• Journal of Microbiology and Biotechnology
• /
• v.23 no.8
• /
• pp.1154-1158
• /
• 2013

Ganciclovir resistance of human cytomegalovirus is associated with mutations in the viral UL97 gene and poses severe problems for immunocompromised patients. In this study, PCR-based restriction fragment length polymorphism and sequencing analyses detected the UL97 D605E mutation in all five clinical isolates from patients with ganciclovir-resistant human cytomegalovirus infection during prolonged ganciclovir therapy, whereas the M460V mutation was only present in 1 of 5 isolates. On the other hand, the detection rates of the D605E mutation in the stored available DNA samples from the donor and allogeneic stem cell transplantation recipients were 66.7% and 93.7%, respectively, suggesting that the presence of D605E mutation was not associated with the ganciclovir exposure. Although the D605E mutation may not be related to ganciclovir resistance, we suggest that this mutation could be an important molecular marker of human cytomegalovirus evolution in East Asian countries. Moreover, the restriction fragment length polymorphism method using the restriction enzyme HaeIII, which is generally used to detect the UL97 A591V mutation, could also detect the D605E mutation and may therefore be a useful tool for future research on the investigation of UL97 gene mutations.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.54 no.4
• /
• pp.137-145
• /
• 2012

Sand is one of the essential materials used for social infrastructure construction such as embankment, landfill and backfill. It was known that mechanical properties and shear strength of sand are closely related to relative density. Therefore it is very important to determine accurate relative density. In this study, Portable Cone Penetration Tester (PCPT) was used to estimate the relative density and the internal friction angle of sand. PCPT cone-tip resistance ($q_c$) was measured changing the relative density of the two soil samples.Standard sand (JMJ) and Busan sand (BS). Also, a direct shear test was performed to investigate relationship between relative density and internal friction angle. The size and shape of soil particles were confirmed by using Scanning Electron Microscope (SEM). As a result, the log value of $q_c$ was linearly correlated with relative density and internal friction angle. In particular, the internal friction angle of BS sample was greater than that of JMJ, which was due to difference of the shape and mean size of particles. This result shows that it is important to determine the shape and size of particles as well as relative density to define mechanical property of sand. Through this study, it can be more effectively and conveniently to investigate relative density and shear strength of sand by using PCPT in situ.

• The Journal of Internal Korean Medicine
• /
• v.21 no.4
• /
• pp.555-563
• /
• 2000

Objective : In order to know the antibacterial effects of Kyungok-go against Mycobacteria tuberculosis, Methods : In this study, I inverstigated these effects in terms of combination of other antibiotics with and without Kyungok-go on several different media conditions. Results: On Ogawa medium, Kyungok-go of the $10{\mu}/ml$ concentration showed the anti-Mycobacteria tuberculosis activity against antibiotic drug-sensitive strain. On Middle-blue medium, Kyungok-go of the $10{\mu}/ml$ concentration showed the anti-Mycobacteria tuberculosis activity against antibiotic drug-sensitive strain. Kyungok-go showed the anti mycobacteria tuberculosis activity with the meaningful result above a certain concentration. The resistance against M, tuberculosis as the concentration of Kyungok-go was decreased significantlly on the high concentration($500{\mu}/ml$) When rifampicin and Kyungok-go were used together, the resistance was decreased with the statistical significance as to the persistant antibacterial effect against M. tuberculosis, When ciprofloxacin and Kyungok-go were used together, the resistance was decreased with the statistical significance as to the persistant antibacterial effect against M. tuberculosis, The combination of treatment, Kyungok-go with both rifampicin and ciprofloxacin, showed much better antibacterial effect against M, tuberculosis than antibiotics alone. Conclusions : This study shows that Kyungok-go has antibacterial effect against M. tuberculosis and in the combination of treatment, Kyungok-go with antibiotics, showed much better antibacterial effect against M. tuberculosis than antibiotics alone,.

• Journal of the Semiconductor & Display Technology
• /
• v.21 no.3
• /
• pp.80-86
• /
• 2022

In this paper, the optimal design and circuit simulation verification results of an LLC resonant half-bridge dc-dc converter using a steady-state model with internal loss resistance are reported. Above all, the input/output voltage gain and frequency characteristic equations in the steady-state were derived by reflecting the internal loss resistance in the equivalent circuit. Based on the results, an LLC resonant half-bridge dc-dc converter with an input voltage of 360-420V, an output voltage of 54V, and a maximum power of 3kW was designed, and to verify the design, the PSIM circuit simulation was executed to compare and analyze the result. In particular, the operating range of the converter could be drawn from the frequency characteristic graph of the voltage gain, and when the converter was operated under light and maximum load conditions, it was confirmed that similar results were obtained by comparing simulation results and calculation results in the switching frequency characteristic graph. In addition, the change of the switching frequency with respect to the load current at each input voltage was compared with the calculated value and the simulation result. As a result, it was possible to confirm the usefulness of the analysis result reflecting the internal loss resistance proposed in this paper and the process of the optimal design.

• The Journal of Internal Korean Medicine
• /
• v.30 no.2
• /
• pp.257-269
• /
• 2009

Objective : Obesity is an important cause of diabetes, and lipotoxicity causes insulin resistance. Recently a lot of research is being done on PPAR-${\alpha}$. TNF-${\alpha}$. adiponectin, and leptin, which are important obesity related factors. In this study, we investigated the effects of Supungsunki-hwan on high fat. high carbohydrate diet-induced obese type 2 diabetic mouse models. Methods: Diabetes was induced in ICR male mouse (30${\pm}$5g) with Surwit's high fat, high sucrose diet. Mice were divided into 4 groups(n=10) of Normal. Control. Supungsunkj-hwan group. and acarbose group. The Supungsunki-hwsn group was given 10% Supungsunkj-hwan in their diet. and the acarbose group was given 0.5% acarbose in their diet. After 6 weeks. body weight. food intake, FBS and OGTT. lipid profile and liver enzymes, epididymal fat weight, and gene expression of leptin, adiponectin, TNF-${\alpha}$ and PPAR-${\alpha}$ were measured. Leptin. adiponectin. tumor necrosis factor(TNF)-${\alpha}$ and peroxisome proliferator-activated receptor (PPAR)-${\alpha}$ were evaluated by reverse transcription-polymerase chain reaction. Results : Supungsunkj-hwan increased the gene expression of PPAR-${\alpha}$, which reduces lipotoxicity and insulin resistance. Supungsunkj-hwan also significantly reduced triglyceride. AST. ALT serum levels. and 1 hour oral glucose tolerance levels. Conclusion : These results show that Supungsunkj-hwan improves insulin resistance in the liver and muscles, by reducing triglyceride levels and lipotoxicity through increased PPAR-${\alpha}$ gene expression. This is supported by the fact that Supungsunkj-hwan significantly reduces 1 hour oral glucose tolerance levels. Therefore we suggest that Supungsunkj-hwan would be an effective treatment for obese type 2 diabetic patients.

• Journal of Ocean Engineering and Technology
• /
• v.16 no.5
• /
• pp.34-40
• /
• 2002

A numerical model has been developed for the permeable coastal structures to simulate hydraulic characteristics on the permeable slopes, which interact with internal four field the structures. The model includes hydraulics in the porous medium. Numerical model was calibrated using hydraulic model experiments performed in 2-D wave flume in the Institute of Ocean Hydraulics in PKNU. Better aggrements were obtained with the model which employed inertia resistance term than with the conventional model, PBREAK.

• The Transactions of the Korean Institute of Electrical Engineers P
• /
• v.65 no.3
• /
• pp.210-215
• /
• 2016

The transient characteristics of the Mg/Air fuel cell were ascribed to the load current, electrolyte concentrations, air electrode area and electrode distance. It was found that transient phenomena occurred in the load current, which is due to activate of the oxidation and reduction reaction process. The transient time increase with the load current increase. The transient characteristics were investigated with regard to internal resistance. The maximum power output analysis was employed in order to explain the delayed action under various experimental conditions. The internal resistances had a significant effect on the transient characteristics. The transient curves thus obtained were in almost agreement with internal resistance characteristics.

• Tuberculosis and Respiratory Diseases
• /
• v.73 no.1
• /
• pp.32-37
• /
• 2012

Background: This study evaluates the bacterial pathogens of Ventilator-associated pneumonia (VAP) in a tertiary referral hospital. Methods: A total of 109 bacterial pathogens from 91 adult patients with VAP, who were admitted to the medical intensive care unit from January 2008 to December 2009, were examined. Clinical characteristics, bacterial pathogens, and resistance profiles were analyzed. Results: Staphylococcus aureus (44%) was the most frequently isolated. Acinetobacter baumanii (30%), Pseudomonas aeruginosa (12%), Stenotrophomonas maltophilia (7%), Klebsiella pneumoniae (6%), and Serratia marcescens (2%) were isolated from the transtracheal aspirates or bronchoalveolar lavage in patients with VAP. There was no significant difference of bacterial pathogens between early and late onset VAP. All isolated S. aureus were methicillin resistant S. aureus; the imipenem resistance rate of A. baumanii was 69%. Conclusion: The two most frequent pathogens of VAP were S. aureus and A. baumanii. There were no pathogenic differences between early and late onset VAP.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.39 no.3
• /
• pp.369-380
• /
• 2019

When a extreme loading such as blast is applied to prestressed concrete (PSC) structures and infrastructures for an instantaneous time, serious property damages and human casualties occur. However, a existing design procedure for PSC structures such as prestressed containment vessel (PCCV) and gas storage tank do not consider a protective design for extreme internal blast scenario. Particularly, an internal blast is much more dangerous than that of external blast. Therefore, verification of the internal blast loading is required. In this paper, the internal blast resistance capacity of PSC member is evaluated by performing internal blast tests on RC and bi-directional PSC scaled down specimens. The applied internal blast loads were 22.68, 27.22, and 31.75 kg (50, 60, and 70 lbs) ANFO explosive charge at 1,000 mm standoff distance. The data acquisitions include blast pressure, deflection, strain, crack patterns, and prestressing force. The test results showed that it is possible to predict the damage area to the structure when internal blast loading occurs in PCCV structures.

• Journal of Korean Society of Water and Wastewater
• /
• v.32 no.2
• /
• pp.77-87
• /
• 2018

In this study, the effects of operating conditions on the formation of reversible and irreversible fouling were investigated in the filtration using ceramic membrane for water treatment process. The effect of coagulation pretreatment on fouling formation was also evaluated by comparing the performance of membrane filtration both with and without addition of coagulant. A resistance-in-series-model was applied for the analysis of membrane fouling. Total resistance (RT) and internal fouling resistance (Rf) increased in the membrane filtration process without coagulation as membrane flux and feed water concentrations increased. Internal fouling resistance, which was not recovered by physical cleaning, was more than 70% of the total resistance at the range of the membrane flux more than $5m^3/m^2{\cdot}day$. In the combined process with coagulation, the cake layer resistance (Rc) increased to about 30-80% of total resistance. As the cake layer formed by coagulation floc was easily removed by physical cleaning, the recovery rate by physical cleaning was 54~90%. It was confirmed from the results that the combined process was more efficient to recover the filtration performance by physical cleaning due to higher formation ratio of reversible fouling, resulted in the mitigation of the frequency of chemical cleaning.