• 한국작물학회지
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• 제41권5호
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• pp.608-618
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• 1996

Glycine속의 야생 다년생종인 G. tomentella가 가지고 있는 내병성, 고단백 및 다년생 유전자등 유용형질들을 재배콩(G. max)에 도입하기 위한 종간교잡시 염색체수가 다른 4개의 G. tomentella (2n=38, 40, 78, 80) 계통들과 재배콩과의 교잡친화성 검정, 정역간 교잡효과, 교잡엽의 발육비교, 종간 교배적기 등에 대해 시험한 결과를 요약하면 다음과 같다. 1. G. tomentella의 염색체수가 2n=38, 40, 78, 80인 4개 계통과 재배콩인 울산콩, 뱀콩, 단엽콩과의 종간 교잡에서 G. tomentella의 염색체수가 2n=38인 계통과 교배된 것이 교배율 및 교배후 경과일수에 따른 협생존률이 가장 양호하여 2n=38계통이 재배콩과의 교잡친화성이 가장 높은 것으로 나타났다. 2. G. max(cv. Baemkong)와 G. tomentella(2n=38)와 종간교잡에서 정역교잡 효과는 G. max를 모본으로 했을 때가 G. tomentella를모본으로 했을 때보다 교배율이 더 높았고, 엽생존기간도 더 길어 정역교잡의 효과가 인정되었다. 3. G. max(cv. Baemkong)와 G. tomentella(2n=38)와의 종간교잡에서 교잡협의 발육상태를 자가수정된 모본의 협과 비교했을 때 G. max를 모본으로 한 것이 G. tomentella를 모본으로 한 것보다 협당 배주수의 감소율이 적었으나, 교배후 경과일수에 따른 협의 장, 폭 및 무게는 G. max를 모본으로 한 경우가 생육이 저조하였다. 4. 자연조건에서 G. max와 G. tomentella(2n=38)와의 종간 교잡을 할 경우 6월 이나 7월에 하는 것보다 대체로 온도와 습도가 높고 단일조건인 8월 중하구에 하는 것이 교배율이 높았다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.303-304
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• 2001

The family Ulvaceae species are difficult to distinguish from one another on the basis of morphological and cytological criteria alone. ITS2 sequences are hewn to evolve quickly and have been reported to be useful for the study of intraspecific and interspecific variation and biogeography in algae (Bakker et al., 1992). We will here describe the basic characteristics of the ITS2 sequences in Ulva and Effteromorpha to compare our result with the above previous studies. (omitted)

• 생명과학회지
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• 제10권6호
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• pp.598-604
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• 2000

This study was conducted to investigate the effect of simulated acid rain (SAR) on germination, growth, acid buffering capacity and nutrient leaching in Impatiens balsamina L. and Tagetes patula L.. In both species, germination was not inhibited at pH 4.0, but the germination rate decreased at the lower pH of 3.0, showing higher rate in Inpatiens balsamina L. than Tagetes patula L.. As the pH decreases, the growth of radicle was markedly decreased than that of hypocotyl in both species. The plant height, root length, leaf area, total dry weight, relative growth rate and net assimilation rate were inhibited by SAR. The acid buffering capacity in the leaves were increased at pH 4.0, on the other hand, it was shown a tendency to decrease at pH 2.0 in both species. As the pH levels decreased from 5.6 to 2.0, the nutrient leaching from leaves was significantly increased in both species. Based on the results, there are a great difference in the responses to SAR between the two species. In general, Tagetes patula L. represented a higher tolerance to SAR than Impatiens balsamina L.. These results suggested that interspecific variation in the acid buffering capacity and nutrient leaching from leaves may be responsible for the interspecific susceptibility to SAR.

• Journal of Forest and Environmental Science
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• 제33권3호
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• pp.197-201
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• 2017

Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

• 한국산림과학회지
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• 제87권2호
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• pp.194-200
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• 1998

천연림내의 참나무류와 자연 수분된 종자 및 인공적인 종간 교잡 종자를 양묘해서 키운 묘목의 종 및 잡종 간의 개아기(開芽期) 차이를 조사하였다. 금산연습림(禁山演習林)의 천연림에서 개아(開芽)는 Q. crispula > Q. anguste-lepidota > Q. takatorensis ${\fallingdotseq}$ Q. serrate > Q. dentata 순으로 진행되었다. 자연 수분된 종자로 양묘한 묘목에서는 Q. aliena ${\fallingdotseq}$ Q. crispula > Q. anguste-lepidota > Q. takatorensis ${\fallingdotseq}$ Q. serrate > Q. dentata 순으로 개아(開芽)하였다. 종간 교잡의 묘목에서 $F_1$의 개아기(開芽期)는 양친종의 사이에 속했지만, 개아기(開芽期)가 빠른 양친의 영향을 보다 크게 받는 것으로 나타났다. 종간교잡의 결과를 통해서 추정할때 참나무류의 개아기(開芽期)의 빠르고 느린 특성은 유전적인 지배를 받아, 후대에 유전되는 것으로 사료된다.

• Molecules and Cells
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• 제23권2호
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• pp.220-227
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• 2007

The development of suitable genetic markers would be useful for defining species and delineating the species boundaries of morphologically indistinguishable sponges. In this study, genetic variation in the sequences of nuclear rDNA and the mitochondrial cytochrome c oxidase subunit 1 and 3 (CO1 and CO3) regions were compared in morphologically indistinguishable Korean Halichondriidae sponges in order to determine the most suitable species-specific molecular marker region. The maximal congeneric nucleotide divergences of Halichondriidae sponges in CO1 and CO3 are similar to those found among anthozoan cnidarians, but they are 2- to 8-fold lower than those found among genera of other triploblastic metazoans. Ribosomal internal transcribed spacer regions (ITS: ITS1 + ITS2) showed higher congeneric variation (17.28% in ITS1 and 10.29% in ITS2) than those of CO1 and CO3. Use of the guidelines for species thresholds suggested in the recent literature indicates that the mtDNA regions are not appropriate for use as species-specific DNA markers for the Halichondriidae sponges, whereas the rDNA ITS regions are suitable because ITS exhibits a low level of intraspecific variation and a relatively high level of interspecific variation. In addition, to test the reliability of the ITS regions for identifying Halichondriidae sponges by PCR, a species-specific multiplex PCR primer set was developed.

• Animal Systematics, Evolution and Diversity
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• 제35권1호
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• pp.37-39
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• 2019

We determined the cytochrome c oxidase subunit 1 (CO1) sequences of Nebalia koreana Song, Moreira & Min, 2012 (Leptostraca) collected from five locations in South Korea, and this represents the first genetic data of this species. The maximum intra-species variation was 1.2% within Nebalia hessleri Martin, Vetter & Cash-Clark, 1996, while inter-species variation ranged from 9.0% (N. hessleri and Nebalia gerkenae Haney & Martin, 2000) to 34.8% (N. hessleri and Nebalia pseudotroncosoi Song, Moreira & Min, 2013). This result is well agreed with the interspecific relationships among Nebalia species based on morphological characteristics. In conclusion, this study showed the usefulness of CO1 sequences as a DNA barcode within the genus Nebalia Leach, 1814.

• 한국육종학회지
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• 제41권4호
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• pp.463-467
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• 2009

Allium 속 근연종인 파와 양파간 종간교잡을 이용하여 양파로부터 새로운 형질을 도입하는 과정에서 유래되는 종간교잡 F1과 여교잡 세대들의 게놈크기의 변화를 flow cytometry를 이용하여 측정한 결과 다음과 같은 결과를 얻었다. 1. 양파와 파의 종간교잡 F1의 식물학적 특성은 양친의 중간형을 보였으나 여교잡이 진전됨에 따라 반복친인 파의 표현형이 우세하였다. flow cytometry를 이용하여 2C nuclear DNA content을 측정한 결과 $F_1$은 양친에 비해 오히려 증가하였으나 여교잡이 진행됨에 따라 급속히 감소하여 $BC_2F_1$에서는 반복친과 비슷한 수준으로 감소하였다. 여교잡세대의 자식인 $BC_1F_2$은 반복친과 비슷한 $23.1{\pm}1.0pg$으로 측정되었으나 $BC_2F_2$는 $22.9{\pm}0.4pg$으로 반복친의 2C nuclear DNA content인 $23.2{\pm}0.2pg$ 보다 오히려 감소하였다.

• Animal Systematics, Evolution and Diversity
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• 제31권3호
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• pp.160-175
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• 2015

The echolocation calls of bats can provide useful information about species that are generally difficult to observe in the field. In many cases characteristics of call structure can be used to identify species and also to obtain information about aspects of the bat's ecology. We describe and compare the echolocation call structure of 14 of the 21 bat species found in Korea, for most of which the ecology and behavior are poorly understood. In total, 1,129 pulses were analyzed from 93 echolocation call sequences of 14 species. Analyzed pulses could be classified into three types according to the pulse shape: FM/CF/FM type, FM type and FM/QCF type. Pulse structures of all species were consistent with previous studies, although geographic variation may be indicated in some species. Overall classification rate provided by the canonical discriminant analysis was relatively low. Especially in the genera Myotis and Murina, there are large overlaps in spectral and temporal parameters between species. On the other hand, classification rates for the FM/QCF type species were relatively high. The results show that acoustic monitoring could be a powerful tool for assessing bat activity and distribution in Korea, at least for FM/QCF and FM/CF/FM species.

• ALGAE
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• 제17권3호
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• pp.135-144
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• 2002

Scytosiphonaceae is an acetocarpalean brown algal family, that is a recent focus of synstematics and marine biodiversity. We describe Petalonia zosterifolia and Scytosiphon gracilis from Korea for the first time. P. zosterifolia occurred on the East coast, and had flat, linear and solid thalli. S. gracilis was found in Jeju, and had cylindircal to flat and hol-low thalli. However, these two species are so similar that it is difficult to identify by morphology alone. In order to determine if the nuclear DNA reveals the distinctness of both species and to know their phylogenies, the ITS region sequences were newly detrmined in 22 samples of P. zosterifolia, Scytosiphon gracilis, and other three members of the genera from Korea. We found 0.12% variation among samples of P. zosterifolia from different locations, and no variation between S. gracilis samples from diferent years, but extensive interspecific divergences (13.62-22.83%) of each species to other members in Petalonia and Scytosiphon . The ITS sequence dta consistently showed a close relationship between P. zosterifolia and S. gracilis. This result is congruent with morphology and with the published data of plastid rbc and partial nrDNA large subunit gene sequences, and suggests that P. zosterifolia and S. gracilis might have diverged from the most recent common ancestor.