• pp.8-16
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• 2006

The regulators of the G protein signaling (RGS) proteins are responsible for the rapid acceleration of the GTPase-activity intrinsic to the heterotrimeric G protein alpha subunits. As GTPase-activating proteins (GAP), the RGS proteins negatively regulate the G-protein signals. Recently, the RGS proteins are known to be one of the important regulators of opioid signal transduction and the development of tolerance. The aim of this study was to review the recent discovery and understanding of the role of RGS proteins in opioid signaling and the development of tolerance. This information will be useful for medical personnel, particularly those involved in anesthesia and pain medicine, by helping them improve the effective use of opioids and develop new drugs that can prevent opioid tolerance.

• Mass Spectrometry Letters
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• v.6 no.2
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• pp.31-37
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• 2015

Phosphorylation upon protein is well known to a key regulator that implicates in modulating many cellular processes like growth, migration, and differentiation. Up to date, grafting of multidimensional separation techniques onto advanced mass spectrometry (MS) has emerged as a promising tool for figuring out the biological functions of phosphorylation in a cell. However, advanced MS-based phosphoproteomics is still challenging, due to its intrinsic issues, i.e., low stoichiometry, less susceptibility in positive ion mode, and low abundance in biological sample. To overcome these bottlenecks, diverse techniques (e.g., SCX, HILIC, ERLIC, IMAC, TiO₂, etc.) are continuously developed for on-/off-line enrichment of phosphorylated protein (or peptide) from biological samples, thereby helping qualitative/quantitative determination of phosphorylated protein and its phosphorylated sites. In this review, we introduce to the overall views of enrichment tools that are universally used to selectively isolate targeted phosphorylated protein (or peptide) from ordinary ones before MS-based phospoproteomic analysis.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.182.2-182.2
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• 2012

If the satellite has the magnetic material or magnetic moment, the satellite is affected by the earth magnetic field by the space environment in Geostational orbit. The aim of this paper is to assess the satellite magnetic momentum which is an input to ADCS(Altitude Determination Control Subsystem) analyses to assess spurious torques. The magnetic momentum at satellite level is due to magnetic momentum generated by each unit which is due to internal currents circulation or to the presence of magnetic components. Also the magnetic momentum at satellite level is due to circulation of the DC supply current from PSR(Power Supply Regulator) to each unit. As introducing the intrinsic contribution of each unit and the magnetic moment based on the current return through the structure, this paper assess the satellite magnetic moment.

• Development and Reproduction
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• v.21 no.4
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• pp.449-456
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• 2017

The germline stem cells of the Drosophila ovary continuously produce eggs throughout the life-span. Intricate regulation of stemness and differentiation is critical to this continuous production. The translational regulator Nos is an intrinsic factor that is required for maintenance of stemness in germline stem cells. Nos expression is reduced in differentiating cells at the post-transcriptional level by diverse translational regulators. However, molecular mechanisms underlying Nos repression are not completely understood. Through three distinct protein-protein interaction experiments, we identified specific molecular interactions between translational regulators involved in Nos repression. Our findings suggest a model in which protein complexes assemble on the 3' untranslated region of Nos mRNA in order to regulate Nos expression at the post-transcriptional level.

• Development and Reproduction
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• v.27 no.4
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• pp.221-226
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• 2023

A stem cell niche provides an environment that governs stem cell maintenance and division. Thus, the development of a proper niche is of prime importance to stem cell behaviors. Mechanisms of niche development are beginning to be revealed in the Drosophila male gonad. Niche cells are initially dispersed throughout the gonad, then assemble at its apical tip through the anterior migration of posteriorly located niche cells. The molecular mechanisms of this migration and assembly are still poorly understood. Here we show evidence suggesting that Lin28, an RNA-binding protein and regulator of let7 genesis, might be an intrinsic factor for the anterior migration of niche cells. We found that a dispersed, ectopic niche, a phenotype observed with anterior migration defects, occurs in lin28 mutant gonads. This phenotype is rescued by expression of lin28 in the niche cells. These findings suggest that Lin28 might be required for the anterior migration of niche cells.

• Korean Journal of Microbiology
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• v.46 no.1
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• pp.104-108
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• 2010

We describe the construction of derivatives of wild type and mutant lrp genes that encode 6XHis-tag Lrps. These derivatives of wild type and mutant Lrp could be useful for in vitro studies including Lrp conformational changes. We show that 6XHis-tag Lrp wild type and 6XHis-tag Lrp R145W bind with similar patterns in vitro to 21 bp duplex DNA containing the consensus sequences of Lrp sites of upstream of the ilvIH operon. In addition, we report here the 6XHis-tag Lrp R145W is useful to investigate the conformational changes of Lrp in solution by using its own intrinsic fluorescence characteristics.

• Molecules and Cells
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• v.38 no.1
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• pp.81-88
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• 2015

Flowering time (or heading date) is controlled by intrinsic genetic programs in response to environmental cues, such as photoperiod and temperature. Rice, a facultative short-day (SD) plant, flowers early in SD and late in long-day (LD) conditions. Casein kinases (CKs) generally act as positive regulators in many signaling pathways in plants. In rice, Heading date 6 (Hd6) and Hd16 encode $CK2{\alpha}$ and CKI, respectively, and mainly function to delay flowering time. Additionally, the major LD-dependent floral repressors Hd2/Oryza sativa Pseudo-Response Regulator 37 (OsPRR37;hereafter PRR37) and Ghd7 also confer strong photoperiod sensitivity. In floral induction, Hd16 acts upstream of Ghd7 and CKI interacts with and phosphorylates Ghd7. In addition, Hd6 and Hd16 also act upstream of Hd2. However, whether CKI and $CK2{\alpha}$ directly regulate the function of PRR37 remains unclear. Here, we use in vitro pull-down and in vivo bimolecular fluorescence complementation assays to show that CKI and $CK2{\alpha}$ interact with PRR37. We further use in vitro kinase assays to show that CKI and $CK2{\alpha}$ phosphorylate different regions of PRR37. Our results indicate that direct posttranslational modification of PRR37 mediates the genetic interactions between these two protein kinases and PRR37. The significance of CK-mediated phosphorylation for PRR37 and Ghd7 function is discussed.