• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.283-288
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• 1998

The bialaphos is a potent inhibitor of glutamine synthease in higher plants and is used as a non-selective herbicide. We have used the bialaphos resistant gene(Bar) encoding for an acetyltransferase isolated from Streptomyces hygroscopicus SF1293. Callus derived from mature seeds of rice(Oryza sativa L. cv. Dong Jin) were co-cultivated with Agrobacterium tumefaciens EHA101 carring a plasmid pGPTV-HB containing genes for hygromycin resistance (HygR) and Bar. Transgenic plants showing in vitro resistance to 50 mg/L hygromycin and 10 mg/L bialaphos were obtained by using a two-step selection/regeneration procedure. Transformation efficiency of rice was about 30% which was as high as reported in other dicotyledons. Progenies ($\textrm{T}_{1}$ generation) derived from primary transformant of 17 lines were segregated with a 3 resistant : 1 sensitive ratio in medium containing hygromycin and bialaphos. Stable integration of Bar gene into chromosomal DNA was proven by Southern blot analysis of genomic DNA isolated from $\textrm{T}_{2}$ progenies. Transgenic plants ($\textrm{T}_{3}$) grown in the field were resistant to bialaphos (Basta) at a dosage lethal to wild type plants.

• Journal of Life Science
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• v.23 no.2
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• pp.290-298
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• 2013

The genus Mycobacterium includes crucial animal and human pathogens such as Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium bovis. Although it is important to understand the genetic basis for their virulence and persistence in host, genetic analysis in mycobacteria was hampered by a lack of sufficient genetic tools. Therefore, many functional vectors as molecular genetic tools have been designed for understanding mycobacterial biology, and the application of these tools to mycobacteria has accelerated the study of mechanisms involved in virulence and gene expression. To overcome the pre-existing problems in genetic manipulation of mycobacteria, this paper reports new vector systems as effective genetic tools in Mycobacterium smegmatis. Three vectors were developed; pKOTs is a suicide vector for mutagenesis containing a temperature-sensitive replication origin (TSRO) and the sacB gene encoding levansucrase as a counterselectable marker. pMV306lacZ is an integrative lacZ transcriptional fusion vector that can be inserted into chromosomal DNA by site-specific recombination. pTnMod-OKmTs is a minitransposon vector harboring the TSRO that can be used in random mutagenesis. It was demonstrated in this study that these vectors effectively worked in M. smegmatis. The vector systems reported here are expected to successfully applicable to future research of mycobacterial molecular genetics.

• Journal of muscle and joint health
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• v.3 no.1
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• pp.50-62
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• 1996

The purpose of this study was to identify and construct a substantive theory about the experience of fatigue among the female elderly with osteoarthritis. The interviewees were 6 female elderly with osteoarthritis. Of them 3 were admitted General hospital, the remaining 3 didn't admitted hospital. The data were collected through in-depth interviews by the investigators from May 20th, 1995 to June 19th, 1995. The collected data were coded into concepts and categories according to Strauss & Corbin's grounded method. The data thus collected were analyzed immediatly after interviews and fed into next round of interviews until the data collection reaches the saturation point where no additional concepts emerge. The 35 concepts were from analyzing the grounded data. The results of this study were as follows : 'change of the health condition','change of the life pattern', 'physiologic change', 'change of the coping pattern', 'performance of homework', 'activity daily living', 'tireness', 'ache', 'change of general apperance', 'sleep disturbance', 'powerlessness'. 'Physiologic unbalance', 'change of the family dynamics', 'physical imbalance', 'loss of the control', aviodance', 'conversion', 'leaving', 'indifference', 'hesitation', 'the pursue of information and trial', 'the western therapy', 'heat therapy', 'the oriental therapy','exercise', 'weight control', 'support', 'solution', 'being as before', 'adding more'. Six categories emerged from the analysis of ground data. They were as follows : 'stimuli', 'fatigue', 'passive coping', 'active coping', 'disolution', 'remains'. 13 hypothesis were derived from the integration of categories as follows : 1. The stronger the stimulus as perceived by the subjects, the more the fatigue will exrerience. 2. The more severe the fatigue, the more severe the tireness. 3. The more severe the fatigue, the more the hardness. 4. The more severe the fatigue, the more the variability of appear will experience. 5. The more severe the fatigue, the more the pain will experience. 6. The more severe the fatigue, the more the insomnia will experience. 7. The more severe the fatigue, the more the powerless will experience. 8 The more severe the fatigue, the more the psychological unbalance will experience. 9. The more severe the fatigue, the more variability of the family dynamics will experience. 10. The more severe the fatigue, the more the physical unbalance will experience. 11. The more severe the fatigue, the more loss of control will experience. 12. The subjects who experienced the fatigue will be to show passive coping and active coping mechanism.

• Korean journal of food and cookery science
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• v.22 no.5 s.95
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• pp.666-680
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• 2006

To obtain basic data for the utilization of saltwort (Salicornia herbacea L.) as a functional ingredient in steamed foam cake, the optimum component ratios for major raw ingredients (saltwort, salt, and wheat flour) as independent variables that affect the product quality were scientifically determined using RSM (response surface methodology) technique. A three-factor and five-level rotational central composite design was used for treatment arrangement. The complete design consisted of 16 experimental points. The three independent variables selected for the RSM experiment were amounts of saltwort (X$_1$, 5${\sim}$25 g), salt (X$_2$, 0${\sim}$10 g), and wheat flour (X$_3$, 470${\sim}$530 g). The optimum responses in specific gravity of the batter and volume, color, texture, and sensory evaluation result of the cake were obtained. The specific gravity and viscosity of the batter at p<0.01 was verified from the regression curve. The characteristic of the batter was influenced by all independent variables, but was extremely dependent on the amount of saltwort ordinary points of the surface responses from the batter formed the minimum points for specific gravities of the batter while viscosities of the batter appeared with the saddle points. Analysis of the response indicated that the amount of saltwort was the most influential factor over the physical properties of the cake, among the dependent variables. Ordinary points of the surface responses from the cake formed the maximum points for loaf volume, hardness gumminess, and chewiness, while Hunter colorimetric parameters appeared with the saddle points. The result indicated that level of the saltwort deviating more or less from the optimal amount decreased the volume and increased the specific gravity with less tender product. Ordinary points of the surface responses of the sensory evaluation scores from the cake formed the maximum points for appearance, flavor, softness, and overall acceptability, while color values appeared with the saddle points. The result also indicated that the level of the saltwort deviating more or less from the optimal amount reduced the preference for the product. Integration of the optimum responses common to all dependent variables that overlapped all the contour maps finally indicated that the combination of 8.3${\sim}$13.8 g saltwort, 2.5${\sim}$6.6 g salt, and 486.5${\sim}$511.5 g wheat flour under the selected preparation recipe optimized the physical and sensory properties in the teamed foam cakes. Practical preparation of the product with median amounts of the ingredients, i.e., 11.0 g saltwort, 4.6 g salt, and 499.0 g wheat flour resulted in similar qualities to the predicted responses. In conclusion, these study results indicated that preparation of steamed foam cake with added saltwort ingredient could potentially produce a more nutritious product with less salt. Further research is required to acquire the optimum levels for sub-ingredients to improve the product quality.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.5
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• pp.115-123
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• 2009

Integrated information to water quantity and quality is essential for planning water resources management as well as operating water-related infrastructures. Because data collection process including monitoring and maintenance is separated in different governmental agencies in Korea, integrating quantity and quality may provide effective and better management implementation. In this study, a number of suggestions regarding integration of water monitoring were concluded in terms of technological, legal and institutional implications. First, it is necessary to discuss national water monitoring plan, national water information management plan, agreement of standard terms of monitoring between ministries, and to revise the law(river law and water quality management law). Present stations for water monitoring should be used for both of quantity and quality monitoring. If station is newly installed or relocated, it is better that one single agency maintain monitoring frequency and data management as well. In addition, a monitoring protocol need to be agreed by each of parties. In order to develop integrated monitoring system, quality assurance of the collected data should be properly maintained. Since many purposes haven been concerned using of data analysis and assessment so far, it may not be easy to integrate water quantity and quality monitoring in a short period. However, the alternatives including enhancing institutional regulations and programs, advanced technology may promote an efficient integrated water monitoring.

• Journal of Korean Home Economics Education Association
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• v.29 no.3
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• pp.105-124
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• 2017

This study aimed at developing an educational program for South Korean adolescents on North Korean family living culture so that the students become familiar with the values and everyday lifestyles of North Korea. Understanding of North Korean culture is considered important as a foundation for social integration in prospect of future reunification. The contents of the program were developed based on the analysis of North Korean defectors' interviews and review of the literature and media reports on North Korean family culture. The program consists of 12 units of 40-minute sessions with the following topics: economy and consumption, leisure activities, family relationships, dating and spouse choices, fashion changes, clothing acquisition and care, food choices and local diets, dietary problems, housing, and neighborhood. The program was implemented between December 21 and 30, 2015 in a middle school located in Seoul. This program is expected to be useful in preparing the students as future leaders to create harmonious family living culture in the reunified Korea.

• Korean Journal of Family Social Work
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• no.43
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• pp.37-59
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• 2014

The purpose of this study is to analyze and find the relative importance of the factors impacting on the intent to leave among the female care worker's the labor properties, Total 540 questionnaire were distributed and collected from 387 female care workers working at the area of Daegu and GyeongBuk. The logistic regression analysis was used to analyze the relative importance among the factors. The results of the study are the followings: Academic ability, lower subjective-evaluation of remuneration, non-business contracts, labor intensity, the relationship between the user, the relationship between the agency providing are factors influencing on the turnover intention. In other words, higher education, lower subjective-evaluation of remuneration, more work than the contract, higher labor intensity, the female care workers have higher probability of turnover intention. Based on the results of the study, this paper suggests some implications as policy and practice.

• Journal of The Geomorphological Association of Korea
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• v.20 no.1
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• pp.21-33
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• 2013

Weight of evidence model was applied for potential mapping of mountainous wetland to reduce the range of the field survey and to increase the efficiency of operations because the surveys of mountainous wetland need a lot of time and money owing to inaccessibility and extensiveness. The relationship between mountainous wetland location and related factors is expressed as a probability by Weight of evidence model. For this, the spatial database consist of slope map, curvature map, vegetation index map, wetness index map, soil drainage rating map was constructed in Yeongnam area, Korea, and weights of evidence based on the relationship between mountainous wetland location and each factor rating were calculated. As a result of correlation analysis between mountainous wetland location and each factors rating using likelihood ratio values, the probability of mountainous wetlands were increased at condition of lower slope, lower curvature, lower vegetation index value, lower wetness value, moderate soil drainage rating. Mountainous Wetland Potential Index(MWPI) was calculated by summation of the likelihood ratio and mountainous wetland potential map was constucted from GIS integration. The mountain wetland potential map was verified by comparison with the known mountainous wetland locations. The result showed the 75.48% in prediction accuracy.

• The Journal of the Korea Contents Association
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• v.19 no.10
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• pp.660-666
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• 2019

This study is about the research of recognition for deinstitutionalization and independent living of the disabled living in residential facilities for the people of family. The survey was conducted in October to September 2018 and 810 cases were used in the final analysis. In this study, we confirmed that about half of the families of groups and those who perceived In accordance with the enactment deinstitutionalization were divided into half. and consent to cegislation, local government efforts, independence, consent to deinstitutionalization family perceptions of disagreement of independence were identified as having no low information provided. These results indicate that there was a lack of provision of services or information related to independent living of the disabled living in residential facilities, and it was confirmed that proper information transfer education on policies and services is needed to have accurate understanding and awareness. In addition, the agreement on legislation, local government efforts, Independence, consent to deinstitutionalization were found to have a significant effect on preparation for independent living. Therefore, it was confirmed that systematic information education support are needed to ensure proper recognition of user's family in residential facilities and to ensure healthy independence of users.

• Korean Journal of Remote Sensing
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• v.35 no.5_2
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• pp.841-850
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• 2019

The purpose of this study is to strengthen the capability of rapid mapping for disaster through improving the positioning accuracy of mapping and fusion of multi-sensing point cloud data derived from Unmanned Aerial Vehicles (UAVs) and disaster investigation vehicle. The positioning accuracy was evaluated for two procedures of drone mapping with Agisoft PhotoScan: 1) general geo-referencing by self-calibration, 2) proposed geo-referencing with optimized camera model by using fixed accurate Interior Orientation Parameters (IOPs) derived from indoor camera calibration test and bundle adjustment. The analysis result of positioning accuracy showed that positioning RMS error was improved 2~3 m to 0.11~0.28 m in horizontal and 2.85 m to 0.45 m in vertical accuracy, respectively. In addition, proposed data fusion approach of multi-sensing point cloud with the constraints of the height showed that the point matching error was greatly reduced under about 0.07 m. Accordingly, our proposed data fusion approach will enable us to generate effectively and timelinessly ortho-imagery and high-resolution three dimensional geographic data for national disaster management in the future.