• 제목/요약/키워드: inorganic nutrients

검색결과 389건 처리시간 0.028초

Biosynthesis of Eudesmane-type Sesquiterpenoids by The Wood-rotting Fungus, Polyporus brumalis, on Specific Medium, including Inorganic Magnesium Source

  • Lee, Su-Yeon;Ryu, Sun-Hwa;Choi, In-Gyu;Kim, Myungkil
    • Journal of the Korean Wood Science and Technology
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    • 제44권2호
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    • pp.253-263
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    • 2016
  • Fungi, such as the wood-rotting Polyporus brumalis, are excellent sources of pharmaceutically interesting natural products such as sesquiterpenoids. In this study, we investigated the biosynthesis of P. brumalis sesquiterpenoids on modified medium. Ten additional species of white rot fungi were inoculated in medium containing nutrients such as $C_6H_{12}O_6$, $C_4H_{12}N_2O_6$, $KH_2PO_4$, $MgSO_4$, and $CaCl_2$ at $28^{\circ}C$ for 25 days. After 10 days of incubation, eudesmane-type sesquiterpenes, ${\beta}$-eudesmane and ${\beta}$-eudesmol, were only synthesized during the growth phase of P. brumalis. Experiments excluding one nutrient at a time were conducted to determine the effects of inorganic nutrients on sesquiterpene biosynthesis. In conclusion, GC-MS analysis showed that biosynthesis of sesquiterpenes was differentially regulated by inorganic nutrients such as $MgSO_4$, $C_4H_{12}N_2O_6$, and $KH_2PO_4$. We found $MgSO_4$ supplementation to be vital for eudesmane-type sesquiterpene biosynthesis in P. brumalis; nitrogen ($C_4H_{12}N_2O_6$) and phosphate ($KH_2PO_4$) inhibited the synthesis of P. brumalis metabolites. Magnesium is a known cofactor of sesquiterpene synthase, which promotes ${\beta}$-eudesmol synthesis. To mechanistically understand eudesmane-type sesquiterpene biosynthesis in P. brumalis, further research into the genes regulating the dynamics of such biosynthesis is warranted.

도시하천(갑천) 유역에서 수질오염의 공간적 특성 (Spatial Characterization of Water Pollution in the Urban Stream Watershed (Gap Stream), Korea)

  • 이흥수;허진;정선아;황순진;신재기
    • 한국물환경학회지
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    • 제22권5호
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    • pp.943-951
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    • 2006
  • Spatial distribution of water pollution in the Gap Stream was investigated from October to November, 2005. Sampling was conducted three times including effluents discharged from a wastewater treatment plant (WWTP) and a dam reservoir during the low-flow period. As a typical urban stream, total nitrogen and inorganic nitrogen concentrations increased toward downstream. Ammonia concentration was the highest in the treated water of the wastewater treatment plant and the lowest nitrate concentration was found in the effluent of the dam reservoir. A part of soluble reactive phosphorous (SRP) in total phosphorous was 22~54% in the upstream reach of WWTP in the Gap Stream whereas 68~73% in the downstream reach. Mean chlorophyll-a concentration ranged from 1.6 to $11.0{\mu}g/L$ and it tends to increase toward downstream except for WWTP effluent. As expected, untreated wastewater and WWTP effluent were suggested as the major sources of water pollution in the Gap Stream. In this study, the water pollution of the Gap Stream is a significant undergoing typical eutrophication, caused by excessive phosphorus and nitrogen nutrients from WWTP located in the watershed. As a result, the critical factor for the water pollution was evaluated to dissolved inorganic nitrogen and phosphorus nutrients. Particularly, SRP is a most important for the eutrophication. It suggest that may occur in the most urban streams of Korean peninsula. Therefore, because the necessity of water pollution management in the urban stream, inorganic N and P nutrients should be included as an essential component of water quality criteria in the advanced water quality project of Korean Government by enforcing of water quality assessment and total maximum daily loads (TMDLs).

시화호 해수유통량 증대에 따른 통계학적 수질 영향 분석 (A Statistical Assessment of Increasing Tidal Mixing Effects on Water Quality in the Shiwha Coastal Reservoir)

  • 이범연;이창희
    • 한국물환경학회지
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    • 제37권6호
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    • pp.425-432
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    • 2021
  • A tidal power plant (TPP) has been in operation since the end of 2011 to improve the water quality of Shihwa Coastal Reservoir (SCR). Tidal mixing rate increased 5.6 times after the TPP operation so that in this study, its effects on water quality was assessed through statistical analysis of long-term water quality monitoring data. It was found that the increased tidal mixing contributed to solving the hypoxia problem in the bottom water by preventing the summer stratification. The analysis also showed that the increased tidal mixing had different effects depending on the relative concentration difference for each water quality substances between the SCR and the outside of SCR. The average concentrations of some substances (chemical oxygen demand, total phosphorus, chlorophyll-a) with higher concentrations than the outside of SCR decreased due to the dilution effect, but the other substances (total nitrogen, dissolved inorganic nitrogen, dissolved inorganic phosphorus) with lower concentrations compared to the outside ones increased on the contrary. Factor analysis also showed a consistent result that the first factor accounting for the water quality was changed from the organic-related substances to the nutrient-related substances after the increased tidal mixing. These results imply that the focus of future water quality management needs shifting from the organic substances to the nutrients, particularly dissolved inorganic nutrients. Considering the effect of inflow seawater on the nutrients, the management area should be extended to cover not only SCR but also a certain area outside of SCR.

강원도 고성지역에서 산불이 소나무림 토양의 영양염류에 미치는 영향 (Effects of Forest Fire on Soil Nutrients in Pine Forests in Kosong Kangwon Province)

  • Mun, Hyeong-Tae;Choung, Yeon-Sook
    • The Korean Journal of Ecology
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    • 제19권5호
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    • pp.375-383
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    • 1996
  • Effects of forest fire on soil nutrients in pine forests were studied in Dosong, Kangwon Province in Korea. Soil samples were collected from burned sites and unburned sites (control) at Kusungri, Sampori and Wunbongri. Soil pH in burned sites was higher than that in unburned sites. Soil organic matter did not differ significantly between the burned and unburned sites. Concentrations of available phosphorus in burned soils were about 3 times higher than those in unburned sites. Also, concentrations of ammonium nitrogen were markedly increased in burned sites contrary to those of nitrate nitrogen. Total nitrogen, however, showed no significant difference between the sites. Concentrations of cations, calcium and magnesium, in burned sites were also significantly increased. Thus, even though forest fire removed the aboveground biomass of pine forests, inorganic nutrients added from ash may play a great role for growth of sprouts and seedlings after forest fire.

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섬진강 하구 염습지 갈대군락의 생산성과 토양양분의 계절적 변화 (Seasonal Changes in the Productivity and Soil Nutrients of Phragmites communis Community in the Salt Marsh of the Sumjin-River Estuary)

  • Oh, Kyung-Hwan;Ihm, Byung-Suh
    • The Korean Journal of Ecology
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    • 제6권2호
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    • pp.90-97
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    • 1983
  • Seasonal changes of the soil nutrient contents and aboveground biomass, relationship between the soil nutrients and the productivity, and the net efficiencies of solar energy conversion were studied in two reeed communities (Phragmites communis Trin.) at the salt marsh in the estuary of the Sumjin-River from April 30 to October 9, 1981. The inorganic nutrients such as exchangeable sodium and potassium of soil were decreased during growing season. The amounts of organic matter, exchangeable sodium and potassium, total nitrogen, and available phosphorus in stand $\prod$ were much more than those of stand $\coprod$ . Productivity of Phragmites communis was positively correlated with the soil nutrients such as available phosphorus, exchangeable potassium and total nitrogen. The maximum dry matter productions of the aboveground parts in stand $\prod$ stand $\coprod$ were $ 1, 120g/m^2; and; 843g/m^2$ in August, and the net coversion efficiencies of PhAR based on growing season (April to September) were 1.77% and 1.33%, respectively.

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돈분 처리에 의한 빈도리나무 유묘생육 및 무기양분 흡수에 관한 연구 (A Study on Seedling Growth and Absorption of Inorganic Nutrients of Deutzia Crenata Seedlings Treated with Swine Manure)

  • 이창헌;강학모;진재준;홍지숙;김미자
    • 한국환경복원기술학회지
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    • 제21권3호
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    • pp.1-12
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    • 2018
  • 본 연구는 빈도리나무를 대상으로 고형 돈분의 시비 농도에 따른 빈도리나무 종자의 발아율, 묘목의 생장량, 건중량, 체내 무기양분 흡수량 및 토양의 화학적 변화 등을 조사하여 빈도리나무 유묘의 생장에 고형 돈분의 적정 시용량에 대한 자료를 얻고자 실시하였다. 1. 고형 돈분 처리시 빈도리나무의 종자발아율은 대조구가 가장 높았으며, 고농도 돈분 처리시 발아율은 감소하는 경향을 나타냈다. 2. 묘의 생장량은 돈분 처리구가 대조구 보다 모두 높은 생장량을 보였고, 돈분 1.0% 처리구에서 묘고 생장이 가장 컸다. 3. 건중량은 1.0% 처리구에서 가장 높게 나타났고, 체내 무기양분 흡수량은 대부분 1.0% 처리구에서 높았으며, 2.0%에서 급격히 흡수량이 떨어졌다. 4. 빈도리나무 식재 토양의 경우 돈분을 고농도로 처리할수록 pH는 낮아지고, 토양 중 잔류된 전질소, 유효인산, 치환성 K, Na 및 Mg 함량은 높아졌다.

뿌리혹 선충기생 정도에 따른 인삼의 생육 및 무기성분 변화 (Effect of Root- Knot Nematode Infection on Ginseng Growth of Inorganic Nutrients in Ginseng Roots.)

  • 안용준;오승환
    • Journal of Ginseng Research
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    • 제7권1호
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    • pp.37-43
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    • 1983
  • Effect of root-knot nematode (Meloidigyne sp.) 1 infection on the growth and quantity of the inorganic substances in 6 year-old ginseng (Panax ginseng C.A. Meyer) was investigated. Naturally infected 6 year-old ginseng roots were grouped into 3 such as healthy, moderate, and heavy according to the degree of the nematode infection. The roots were sampled in the middle of October, 1980 for chemical analysis. Growth of roots in terms of fresh weight, dry weight, length and diameter was greatly reduced by the nematode infection. The content of inorganic substances was different among the various parts of ginseng roots and according to gall index. Phosphorous and nitrogen contents of the healthy ginseng roots were tend to higher than those of the infected ones, whereas calcium was high in infected roots in the order of heavy, moderate, and healthy. Content of the other inorganic substances was highest in the moderately infected roots, and lowest in the heavy one.

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늦여름 동해 남서해역에서 용존 유기 질소가 우점 식물플랑크톤의 생장에 미치는 영향 (Effects of Dissolved Organic Nitrogen on the Growth of Dominant Phytoplankton in the Southwestern Part of East Sea in Late Summer)

  • 권형규;전슬기;오석진
    • 해양환경안전학회지
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    • 제22권1호
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    • pp.42-51
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    • 2016
  • 2014년 9월 동해 남서해역의 용존 영양염 분포 및 식물플랑크톤 군집구조와 우점종의 질소 화합물에 대한 이용성을 파악하였다. 용존 무기 질소(dissolved inorganic nitrogen; DIN)와 용존 무기 인(dissolved inorganic phosphorus; DIP)은 표층에서 낮고, 수심이 깊어짐에 따라 증가하였다. 반면에 용존 유기 질소(dissolved organic nitrogen; DON)와 용존 유기 인의 경우 무기태 영양염과 상반되는 분포를 보였다. DIN:DIP 비는 전체 수괴에서 약 20으로 Redfield ratio (16)보다 다소 높게 나타났지만, 혼합층의 경우 2로 식물플랑크톤의 생장에 대해 무기 질소가 제한요인으로 작용할 수 있는 것으로 보였다. 특히, 무기 질소가 제한된 혼합층에서 DON은 용존 총 질소(dissolved total nitrogen; DTN) 중 88 %를 차지하였다. 우점종 Chaetoceros debilis와 Prorocentrum minimum은 DIN 이외에 요소와 아미노산과 같은 다양한 DON을 이용하여 생장하였다. 따라서 식물플랑크톤의 DON 이용능력은 DIN이 제한된 동해에서 중요한 생존전략으로 작용할 것이다.

생물검정에 의한 남조류 Microcystis가 수질에 미치는 영향 (The Impact on Water Quality from Blue-Green Algae Microcystis Natural Phytoplankton by Algal Assay)

  • 신재기;조경제
    • 한국환경과학회지
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    • 제9권3호
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    • pp.267-273
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    • 2000
  • In order to understand the impact for decomposition of blue-green algae Microcystis on water quality, the algae were cultivated with collection of natural population during approximately one month, when water-bloom of Microcystis dominated at August 31, 1999 in the lower part of the Okchon Stream. The enrichment of inorganic NㆍP nutrients didn't in algal assay and the effect of Microcystis on water duality was assessed from the variation of nutrients by algal senescence. Microcystis population seemed to play a temporary role of sink for nutrients in the water body. Initial algal density of Microcystis was 2.3×10/sup 6/ cells/㎖. When Microcystis population died out under light condition, algal NㆍP nutrients between 9∼12 days affected to increase of biomass after reuse by other algal growth as soon as release to the ambient water. However, cellular nutrients under dark condition were almost moved into the water during algal cultivation. NH₄, NO₃ and SRP concentration were highly increased with 160, 17 and 79 folds, respectively relative to the early. As a result, the senescence of Microcystis population seemed to be an important biological factor in which cause more eutrophy and increase of explosive algal development by a lot of nutrients transfer to water body. There are significantly observed an effort of reduce for production of inner organic matters such a phytoplankton as well as load pollutants from watershed in side of the water quality management of reservoir.

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수생식물을 이용한 담수 순환여과식 양식용수내의 무기영양염 처리 효율 (Inorganic Nutrient Removal Efficiency of Aquatic Plants from Recirculating Aquaculture System)

  • 마진석;오승용;조재윤
    • 한국양식학회지
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    • 제16권3호
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    • pp.171-178
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    • 2003
  • Inorganic nutrients such as nitrogen and phosphate compounds accumulate in recirculating aquaculture systems. These nutrients must be removed from the system before they affect pH and fish health. For this purpose, aquatic plants are a simple and inexpensive method of removal. There are four commonly used aquatic plants: Eichhornia crassipes (water hyacinth), Pistia stratiotes (water lettuce), Hygrophila angustifolia, and Hydrocotyle leucocephala in freshwater recirculating aquaculture systems in Korea, but their efficiencies are not known. Therefore, removal efficiencies of inorganic nutrients from a freshwater recirculating aquaculture water with four commonly used aquatic plants were tested. Removing efficiencies of TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N of the plants in 210 L aquaria for 48-hour period were tested. The removing efficiencies of TAN, N $O_3$$^{[-10]}$ -N, and P $O_4$$^{3-}$-P of the two most effective plants, water hyacinth and water lettuce, were also tested in 690 L (surface area of 1.55 $m^2$) tanks under 2 different initial stocking densities, 4 kg and 6 kg, for 22 days. Proximate analysis major nutrients and N and P contents of the all plants were analyzed for calculating net removal weight of N and P by the plants. Water lettuce was the most effective for removing TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N from the water for 48-hour period tested followed by water hyacinth and Hygrophila angustifolia. Water lettuce reduced TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N concentration from 2.3 mg/L, 0.197 mg/L, and 21.4 mg/L to 0.4 mg/L, 0.024 mg/L and 17.4 mg/L, respectively while water hyacinth reduced them down to 0.6 mg/L, 0.029 mg/L and 17.9 mg/L, respectively. The concentrations of TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N in Hydrocotyle leucocephala group were rather increased up to 3.7 mg/L, 5.7 mg/L and 48.2 mg/L, respectively. This is because the creeping stem of Hydrocotyle leucocephala had to be cut to meet stocking weight resulting in decaying of the stem in the aquaria during experiment. The net growth in weight of water hycinth and water lettuce of 4 kg each in the 1.55 $m^2$ tanks for 22 days were 9.768 kg and 10.803 kg respectively, and those at initial weight of 6 kg each were 8.393 kg and 9.433 kg, respectively. The reason of lower net growth in the later group was restricted growth space. Nitrogen and phosphorus contents in water hyacinth were 2.89% and 0.27%, and those in water lettuce were 3.87% and 0.36%, respectively. Average quantities of removed N and P from 1.55 $m^2$ tanks by water hyacinth for 22 days were 18.9 g and 1.75 g, while those by water lettuce were 36.8 g and 3.5 g, respectively. Therefore water lettuce showed much higher efficiencies for removing both N and P from recirculating aquaculture water than water hyacinth.