• The Korean Journal of Mycology
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• v.26 no.4 s.87
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• pp.450-457
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• 1998

The cryparin of Cryphonectria parasitica belongs to a cell wall associated fungal hydrophobin. The cryparin, though it is encoded by a single copy gene, is known for the high expression during the liquid culture of C. parasitica, and it turns out that 22% of total mRNA was transcribed for cryparin at 48hr after the liquid culture. In addition, it is also known as one of down-regulated fungal proteins by the presence of double stranded RNA virus, Cryphonectria hypovirus 1. In previous studies (Kim et al., 1999), we have constructed a cryparin-null mutant by replacing the cryparin gene with hygromycin B resistance gene due to site directed homologous recombination. In order for the promoter analysis of cryparin which seems to be very strong as well as mycoviral specific, it is preferable to have a strain with only a target promoter replaced and a discernable target site for incoming vectors. However, the cryparin-null mutant revealed the presence of an additional copy of transforming vector except the one which replaced the cryparin gene. In addition, the cryparin-null mutant did not contain any markers for targeted integration of incoming vectors. This prompts us to design an experiment to obtain a strain for promoter analysis of cryparin gene. A different mating type strain EP6(Mata, $met^-$) was mated with the cryparin-null mutant ${\triangle}$Crp194-7(MatA, Crp${\triangle}$::hph) to make the progenies with only a single replacement vector and $met^-$ characteristic remained. Nutritional assay as well as Southern blot analysis revealed that the progeny, ${\triangle}$Crp194-a6, was the methionine auxotroph with a single replacing vector in genome. Northern blot analysis and PAGE showed that there was no cryparin produced in this bred strain either.

• Journal of Distribution Science
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• v.13 no.1
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• pp.27-33
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• 2015

Purpose - The ICT market in the EU is lagging behind that of the US; however, algorithm and software development within the EU have grown steadily, and they involve focusing on the creative cultural convergence conceptualized as part of Horizon 2020 and connecting neighboring markets in the EE and the Mediterranean region. It is essential to study the requirements to market the EU's creative ICT development in emerging industrial countries after examining its applicability in these countries. Research design, data, and methodology - This study deals with data pertaining to the EU's creative industry and competitive edge. The global cultural expansion of the EU facilitates a new concept involving not only low-cost IT products to enhance local cultural artifacts through R&D and the construction of efficient infrastructure services, but also information exchange with a realistic commercialization of the technology that can be applied for creative cultural localization. In the European industry, research on algorithms has been applied for the benefit of consumers. We investigated how the process is conducted in the EU. Results - Europe needs to adjust its economic structure to the local culture as part of IT distribution convergence. The convergence has been converted into a production algorithm with IT in the form of low-cost production. This is because there is an attempt to improve the quality of transport infrastructure, workforce availability, and the distribution of the distance to the local industries and consumers, using IT algorithms. Integrated into the manufacturing industry, based on the ICT infrastructure and solutions, smart localized regional clusters are formed with the help of grafting. Europe has own strategy to increase the number of hub-and-spoke cities. Europe is now becoming integrated, with an EPC system for regional cooperation rather than national competition in ICT technology. Europe has also been recognized in this study as changing the step-by-step paradigm for global competitiveness through new creative culture industries. Conclusions - As a result, there are several ways of converging with others through EU R&D intensity; therefore, the EU can be seen as successfully increasing marginal value, which is useful in developing a special industrial cluster or local cultural cities that create converged development by connecting people and objects with IT. In fact, when compared to the US, Europe has a strong culture and the car industries have a tendency to overshadow the IT industries with integration of services in IT distribution. Considering the rapid environmental changes, the convergence of IT services is likely to take place in Europe, similar to the pharmaceutical industry and the automotive industry. This requires a focus on human resources and automated systems management. The trend is to move away from low-wage industries, switched to key personnel centers of the local university-industry. EU emphasizes the creation of IT market demand in Europe involving local cultural convergence for marketing as the second step to strengthen the economic hub-and-spoke areas.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.46
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• pp.29-60
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• 2010

The incentive and reasons to publish FIDIC Conditions of Contract for Design, Build and Operate Projects(DBO Form) are manifold. It is partly a response to the increasing need for sophisticated project delivery methods in both the public and private sectors and the already widespread use of the FIDIC Yellow Book with operation and maintenance obligations and partly a response to the challenge to decrease maintenance cost to a minimum by means of a new procurement route. As a result, FIDIC has developed a new model form to meet this market place requirement. On the other hand, FIDIC did not simply adapt the Yellow Book but has developed a new form from it, whilst preserving the style of the already known FIDIC Forms and maintaining the wording where it was not necessary to change it for the purposes of a DBO Form. Moreover DBO Form fills up supposed gaps in other FIDIC Forms and ameliorates the claim management and dispute management framework. FIDIC DBO approach may be shortly summarized as follows. First, DBO Form provides for single project responsibility. Second, DBO Form has the clear objective of ensuring the use of a most reliable and efficient technology at the lowest life-cycle cost. Third, DBO Form is intended to operate as an effective quality increase in the design and construction of projects. Fourth, DBO Form is intended to provide significant benefits with regard to system integration and reduction of risks. Fifth, DBO Form accelerates and enhances completion schedule compliance. Sixth, DBO takes care of all three supporting pillars of sustainability(including economical, environmental and social elements). DBO Form is obviously a good starting point for negotiations and the preparation of calls for tenders, thus saving the parties time and money. However, existing cultural and legal differences, particular local conditions and the particular needs of some branches of the industry may require the form to be adapted according to the particular needs of a project. And Civil law practitioners are strongly recommended to verify carefully the underlying legal concepts and background of each clause of the General Conditions in order to avoid unnecessary and sometimes unnatural changes and amendments being made. Note that when preparing the Particular Conditions ensure that terminology is consistent and that existing inherent concepts should not be ignored.

• Journal of the Korean Society for Railway
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• v.20 no.2
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• pp.257-264
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• 2017

The PC (Precast Concrete) construction method is a technique where concrete members that have been produced in a plant are constructed on site. Thus, continuity and secure integration of a structure that can be obtained by connecting rebars at splicing joint for PC members are the main areas of concern for this method. To evaluate the splicing and bonding performance according to application of a splice sleeve for connecting rebar in this research study, the diameter of rebar, development length, grouting strength etc. were set as variables. The performance and stiffness of splicing according to the development length of grout strength were measured and evaluated. In addition, by conducting comparative analysis on each of the variables, the factors that affected the splice sleeve for connecting rebar were discussed. The results confirmed that the strength and stiffness of the splice sleeve for connecting rebar were significantly affected by the development length while the increase in performance according to grout strength was not as significant.

• Journal of the Korea Convergence Society
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• v.12 no.8
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• pp.131-144
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• 2021

In recent years, China has gradually made clear its decision to modernize the governance system and governance capacity of the government by the virtue of digital transformation. As for the smart prison, as a penal institution of the state, technological reform is a key element in the sustainable development of smart prisons; however, relying on technology does not necessarily lead to a better service experience. Service design concept, as a coordinator of technology and social sustainability, needs to be adapted to the technological integration of smart prisons and to the needs for service design in the prison context in a new mode of thinking about services. This paper takes the development of the Jail Pay financial services system, one of the twelve sub-systems of the Smart Prison, as an entry point to explore the characteristics and shortcomings of the service design approach in achieving sustainable service innovation in the Smart Prison, it proposes an experience-based lead collaborative design (EBLCD) that is suitable for the specific needs in the prison context. The EBLCD is a theoretical framework and practical experience for sustainable service innovation in the construction of smart prisons.

• The Journal of the Korea Contents Association
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• v.21 no.5
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• pp.889-904
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• 2021

The planning of traditional urban has to face great challenges under the influence of the uncertainty and mobility of contemporary cities. While for this kind of challenge, it has brought the chance to develop Landscape Urbanism quickly. As an important practice place for Landscape Urbanism, open space provides a platform for many landscape designers to display. The purpose of this research is to explore the expression of the core content of Landscape Urbanism in open space, and propose the design tendency of open space in Korea. According to the constitution elements of urban open space and the core concept of Landscape Urbanism, this thesis establishes the analysis framework, which carries out the case empirical analysis for the open space of New York. Through case analysis, we can see that there are five major characteristics of Landscape Urbanism. That is, the integration or imitation of natural terrain, green infrastructure construction, emphasizing ecological resilience, adaptability to unplanned events, and analyzing the site from multiple scales. In this research, the design proposal proposed on the basis of Landscape Urbanism is able to provide enlightenment for the urban open space design of Korea in the future.

• Economic and Environmental Geology
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• v.46 no.5
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• pp.469-476
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• 2013

With the advent of a new Korean government, a new national agenda (140 items) was reported. Additionally, Work Plans of the executive bodies were also released, including the Ministry of Science, ICT and Future Planning (MSIP); the Ministry of Trade, Industry and Energy (MOTIE); the Ministry of Oceans and Fisheries (MOF); and the Ministry of Environment (MOE); the Ministry of Land, Infrastructure, and Transport (MOLIT). For government-supported research institutes, it will be important to watch and analyze the changes in the government's primary policies and strategic goals. In this paper, we analyzed new governmental research and development (R&D) policy with respect to geoscience and mineral resources technology. The results indicated that the executive bodies emphasized, for the job creating 'creative economy' and the 'safety and integration society', the establishment of the creative economy; science and technology development based on creativity and innovation; creation of new growth engines by fusion and diffusion; construction of stable energy systems; promotion of environmental industry; and creating satisfactory land services. In the area of geoscience and mineral resources technology, it is time to search for a new, creative, and interagency fusion R&D contents to meet the needs of the public and boost its national competitiveness.

• Communications of Mathematical Education
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• v.30 no.2
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• pp.161-178
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• 2016

Mathematics problem based learning(PBL), which has recently attracted much attention, is a teaching and learning method to increase mathematical ability and help learning mathematical concepts and principles through problem solving using students' mathematical prerequisite knowledge. In spite of such a quite attention, it is not easy to apply and practice PBL actually in school mathematics. Furthermore, the recent instructional situations or environments has focused on student's self construction of their learning and its process. Because of this reason, to whom is related to mathematics education including math teachers, investigation and recognition on the degree of students' acquisition of mathematical thinking skills and strategies(for example, inductive and deductive thinking, critical thinking, creative thinking) is an very important work. Thus, developing mathematical thinking skills is one of the most important goals of school mathematics. In particular, recently, connection or integration of one subject and the other subject in school is emphasized, and then mathematics might be one of the most important subjects to have a significant role to connect or integrate with other subjects. While considering the reason is that the ultimate goal of mathematics education is to pursue an enhancement of mathematical thinking ability through the enhancement of problem solving ability, this study aimed to implement basically what is the meaning of the integrated thinking ability in problem based learning theory in Mathematics. In addition, using historical materials, this study was to develop mathematical materials and a sample of a concrete instructional guideline for enhancing integrated thinking ability in problem based learning program.

• Journal of the Korean GEO-environmental Society
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• v.10 no.4
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• pp.49-57
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• 2009

The governmental investment to SOC facilities currently has increasedthe construction of new roads and the consistent extension of already-existing roads or the line-shape revision of those roads. As a result, the road cut slopes have been increasingrapidly. Unfortunately, human-life damages and property damages frequently occur due to the rockfall and the landslide every year. To reduce those damages, many studies have been performed. The present regulation regarding rockfall protection facilities follows the "Guide for Installation and Management of Road Safety Facilities" issued by MLTM (the Ministry of Land, Transport, and Maritime Affairs) that indicates the standard size of facilities and energy absorbing efficiencies. Most domestic road slopes use standardized rockfall protection facilities based on the regulation. However, there have been doubted about the effectiveness of rockfall protection facilities and the damages caused by rockfalls havebeen increasedevery year. Thus, it seems that relevant studies are necessary on the rockfall protection net being capable of supporting rockfall energies. Accordingly, this study reviews previous literature to investigate the function and the feature of rockfall protection nets and analyzetheir limitations by each type. After that, by using the pullout test device for a rockfall protection net, an experiment on the PVC coating net and the expanded metal is performed under the exact same condition. Finally, the features of the Expended metal is explained with the comparison analysises of load-variation and the confirmation of damaged forms. As a result, there have been founded the problemsof net breaking down and not being able to support due to PVC coating net's material property of disintegration. On the other hand, the Expanded Metal might be expected as a substituteof rockfall protection net according to its capability of support and integration.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.16 no.2
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• pp.251-258
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• 1998

The purpose of this study is to develop the District-level Planning Support System (DPSS) by using GIS. The district-level planning which is related for district-level control of city, needs the various parcel-level information which is composing the urban physical environment. The information has to be stored and analyzed for recognizing the study area, then the district-level planning will be efficiently managed. The use of GIS in the process of district-level planning is restricted for the creation of thematic map. GIS is not used for the analysis of spatial patterns and planning process. This study evaluates the characteristics of current district-level planning and the basic components of urban physical environment. And the database model is built. The topology among components is defined by using the spatial relationship. Then the spatial query machine for district-level planing is developed by using ArcView 3.1, Avenue and Dialog Extension. This spatial query machine is applied for case study. This study shows 1) the possibility of the district-level planning support system for analyzing spatial relationship, 2) the needs of the up-to-date topographic map showing current building's footlines and the complete integration with cadastral maps, it will reduce the uncertainty in the spatial decision making process, 3) the methodology for the construction of spatial decision making rules, 4) the further study for the using of raster, network, image and three dimension data.