• International Journal of Industrial Entomology and Biomaterials
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• 제18권1호
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• pp.41-48
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• 2009

The large bumblebee, Bombus terrestris, indigenous to Europe and used extensively for high-value crop pollination, has been artificially introduced in several parts of the world. Here we show the interspecific hybridization between bumblebee species, B. terrestris and B. ignitus, under laboratory conditions. The mating and oviposition percentages of the interspecific hybridization of a B. terrestris queen with a B. ignitus male were higher than those of the intraspecific mating of B. ignitus. Furthermore, the competitive copulation experiment indicated that the mating of B. ignitus males with B. terrestris queens was 1.8-fold more frequent than with B. ignitus queens. The interspecific hybridization of a B. ignitus queen with a B. terrestris male produced either B. ignitus workers or the B. ignitus male phenotype, and the hybridization of a B. terrestris queen with a B. ignitus male produced B. terrestris males. Genetic tests using a portion of the mitochondrial COI gene for the parent and hybrid phenotypes indicated that mitochondrial DNA in the interspecific hybridization was maternally inherited. Our results indicated that interspecific hybridization occurred between B. ignitus and B. terrestris, which suggests that the hybridization will have a negative impact of competition and genetic pollution of native bumblebees.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.167-174
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• 2011

The large bumblebee $Bombus$ $terrestris$, indigenous to Europe and adjacent areas and used extensively for high-value crop pollination, has been artificially introduced to several parts of the world. Here we show the occurrence of interspecific hybridization between the bumblebee species $B.$ $hypocrita$ $sapporoensia$ and $B.$ $terrestris$ under laboratory conditions. The mating and oviposition percentages resulting from the interspecific hybridization of a $B.$ $terrestris$ queen with a $B.$ $h.$ $sapporoensis$ male were higher than those resulting from the intraspecific mating of $B.$ $h.$ $sapporoensis$. Furthermore, a competitive copulation experiment indicated that the mating of $B.$ $h.$ $sapporoensis$ males with $B.$ $terrestris$ queens was 1.2-fold more frequent than the mating of these males with $B.$ $h.$ $sapporoensis$ queens. The interspecific hybridization of a $B.$ $terrestris$ queen with a $B.$ $h.$ $sapporoensis$ male produced either $B.$ $terrestris$ workers or the $B.$ $terrestris$ male phenotype, and the hybridization of a $B.$ $h.$ $sapporoensis$ queen with a $B.$ $terrestris$ male produced $B.$ $h.$ $sapporoensis$ males. Our results indicated that interspecific hybridization occurred between $B.$ $h.$ $sapporoensis$ and $B.$ $terrestris$. These results suggest that such hybridization will have a negative competitive impact and will cause genetic contamination of native bumblebees.

• Plant Resources
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• 제5권1호
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• pp.1-6
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• 2002

Genetic improvement of the cultivated soybean [Glycine max (L.) Merr] may be possible through hybridization with its wild progenitor, G. soja Sieb. & Zucc. Interspecific cross between G. max (Hwangkeumkong) and G. soja (IT.182932) was made in the summer of 1997. In F$_2$ the percentage of plant height, nodes per plant, and pods per plant were high but gradually reduced from F$_2$ to F$_4$. In contrast pod length, seeds per pod, and 100-seeds weight were increased gradually through generations advanced. Wild variation as evident in F$_2$ in plant height, number of branches, pods per plant, and 100-seeds weight. Twenty six percent of the F$_2$, 44 % of the F$_3$ and 60% of the F$_4$ segregants showed more G. max traits. The combination of useful traits from both species is possible through interspecific hybridization. The characters that could be transferred from wild species to cultivated species are more pod number, better capacity, and resistance to disease and insects. The interspecific derivatives offer scope for selection for high grain yield. Therefore, introducing genes from G. soja to G. max could be contribute to greater genetic diversity of future cultivars. And semicultivated soybean had some desired characteristics including tolerance to adverse environments and multi-seed characters. It means the infusing of semicultivated germplasm to the cultivated soybean could increase number of seeds and pods per plant significantly, and consequently could enhance selecting potential on yield.

• 한국작물학회지
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• 제41권5호
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• pp.608-618
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• 1996

Glycine속의 야생 다년생종인 G. tomentella가 가지고 있는 내병성, 고단백 및 다년생 유전자등 유용형질들을 재배콩(G. max)에 도입하기 위한 종간교잡시 염색체수가 다른 4개의 G. tomentella (2n=38, 40, 78, 80) 계통들과 재배콩과의 교잡친화성 검정, 정역간 교잡효과, 교잡엽의 발육비교, 종간 교배적기 등에 대해 시험한 결과를 요약하면 다음과 같다. 1. G. tomentella의 염색체수가 2n=38, 40, 78, 80인 4개 계통과 재배콩인 울산콩, 뱀콩, 단엽콩과의 종간 교잡에서 G. tomentella의 염색체수가 2n=38인 계통과 교배된 것이 교배율 및 교배후 경과일수에 따른 협생존률이 가장 양호하여 2n=38계통이 재배콩과의 교잡친화성이 가장 높은 것으로 나타났다. 2. G. max(cv. Baemkong)와 G. tomentella(2n=38)와 종간교잡에서 정역교잡 효과는 G. max를 모본으로 했을 때가 G. tomentella를모본으로 했을 때보다 교배율이 더 높았고, 엽생존기간도 더 길어 정역교잡의 효과가 인정되었다. 3. G. max(cv. Baemkong)와 G. tomentella(2n=38)와의 종간교잡에서 교잡협의 발육상태를 자가수정된 모본의 협과 비교했을 때 G. max를 모본으로 한 것이 G. tomentella를 모본으로 한 것보다 협당 배주수의 감소율이 적었으나, 교배후 경과일수에 따른 협의 장, 폭 및 무게는 G. max를 모본으로 한 경우가 생육이 저조하였다. 4. 자연조건에서 G. max와 G. tomentella(2n=38)와의 종간 교잡을 할 경우 6월 이나 7월에 하는 것보다 대체로 온도와 습도가 높고 단일조건인 8월 중하구에 하는 것이 교배율이 높았다.

• 한국연초학회지
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• 제7권1호
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• pp.93-96
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• 1985

This study was conducted to establish the maternal haploid method for the practical breeding of M tabacum using the interspeciflc hybridization between M tabacum and N. aflicana. The frequency of surviving seedling per seed capsule of interspecific hybridization was 4.15. Among them, the frequencies of maternal haploid and hybrid were 1.20 and 2.95, respectively. The chromosome numbers of n=24 for maternal haploid and 2n=47 for hybrid were identified in surviving seedling from interspecific hybridiztion. Except the chromosome number, distinguishable morphological differences of material haploid from hybrid were observed at seedling stage.

• 한국수산과학회지
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• 제54권2호
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• pp.170-179
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• 2021

This study was conducted to identify taxonomic differences in the characteristics of Acheilognathus majusculus and A. yamatsutae during their initial life history via an interspecific hybridization experiment. Hatching time required 36 h for MY and 49 h for YM at 21.5℃, showing a significant difference of 13 h between the hybrids. The hatching rates of the cross-bred eggs were 30% for cross MY (A. majusculus♀×A. yamatsutae♂) and 40% for cross YM (A. yamatsutae♀×A. majusculus♂). The hatching larvae size was total length 3.13-3.43 mm in MY and total length 3.89-4.22 mm in YM, which was larger in YM. The hybridization test between A. yamatsutae and A. majusculus that live in the same water stream confirmed that no interspecific reproductive isolation occurred.

• Fisheries and Aquatic Sciences
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• 제21권5호
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• pp.11.1-11.8
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• 2018

Interspecific hybridization experiments were conducted between the common seahorse Hippocampus kuda (male) and the slender seahorse H. reidi (female) during artificial rearing to develop a new aquarium fish with unique polyandrous mating. Molecular analysis via mitochondrial DNA (mtDNA) cytochrome b and nuclear DNA (ncDNA) ribosomal protein S7 gene supported the hybridization between the two species, and the hybrid also showed morphological characteristics of both species. Juveniles of H. kuda have dense melanophores on the whole body or only on the trunk and tail, whereas juveniles of H. reidi have thin melanophores on the whole body or present in stripes only along their prominent trunk and tail rings. However, all the hybrid juveniles had dense melanophores only on the tail, with the striped trunk rings, thus showing an intermediate pattern, and these patterns were limited to the fairly early stage of development (1-10 days old). In contrast, the two eye spines in the hybrid were apparent after 9 days old, which were not inherited from H. kuda (one eye spine), but from H. reidi (two eye spines). According to LOESS (local regression) analysis, the growth rate increased between 20 and 25 days, and the hybrids grew faster than H. kuda when they entered the explosive second phase of growth between 25 and 45 days for all the seahorses. This study highlights the hybridization between H. kuda and H. reidi may contribute to the improved taxonomic information of young seahorses.

• 한국환경생태학회지
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• 제29권2호
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• pp.145-161
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• 2015

This research was conducted in order to understand the hybridization between Quercus aliena Blume and Q. serrata Murray in Korea which show wide range of morphological variations within species and interspecific variations of diverse overlapping characteristics caused by hybridization. Morphological analysis (principal components analysis; PCA) of 116 individuals representing two species and their intermediates were performed. As a result, two species were clearly distinguished in terms of morphology, and intermediate morpho-types assumed to be hybrids between the two species were mostly located in the middle of each parent species in the plot of the principal components analysis. There was a clear distinction between two species in trichome distribution pattern which is an important diagnostic character in taxonomy of genus Quercus, whereas intermediate morpho-types showed intermediate state between two species' trichome distributions. Forty-two individuals representing two species and their intermediates were examined for leaf flavonoid constituents. Twenty-three flavonoid compounds were isolated and identified: They were glycosylated derivatives of flavonols, kaempferol, quercetin, isorhamnetin and myricetin. The flavonoid constituents of Q. aliena were five glycosylated derivatives: kaempferol 3-O-galactoside, kaempferol 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-glucoside, and Isorhamnetin 3-O-glucoside. The flavonoid constituents of Q. serrata had 20 diverse flavonol compounds including five flavonoid compounds found in Q. aliena. It was found that there is a clear difference in flavonoid constituents of Q. aliena and Q. serrata. Flavonoid chemistry is very useful in recognizing each species and putative hybrids. The flavonoid constituents of intermediates were a mixture of the two species' constituents and they generally showed similar characteristics to morpho-types. The hybrids between Q. aliena and Q. serrata showed morphologically and chemically diverse characteristics and it is assumed that there are frequent interspecific hybridization and introgression.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.11-11
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• 2002

• Journal of Plant Biology
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• 제15권3호
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• pp.14-18
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• 1972

This paper includes a review on recent development on protoplast culture, regeneraton of plant from protoplast, and fusion of isolated protoplasts, and also describes the possibility of obtaining interspecific hybrid plants through asexual fusion of protoplasts of cells from distantly related plants which are not crossed by the ordinary sexual method.