• 제목/요약/키워드: insight

검색결과 3,134건 처리시간 0.034초

Lactobacillus plantarum 299v Surface-Bound GAPDH: A New Insight Into Enzyme Cell Walls Location

  • Saad, N.;Urdaci, M.;Vignoles, C.;Chaignepain, S.;Tallon, R.;Schmitter, J.M.;Bressollier, P.
    • Journal of Microbiology and Biotechnology
    • /
    • 제19권12호
    • /
    • pp.1635-1643
    • /
    • 2009

  • The aim of this study was to provide new insight into the mechanism whereby the housekeeping enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) locates to cell walls of Lactobacillus plantarum 299v. After purification, cytosolic and cell wall GAPDH (cw-GAPDH) forms were characterized and shown to be identical homotetrameric active enzymes. GAPDH concentration on cell walls was growth-time dependent. Free GAPDH was not observed on the culture supernatant at any time during growth, and provoked cell lysis was not concomitant with any reassociation of GAPDH onto the cell surface. Hence, with the possibility of cw-GAPDH resulting from autolysis being unlikely, entrapment of intracellular GAPDH on the cell wall after a passive efflux through altered plasma membrane was investigated. Flow cytometry was used to assess L. plantarum 299v membrane permeabilization after labeling with propidium iodide (PI). By combining PI uptake and cw-GAPDH activity measurements, we demonstrate here that the increase in cw-GAPDH concentration from the early exponential phase to the late stationary phase is closely related to an increase in plasma membrane permeability during growth. Moreover, we observed that increases in both plasma membrane permeability and cw-GAPDH activity were delayed when glucose was added during L. plantarum 299v growth. Using a double labeling of L. plantarum 299v cells with anti-GAPDH antibodies and propidium iodide, we established unambiguously that cells with impaired membrane manifest five times more cw-GAPDH than unaltered cells. Our results show that plasma membrane permeability appears to be closely related to the efflux of GAPDH on the bacterial cell surface, offering new insight into the understanding of the cell wall location of this enzyme.

  • https://doi.org/10.4014/jmb.0902.0102 인용 PDF KSCI

조현병환자의 삶의 질에 미치는 영향요인 연구 (Schizophrenic Patients Impact on Quality of Life)

  • 김정숙
    • 융합정보논문지
    • /
    • 제8권1호
    • /
    • pp.53-58
    • /
    • 2018

  • 본 연구는 조현병환자의 삶의 질에 영향을 미치는 요인을 파악하기 위해 시도 되었다. 본 연구는 C시 지역에 거주하는 조현병환자 125명을 대상으로 한 서술적 조사연구이며 자료는 SPSS 21.0 을 이용하여 t-test, ANOVA, Pearson's correlation coefficients 산출 및 다중희귀분석을 실시하였다. 자료수집은 2016년 6월 1일부터 2016년 7월 31일 까지 였다. 본 연구결과는 다음과 같다. 조현병 환자의 일반적 특성에 따른 삶의 질 차이는 종교와 지지하는 팀의 종류에 따라 유의한 차이(p<.05)가 나타났다. 삶의 질은 자아존중감(r=.270), p<.0001), 가족지지(r=.348, p<.0001)와 유의한 양적 상관관계를 나타내었다. 자아존중감, 병식, 가족지지는 삶의 질을 49.5% 설명하였으며, 이중 가족지지와 자아존중감이 삶의 질에 의미 있는 영향을 주었다. 조현병 환자의 삶의 질 증진을 위해 가족지지와 자아존중감 강화를 위한 프로그램 개발이 요구된다.

  • https://doi.org/10.22156/CS4SMB.2018.8.1.053 인용 PDF KSCI