• Title/Summary/Keyword: inner shape

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Ultrastructure of Gametes in the Three-spine stickleback, Gasterosteus aculeatus aculeatus (큰가시고기 배우자의 미세구조)

  • Deung, Young-Kun;Kim, Dong-Heui;Reu, Dong-Suck
    • Applied Microscopy
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    • v.29 no.2
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    • pp.177-187
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    • 1999
  • Ultrastructure of gametes in the three-spine stickleback, Gasterosteus aculeatus aculeatus was observed, utilizing light, scanning and transmission electron microscopes. The egg of three-spine stickleback is spherical and demersal type. The eggs are highly adhesived to each other but not to substrates. There are many oil droplets in vitelline membrane. The outer surface of egg envelope is arranged by mushroom-like structures and pore canals. The egg have a micropyle, sperm entry site, in the area of the animal pole. The egg envelope consists of three layers, an outer layer with high electron density, a middle layer consisting two layers and an inner layer consisting of 16 to 20 layers. In the fertilized egg envelope, the molecular weights of these components ranged from 14 kDa to 205 kDa. The molecular weights of nam protein bands are 19.4 kDa, 36.7 KDa, 39.4 kDa, 42.9 kDa, 46.1 kDa and 53.0 kDa. The head of spermatozoa is spherical shape and the acrosome is absent. The mitochondria in midpiece are arranged from one to three layers and separated from the axoneme by the cytoplasmic canal. The tail has two lateral fins and the axoneme is of the 9+2 structure.

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The Oogenesis of Chinese minnow, Leuciscinae, Teleostei (경골어류 황어아과 버들치의 난자형성과정)

  • Kim, Dong-Heui;Chang, Byung-Soo;Jung, Han-Suk;Teng, Yung-Chien;Kim, Seok;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.39 no.3
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    • pp.237-243
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    • 2009
  • Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

Local Citizenry Consciousness Survey of 『Campaign for Shaping Mokpo into Beautiful Seaport』 1. Urban Environment and Citizenry Cultural Consciousness (미항목포가꾸기에 관한 지역시민의식 조사 1. 도시환경과 문화의식)

  • Kim Kwang Soo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.7 no.2
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    • pp.89-97
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    • 2004
  • Recently, Namak New Town is under construction adjacent to Mokpo City, and New Great Harbour Bridge between Mokpo City and Koha Island is designed to be built across Inner Harbour of Mokpo. Citizens' concerns about and participations in 「Campaign for Shaping Mokpo into Beautiful Seaport」 are required to make up the sight of Mokpo harbour beautifully. The of 「Master Plan for the Development of Beautiful Mokpo Seaport」 was reviewed. Citizens' levels of consciousness of and participation in 「Campaign for Shaping Mokpo into Beautiful Seaport」 were ascertained through questionnaire surveys of citizenry opinions, and prerequisites and requirements for the success of 「Master Plan for the Development of Beautiful Mokpo Seaport」 were suggested on the basis of the results of questionnaire surveys. As most of citizens are not satisfied with the existing urban environment in Mokpo City, the urban environment and the view of Mokpo are required to be made up according to the natural, social and environmental characteristics of Mokpo and its adjacent Islands in coastal waters. In order to cultivate citizens' basic cultural consciousness and sense of public kindness, cleanliness and orderliness, various educational programs are required to be developed and executed. Only when local government of Mokpo City collects the public opinions and continues to shape Mokpo into international beautiful seaport in the direction of a reflex of public opinion, Mokpo harbour can be made up into international beautiful seaport successfully with citizens' positive participation in citizenry practicable action plan.

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Local Citizenry Consciousness survey of 『Campaign for Shaping Mokpo into Beautiful Seaport』 2. Citizenry Consciousness of Marine Environment (미항목포가꾸기에 관한 지역시민의식 조사 2. 해양환경에 관한 시민의식)

  • Kim Kwang Soo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.7 no.2
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    • pp.98-103
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    • 2004
  • Recently, a New Great Harbour Bridge between Mokpo City and Koha Island is planned to be built across Mokpo Inner Harbour, and Namak New Town is under construction adjacent to Mokpo. Citizels' concorn of and participation in 「Campaign for Shaping Mokpo into Beautiful Seaport」 are necessary to match the view of Mokpo harbour with natural scenery beautifully and with marine environment harmoniously. Citizens'ㄹ levels of consciousness of coastal and marine environment were ascertained through questionnaire surveys of local citizenry attitudes and opinions, and citizenry action plans for the conservation of coastal and marine environments around Mokpo harbour are suggested on the basis of the results of questionnaire surveys. Citizens' voluntary participations in local policy for the control of marine wastes production and in a campaign for the collections of marine debris and wastes around beaches and coastal areas are required. The operation of municipal sewage treatment plants is to be performed effectively and efficiently, and to be monitored and evaluated periodically by the general public, the local federation for environmental movement or NGO. The development and performance of marine environmental education programs and an enlightenment campaign for environment preservation are to be incessantly done in cooperation with local residents. Only when local government of Mokpo collects the public opinions and continues to shape Mokpo into international beautiful seaport in the direction of a reflex of public opinion, coastal and marine environment around Mokpo harbour can be kept in good condition with citizens' positive participation in citizenry long-term practical action plans for environment conservation.

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A Study on the Droplet Formation of Liquid Metal in Water-Mercury System as a Surrogate of Molten Salt-Liquid Metal System at Room Temperature (용융염-액체금속 계의 대용물인 물-수은 계에서 액체금속 액적의 생성에 대한 연구)

  • Kim, Yong-il;Park, Byung Gi
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.16 no.2
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    • pp.165-172
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    • 2018
  • As an approach for estimation of the droplet size in the molten salt-liquid metal extraction process, a droplet formation experiment at room temperature was conducted to evaluate the applicability of the Scheele-Meister model with water-mercury system as a surrogate that is similar to the molten salt-liquid metal system. In the experiment, droplets were formed through the nozzle and the droplet size was measured using a digital camera and image analysis software. As nozzles, commercially available needles with inner diameters (ID) of 0.018 cm and 0.025 cm and self-fabricated nozzles with 3-holes (ID: 0.0135 cm), 4-holes (ID: 0.0135 cm), and 2-holes (ID: 0.0148 cm) were used. The mercury penetration lengths in the nozzles were 1.3 cm for the needles and 0.5 cm for the self-fabricated nozzles. The droplets formed from each nozzle maintained stable spherical shape up to 20 cm below the nozzle. The droplet size measurements were within a 10% error range when compared to the Scheele-Meister model estimates. The experimental results show that the Scheele-Meister model for droplet size estimation can be applied to nozzles that stably form droplets in a water-mercury system.

Determination of the Period of the Formation and Size of Sieve Element Area and Sieve Pore (Streptanthus tortus 조직배양 세포에서 사공의 형성시기와 사공 영역과 사공의 크기 결정)

  • Cho, Bong-Heuy
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.41-44
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    • 2002
  • During the phloem development from parenchyma cells in a suspension culture of Streptanthus induced sucrose carrier and glucose carrier disappeared. Sieve element area and sieve pore induced suspension culture of Streptanthus were formed almost at the last period of the synthesis of sieve endoplasmic reticulum (SER) and p-protein. The new synthesized cell wall begann to digeste only after the new cell wall was surrounded by SER. The digested region of the cell wall and the formed region of sieve pore were regular comparatively. The completed sieve pore was an oval form, and the outer portion of sieve pore varied, ca 1.2 ${\mu}{\textrm}{m}$~1.6 ${\mu}{\textrm}{m}$ in longitudinal, 0.8 ${\mu}{\textrm}{m}$~1.3 ${\mu}{\textrm}{m}$ in tangential, and the inner size of sieve pore was irregular form of a star-like shape. The number of sieve pore between sieve cells was ca 2~7 per ${\mu}{\textrm}{m}$$^2$ and the sieve pore wall with callose was 0.05 ${\mu}{\textrm}{m}$~0.07 ${\mu}{\textrm}{m}$ in thickness. The energy for the formation of sieve element area and sieve pore might be supplied by mitochondria near the new cell wall and the role of SER remains to be illucidated.

Histopathological observation of the gill of the crucian carp, Carassius auratus by the leech, Limnotrachelobdella sinensis (거머리, Limnotrachelobdella sinensis의 기생으로 인한 붕어, Carassius auratus 아가미의 조직병리학적 관찰)

  • Park, Myoung-Ae;Kim, Seok-Ryel;Kim, Myoung-Sug;Kim, Jeong-Ho;Park, Jung-Jun
    • Journal of fish pathology
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    • v.23 no.3
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    • pp.399-407
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    • 2010
  • On the inner side of each operculum of the crucian carp, Carassius auratus (n=10), the leech, Limnotrachelobdella sinensis of 1-4 individuals were parasitic. The leeches had approximately 41.0 mm in total length and 11 mm in width. These body was composed with anterior sucker, neck, trunk and posterior sucker and average length was 2.3 mm, 7.2 mm, 23.3 mm and 8.7 mm respectively. To both sides of the trunk lateral vesicle of 11 pair existed. When observed by SEM, anterior sucker was hemisphere shape and the mouth where proboscis comes out existed with the its center. Proboscis was connected the esophagus directly. Under light microscopy, bloodsucking gill of C. auratus showed lamella fusion, hypertrophy the epithelial cell of the filament and lamella, increased mucocytes and congested capillaries. On the other hand, necrotic and hydropic degeneration epithelial cell of the lamella, and infiltration of the macrophages from some individuals were suggested the secondary infection with the bacteria or virus after bloodsucking activity of the leech.

Reaction Force Analysis on the Journal Bearing of Shafting System with Contra-Rotating Propeller (상반회전 프로펠러 축계 저널 베어링 반력해석)

  • Shin, Sang-Hoon;Lee, Seung-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.10
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    • pp.270-276
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    • 2019
  • According to the International Maritime Organization (IMO) 2020 Regulation, ships operating outside designated emission control areas (ECA) have to use low-sulfur oil with a sulfur content of 0.5% or less by January 2020. To minimize the consumption of high-priced low-sulfur oil, it is urgent to introduce efficient energy-saving devices (ESD), and contra-rotating propeller (CRP) systems are well known to be the most effective one. The shafting system that drives a CRP is composed of an inner shaft and an outer one and has a mutually influential system that is much more complex and heavier than a general shafting system. An initial design was carried out to install a CRP system for the first time in Korea. The purpose of this study is to verify whether the journal bearing meets the classification's design criteria through a bearing reaction force analysis for the classification's approval of the initial design. It is ideal for the thrust of the propeller to act on the center of the shaft, but thrust eccentricity occurs due to the uneven wake caused by the stern shape. Load conditions were applied while considering thrust eccentricity to perform the shaft analysis, and the results were compared with the classification's criteria.

Taxonomy of Korean Asarum (Aristolochiaceae) by the Morphological Characters (한국산 족도리풀속(Asarum)의 외부형태학적 형질에 의한 분류)

  • Leem, Hyosun;Oh, Byoung-Un
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.344-354
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    • 2019
  • In seven species of Korean Asarum, 44 qualitative and 28 quantitative characters were reviewed taxonomically. Among 44 qualitative characters observed in vegetative and reproductive organs, the states of 27 characters were common in all the seven species. Among 28 quantitative characters, five characters have the same values in all the seven species; the number of cataphyll (2-4), leaf (2), calyx lobe (5), stamen (12) and pistil (6). White spots on adaxial surface of leaf (effectively) distinguished A. maculatum and A. versicolor from the other species. Many long soft hairs on abaxial surface of leaf distinguished A. mandshuricum from other species with short hairs. The glandular hairs on inner surface of calyx tube were divided into 2 types, reflecting the taxonomic relationships between the species: Type 1 has non-stalked glandular hairs (A. maculatum, A. sieboldii and A. sp.), and Type 2 has stalked glandular hairs (A. patens, A. versicolor, A. mandshuricum and A. misandrum). The length, shape, colour of calyx lobe and the extent to which the calyx lobe recurves, as well as the diameter and colour of calyx tube throat, were confirmed to be taxonomically valuable. In the case of glandular hair density on the surface of calyx lobe, A. patens with low density and A. sp. with high density were clearly distinct from all the other species with intermediate hair density. Meanwhile, the stylar protuberance length of A. patens (2.9-3.3 mm) was strikingly different from other species (1.4-2.5 mm). Additionally, further examination of the identities of A. yeonbyeonense and A. yeonbyeonense var. viridiluteolum is required.

Study on Modeling and Arrangement of Link-Shoes for Torsional Control of S-shaped Pedestrian Cable-Stayed Bridge (S자형 보도사장교의 비틀림 제어를 위한 링크슈의 모델링과 배치방법 연구)

  • Ji, Seon-Geun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.6
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    • pp.210-218
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    • 2019
  • Recently, cable-stayed bridges have been attempting to apply bold and experimental shapes for aesthetic and originality. In the case of bridges that have no similar cases, deep understanding and verification of analytical modeling is needed. S-shaped curved pedestrian cable-stayed bridge is always twisted because the cable is arranged on one side of the inverted triangular truss girder. In order to suppress the torsion, the Link-shoes are arranged at the left and right top members with reference to the Bearing placed at the mid-bottom member. The first research is related to the modeling method of Link-Shoe and Diaphram. In order to accurately reflect the transverse structural system and the torsional stiffness, it was necessary to model the Link-Shoe and the Diaphram directly rather than indirectly using the stiffness of the Bearing. The second study is related to the lateral arrangement of Bearing and Link-Shoes. Method 1 is to place in order of Link-shoe, Bearing, and Link-shoe from outside the curve radius. Method 2 is place to in order of Bearing, Bearing, and Link-shoe. In method 2, compared to method 1, the stress in the outer top member was larger and the stress in the inner one was decreased. It is analyzed that the stress adjustment is possible according to the lateral arrangement of Bearing and Link-Shoe.