• Title/Summary/Keyword: insecticidal

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Occurrence of Thrips in Greenhouse Cucumber and Insecticidal Activity of Five Local Western Flower Thrips Populations (시설오이에서 총채벌레류 발생소장 및 5 지역계통 꽃노랑총채벌레의 약제반응)

  • Jeong, In-Hong;Park, Bueyong;Park, Se-Keun;Lee, Sang-Bum;Jeon, Sung-Wook
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.517-524
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    • 2018
  • The purpose of this study was to investigate the density of thrips, and insecticidal resistance for effective control of Western flower thrips in greenhouse. The presence and density of the thrips was investigated using yellow colored-sticky trap in a cucumber field from May to August in Cheon-an. The results of the investigation revealed the existence of the following thrips species; Frankliniella occidentalis, F. intonsa, Thrips palmi, T. tabaci, Scirtothrips dorsalis, Microcephalothrips abdominalis, and T. nigropilosus. The predominant pest was found to be the western flower thrips. To survey the western flower thrips insecticidal resistance, we established the discriminating concentration (DC), recommended concentration (RC) and $2{\times}$recommended concentration ($2{\times}RC$) of nine insecticides; Emamectin benzoate EC, spinetoram SC, Chlorfenapyr EC, Spinosad SC, Cyantraniliprole EC, Acetamiprid WP, Dinotefuran WG, thiacloprid SC and thiamethoxam SC. The bioassay of about five local populations was conducted using the leaf-dipping method. In all local populations, insecticidal resistance in western flower thrips had not developed in emamectin benzoate EC (RC, $10.8{\mu}L\;L^{-1}$), chlorfenapyr EC (RC, $50.0{\mu}L\;L^{-1}$), spinetoram SC (RC, $25.0mg\;L^{-1}$), and spinosad SC (RC, $50.0mg\;L^{-1}$). However, insecticidal resistance in RC was found to have developed in cyantraniliprole EC (RC, $50.0{\mu}L\;L^{-1}$) and four insecticides of neonicotinoid type. Insecticidal activity of 95% or more was observed in each population when cyantraniliprole EC tested in $2{\times}RC$. However, the neonicotinoid types showed different insecticidal activity in $2{\times}RC$.

Insecticidal Effect of Neem Cake Extracts on Cabbage Pests, Aphis gossypii and Pluetella xylostella

  • Lee. HoYong;Kim, Won-Rok;Min, Bong Hee
    • Korean Journal of Environmental Biology
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    • v.22 no.4
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    • pp.501-506
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    • 2004
  • In organic agriculture, choose of effective and cheap bio-pesticide is very important. The authors developed an insecticidal extract from neem cake, waste of neem oil from kernel, and applied as a bio-pesticide. Bio-pesticide neem cake extracts experiment on cabbage pest was carried out at Wonju Agricultural Technology and Extension Center from 11 March to 30 May 2003. There were six treatments with three replications, using completely randomized design. Treatments involved three and six sprays of synthetic pyrethroid pesticide cypermethrin 10 EC at the dilution rate of 2.2 mL $L^{-1}$ of distilled water and four, five and six sprays of bio-pesticide neem at the dilution rate of 13.3 mL $L^{-1}$ of distilled water, and untreated control. For each treatment, designated sprayings were done at 7 days interval. Pre-spray data showed that the plants in all the experimental plots were already infested with aphid (Aphis gossypii), and diamondback moth (Pluetella xylostella). The results indicated that all neem pesticide treatments were more effective in insecticidal activity than the untreated control and the chemical treatments in controlling aphids and diamondback moth. Among the three neem treatments, there were no significant differences between them.

Insecticidal Efficacy of Porteresia coarctata (Roxb.) on Bio-chemical Alteration of Spodoptera litura (Fab.)

  • Bhattacharyya Atanu;Goswami Arunava;Dihidar Shankar;Adhikari Sujit;Banerjee Sudip Kumar;Talapatra Soumendra Nath;Barik Bikas Ranjan;Bhattacharya Subir Kumar;Chandra Asit Kumar
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.1
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    • pp.9-13
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    • 2007
  • Hexane extract of Porteresia coarctata (Roxb.) exhibits a toxic effect on the tissues of Spodoptera litura (F) while fed at the dose of 1000 and 2000 ppm thoroughly mixing with castor leaves (Ricinus communis L) after dissolving in DMSO at late fourth instar whereas only DMSO treated castor leaves were fed to control group. The larvae were put to rear at $28^{\circ}C{\pm}1^{\circ}C$, $76{\pm}4%$ R.H. under 12 L + 12 D photoperiodic regime. In test group insects substantial reduction of protein and DNA content was marked in fat body and midgut tissues compared to DMSO treated control group. The significant biochemical alterations in the midgut tissues and fat body of test group insects indicate the insecticidal property of the said plant extract that could be tested in facilitating the phenomenal stride in Integrated Pest Management.

Transfer of Insecticidal Toxin Gene in Plants:Cloning of Insecticidal Protein Gene in Bacillus thuringiensis (식물세포에 살충독소 유전자의 전이: Bacillus thuringiensis 살충단백질 유전자의 클로닝)

  • 이형환;황성희;박유신
    • Microbiology and Biotechnology Letters
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    • v.18 no.6
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    • pp.647-652
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    • 1990
  • The production of delta-endotoxin crystal and the cloning of endotoxin protein gene in Bscillus thuringiensis subsp. kurstaki HD1 strain were studied. The strain produced bipyramidal crystals ($2.9\times 1.0 \mu m$) in their cells during sporulation. The B. thuringiensis contained about 10 plasmid DNA elements ranging from 2.1 to 80 kilobases. The 73 kb plasmid DNA, the 29 kb BamHI fragment and the 7.9 kb Pstl DNA fragment hybridized to the pHL probe. The 7.9 kb fragment was eluted and cloned in the PstI site of pBR322 vector and transformed into E. coli HB101, which produced insecticidal proteins killing Bornbyx mori larvae.

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Insecticidal Activity of Some Monoterpenoids (테르펜계 유기인 화합물의 살충성)

  • Lee, Young-Kyu;Song, Cheol;Kyung, Suk-Hun
    • Applied Biological Chemistry
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    • v.45 no.1
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    • pp.37-41
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    • 2002
  • Seven organophosphorus compounds and three carbamates of some monoterpenoids were synthesized and tested for insecticidal activity to five insects, brown plant happer (BPH, Nilaparvata lugens), green peach aphid (GPA, Myzus persicae), diamond bark moth (DBM, Plutella xylostella), tobacco cut worm (TCW, Spodoptera litura), and two spotted spider mite (TSSM, Tetranychus urticae). With the administration of 500 mg/l concentration thiophosphates of carveol and geraniol showed motality of from 95% to 100% to BPH and DBM. On the other hand, phosphates of menthol, thymol, and borneol had also good to moderate activity to BPH and TSSM insects at the same concentration.

Insecticidal Activity of Paecilomyces fumosoroseus SFP-198 as a Multi-Targeting Biological Control Agent against The Greenhouse Whitefly and The Two-Spotted Spider Mite

  • Kim, Jae-Su;Roh, Jong-Yul;Choi, Jae-Young;Shin, Sang-Chul;Jeon, Mun-Jang;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.2
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    • pp.181-187
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    • 2008
  • An entomopathogenic fungus, Paecilomyces fumosoroceus SFP-198 was isolated in Kyungbuk province, Korea and screened out for the control of Trialeurodes vaporariorum nymphs. It showed 72.5% efficacy against second instars of T. vaporariorum nymphs at 6 days after treatment in the laboratory. To select an active ingredient for the mass production and the formulation, SFP-198 culture products were compared based on their insecticidal activities against T. vaporariorum nymphs. Among them, conidia and blastospores showed much higher insecticidal activity than supernatants. Furthermore, SFP-198 conidia were more heat-resistant than blastospores, and also powder form of conidia was more stable than their suspension form. SFP-198 conidia showed high pathogenicity on not only T. vaporariorum but also Tetranychus urticae in the glasshouse. This result suggested that SFP-198 conidia can be used as a multi-targeting biological control agent against sucking agri- cultural pests, such as whiteflies and mites.

Insecticidal Effects of the Control Released Etofenprox Against Cotton Aphid (완효형 Etofenprox 제재의 목화진딧물에 대한 살충효과)

  • Chae, Si-Ra;Hwang, Chae-Eun;Seo, Mi-Ja;Yu, Yong-Man;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.37 no.3
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    • pp.361-365
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    • 2010
  • For the development of more effective insecticides for aphids, including the cotton aphid, Aphis gossypii Glover, control released insecticides were developed and tested as new type of formulation for the cotton aphid. Etofenprox is a target-of-control-released formulation that is toxic to insects with piercing-sucking type mouths including aphids and hoppers. The insecticidal effect of etofenprox is distinguished among others; however, continuance period of the effect of insecticide is shorter than other insecticides. In addition, the control effect of control released etofenprox on the cotton aphid was examined. The results show that control released etofenprox induced no control effects to the cotton aphid within 5 days of treatment. The cotton aphid's population was increased, and their reproduction rate was also high. However, control effect was shown to have very low levels 7 days after treatment, and it increased to higher effect. It is supposed that it is possible to control the cotton aphid using the type of control released etofenprox with a kitosan carrier. It is necessary to develop a more effective carrier type to release more of the insecticidal ingredient on the leaf after spraying.

Insecticidal Activity and Repellent Effects of Methylcinnamate Separated from Alpinia galangal on Ricania sp. (갈색날개매미충(Ricania sp.)에 대한 양강근 유래 Methylcinnamate의 살충 및 기피 효과)

  • Park, Bueyong;Jeong, In-Hong;Park, Se-Keun;Jeon, Sung-Wook;Lee, Sang-Bum;Lee, Sang-Ku;Lee, Sung-Eun
    • Korean Journal of Organic Agriculture
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    • v.27 no.1
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    • pp.57-63
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    • 2019
  • Ricania sp. is a pest to many crops in Korea. This pest prefers fruit crops especially, blueberry and apple trees. We tested the possibility of Ricania sp. control using of methylcinnamate. In the laboratory bioassay, the mortality of methylcinnamate against Ricania sp. adult with 100 and 250 time diluted solution was 36.6% and 13.3% respectively. While repellent that the use of methylcinnamate resulted rate of 43.3% with 100 time diluted solution and 40.0% in 250 time diluted solution. Insecticidal and repellent effect in semifield bioassay were higher than those in laboratory bioassay. From this result, methylcinnamate might have synergic effect for Ricania sp. management. The result of this study showed a possibility of Ricania. sp. control using methylcinnamate.

Convenient Bioassay Method and Insecticidal Susceptibility of the Black Cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) (검거세미밤나방(Agrotis ipsilon )의 간이생물검정법에 의한 약제 감수성)

  • Lee, Young Su;Lee, Hyun Ju;Choi, Jong Yoon;Lee, Sang-Woo;Lee, Young Soon;Lee, Hee A
    • Korean journal of applied entomology
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    • v.60 no.2
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    • pp.201-202
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    • 2021
  • We confirmed the convenient bioassay method for the black cutworm, Agrotis ipsilon in semi-field conditions. And we surveyed the susceptibility to commercial pesticides of A. ipsilon, which feeds Chinese cabbage through this bioassay process. As a result of the test, six soil mixing insecticides and eight soil drenching insecticides showed more than 90% insecticidal activity.

Chemical Characterization and Insecticidal Activity of Rubus coreanus Miquel Extracts (Leaves, Fruits and Stems) against Three Agricultural Insect Pests, Myzus persicae, Plutella xylostella and Spodoptera litura (특용작물:복분자의 화학적 특성 및 복분자 부위별 (잎, 열매, 줄기) 추출물을 이용한 농업해충 복숭아혹진딧물, 배추좀나방 및 담배거세미나방에 대한 살충효과 연구)

  • Lee, Hee-Kwon;Lee, Hoi-Seon
    • Korean Journal of Organic Agriculture
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    • v.24 no.4
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    • pp.849-857
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    • 2016
  • In the growth of the Rubus coreanus Miquel fruits, the unripened (10 days) and ripened (25 days) fruits after flowering were harvested. The chemical characteristics of different maturational stages of the unripened and ripened fruits were investigated. Total amount of phenolic compounds was 4.00-7.56% in the unripened fruits and 3.78-5.57% in the ripened fruits, respectively. Furthermore, total amounts of organic acids such as malic, citric, succinic, and oxalic acids were 16.40 mg/100 g in the unripened fruits and 28.82 mg/100 g in the ripened fruits, respectively. In organic acids of the unripened and the ripened fruits, citric acid (8.76-15.47 mg/100 g) was the highest amount among other organic acids. Soluble sugars were significantly increased from 11.07 to 21.54% in the unripened and ripened fruits. Therefore, ripened fruits had the high levels of phenolic compounds, organic acids and soluble sugars. For the biological studies of R. coreanus, methanol extracts of R. coreanus leaves, fruits, and stems were evaluated for their insecticidal activities against Myzus persicae (Hemiptera: Aphididae), Plutella xylostella (Lepioptera: Plutellidae) and Spodoptera litura (Lepioptera: Noctuidae) by leaf dipping method. From these results, the extract of R. coreanus leaves revealed potent insecticidal activity against P. xylostella. However, the methanol extracts of R. coreanus fruits and stems had no any insecticidal activity against M. persicae, P. xylostella and S. litura. The R. coreanus leaves have promising potential as new insecticidal agent against P. xylostella.