• The Plant Pathology Journal
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• v.29 no.4
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• pp.454-459
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• 2013

The Potexvirus Alternanthera mosaic virus (AltMV) has multifunctional triple gene block (TGB) proteins, among which our studies have focused on the properties of the TGB1 protein. The TGB1 of AltMV has functions including RNA binding, RNA silencing suppression, and cell-to-cell movement, and is known to form homologous interactions. The helicase domains of AltMV TGB1 were separately mutated to identify which regions are involved in homologous TGB1 interactions. The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. Helicase motif I of AltMV TGB1 was found to be critical to maintain homologous interactions. Mutations in the remaining helicase motifs did not inhibit TGB1 homologous interactions. In the absence of homologous interaction of TGB1, subcellular localization of helicase domain I mutants showed distinctively different patterns from that of WT TGB1. These results provide important information to study viral movement and replication of AltMV.

• Nuclear Engineering and Technology
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• v.53 no.3
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• pp.1020-1028
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• 2021

The nuclear reactor coolant pump transferring heat energy inherently brings with it the unsteady flow and inevitably threatens to the safe operation of the pump unit, especially with the pressure pulsation induced by the rotor-stator interaction. In this paper, the characteristics of pressure pulsation of the diffuser in a nuclear reactor coolant pump were investigated by the numerical simulation with experimental validation. Pressure pulsation signals measured synchronously from sensors mounted on the radial diffuser of a model pump were analyzed via Welch's method. Frequency components induced by the rotor-stator interaction can be revealed by the diameter mode analysis method. The pressure pulsation of the diffuser is dominated by the blade passing frequency and its harmonics, which are free from the effect of flow rate and rotational speed while the corresponding amplitudes are easily affected by different operational conditions and measuring positions. The non-uniformity is much more affected by the rotational speed than the flow rate. This research is helpful for further work to reduce the pressure pulsation for the reactor coolant pump.

• Journal of the Korean Chemical Society
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• v.61 no.3
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• pp.104-111
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• 2017

In this study, we analyzed verbal interactions in small group science learning using smart devices by the level of prior achievement. Four heterogeneous groups at a coed high school in Seoul participated. Verbal interactions during small group science learning were audio- and video-taped, transcribed, and analyzed. Verbal interactions were analyzed at the levels of a turn and an interaction unit. The results revealed that the frequencies of verbal interactions were high in task category, especially at information explanation, information question, and reflection on standards subcategories. Furthermore, the frequencies of high-level students at direction explanation, reflection on standards and progress subcategories were higher than those of low-level students, and the frequencies of low-level students at direction question and information explanation subcategories were higher than their counterpart. In the analyses of the interaction units, the frequencies in symmetric elaborated interaction were high, especially at cumulative and evaluative subcategories.

• Interaction and multiscale mechanics
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• v.2 no.4
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• pp.397-411
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• 2009

The effect of soil-structure interaction on a simple single storeyed and two bay space frame resting on a pile group embedded in the cohesive soil (clay) with flexible cap is examined in this paper. For this purpose, a more rational approach is resorted to using the three dimensional finite element analysis with realistic assumptions. The members of the superstructure and substructure are descretized using 20 node isoparametric continuum elements while the interface between the soil and pile is modeled using 16 node isoparametric interface elements. Owing to viability in terms of computational resources and memory requirement, the approach of uncoupled analysis is generally preferred to coupled analysis of the system. However, an interactive analysis of the system is presented in this paper where the building frame and pile foundation are considered as a single compatible unit. This study is focused on the interaction between the pile cap and underlying soil. In the parametric study conducted using the coupled analysis, the effect of pile spacing in a pile group and configuration of the pile group is evaluated on the response of superstructure. The responses of the superstructure considered include the displacement at top of the frame and moments in the superstructure columns. The effect of soil-structure interaction is found to be quite significant for the type of foundation used in the study. The percentage variation in the values of displacement obtained using the coupled and uncoupled analysis is found in the range of 4-17 and that for the moment in the range of 3-10. A reasonable agreement is observed in the results obtained using either approach.

• Structural Engineering and Mechanics
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• v.41 no.6
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• pp.711-734
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• 2012

The foundation of a tall building frame resting on settable soil mass undergoes differential settlements which alter the forces in the structural members significantly. For tall buildings it is essential to consider seismic forces in analysis. The building frame, foundation and soil mass are considered to act as single integral compatible structural unit. The stress-strain characteristics of the supporting soil play a vital role in the interaction analysis. The resulting differential settlements of the soil mass are responsible for the redistribution of forces in the superstructure. In the present work, the nonlinear interaction analysis of a two-bay ten-storey plane building frame- layered soil system under seismic loading has been carried out using the coupled finite-infinite elements. The frame has been considered to act in linear elastic manner while the soil mass to act as nonlinear elastic manner. The subsoil in reality exists in layered formation and consists of various soil layers having different properties. Each individual soil layer in reality can be considered to behave in nonlinear manner. The nonlinear layered system as a whole will undergo differential settlements. Thus, it becomes essential to study the structural behaviour of a structure resting on such nonlinear composite layered soil system. The nonlinear constitutive hyperbolic soil model available in the literature is adopted to model the nonlinear behaviour of the soil mass. The structural behaviour of the interaction system is investigated as the shear forces and bending moments in superstructure get significantly altered due to differential settlements of the soil mass.

• Child Health Nursing Research
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• v.15 no.1
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• pp.53-60
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• 2009

Purpose: The purpose of this study was to analyze metacommunicative episodes comprised of nurses' metacommunicative behavior and children's responses occurring in the dyads of nurse-child within the context of an inpatient pediatric unit. Methods: Twelve dyads of nurses and children were videotaped for four hours each day over a two-day period as they interacted with each other on the inpatient unit. The metacommunicative episodes were recorded from the videotapes. The metacommunicative behavior was categorized within Shin's metacommunicative behaviors. Results: The total number of episodes between the nurses and children included in this study was 242. The most frequently used metacommunicative behavior was 'reflection', followed by tagging, baby talk, approaching, mediating eye level, friendly demand, encouraging, description of acts, symbolization, turnabouts, touching, and mimic voice, respectively. The most common response to the nurses' metacommunicative behavior was agreeing, followed by tension release. Conclusion: Young children responded positively to metacommunicative behavior by the nurses. Understanding metacommunicative behavior may help nurses approach their young patients more easily and with confidence.

• Biomedical Science Letters
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• v.12 no.2
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• pp.127-130
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• 2006

It is important to coordinated interaction among neurons, astrocytes and endothelial cells to maintain the function of brain. To study their regulatory mechanisms in vitro system, the co-culture system among the isolated cells from brain may be needed. However, the method for purifying brain microvascular endothelial cells (BMEC) far culture have not established yet. In this study, the proper culture methods of mice cells using two different strains, CD1 and C57BL6, to obtain the pure and plentiful endothelial cells were described. The flatted-round forms of CD1 endothelial cells grew on the collagen-IV coating plates, while the purified cells from C57 mice preferred type collagen-I dishes for their growth. Both cells displayed anti-PECAM-1 (CD31) and von Willebrand Factor immune-reactivity. These results indicated that different coating materials not only improve attachment of isolated cells but also promoting growth of cells, suggesting that this method of purifying murine Brain microvascular endothelial cells (BMEC) provides a suitable model to investigate blood-brain-barrier (BBB) properties within neurovascular unit in vitro.

• Journal of the Korean housing association
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• v.5 no.2
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• pp.141-148
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• 1994

The behavioral-related environmental study for multifamily housing is needed to develop the quality of unit floor plan in terms of user comfort and needs. So, the aim of this study is to find out the living style and housing needs in terms of interaction between the users behavior pattern and orgarnization of physical home space. For the purpose, the field experiments in Pusan, have been carried out with 318 households living in apartments by plan types(2BL, 3BL, 4BL, 5BL) which were classified by number of bedrooms. The dwelling space usage behavior(room use pattern and furniture uasge pattern) and the space adjustment behavior(physical modification of space and preferred space adjustment attitude) were examined and analyzed.

• 한국전산유체공학회:학술대회논문집
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• 2001.10a
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• pp.106-111
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• 2001

This paper presents the numerical methodology of ATHOS3 code for thermal hydraulic analysis of Pressurized Water Reactor (PWR) steam generators. Topics include porous media approach, governing equations, physical models and correlations for solid-to-fluid interaction and heat transfer, and numerical solution scheme. The ATHOS3 code is applied to the thermal hydraulic analysis of steam generator in the Korea Kori Unit-1 nuclear power plant and the computed results are presented.

• Interaction and multiscale mechanics
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• v.1 no.4
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• pp.467-476
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• 2008

In this work, a nano-size rocksalt crystal (magnesium oxide) is considered as a continuous collection of unit cells, while each unit cell consists of discrete atoms; and modeled by a multiscale concurrent atomic/continuum field theory. The response of the crystal to an electromagnetic (EM) wave is studied. Finite element analysis is performed by solving the governing equations of the multiscale theory. Due to the applied EM field, the inhomogeneous motions of discrete atoms in the polarizable crystal give rise to the change of microstructure and the polarization wave. The relation between the natural frequency of this system and the driving frequency of the applied EM field is found and discussed.