• Molecules and Cells
• /
• 제26권6호
• /
• pp.554-557
• /
• 2008

Double-stranded RNA (dsRNA) induces gene silencing in a sequence-specific manner by a process known as RNA interference (RNAi). The RNA-induced silencing complex (RISC) is a multi-subunit ribonucleoprotein complex that plays a key role in RNAi. VIG (Vasa intronic gene) has been identified as a component of Drosophila RISC; however, the role VIG plays in regulating RNAi is poorly understood. Here, we examined the spatial and temporal expression patterns of VIG-1, the C. elegans ortholog of Drosophila VIG, using a vig-1::gfp fusion construct. This construct contains the 908-bp region immediately upstream of vig-1 gene translation initiation site. Analysis by confocal microscopy demonstrated GFP-VIG-1 expression in a number of tissues including the pharynx, body wall muscle, hypodermis, intestine, reproductive system, and nervous system at the larval and adult stages. Furthermore, western blot analysis showed that VIG-1 is present in each developmental stage examined. To investigate regulatory sequences for vig-1 gene expression, we generated constructs containing deletions in the upstream region. It was determined that the GFP expression pattern of a deletion construct (${\Delta}-908$ to -597) was generally similar to that of the non-deletion construct. In contrast, removal of a larger segment (${\Delta}-908$ to -191) resulted in the loss of GFP expression in most cell types. Collectively, these results indicate that the 406-bp upstream region (-596 to -191) contains essential regulatory sequences required for VIG-1 expression.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 1997년도 춘계학술대회 논문집
• /
• pp.61-67
• /
• 1997

The electronic speckle pattern interferometer (ESPI) has been applied to many technical problems such as deformation and displacement measurement, strain visualization and surface roughness monitoring. In this study, we used an ESPI system based on the dual beam speckle interferometer method in order to measure in-plane displacement and vibration mode using the ESIP technique. This research was carried out for the purpose of applying the vibration analysis method employing Electro-Optic holographic interference technique to the vibration analysis of uniform rectangular cantilevers plate(SS400,STS304) with cantilevers span to breadth ratio of 150 by 75. And thickness of 1mm and 0.8mm respectively. We improved the ESPI technique in order to obtain the distribution of displacement component resolved in one direction through a CCD camera combined with an image processing system. To certify and to assess the accuracy in measuring by this ESPI, the results obtained with the speckle method and vibration mode analysis are to be compared with those results by Warbuton's Theoretical expression and vibration made in FEM analysis.

• 한국정밀공학회지
• /
• 제11권5호
• /
• pp.161-170
• /
• 1994

This paper presents the performance and problems in analysis method and testing system of Electronic Speckle Pattern Interfermetry(ESPI) method, in measuring two-dimensional in- plane displacement. The analysis result of measurement by ESPI is quite comparable to that of measurement by strain gauge method. This implieds that the method of ESPI is a very effective tool in non-contact two-dimensional in-plane strain analysis. But there is a controversial point, measurement error. This error is discussed to be affected not by ESPI method itself, but by its analysis scheme of the interference fringe, where the first-order interpolation has been applied to the points of strain measured. Further development of advanced first-order interpolation method is being undertaken for the more precise in-plane strain measurement.

• 한국산업융합학회 논문집
• /
• 제5권3호
• /
• pp.187-192
• /
• 2002

In-plane ESPI(Electronic Speckle Patten Interferometry)was devised to measure in~plane defamation and rotation of a specimen with laser in this study. The conventional measuring methods of surface deformations such as the strain gauge have many demerits because they are contact and point-to-point measuring ones. But that ESPI is noncontact, nondestructive and whole field measuring method can overcome previous disadvantages. We used ESPI which is sensitive to in-plane displacement for measuring in-plane deformations of a disk. First of all, the system calibration was done due to an in-plane rotation before getting deformations of a disk. Finally we showed good agreement between theexperiment results and those of the FEA(Finit Element Analysis).

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1987년도 전기.전자공학 학술대회 논문집(II)
• /
• pp.1284-1287
• /
• 1987

A method of quantitative electrocardiogram analysis, based on concepts drawn from syntactic pattern recognition theories, is described. The algorithm can be used for removing the Interference noises and base line drift as a filter function, and for reducing the number of points representing the digitized ECG waveform. The Parsing is performed with simple finite state automata inferred by experiments and suitable to be updated during experiment execution. Two parameters are utilized for defining the noise and these make the algorithm flexible. The examples for testing the algorithm is real ECG waveforms with noise. Some experimental results lire presented.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
• /
• pp.939-942
• /
• 2008

Moir$\acute{e}$s are a natural interference phenomenon which occurs whenever a transparent regular pattern plate is overlapped on another regular pattern plate. In the contact-type 3 dimensional imaging systems, the moires are inherent because an image display panel is seen through a viewing zone forming optical plate. The mathematical analysis of moires in the systems shows that they can be minimized by the proper selection of overlapping angles between them. The angle is different for pixels with different aspect ratios.

• 한국분무공학회지
• /
• 제25권2호
• /
• pp.51-59
• /
• 2020

The purpose of this study is to propose and compare methods for measuring individual spray cone angles using spray cross-section images. In direct injection gasoline engines, it was believed that the distribution of air-fuel mixture in the combustion chamber directly affected combustion performance and emission formation. However, since gasoline direct injection (GDI) injectors have a small injection angle, interference between individual spray plumes occurs. Therefore, GDI injectors have only measured the spray angle of the entire spray. To overcome these limitations, three methods of indirectly measuring the spray cone angles of individual spray plume were presented and compared by forming sheet beams using Nd:YAG laser and acquiring spray cross-section images. Each method currently has advantages and disadvantages, and research to apply the method suitable for various GDI injectors needs to be continued.

• 한국정밀공학회지
• /
• 제12권10호
• /
• pp.40-49
• /
• 1995

This paper presents the performance and problems in analysis method and testing system of Electronic Speckle Pattern Interferometry (ESPI) method, in measuring two - dimensional in-plane displacement. The anyalysis result of measurement by ESPE is quite comparable to that tof measurement by strain gauge method. This implies that the method of ESPE is a very effective tool in non-contact two-dimensional in-plane strain analysis. But there is a controversal point, measurment error. This error is discussed to be affected not by ESPE method itself, but by its analysis scheme of the interference fringe, where the first-order interpolation has been applied to the points of strain measured. In this case, it is turned out that the more errors would be occurred in the large interval of fringe. And so this paper describes a computer method for drawing when the height is available only for some arbitrary collection of points. The method is based on a distance-weighted, last- squares approximation technique with the weight varying with the distance of the data points.

• Design & Manufacturing
• /
• 제7권1호
• /
• pp.11-18
• /
• 2013

This paper presents the plastic inhomogeneous deformation behavior of bimetal composite rods during the axisymmetric and steady-state extrusion process through a conical die. The rigid-plastic FE model considering frictional contact problem was used to analyze the co-extrusion process with material combinations of Cu/Al. Different cases of initial geometry shape for composite material were simulated under different conditions of co-extrusion process, which includes the interference and frictional conditions. The main design parameters influencing on deformation pattern are diameter ratio of the composite components and semi-die angle. Efforts are focused on the deformation patterns, velocity gradient, predicted forming load and the end distance through the various simulations. Simulation results indicate that there is an obvious difference of forming pattern with various diameter ratio and semi-die angle. The analysis in this paper is concentrated on the evaluation of the design parameters on the deformation pattern of composite rod.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 1999년도 춘계학술대회 논문집
• /
• pp.704-707
• /
• 1999

Partial discharge(PD) in air insulated electric power systems is responsible for considerable power lossesfrom high voltage transmission lines. PD in air often leads to deterioration of insulation by the combined action of the discharge ions bombarding the surface and the action of chemical compounds that are formed by the discharge and may give rise to interference in ommunication systems. PD can indicate incipient failure. Thus understanding and classification of PD in air is very important to discern source of PD. In this paper, we investigated PD in air by using statical method. We classified air discharge with corona, surface discharge and cavity discharge by source of discharge. we used the mean pulse-height phase distribution $H_{qmean}(\psi)$, the max pulse-height phase distribution $H_{qmax}(\psi)$ , the pulse count phase distribution $H_n(\psi)$ and the max pulse height vs. repetition rate $H_{q}(n)$ for analysis PD pattern. We used statistical operators, such as skewness(S+. S-1, kurtosis(K+, K-), mean phase(AP+. AP-), cross-correlation factor(CC) and asymmetry from the distribution.