• 제목/요약/키워드: interspecific relationship

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Utility of Selected Non-coding Chloroplast DNA Sequences for Lineage Assessment of Musa Interspecific Hybrids

  • Swangpol, Sasivimon;Volkaert, Hugo;Sotto, Rachel C.;Seelanan, Tosak
    • BMB Reports
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    • 제40권4호
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    • pp.577-587
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    • 2007
  • Single-copy chloroplast loci are used widely to infer phylogenetic relationship at different taxonomic levels among various groups of plants. To test the utility of chloroplast loci and to provide additional data applicable to hybrid evolution in Musa, we sequenced two introns, rpl16 and ndhA, and two intergenic spacers, psaA-ycf3 and petA-psbJ-psbL-psbF and combined these data. Using these four regions, Musa acuminata Cola(A)- and M. balbisiana Colla (B)-containing genomes were clearly distinguished. Some triploid interspecific hybrids contain A-type chloroplasts (the AAB/ABB) while others contain B-type chloroplasts (the BBA/BBB). The chloroplasts of all cultivars in 'Namwa' (BBA) group came from the same wild maternal origin, but the specific parents are still unrevealed. Though, average sequence divergences in each region were little (less than 2%), we propose that petA-psbJ intergenic spacer could be developed for diversity assessment within each genome. This segment contains three single nucleotide polymorphisms (SNPs) and two indels which could distinguish diversity within A genome whereas this same region also contains one SNP and an indel which could categorize B genome. However, an inverted repeat region which could form hairpin structure was detected in this spacer and thus was omitted from the analyses due to their incongruence to other regions. Until thoroughly identified in other members of Musaceae and Zingiberales clade, utility of this inverted repeat as phylogenetic marker in these taxa are cautioned.

Allium속 이배체 2종과 사배체 2종의 Giemsa C-분염핵형 (Giemsa C-banded Karyotypes in Two Diploid and Two Tetraploid Allium Species)

  • 서봉보
    • Journal of Plant Biology
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    • 제32권3호
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    • pp.181-188
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    • 1989
  • C-banded karyotypes in A. anisopodium, A. sacculiferum, A. deltoide-fistulosum and A. splendens were investigated. The chromosome compositions were diploid of 2n=16 in A anisopodium and A. splendens, and teiraploid of 2n=32 in a. sacculiferum and A. deltoide-fistulosum. The bands were distributed in telomeric parts of the chromosomes dominantly in addition to interstitial regions sporadically, resulting in the specific C-banded karyo types according to the species. No centromeric band was observed in these species except only one chromosome in A. deltoidefistulosum. The interspecific relationship based on the C-band distribution will be discussed.

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로티퍼, Brachionus rotundiformis와 코페포다, Tigsiopus japonicus의 혼합 배양조에 있어서 두 종간의 상호 관계 (Interspecific relationship between two food organisms in the combination culture tank of rotifer, Brachionus rotundiformis and copepod, Tigriopus japonicus)

  • 정민민;노섬;김형신
    • 한국수산과학회지
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    • 제33권1호
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    • pp.66-69
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    • 2000
  • 이 연구에서는 로티퍼 B. rotundiformis의 대량 배양조에서 흔히 혼재가 관찰되는 코페포다 T japonicus가 배양을 목적으로 하고 있는 로티퍼 B. rotundiformis의 증식에 어떠한 영향을 미치는지 알아보았다. 실험에 사용한 로티퍼와 코페포다는 로티퍼의 대량 배양조에서 순수 분리 후 단일종 배양하던 것이다. 배앙 조건은 현장의 배양환경과 되도록 일치하도록 하였으며, 먹이는 $7{\times}10^5\;cells/ml$의 N. oculata를 급이하였다. B. rotundiformis의 배양조에 혼재된 T. japonicus는 C. rotundiformis를 단독 배양한 경우에 비하여 증식이 크게 억제되었고, 실험 종료일까지도 그 증식은 정체되는 양상을 보였다. 그러나, T. japonicus의 증식은 T. japonirus를 단독 배양한 경우에 비교하여 B. rotundiformis와 혼합 배양하여도 그 증식에는 큰 차이가 없었다. 또한, B. rotundiformis와 T japonicu의 혼합 배양에서 두 종간의 피포식 관계나 먹이 경쟁 관계와 같은 직접적인 상호 작용은 관찰할 수 없었다. 이 연구의 결과, 로티퍼 B. rotundiformis의 대량 배양조에서 혼재 생물로서 자주 관찰되는 코페포다 T. japonicus는 B. rotundiformis의 증식 저해요인으로서 작용하고 있음을 알 수 있었다.

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한국산 돌나물속 Telephium 절 식물의 분류학적 연구 (A Taxonomic Study on Sedum Section Telephium in Korea)

  • 정영호
    • Journal of Plant Biology
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    • 제33권1호
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    • pp.1-24
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    • 1990
  • This study was attempted to clarify the taxonomic problems that have been raised up to now in the study o section Telephium, genus, Sedum: the delimitation of taxa belong to Telephium group, their relationship, and nomenclatural confusion and the rank of Telephium group. Specimens were collected at 19 sites in Korea. The habitat, distribution, external morphological characters of habit, leaf shape, phyllotaxy, inflorescence, color of floral organs, floral formula, carpel, capsule, and micro-characters of pollen, seed coat, stigma and anther with SEM were examined. Through this study, the following results were obtained. The delimitation of taxa that belong to section Telephium in Korea and their relationship Nomenclatural errors were corrected. In S. rotundifolium Lee, species epithet“rotundifolium”is later homonym of s. rothundifolium Lamarck, so corrected to S. duckbongii Chung and Kim by article 64 of ICBN. Phyllotaxy was elucidated to meaningless, character in sect. Telephium. S. taquetii was a separate species from S. viridescens and not of its synonym. S. alboroseum Baker in Korea was S. erythrostictum sensu Master not of S. erythrostictum Miquel. By this study , taxa of sect. Telephium in Korea were arranged to 7 species. Considering interspecific relationship of sect. Telephium, S. duckbongii and S. alboroseum are considered to be more advanced forms and S. viridescens, S. taquetii, and S. viviparum to be more primitive forms among 7 species.

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경남지역 둥굴레속의 Cluster 분석에 의한 종간 유연관계 (Interspecific Relationship of Polygonatum Species Collected from Gyeongnam Area Using Cluster Analysis)

  • 심재석;박정민;전병삼;강진호
    • 한국약용작물학회지
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    • 제13권1호
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    • pp.30-34
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    • 2005
  • 경남지역 자생 둥글레속 수집종의 유연관계를 규명하여 품종육성을 위한 기초자료로 할용하고자 47개 수집종의 12개 형질을 대상으로 주성분 분석 및 average linkage cluster 방법으로 군을 분류한 결과는 다음과 같다. 1. 주성분 분석에서 줄기형태, 줄기색깔, 엽색, 포의 형태, 화색, 화형 등 12개 주요 형질의 주성분의 고유치에 기여율은 제3주성분까지 분석하여도 전체의 79%정도 설명할 수 있어서 47개 수집종의 분류가 가능함을 알 수 있었다. 2. 47개 수집종의 12개 주요형질을 대상으로 average linkage cluster 방법에 의한 분석치으 거리 0.7을 기준으로 군을 분류한 결과 5개군으로 분류되었다. I군은 둥굴레, II군은 용둥글레, III군은 산둥굴레 및 퉁둥굴레 특성의 중간형태를 각각 지닌 p28과 p29였으며, 제V군은 죽대로 분류되었다. 3. 경남일대 둥굴레의 특성조사와 수집종 분류결과 둥굴레와 죽대는 종간 변이가 거의 없었던 반면, 용둥굴레, 퉁둥굴레와 산둥굴레는 다양한 변이종이 분포하고 있는 것으로 나타났다.

濟州 地域에서 개망초의 發芽 習性 및 耐陰性과 群落特性 (Germanition, Shade Toarance and Community Characteristics on $\emph{Erigeron annuus}$ L.in Cheju)

  • Lee, Ho-Joon;Kim, Tae-Sung;Byun, Doo-Weon
    • The Korean Journal of Ecology
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    • 제15권2호
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    • pp.103-115
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    • 1992
  • This dissertation, which has been prepared in the region of cheju(33o31'N, 126o32'E) from may,1987 to appril, 1989, is to elucidate the dominant characteristics of early stages in secondary succession, by examining the life history of erigeron annuus for the seeding depth of a seed was dependent upon the degree of shading. That is, on the area of ocm,the more tense the shading was, the more germination percentage was increased, while, in the case of 1.5cm, it was somewhat increased, when shading bwcame ess and less tense. The rosette of e. annuus, through its relative light intensity was decreased by shading, up to 5% of natural light, showed some strong shade tolerance, which had no difficulty in matter production. The species diversity of mixed-species community was the highest in the middle of April, the lowest in the last of may. The dominance index was the highest in the late may, the lowest in the middle of April. Toward the end of may, the occupation rate of dominance species was the highest. There was a neutral relationship between intraspecific and interspecific, owing to the differentiation of its niche, temporally and spatially. Productive structure of the community revealed a narrow leaf type which was concentratively distributed in the mid part of community height. The relative light intensity of community ground surface was 6.1%, the leaf inclination 60o,the extinction coefficient(k) 0.4, biomass of community 1,045.6 g.d.w./m2,T/Rratio 9.3, C/Frate 7.0 and sumgermanition, shade toarance and community characteristics on erigeron annuus l.in chejumed leaf area index 3.88. through the various life cycles o e.annuus, we can say that it decreases mortality of seeding caused by some stress and disturbance, for germination lasts for a long time at any opportunity available, and it promotes population growth. The strong shade tolerance of a rosette and the variableness of a life from differentiate the niche between intraspecific and interspecific within the community, and avoid the direct competition between them, thus poromoting community growth.

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Mitochondrial Cytochrome b gene의 분석에 의한 한국산 미꾸리과 어류(Cobitidae)의 계통 (Molecular Phylogeny of Korean Loaches Inferred from Mitochondrial DNA Cytochrome b Sequences)

  • 김소영;김익수;장광엽;장미희
    • 한국어류학회지
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    • 제12권4호
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    • pp.223-229
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    • 2000
  • 한국산 미꾸리과 어류의 계통유전학적 관계를 고찰하고자 8종의 mitochondrial cytochrome b의 유전자 서열을 비교한 결과 대부분 이전의 형태학적 연구의 결과와 일치하였다. 그러나 종개속 Orthrias과 쌀미꾸리속 Lefua의 분류학적 위치는 미꾸리과 Cobitidae와 paraphyletic group으로 나타났으며 이 두 속의 sequence divergence는 0.184~0.272으로 나타나 미꾸리과와 잉어과 사이의 divergence와 유사하였다. 한편 참종개속 Iksookimia 2종과 북방종개 Cobitis melanoleuca는 각각 다르게 분화한 결과를 보여 주었으며 또한 중국산 미꾸리와 한국산 영덕 미꾸리의 sequence divergence는 0.099로 종간의 divergence를 보여주어 주목되었다. 미꾸리과 어류 가운데 참종개속의 일부 어류는 분류학적 위치로 보아 이들의 기원이 미꾸리과의 속간 잡종기원으로 생각된다.

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Evaluation of Growth and Wood Traits in E. camaldulensis and Interspecific Eucalypt Hybrid Clones Raised at Three Diverse Sites in Southern India

  • Rathinam Kamalakannan;Suraj Poreyana Ganapathy;Shri Ram Shukla;Mohan Varghese;Chandramana Easwaran Namboothiri Jayasree
    • Journal of Forest and Environmental Science
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    • 제39권1호
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    • pp.27-39
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    • 2023
  • Twenty-five Eucalyptus clones (14 E. camaldulensis - EC and 11 interspecific eucalypt hybrid clones - EH) grown in three contrasting sites were evaluated for the growth and few wood traits at 4 years of age. The stability, genotype-site interaction and suitability of these clones for pulp and solid wood industry sectors were studied. Growth of eucalypt clones was significantly higher at site 1 with higher rainfall, but wood density did not differ significantly from lower rainfall sites. Kraft pulp yield (KPY) decreased from sites 1 to 3 based on moisture availability, but not between two groups of clones. Volumetric shrinkage (VS) was significantly higher in EC clones at site 3 with lowest rainfall, but there was no specific trend at other two sites with maximum (site 1) and intermediate (site 2) rainfall. The mechanical traits modulus of rupture (MOR) and modulus of elasticity (MOE) were at par in sites 1 and 2, but significantly lower at the driest site 3. The growth rate had a significant positive correlation with KPY, MOR and MOE and a negative correlation with VS, but no significant impact on wood density in both groups of clones. Genotype×environment interaction (G×E) was evident in most traits due to the difference in response of clones to moisture availability. Since wood density was negatively correlated to KPY, it has to be kept at an optimum level for the profitability of pulp industry. There was no significant difference between EC and EH clones for most traits except VS at site 3. Stability of clones varied across sites in different traits, and hence clones may be selected for deployment at each site by screening for growth, followed by wood density, considering the relationship of growth and density with other traits required by pulp and solid wood industry sectors.

동위효소 분석에 의한 느타리속의 종간 유연관계 (Interspecific Relationships within the Fungal Genus Pleurotus by Isozyme Analysis)

  • 이희경;유영복;차동열;민경희
    • 한국균학회지
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    • 제26권2호통권85호
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    • pp.163-172
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    • 1998
  • 국내외에서 수집된 Pleurotus속 13종 32균주와 종(species)이 밝혀지지 않은 4균주 등 모두 36균주의 동위효소 분석에 의한 종간 유연관계를 파악하기 위하여 본 실험을 수행하였다. Pleurotus속 균주들의 균사체에서 수용성 단백질을 추출하여 등전점 전기영동 isozyme polymorphic banding pattern법으로 여섯 종류의 동위효소 즉 esterase, glucosephosphate isomerase, leucine aminopeptidase, malate dehdrogenase, peroxidase, phosphoglucomutase를 분석하여 총 166개 밴드를 확인하였고 36개 느타리 균주의 종간 다형성을 관찰하였다. Esterase가 종간에 가장 다양한 밴드 pattern을 나타내어 종의 구별에 아주 유용한 동위효소라 생각되며, 다른 동위효소에서도 종간 다형성을 관찰할 수 있었다. 동위효소 분석 결과 유연관계가 가장 가까운 종은 P. florida와 P. sajor-caju로 유사도가 약 89%였으며, 연구에 이용된 총 13종간의 유사도는 약 77%였다. 분류상 논란이 많이 되는 P. ostreatus는 P. pulmonarius 종과 확실히 구별되었으며 P. florida도 독립된 종으로 판명되었다. 그러나 P. sapidus와 P. spodoleucus 균주들은 확실한 종의 판명이 어려웠다. Isozyme polymorphic banding pattern 결과와 현미경적인 형태 분류에 의한 종 판명 결과가 몇 종을 제의하고는 일치하였다. 동위효소를 이용한 분류는 매우 유용하다고 판단되었다.

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왕대속 3종의 지역개체군간 동위효소변이 (Isozymic Variation of Local Populations in the 3 Species of Phyllostachys)

  • 김창호;하미영;이호준
    • The Korean Journal of Ecology
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    • 제21권3호
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    • pp.301-307
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    • 1998
  • Intraspecific and interspecific isozyme variations and their relationship of 16 local populations in 3 species of Phyllostachys, that is, P. bambusoides, P. nigra var. henonis and P. pubescens were investigated by multi-variate analysis. Leaf isozymes of Phyllostachys such as 6-PGD (6-phosphogluconate dehydrogenase), MDH (malate dehydrogenase), PGI (phosphoglucoisomerase), PRX (peroxidase), PGM (phosphoglutamase), IDH (isocitrate dehydrogenase) showed electrophoretic variations in the number of zymotypes (7, 6, 6, 9, 3 and 5, respectively). In the cluster analysis on the isozymic characteristics, 16 populations were classified into 3 species at the euclid genetic distance of 2.041. P. nigra var. henonis and P. bambusoides were clustered first at 2.813 and then P. pubescens at 3.001. So far, 3 local types of intraspecific ariation were found in P. nigra var. henonis and P. bambusoides.

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