• Title/Summary/Keyword: intensive culture

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Clinical Review of Pediatric Adenoviral Lower Respiratory Infection (아데노바이러스에 의한 소아 하기도 감염에 대한 임상적 고찰)

  • Son, Jin-A;Lee, Sang-Il;Lee, Nam-Young;Kim, Jung-Hee
    • Pediatric Infection and Vaccine
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    • v.3 no.2
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    • pp.154-162
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    • 1996
  • Purpose : Adenoviruses(Ad) have been shown to play an important role in the etiology of severely acute respiratory diseases, particulary in infants and young children, and the occurrence of fatal outcome and chronic pulmonary sequelae in association with adenoviral infection has been a cause of great interest and concern. This report presents the resul of a retrospective analysis on 30 cases of lower respiratory infection from which adenovirus was isolated. Patients & Methods : The 30 patients in this study represent all detected cases of adenovial infection out of 240 children who were admitted to Sang Sung Medical Center between February to June 1996 showing signs and symptoms of lower respiratory tract infection. The diagnosis of adenovirus infection was based on microscopic visualization of typical cytopathic effect in HEp-2 tissue culture and used monoclonal Ab with nasopharyngeal aspiration. Results : The male/female ratio was 2:1 and the majority of age range was below 36months. Clinical diagnoses in all 30 patients were pneumonia(n=21), bronchitis and Bronchiolitis(n=5) and ARDS(n=4). We recieved the most of patients in the month of May. The chief complaints were fever(93.3%) and cough(80%) and extrapulmonary symptoms were diarrhea(n=5), seizure(n=4), abdominal pain(n=1). The mean duration of fever was $11.95{\pm}6.54$days. Physical examination on admission were crackles(73.3%), coarse breathing sounds(60%), hepatosplenomegaly(33.3%), decreased brething sounds(30%). In WBC counts, 8cases were below $4000/mm^3$ and 14 cases were above $10,000/mm^3$. In platelets counts, 4cases were below $150,000/mm^3$ and 10 cases were above $450,000/mm^3$. 21 cases were above 1 in CRP. GOT and GPT were abnormal in some cases. Chest X-ray revealed diffuse pulmonary infiltration(n=15), pleural effusion(n=6), consolidation(n=4) and hyperaeration(n=3). Seven patients were treated at the peditric intensive care unit with respiratory support and high dose of gammaglobulin. However, one patients died even through he was treated with NO ventilation and high frequency ventilation. Conclusion : Those with adenoviral pneumonia and respiratory infection having long fever duration and symptoms like bacterial pneumonia must be carefully differentiated in order to provide proper treatement and preventive measures due to possible fatal outcome.

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An Exploratory Study on Gotcha Journalism (가차 저널리즘(gotcha journalism), 탐색적 연구: 노무현정부 출범 이후 정치보도를 중심으로)

  • Kim, Dong-Yule
    • Korean journal of communication and information
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    • v.29
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    • pp.43-71
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    • 2005
  • The study attempts to introduce 'Gotcha Journalism' to the Korea journalism, research and analyze it. Gotcha journalism is the journalistic reporting attitude that the journalists repeatedly and intensively reports public figures, especially politicians' mistakes or happening. 'Gotcha' is abbreviation of "I got you", frequently used in the English and American culture. In order to get the goal of this study, several journalists who are currently working for Chosun, Joongang, Dong-a, Hangyore, Kyunghyang and Seoul, national covering daily newspapers. In addition, the reporters who work for KBS, MBC, and SBS were Interviewed. All journalists who have been worked more than 5 years at the politic department were selected as interviewees. Thus, Intensive interviews are prime source of this study. Based the result of the research, gotcha journalism is externally derived from obsessive interest of Korean people to politics. Internally, it is easy to report happening or mistakes rather than big argument or ideological agenda of the politicians as news for journalists and to catch readers. In addition, the competition with advertising income accelerates this situation. And gotcha journalistic reporting behavior or attitude highly relates to political propensity of the newspapers or broadcastings. Especially some of the newspapers take gotcha journalism as a political struggling tool. It is appeared that another major cause for gotcha journalistic reporting behavior is customer-driven news production.

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Risk based policy at big data era: Case study of privacy invasion (빅 데이터 시대 위험기반의 정책 - 개인정보침해 사례를 중심으로 -)

  • Moon, Hyejung;Cho, Hyun Suk
    • Informatization Policy
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    • v.19 no.4
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    • pp.63-82
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    • 2012
  • The world's best level of ICT(Information, Communication and Technology) infrastructure has experienced the world's worst level of ICT accident in Korea. The number of major accidents of privacy invasion has been three times larger than the total number of Internet user of Korea. The cause of the severe accident was due to big data environment. As a result, big data environment has become an important policy agenda. This paper has conducted analyzing the accident case of data spill to study policy issues for ICT security from a social science perspective focusing on risk. The results from case analysis are as follows. First, ICT risk can be categorized 'severe, strong, intensive and individual'from the level of both probability and impact. Second, strategy of risk management can be designated 'avoid, transfer, mitigate, accept' by understanding their own culture type of relative group such as 'hierarchy, egalitarianism, fatalism and individualism'. Third, personal data has contained characteristics of big data such like 'volume, velocity, variety' for each risk situation. Therefore, government needs to establish a standing organization responsible for ICT risk policy and management in a new big data era. And the policy for ICT risk management needs to balance in considering 'technology, norms, laws, and market'in big data era.

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The Life Course Events and the Career Interruption among Korean Women (여성의 경력단절 기간별 생애사건 효과분석)

  • Min, Hyun-Joo
    • Korea journal of population studies
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    • v.34 no.1
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    • pp.53-72
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    • 2011
  • This study analyzes the effects of life course events and labor market conditions on the duration of career interruption among Korean women. The data were drawn from 'A Survey on the Women's Employment Interruption in Korea' conducted by the Korean Ministry of Gender Equality in 2009(currently the Korean Ministry of Gender Equality and Family). This study categorizes the duration of career interruption into three categories: (1) short term interruption(less than 12months), (2) short-medium term interruptio(12-35 months), (3) intermediate term interruption(36-59 months), (4) long term interruption(longer than 36 months), and then analyzes how demographic factors, labor market condition, and life events shape the timing of re-entry into the labor market among women. According to the findings, the jobs that are conducive to combining market work and mother's role expedite women's return to the labor market. Further, the younger, higher the level of human capital, and higher monthly wages that women earned before leaving the labor market, women are likely to experience short-term interruption(less than 12 months) rather than long-term interruption(longer than 60 months). Women who left from the labor market to care for kids are also likely to return to the labor market. However, women who have preschoolers are likely to experience long-term career interruption. These findings highlight the role of family supportive culture at the workplace in order for women to continue their employment while intensive family formation period. Furthermore, the finding that the discriminatory practices against women, in particular mother workers at the workplace lead women to exit from the market work calls for attention to establish family friendly workplace.

An Experimental Study on the Occurence of Bacteriuria according to Duration of Insertion, Frequency of Bladder Irrigation & Perineal Care, & Administration of Antibiotics in Patients with Indwelling Catheter (수직도뇨관환자의 요로감염발생요인에 관한 실험적 연구 -요관삽입기간, 삽입방법, 세척횟수, 회음부소독유무, 항생제사용유무를 중심으로 -)

  • 임난영;김분한
    • Journal of Korean Academy of Nursing
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    • v.11 no.1
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    • pp.19-27
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    • 1981
  • This Study was conducted at Intensive Care Unit of H & S Hospitals from Jan 4 to April 7, 1981 on 14mail & 26female adult patients. Each patient was screened and found to have nonbacteriuria in clean catch specimen before catheterization. Clean catch apecimen through Foley catheter were obtained after 24hours, 48hours and 72hours from catheterization. The result of this study is reviewed in a statistical analysis of percentage & Chi Square test to obtain the following findings. 1) The occurenc of bacteriuria in patients according to duration of indwelling catheter. a. 9.1% of the patient showed evidence of bacteriuria 24hours post catheterization specimen and 60% showed 48hours post cathetreization, while 68.4% of the patient showed evidence of bacteriuria 72hours post catheterization specimen. The occurence of bacteriuria in patients were significant differences at 1% level between duration of indwelling catheter. b. Mail patients had no infection 24hours post catheterization, 50% displayed bacteriuria 48hours post catheterization & 62.5% displayed bacteriuria 71hours post catheterization. 11.1% of femail patients displayed infection 24hours post catheterization 66.7% displayed infection 48hours post catheterization and 72.7% displayed infection 72hours post catheterization. There were significant differences at 1% level between bacteriuria occurence of mail & femail patients and the duration of insertion. 2) 56% of those patient who have altered mental state developed bacteriuria, while 40% of those patient who have alear mental state developed bacteriuria. But there was without statistically any significant difference between patient's mental status. 3) The occurence of bacteriuria with the administration of antibiotics in 36 patient was in 50%. The occurence of bacteriuria without the administration of antibiotics in 4 patients was in 50%. But there was without statistically any significant difference between the administration of antibiotics. 4) The occurence of bacteriuria in patients according to frequency of bladder irrigation. 50% of those patient who irrigated twice a day developed bacteriuria, 63.6% of those patient who irrigated once a day developed bacteriuria. The occurence of bacteriuria in patients were significant differences at 1% level between frequency of bladder irrigation. 5) The occurence of bacteriuria in patients who did perineal care once a day was 58.1%, 22.6% of those patient who did perineal care twice a day developed bacteriuria. But there was without statistically any signiticant differences between frequency of perineal care. 6) Most frequent bacteria of all bacterial strains isolated by culture of the urine was E. coli(45%). Enterococci & Staphylococcus were 15% respectively.

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Developmental Expression of Neurofilament 3 (NF-M) in the Cultured Rat Cortical Neurons (배양한 흰쥐 대뇌신경세포에서 신경미세섬유 3(NF-M)의 발생학적 표현)

  • Jung Jae-Seob;Cho Sun-Jung;Jin IngNyol;Jung Seung Hyun;Moon Il Soo
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.600-606
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    • 2005
  • Neurofilament (NF) proteins constitute the major intermediate filament type in adult neurons. They are made up by the copolymerization of the neurofilament light (NF-L, 61 kDa), medium (NF-M, 90kDa), and heavy (NF-H, 115 kDa) proteins. Although neurofilaments play a crucial .ole in neuronal growth, organization, shape, and plasticity, their expression pattern and cellular distribution in the developing neurons remain unknown. In this study, we have produced a rabbit polyclonal antibody specific to NF-M and investigated expression of NF-M in cultured cortical neurons. Immunostaining of 12 and 24 h cultures revealed strong expression of NF-M in axonal growth cone and in the region of a soma toward the axon. Doublestaining of 4 and 14 DIV corical neurons with NF-M and PSD95 antibodies revealed that both axon and dendrites were stained intensely with NF-M antibody, and that NF-M immunostaining along dendrites is often punctate and colocalize with PSD95 puncta, indicating that the puncta represent postsynaptic spines. Presence of NF-M in the postsynaptic spine was also indicated by immunoblot analysis of the postsynaptic density fraction. Taken together, our results show intensive targeting of NF-M into axons in the early axonal development, and into spines in mature neurons, indicating its important functions in axon and spine development.

Breeding of Phalaenopsis 'SM 333' with Mini Multiple Flower Formation (소형 다화 분지성 호접란 'SM 333' 육성)

  • Park, No Eun;Son, Beung Gu;Kim, Hong Yul;Lim, Ki-Byung
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.149-154
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    • 2015
  • A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.

Infestation of the Abalone, Haliotis Discus Hannai, by the Polydora under Intensive Culture Conditions in Korea (우리나라 전복 양식장의 패각 천공성 다모류 감염현황)

  • Won, Kyoung-Mi;Kim, Byeng-Hak;Jin, Young-Guk;Park, Young-Jin;Son, Maeng-Hyun;Cho, Mi-Young;Park, Myoung-Ae;Park, Min-Woo
    • Journal of fish pathology
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    • v.26 no.3
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    • pp.139-148
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    • 2013
  • The genus Polydora(Polychaeta, Spionidae) includes many species well known for their activity as borers. They often become harmful invaders by reducing the growth rate and meat yield of, or inducing the mortality of commercially important mollusck, abalone, Haiotis discus hannai. In 2012, the frequency of the Polydora was observed with 5~99% in live abalone and 5.3~70.3% in dead abalone shells of abalone sea-caged aquaculture system, Korea. There are many nacreous blister in the ventral margin and inner of the infestated abalone by abalone in response to the Polydora. A worm bored two holes in the shell and come in and out for ingestion the organic matters. They are more than 40 mm in length and had outstanding palps with black bars, disc form pygidium and 4 eyespots. This is the first record for the statue of Polydora infestation of sea-caged cultured abalone, Haliotis discus hannai in Korea.

The Use of Information and Communication Technologies in Education of Students' Civic Responsibility

  • Sadovyi, Mykola;Terenko, Olena;Filimonova, Tetiana;Malanchuk, Serhii;Vovkochyn, Lyudmyla;Paslawska, Alla;Oros, Ildiko
    • International Journal of Computer Science & Network Security
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    • v.22 no.7
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    • pp.213-219
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    • 2022
  • Building Ukraine as an independent, sovereign state requires the education of a citizen-patriot, able to live and work in a democracy, ensure the unity of Ukraine, feel constant responsibility for themselves, their people, country, seek to make a real contribution to the reform process. Modern modernization of the education system requires the search for new information and communication technologies that can ensure the formation of a citizen with an active civic position, which involves not only students mastering the rights and responsibilities of citizens, convincing them of the feasibility of democratic transformation of society, patriotic qualities and feelings, but also the identification of motivated civic actions. The pandemic and hostilities have led to significant changes in the field of education around the world, they have caused educational problems in Ukraine. At the beginning of the quarantine in the spring of 2020, all educational institutions in the emergency mode switched to distance learning. Intensive use of information and communication technologies in the life of modern society has led to a rethinking of the content of education and training of future professionals: the main role is played not so much by the information itself as the ability to work with it, critically comprehend and produce new knowledge; the main thing is not the amount of information, but its quality; information is needed for further practical application and transformation into knowledge, and the ability to work with information becomes one of the important competencies of the modern specialist in the new transformation of society: from information to the knowledge society. In this context, one of the main forms of training is distance learning, which is able to respond to the challenges of society. The main methodological positions that are taken into account in the construction of the structure and dynamics of the formation of civic responsibility of the individual during the use of information and communication technologies are highlighted. The structure of civil responsibility as a holistic system of information and communication technologies is outlined, which includes three subsystems that characterize the natural, social and systemic qualities of citizenship, interconnected hierarchically and synergistically.The constituent elements of the structural part of the model of civic culture of the individual are analyzed.

A simple mid-term preservation method (SMPM) of plant callus under low temperature conditions (저온 보존을 이용한 간편 중기 식물캘러스 저장법)

  • Park, Sung-Chul;Park, Su Hyun;Kim, Soyoung;Jeong, Yu Jeong;Kim, Cha Young;Jeong, Jae Cheol
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.187-192
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    • 2022
  • The repeated monthly or weekly subculture of plant callus is labor intensive and increases the risk of somaclonal variation from the parental callus line. The most effective method for preserving plant callus is cryopreservation, which involves storage in liquid nitrogen. However, this method cannot be applied to the callus of different plant species in the same manner, so it is difficult to develop a standardized cryopreservation method. In addition, the survival rate of the frozen callus after thawing and the regeneration rate after survival are uncertain. Therefore, it is necessary to develop a method to extend the subculture interval of plant callus in an active state. In this study, active plant calli of various species without freezing was incubated at 15℃ for 4 to 12 weeks without subculture. After 12 weeks, 8 lines of plant callus grew less than 2-fold when cultured at 25℃, but at least 2 times as much when cultured at 15℃. Moreover, total antioxidant activity did not differ significantly between plant callus recovered at 25℃ after culturing at 15℃ or at 25℃. These results show that the subculture interval can be extended at a temperature of 15℃ without need for modified medium composition or additional processes. In addition, positive results in all calli of several plant species are expected to reduce labor as well as somaclonal variation by increasing the subculture.